Article
Immunology
Jacob F. Rehring, Triet M. Bui, Carlos Samuel Galan-Enriquez, Jessica M. Urbanczyk, Xingsheng Ren, Hannah L. Wiesolek, David P. Sullivan, Ronen Sumagin
Summary: MPO plays a crucial role in modulating PMN trafficking in inflammation, with MPO knockout mice showing significantly enhanced inflammatory PMN tissue accumulation due to increased migratory ability.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Silvie Kremserova, Anna Kocurkova, Michaela Chorvatova, Anna Klinke, Lukas Kubala
Summary: The study reveals that MPO plays a role in regulating PMN cell death by enhancing cell membrane permeabilization and phosphatidylserine surface exposure, thereby extending the lifespan of PMNs.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Paulina Valadez-Cosmes, Sofia Raftopoulou, Zala Nikita Mihalic, Gunther Marsche, Julia Kargl
Summary: Myeloperoxidase is an important enzyme found in neutrophils that functions as a catalyzer of reactive oxidants to clear pathogens. It also has non-canonical functions and may play a role in inflammatory diseases and cancer development.
PHARMACOLOGY & THERAPEUTICS
(2022)
Review
Biochemistry & Molecular Biology
Ankita Tulangekar, Tamar E. Sztal
Summary: Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder caused by mutations in the dystrophin gene. Lack of functional dystrophin protein leads to fragile muscle membranes and increased susceptibility to damage during contraction. Inflammation plays a crucial role in exacerbating muscle damage and impairing regeneration in DMD patients, with neutrophils releasing inflammatory compounds that prolong the inflammatory response.
Article
Engineering, Biomedical
Shiyi Huang, Sijie Li, Yanlei Liu, Behafarid Ghalandari, Ling Hao, Chengjie Huang, Wenqiong Su, Yuqing Ke, Daxiang Cui, Xiao Zhi, Xianting Ding
Summary: The study reveals the different defensive behaviors of neutrophils towards graphene oxide (GO) and their mechanisms, including NETosis induced by mGO and degranulation triggered by nGO, accompanied by the generation of reactive oxygen species and activation of p-ERK and p-Akt kinases. Moreover, MPO plays a determinant role in the different responses of neutrophils to GO.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
B. P. Yakimov, I. I. Vlasova, Y. M. Efremov, E. G. Maksimov, E. A. Shirshin, V. E. Kagan, P. S. Timashev
Summary: Research shows that monitoring the degradation of biomaterials using fluorescence lifetime imaging (FLIM) can provide fast, accurate, and non-invasive assessments. FLIM can be used as a marker for oxidative degradation and is correlated with the micromechanical properties of scaffolds.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Kaushalya Amunugama, Grant R. Kolar, David A. Ford
Summary: Neutrophils are the most abundant white blood cells recruited to sites of infection and inflammation and their activation can lead to chlorolipid production. Studying two different strains of E. coli in coculture with neutrophils revealed differences in chlorolipid profiles, as well as bactericidal activity. These findings suggest that neutrophil-derived chlorolipid production varies in response to different strains of E. coli bacteria.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Po Ying Chia, Andrew Teo, Tsin Wen Yeo
Summary: The study found that cardiac function was impaired and levels of myeloperoxidase were higher in patients with severe dengue, suggesting that myeloperoxidase may mediate dengue-associated cardiac impairment.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Kai Yao, Qingchun Mu, Yufei Zhang, Qian Cheng, Xiaozhi Cheng, Xiaojing Liu, Chunmei Luo, Chumei Li, Shuxia Cai, Zhicheng Luo, Xiulong Zhu, Xiangtong Zhang, Liao Cui, Chunming Huang, Longguang Tang
Summary: In this study, a nanodrug targeting neutrophils and myeloperoxidase (MPO) was designed to enhance the retention and sustained release of drug cargos for improved traumatic brain injury (TBI) therapy. The findings demonstrated that the neutrophil-targeting nanoparticles effectively inhibited neuroinflammation and improved neurological deficits through sustained release of the drug in the brain.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Immunology
