Journal
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS
Volume 321, Issue 3, Pages 192-197Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jmmm.2008.08.104
Keywords
Cell uptake; Viability; Cytotoxicity; Maghemite nanoparticle; Giant magneto-impedance bio-sensor
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In this work we determine conditions to produce cell samples for imaging with detection of the modi. cation of the magnetic field by maghemite (Fe2O3) nanoparticles acting as a high sensitivity magnetic bio-sensor based on the giant magneto-impedance (GMI) effect. Mat Ly Lu cells are grown for 24 h with various maghemite nanoparticles concentrations (from 0 to 6mg/ml). The percentage of viable cells is determined by counting labeled cells with trypan blue under an optical microscope. The quantity of nanoparticles internalized into the cells is evaluated by X-ray fluorescence analysis and expressed in iron moles per cell. The GMI bio-sensor was tested with the various samples. We observed that the best sensitivity of the GMI bio-sensor was obtained at a frequency of 1MHz. To confirm these results in the presence of cell samples, four measurement frequencies were pre-selected (from 1 to 100MHz) and tested. Cell growth conditions compatible with an acceptable percentage of cell viability for various concentrations of nanoparticles were also determined. These experiments allow us to conclude that cell growth with 0.1 mg/ml of nanoparticles for 24 h shows modi. cations of the magnetic field detectable optimally at 1MHz frequency. (C) 2008 Elsevier B.V. All rights reserved.
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