Article
Chemistry, Applied
Boyuan Guan, Zhenghan Zhang, Xiaoyu Liu, Shanshan Zhao, Xue Bai, Xue Luo, Daguang Feng, Liu Yang, Xueyan Cao, Xiqing Yue
Summary: In this study, the site-specific N-glycosylation of human MFGM proteins during lactation was characterized and quantified using a glycopeptide-centered strategy. The results revealed dynamic changes in the N-glycosylation patterns of MFGM proteins, with certain N-glycoproteins and their specific sites showing significant increases or decreases during milk maturation. Furthermore, specific glycan structures in human MFGM glycoproteins were found to play a role in antigen processing and presentation. These findings deepen our understanding of site-specific N-glycosylation of MFGM proteins and provide a background reference for the development of infant formulas.
CARBOHYDRATE POLYMERS
(2023)
Article
Chemistry, Applied
Huiwen Zhao, Mohan Li, Qing Zhu, Aicheng Liu, Jiayang Bi, Zhizhong Quan, Xue Luo, Yan Zheng, Ning Yang, Xiqing Yue, Xueyan Cao
Summary: This study used label-free quantitative proteomics to analyze and compare milk fat globule membrane (MFGM) proteins in porcine colostrum and porcine mature milk. A total of 3917 and 3966 MFGM proteins were identified in colostrum and mature milk, respectively. Of these, 3807 proteins were common to both groups, with 303 proteins showing significant differential expression. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses revealed the functions and pathways associated with the differentially expressed MFGM proteins. These findings provide crucial insights into the functional diversity of MFGM proteins in porcine milk and offer theoretical guidance for future research and development.
Article
Food Science & Technology
Zhen Feng, Yan Shen, Gongjian Fan, Tingting Li, Caie Wu, Yuhui Ye
Summary: This study reveals the subtle differences in protein compositions of bovine colostrum, first lactation milk, and second lactation milk, contributing to the development of protein ingredients for infant formula.
Article
Chemistry, Applied
Boyuan Guan, Zhenghan Zhang, Xueyan Cao, Mei Yang, Yuxia Chai, Xiakouna Amantai, Xue Luo, Daguang Feng, Yiming Liu, Xiqing Yue, Xiaoyu Liu
Summary: This study utilized a specific glycoproteomics strategy to investigate the site-specific N-glycosylation of donkey and human milk fat globule membrane (MFGM) proteins. The results showed differences and dynamic changes in the glycosylation patterns, deepening our understanding of the composition and physiological roles of donkey and human MFGM glycoproteins.
Article
Immunology
Julia Chronopoulos, James G. Martin, Maziar Divangahi
Summary: During pregnancy, the immune system of pregnant women and their offspring must find a balance between fetal tolerance and protection against pathogens. This delicate balance often leads to increased susceptibility to infections. In this study, it was found that offspring from mice infected with influenza A virus (IAV) were significantly protected against IAV infection, thanks to the elevated levels of maternal serum IgG1. The enhanced host resistance was only observed in mice born to and nursed by IAV-infected mothers, indicating the importance of maternal IgG1 from both the placenta and breast milk in providing prolonged protection against IAV infection in offspring.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Agriculture, Dairy & Animal Science
Ryo Inoue, Takamitsu Tsukahara
Summary: Colostrum, the first secretion of mammary glands from sows 24 hours post parturition, is crucial for the health and survival of piglets. It is high in protein, low in lactose and fat, containing immunoglobulins and bioactive proteins that support the development of the piglet's immune system.
ANIMAL SCIENCE JOURNAL
(2021)
Article
Agriculture, Dairy & Animal Science
Muhammad Malik, Muhammad A. Rashid, Didier Raboisson
Summary: Calves rely on the ingestion and absorption of colostrum for immune defense. Heat treatment of colostrum can reduce bacterial count and affect the levels of immune substances. Meta-analysis shows that heat-treated colostrum decreases the levels of immune substances in colostrum and has an impact on the levels of immune substances in serum.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Yufang Liu, Junna Cai, Fuxin Zhang
Summary: The study found that goat colostrum and mature milk have different effects on the intestinal microbiota, with colostrum containing richer nutrients that may promote the growth of both probiotics and pathogens, while mature milk can increase the abundance of probiotics.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Food Science & Technology
Zhigao An, Gan Luo, Shanshan Gao, Xinxin Zhang, Chao Chen, Zhiqiu Yao, Junwei Zhao, Haimiao Lv, Kaifeng Niu, Pei Nie, Liguo Yang
Summary: This study aimed to evaluate the differences between buffalo colostrum and mature milk. The results showed that colostrum had higher levels of protein, total solids, solid not fat, and milk urea nitrogen, while fat and lactose concentrations were lower compared to mature milk. Furthermore, colostrum had higher concentrations of certain minerals such as sodium, magnesium, cobalt, iron, and potassium with a lower concentration of calcium. The characteristics of colostrum were affected by parity, and the composition of milk and minerals changed significantly as lactation proceeded.