Marcin Zawrotniak, Magdalena Juszczak, Justyna Mosio-Wojcik, Maria Rapala-Kozik
Summary: Neutrophil extracellular traps (NETs) are structures released by neutrophils in response to various infections, and they have been found to be present in nasal mucus of patients with infectious rhinosinusitis. However, the DNA content was not identified in samples from allergic patients. This study suggests that the composition of nasal mucus varies according to the cause of mucosal irritation and further research is needed to explore the potential of NETs as a therapeutic target in respiratory diseases.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Caleb Nwongbouwoh Muefong, Olumuyiwa Owolabi, Simon Donkor, Salome Charalambous, Joseph Mendy, Isatou C. M. Sey, Abhishek Bakuli, Andrea Rachow, Christof Geldmacher, Jayne S. Sutherland
Summary: This study revealed that in patients with pulmonary tuberculosis, severe lung pathology at baseline and persistent lung damage after treatment were associated with higher levels of major pro-inflammatory neutrophil-derived mediators. Additionally, low sputum MPO levels were correlated with severe lung damage, higher Mtb burden, and poor recovery.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Z. N. Mihalic, T. Kloimboeck, N. Cosic-Mujkanovic, P. Valadez-Cosmes, K. Maitz, O. Kindler, C. Wadsack, A. Heinemann, G. Marsche, M. Gauster, J. Pollheimer, J. Kargl
Summary: This study reveals that internalization and activity of myeloperoxidase (MPO) enhances migration and invasion of JEG-3 cells, suggesting a potential role of MPO in both normal and abnormal pregnancies.
Article
Biochemistry & Molecular Biology
Shubha Shakya, Reagan M. McGuffee, David A. Ford
Summary: Hypochlorous acid is formed when leukocyte myeloperoxidase attacks the phospholipid plasmalogen molecular subclass, leading to the formation of 2-chlorofatty aldehydes (2-ClFALDs). The biological role of 2-ClFALD is not well understood. In this study, the effects of 2-ClHDA, an analog of 2-ClFALD, on the metabolic activity of RAW 264.7 cells were investigated. It was found that 2-ClHDA reduced the metabolic activity of the cells in a dose-dependent manner. Furthermore, N-acetyl cysteine (NAC) was shown to reduce the toxic effects of 2-ClFALD by reducing protein modification.
Article
Veterinary Sciences
Nazare Storms, Carlos Medina Torres, Thierry Franck, Albert Sole Guitart, Geoffroy de la Rebiere, Didier Serteyn
Summary: This study aimed to observe whether myeloperoxidase is increased in the laminae during early stage laminitis in horses subjected to prolonged euglycemic hyperinsulinemia. The results showed that neutrophil myeloperoxidase release may contribute to the pathophysiology of endocrinopathic laminitis.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Lara C. Linnemann, Ulrich E. Schaible, Tobias K. Dallenga
Summary: Host-directed therapies targeting immune pathways associated with pathogenesis in tuberculosis may improve disease and bacterial clearance. However, the efficacy of these therapies may vary between human and mouse models, as mouse models may not fully resemble the pathomechanisms of human tuberculosis. In this study, inhibition of myeloperoxidase (MPO) failed to prevent necrosis in mouse neutrophils infected with M. tuberculosis, in contrast to the previous findings in human neutrophils. These results suggest that murine infection models may not be suitable for evaluating host-directed therapies targeting neutrophils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Takuma Kobayashi, Takeshi Toyoda, Yuya Tajima, Shinji Kishimoto, Yuta Tsunematsu, Michio Sato, Kohei Matsushita, Takanori Yamada, Yuko Shimamura, Shuichi Masuda, Masako Ochiai, Kumiko Ogawa, Kenji Watanabe, Takeji Takamura-Enya, Yukari Totsuka, Keiji Wakabayashi, Noriyuki Miyoshi
Summary: The study suggests that a novel metabolite MxMxBD may increase the risk of bladder carcinogenesis by exhibiting stronger mutagenicity and genotoxicity compared to o-Ans. Additionally, heterodimerizations were observed when o-Ans was coincubated with o-Tol or aniline, highlighting the importance of considering both homodimerizations and heterodimerizations in evaluating the combined exposure risk of bladder carcinogenesis.
