Journal
JOURNAL OF MASS SPECTROMETRY
Volume 44, Issue 7, Pages 1047-1052Publisher
WILEY
DOI: 10.1002/jms.1580
Keywords
Amadori rearrangement; glycation; ECD; sequencing
Funding
- Ministry of Science and Higher Education of Poland [N N401 222734, 3 T09A 029 28]
Ask authors/readers for more resources
The electron capture dissociation (ECD) of peptide-derived Amadori products has been successfully applied for their sequencing. In contrast to the collision induced dissociation (CID), based on the vibrational excitation of peptides, the ECD method does not produce ions formed by fragmentation of the hexose moiety, that facilitates interpretation of the obtained spectra. The fragmentation spectrum is dominated by c(n) and z center dot(n) ions, providing the sufficient information for sequencing of peptides and establishing the location of glycated Lys residues in the peptide chain. The ECD experiments were conducted on a series of synthetic peptides and unseparated digests of glycated ubiquitin. Copyright (C) 2009 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available