Article
Chemistry, Applied
Mohan Li, Jiali Chen, Xinyu Shen, Rayhnigul Abdlla, Lifei Liu, Xiqing Yue, Qilong Li
Summary: This study analyzed the metabolites in colostrum and mature breast milk using metabolomics techniques. The results identified 72 differentially expressed metabolites between the two types of milk and revealed metabolic pathways related to infant growth and development. These findings provide a theoretical basis for optimizing infant formula.
Article
Agriculture, Dairy & Animal Science
A. J. Lopez, M. A. Steele, M. Nagorske, R. Sargent, D. L. Renaud
Summary: The study evaluated the accuracy of using STP measurements to estimate FTPI in calves fed colostrum replacer (CR) compared to those fed maternal colostrum. Results showed that STP and IgG concentrations were highly correlated in calves fed maternal colostrum, but lowly correlated in calves fed CR. The current cutoff points for STP may overestimate the number of calves with FTPI when fed CR.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Wenyuan Zhang, Jing Lu, Baorong Chen, Peng Gao, Bo Song, Shuwen Zhang, Xiaoyang Pang, Kasper Hettinga, Jiaping Lyu
Summary: In this study, milk samples from 10 cows in different lactation stages were collected to investigate the differential expression of whey proteins and N-glycoproteins. A total of 240 whey proteins and 315 N-glycosylation sites on 214 glycoproteins were identified. GO annotations, KEGG pathway analysis, and protein classification were conducted to understand the similarities and differences in biological functions between whey proteins and N-glycoproteins across different lactation stages. The study also found differential expression of whey proteins and whey N-glycosylated proteins between lactation stages, and discussed the related changes in biological functions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Maria Moessler, Janina Aichner, Anja Mueller, Thiemo Albert, Thomas Wittek
Summary: This study examined the composition of alpaca colostrum and milk over the first four days after birth and the first four months of lactation. Differences in fat, protein, lactose, and mineral concentrations were observed between colostrum and milk, with significant changes in these indicators during the first four days after birth.
Article
Biochemistry & Molecular Biology
Liqiang Han, Qixue Huang, JingNa Yang, Wenyan Lu, Mingyue Hu, Yanbin Yang, Heshui Zhu, Kun Pang, Guoyu Yang
Summary: Milk fat in mammary epithelial cells is synthesized and secreted as lipid droplets in the form of milk fat globules (MFGs) covered by a lipid-protein membrane called the milk fat globule membrane (MFGM). This study investigated the effects of MFGM proteins from control and conjugated linoleic acid (CLA)-treated milk on lipid droplet formation in MAC-T cells. Results showed that CLA reduced milk fat content and MFG size parameters, while increasing the specific surface area (SSA). Proteomic analysis identified differentially expressed proteins in the CLA group, and CLA-MFGM proteins promoted lipid droplet fusion and influenced droplet size distribution in MAC-T cells.
Article
Nutrition & Dietetics
Jing Lu, Wenyuan Zhang, Changlu Ma, Xiaoyang Pang, Ying Dai, Tong Zhu, Jinqi Liu, Lina Xing, Shuwen Zhang, Jiaping Lv
Summary: In this study, a total of 998 proteins and 764 glycosylated sites from 402 glycoproteins were identified in human milk by TMT labeling proteomics. The glycoproteins were mainly enriched in cell adhesion, proteolysis, and defense/immune process. Quantitative analysis revealed that 78 glycosylated sites in 56 glycoproteins and 10 glycosylated sites in 10 glycoproteins were significantly upregulated in colostrum and mature milk, respectively, and these changes were mainly related to host defense.
FRONTIERS IN NUTRITION
(2023)