CHEMICAL RESEARCH IN TOXICOLOGY
(2021)
Article
Chemistry, Applied
Yoji Kato, Yui Kishi, Yayako Okano, Masaki Kawai, Michiyo Shimizu, Naoko Suga, Chisato Yakemoto, Mai Kato, Akika Nagata, Noriyuki Miyoshi
Summary: Reports on the thermal stability of manuka honey in terms of food processing have been limited. This study found that heating resulted in significant decreases in MGO and MAP in manuka honey. The mechanism for the decrease in MGO involves an amino-carbonyl reaction, while the decrease in MAP is influenced by the volume of headspace in a vessel. Additionally, heating can cause signature molecules in honey to vaporize.
Article
Biochemistry & Molecular Biology
Hiroki Matsuyama, Wataru Tanaka, Noriyuki Miyoshi, Tatsuo Miyazaki, Hideyuki Michimoto, Hiroyuki Sakakibara
Summary: The study evaluated the effects of daily consumption of raw or sun-dried radishes on apolipoprotein E-deficient mice. Consumption of both diets led to decreased body weight gain, visceral fat weight, and serum triglyceride levels in the mice. Additionally, oral fat tolerance tests showed that pretreatment with the radish diets mitigated the increase in serum triglyceride levels after oil administration.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Tao Zhou, Yuichiro Hirayama, Yuta Tsunematsu, Nanami Suzuki, Seiji Tanaka, Nahoko Uchiyama, Yukihiro Goda, Yuko Yoshikawa, Yuji Iwashita, Michio Sato, Noriyuki Miyoshi, Michihiro Mutoh, Hideki Ishikawa, Haruhiko Sugimura, Keiji Wakabayashi, Kenji Watanabe
Summary: The study successfully identified various derivatives of Colibactin produced by a high-Colibactin-producing strain, providing insights into the metabolic pathways and potential DNA adducts of Colibactin, which could help in diagnosing colorectal cancer.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Microbiology
Daiki Watanabe, Haruka Murakami, Harumi Ohno, Kumpei Tanisawa, Kana Konishi, Kikue Todoroki-Mori, Yuta Tsunematsu, Michio Sato, Yuji Ogata, Noriyuki Miyoshi, Naoto Kubota, Jun Kunisawa, Keiji Wakabayashi, Tetsuya Kubota, Kenji Watanabe, Motohiko Miyachi
Summary: This study found that stool patterns may help evaluate the presence of tumorigenic bacteria and fecal fatty acids without the need for specialist technology or skill. Specifically, a certain stool pattern was found to be associated with pks(+)E. coli and had significant correlations with specific fecal fatty acids.
Article
Multidisciplinary Sciences
Nanami Senoo, Takumi Akahori, Hiyori Ichida, Noriyuki Miyoshi, Akihito Morita, Takao Shimizu, Hideo Shindou, Shinji Miura
Summary: The study found that fasting led to a decrease in 22:6-phosphatidylcholines (PCs) and an increase in 18:2-PCs in skeletal muscle phospholipid profiles. This increase in 18:2-PCs during fasting was able to compensate for the loss of 22:6-PCs, maintaining the total amount of polyunsaturated fatty acid (PUFA)-containing PCs. Similar phospholipid alterations were observed in insulin-deficient mice, indicating that these perturbations were characteristic of catabolic skeletal muscle. In muscles lacking 22:6-PCs, such as lysophosphatidic acid acyltransferase 3 knockout mice, accumulation of other PUFA-containing PCs, mainly 18:2-PCs, suggests a compensatory mechanism for maintaining PUFA-containing PCs in skeletal muscle.
Review
Food Science & Technology
Tomokazu Ohishi, Sumio Hayakawa, Noriyuki Miyoshi
Summary: Epidemiological studies have shown that consumption of green tea, coffee, wine, and curry may reduce the risk of various cancers, although the effects can differ depending on the specific cancer site. Compounds found in these foods, such as chlorogenic acid, curcumin, epigallocatechin gallate, and resveratrol, can act as antioxidants or prooxidants to modulate reactive oxygen species (ROS) and miRNA expression, thereby exerting anticancer effects.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Review
Biochemistry & Molecular Biology
Yasukiyo Yoshioka, Tomokazu Ohishi, Yoriyuki Nakamura, Ryuuta Fukutomi, Noriyuki Miyoshi
Summary: Consumption of coffee, tea, wine, curry, and soybeans has been linked to a lower risk of cancer. Polyphenols play a major role in the anticancer effects of these foods, influencing ROS-mediated pathways and miR expression. Further research can provide more convincing evidence.
