PSAQ™ standards for accurate MS-based quantification of proteins: from the concept to biomedical applications

Improving the precision of quantitative bottom-up proteomics based on stable isotope-labeled proteins

(2012) Anna Konopka et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Biosimilar, Biobetter, and Next Generation Antibody Characterization by Mass Spectrometry

(2012) Alain Beck et al.   ANALYTICAL CHEMISTRY

Multiple-Reaction Monitoring-Mass Spectrometric Assays Can Accurately Measure the Relative Protein Abundance in Complex Mixtures

(2012) A. N. Hoofnagle et al.   CLINICAL CHEMISTRY

Introducing AAA-MS, a Rapid and Sensitive Method for Amino Acid Analysis Using Isotope Dilution and High-Resolution Mass Spectrometry

(2012) Mathilde Louwagie et al.   JOURNAL OF PROTEOME RESEARCH

Peptide Production and Decay Rates Affect the Quantitative Accuracy of Protein Cleavage Isotope Dilution Mass Spectrometry (PC-IDMS)

(2012) Christopher M. Shuford et al.   MOLECULAR & CELLULAR PROTEOMICS

Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions

(2012) Paola Picotti et al.   NATURE METHODS

Mass spectrometry-based absolute protein quantification: PSAQ™ strategy makes use of “noncanonical” proteotypic peptides

(2012) Michel Jaquinod et al.   PROTEOMICS

Consensus Statement on the Standardization and Evaluation of Growth Hormone and Insulin-like Growth Factor Assays

(2011) D. R. Clemmons et al.   CLINICAL CHEMISTRY

Selected reaction monitoring applied to proteomics

(2011) Sebastien Gallien et al.   JOURNAL OF MASS SPECTROMETRY

SILAC Compatible Strain ofPichia pastorisfor Expression of Isotopically Labeled Protein Standards and Quantitative Proteomics

(2011) Ryan J. Austin et al.   JOURNAL OF PROTEOME RESEARCH

Absolute SILAC-Compatible Expression Strain Allows Sumo-2 Copy Number Determination in Clinical Samples

(2011) Ivan Matic et al.   JOURNAL OF PROTEOME RESEARCH

Development of a Protein Standard Absolute Quantification (PSAQ™) assay for the quantification of Staphylococcus aureus enterotoxin A in serum

(2011) Annie Adrait et al.   Journal of Proteomics

Comparability analysis of protein therapeutics by bottom-up LC-MS with stable isotope-tagged reference standards

(2011) Anton V. Manuilov et al.   mAbs

The importance of the digest: Proteolysis and absolute quantification in proteomics

(2011) Philip Brownridge et al.   METHODS

Accurate Quantification of Cardiovascular Biomarkers in Serum Using Protein Standard Absolute Quantification (PSAQ™) and Selected Reaction Monitoring

(2011) Céline Huillet et al.   MOLECULAR & CELLULAR PROTEOMICS

Interlaboratory Evaluation of Automated, Multiplexed Peptide Immunoaffinity Enrichment Coupled to Multiple Reaction Monitoring Mass Spectrometry for Quantifying Proteins in Plasma

(2011) Eric Kuhn et al.   MOLECULAR & CELLULAR PROTEOMICS

A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines

(2011) Marlis Zeiler et al.   MOLECULAR & CELLULAR PROTEOMICS

Protein standard absolute quantification (PSAQ) method for the measurement of cellular ubiquitin pools

(2011) Stephen E Kaiser et al.   NATURE METHODS

Considering the advantages and pitfalls of the use of isotopically labeled protein standards for accurate protein quantification

(2011) Caroline Pritchard et al.   Bioanalysis

Simultaneous Quantification of Apolipoprotein A-I and Apolipoprotein B by Liquid-Chromatography-Multiple- Reaction-Monitoring Mass Spectrometry

(2010) S. A. Agger et al.   CLINICAL CHEMISTRY

Critical comparison of MS and immunoassays for the bioanalysis of therapeutic antibodies

(2010) Eric Ezan et al.   Bioanalysis

Stable-isotope dilution LC–MS for quantitative biomarker analysis

(2010) Eugene Ciccimaro et al.   Bioanalysis

Absolute Quantification of Phosphorylation on the Kinase Activation Loop of Cellular Focal Adhesion Kinase by Stable Isotope Dilution Liquid Chromatography/Mass Spectrometry

(2009) Eugene Ciccimaro et al.   ANALYTICAL CHEMISTRY

Full Dynamic Range Proteome Analysis of S. cerevisiae by Targeted Proteomics

(2009) Paola Picotti et al.   CELL

Quantification of Serum 1-84 Parathyroid Hormone in Patients with Hyperparathyroidism by Immunocapture In Situ Digestion Liquid Chromatography-Tandem Mass Spectrometry

(2009) V. Kumar et al.   CLINICAL CHEMISTRY

Perspectives of targeted mass spectrometry for protein biomarker verification

(2009) Ruth Hüttenhain et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Targeting Interferon Alpha Subtypes in Serum: A Comparison of Analytical Approaches to the Detection and Quantitation of Proteins in Complex Biological Matrices

(2009) Anita Izrael-Tomasevic et al.   JOURNAL OF PROTEOME RESEARCH

FLEXIQuant: A Novel Tool for the Absolute Quantification of Proteins, and the Simultaneous Identification and Quantification of Potentially Modified Peptides

(2009) Sasha Singh et al.   JOURNAL OF PROTEOME RESEARCH

Isotope dilution strategies for absolute quantitative proteomics

(2009) Virginie Brun et al.   Journal of Proteomics

Proteome-wide cellular protein concentrations of the human pathogen Leptospira interrogans

(2009) Johan Malmström et al.   NATURE

Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma

(2009) Terri A Addona et al.   NATURE BIOTECHNOLOGY

Towards Absolute Quantification of Therapeutic Monoclonal Antibody in Serum by LC−MS/MS Using Isotope-Labeled Antibody Standard and Protein Cleavage Isotope Dilution Mass Spectrometry

(2008) Olivier Heudi et al.   ANALYTICAL CHEMISTRY

The bacterial superantigen and superantigen-like proteins

(2008) John D. Fraser et al.   IMMUNOLOGICAL REVIEWS

Absolute SILAC for Accurate Quantitation of Proteins in Complex Mixtures Down to the Attomole Level

(2008) Stefan Hanke et al.   JOURNAL OF PROTEOME RESEARCH

Selected reaction monitoring for quantitative proteomics: a tutorial

(2008) Vinzenz Lange et al.   Molecular Systems Biology

Production of membrane proteins using cell-free expression systems

(2008) Daniel Schwarz et al.   PROTEOMICS

Protein Standard Absolute Quantification (PSAQ) for improved investigation of staphylococcal food poisoning outbreaks

(2008) Alain Dupuis et al.   PROTEOMICS

The interface between biomarker discovery and clinical validation: The tar pit of the protein biomarker pipeline

(2008) Amanda G. Paulovich et al.   Proteomics Clinical Applications

Observation of heterogeneous gene products by FT-ICR MS

(2007) Duncan H. L. Robertson et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY