Journal
JOURNAL OF MASS SPECTROMETRY
Volume 47, Issue 2, Pages 237-241Publisher
WILEY
DOI: 10.1002/jms.2049
Keywords
MALDI; Imaging; Sucrose; Cryo-protection; Eye; Histology
Funding
- EPSRC (through the PSIBS doctoral training centre)
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Sucrose is used as a cryo-preservation agent on large mammalian eyes post formalin fixation and is shown to reduce freezing artefacts allowing the collection of 12-mu m thick sections from these large aqueous samples. The suitability of this technique for use in MALDI imaging experiments is demonstrated by the acquisition of the first images of lipid distributions within whole sagittal porcine eye sections. Copyright (C) 2012 John Wiley & Sons, Ltd.
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