Article
Chemistry, Medicinal
Takuma Kobayashi, Shinji Kishimoto, Shogo Watanabe, Yasukiyo Yoshioka, Takeshi Toyoda, Kumiko Ogawa, Kenji Watanabe, Yukari Totsuka, Keiji Wakabayashi, Noriyuki Miyoshi
Summary: This study identifies the formation of several p-semidine-type dimers, including MMBD and MxMxBD, during the metabolic conversion of o-Tol and o-Ans. The dimers containing the o-Ans moiety showed faster dimerization rates but decreased levels with prolonged incubation. These findings suggest the potential significance of the homo-and hetero-dimers of monocyclic aromatic amines in bladder carcinogenesis and the combined exposure risk.
CHEMICAL RESEARCH IN TOXICOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Tomokazu Ohishi, Noriyuki Miyoshi, Mari Mori, Miki Sagara, Yukio Yamori
Summary: Plant polyphenols, such as genistein and epigallocatechin-3-gallate, have been studied for their potential health benefits, particularly in relation to cancer and cardiovascular diseases. However, there are conflicting findings regarding the effects of these flavonoids, with some studies showing positive effects while others do not. Factors such as recall bias and the measurement of metabolites in urine samples can contribute to these inconsistencies. Further clinical studies considering different confounding factors are needed to provide evidence for the actual impact of dietary flavonoids on human diseases. One possible mechanism involves the regulation of reactive oxygen species and 5'-adenosine monophosphate-activated protein kinase by flavonoids.
Review
Biochemistry & Molecular Biology
Sumio Hayakawa, Tomokazu Ohishi, Yumiko Oishi, Mamoru Isemura, Noriyuki Miyoshi
Summary: Polyphenols have anticancer effects by acting as antioxidants and modulating cell signaling pathways, with ncRNA modulation enhancing these effects.
Article
Nutrition & Dietetics
Kazuaki Iguchi, Koji Nagashima, Jun Mochizuki, Hiroyuki Yamamoto, Keiko Unno, Noriyuki Miyoshi
Summary: Several studies have shown that the consumption of certain mushroom species can affect testosterone production in animals. Enokitake mushrooms and vegetables containing adenosine might influence testosterone production. This study investigated the effects of enokitake and adenosine on testosterone production in testicular cells in mice models. The results suggest that intake of adenosine-containing mushrooms and vegetables, particularly enokitake, may be beneficial for increasing testicular testosterone production and combating aging and fatigue.
Article
Biochemistry & Molecular Biology
Yasukiyo Yoshioka, Shiori Oishi, Keita Onoda, Katsumi Shibata, Noriyuki Miyoshi
Summary: The study found that diosgenin is an effective functional food for preventing glucocorticoid-induced skeletal muscle atrophy. It acts by inhibiting protein expression, improving phosphorylation levels, and blocking nuclear translocation.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Review
Biochemistry & Molecular Biology
Takuji Suzuki, Tomokazu Ohishi, Hiroki Tanabe, Noriyuki Miyoshi, Yoriyuki Nakamura
Summary: Matrix metalloproteinases (MMPs) are zinc-dependent metalloproteinases that are involved in various diseases. Polyphenols have been demonstrated to have beneficial effects on these diseases by suppressing MMP gene expression and enzyme activity, as well as inhibiting reactive oxygen species and directly binding to MMPs. Molecular docking analyses can provide a structural basis for the development of polyphenol-based drugs with anti-inflammatory properties.
Article
Biochemistry & Molecular Biology
Shunsuke Yamamoto, Shigeki Kato, Nanami Senoo, Noriyuki Miyoshi, Akihito Morita, Shinji Miura
Summary: The study found significant differences in PC profiles between different meat animal species and cut sites, with pork meat having high amounts of PCs containing ether bonds and beef and lamb meats containing PCs with an odd number of carbon atoms. Additionally, PC profiles varied based on muscle location in chicken and pork, suggesting that skeletal muscle PC profiles can be indicators of animal species and muscle location.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
