Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 196, Issue 2, Pages 157-163Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2008.11.001
Keywords
[1,2-C-13(2)]acetate; [1,6-C-13(2)]glucose; C-13 MRS, brain; Isotopomers; Neuronal-glial metabolism
Funding
- NIH [P41 RR008079, P30 NS057091, R01 NS038672]
- Keck Foundation
- University of Minnesota Medical School, NSF [BIR-961477]
- Minnesota Medical Foundation
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In this work the feasibility of measuring neuronal-glial metabolism in rat brain in vivo using co-infusion of [1,6-C-13(2)]glucose and [1,2-C-13(2)]acetate was investigated. Time courses of C-13 spectra were measured in vivo while infusing both C-13-labeled substrates Simultaneously. Individual C-13 isotopomers (singlets and multiplets observed in C-13 spectra) were quantified automatically using LCModel. The distinct C-13 spectral pattern observed in glutamate and glutamine directly reflected the fact that glucose was metabolized primarily in the neuronal compartment and acetate in the glial compartment. Time courses of concentration of singly and multiply-labeled isotopomers of glutamate and glutamine were obtained with a temporal resolution of 11 min. Although dynamic metabolic modeling of these C-13 isotopomer data will require further work and is not reported here, we expect that these new data will allow more precise determination of metabolic rates as is Currently possible when using either glucose or acetate as the sole C-13-labeled substrate. (C) 2008 Elsevier Inc. All rights reserved.
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