Article
Cardiac & Cardiovascular Systems
My-Anh Nguyen, Huy-Dung Hoang, Adil Rasheed, Anne-Claire Duchez, Hailey Wyatt, Mary Lynn Cottee, Tyson E. Graber, Leah Susser, Sabrina Robichaud, Ibrahim Berber, Michele Geoffrion, Mireille Ouimet, Hilal Kazan, Lars Maegdefessel, Erin E. Mulvihill, Tommy Alain, Katey J. Rayner
Summary: miR-223 acts as a key regulator in macrophages, protecting against atherosclerosis by modulating cholesterol metabolism and inflammation pathways.
CIRCULATION RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Yuan Zhang, Shengqian Dou, Xia Qi, Zhenzhen Zhang, Yujie Qiao, Yani Wang, Jin Xie, Hui Jiang, Bin Zhang, Qingjun Zhou, Qun Wang, Lixin Xie
Summary: This study identified the regulatory role of miR-223-5p in diabetic corneal epithelial and nerve regeneration, mediating inflammatory processes and cell proliferation signaling. Silencing miR-223-5p may contribute to the development of potential therapeutic strategies for diabetic keratopathy.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Cell Biology
Ruifan Wu, Shengchun Feng, Fan Li, Gang Shu, Lina Wang, Ping Gao, Xiaotong Zhu, Canjun Zhu, Songbo Wang, Qingyan Jiang
Summary: Obesity is a global health problem associated with metabolic diseases. This study investigates the role of YBX1 in adipogenesis and adipose tissue expansion. The researchers found that YBX1 deficiency inhibited adipocyte differentiation and that YBX1 promotes adipogenesis through ULK1- and ULK2-mediated autophagy. The findings provide insights into the molecular mechanisms and functional importance of YBX1 in regulating autophagy and adipogenesis, highlighting its potential as a therapeutic target for obesity and metabolic diseases.
CELL DEATH & DISEASE
(2023)
Article
Biology
Vijay K. Samineni, Jose G. Grajales-Reyes, Gary E. Grajales-Reyes, Eric Tycksen, Bryan A. Copits, Christian Pedersen, Edem S. Ankudey, Julian N. Sackey, Sienna B. Sewell, Michael R. Bruchas, Robert W. Gereau
Summary: Studies have shown that chloroquine-activated neurons in the CeA play a crucial role in regulating itch sensation and associated aversion, as well as in itch-related comorbidities such as anxiety-like behaviors. These neurons modulate itch through differentially expressed genes and signaling pathways, projecting to key brain regions involved in itch modulation.
Review
Immunology
Julian J. Freen-van Heeren
Summary: T cells are crucial for eliminating infected and malignantly transformed cells as part of the adaptive immune system. Inflammatory cytokine production is tightly regulated on multiple levels, with post-transcriptional regulation playing a significant role in fine-tuning T-cell effector function, especially in anti-tumour responses. Exhausted or dysfunctional T cells in cancer patients possess mRNA for inflammatory mediators but fail to produce effector molecules, indicating a potential role of post-transcriptional events in hindering tumour-infiltrating lymphocyte effector function.
Article
Biochemistry & Molecular Biology
Michela Casanova, Francesca Pontis, Patrizia Ghidotti, Ilaria Petraroia, Lara Veronica Venturini, Luca Bergamaschi, Stefano Chiaravalli, Loris De Cecco, Maura Massimino, Gabriella Sozzi, Andrea Ferrari, Orazio Fortunato, Patrizia Gasparini
Summary: This study found that miR-223 plays a crucial role in AYA-RMS development and aggressiveness, as it impairs tumor proliferation and invasiveness, induces apoptosis, and causes cell cycle arrest. IGF1R is identified as a direct target of miR-223 in AYA-RMS cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Melodie Migault, Sunil Sapkota, Cameron P. Bracken
Summary: This review discusses the large number of regulators involved in epithelial-mesenchymal transition (EMT) and the implications for understanding EMT and the genetic machinery controlling complex biological processes.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Immunology
Xinyi Yang, Xiaying Zhao, Yuqi Zhu, Jingna Xun, Qin Wen, Hanyu Pan, Jinlong Yang, Jing Wang, Zhimin Liang, Xiaoting Shen, Yue Liang, Qinru Lin, Huitong Liang, Min Li, Jun Chen, Shibo Jiang, Jianqing Xu, Hongzhou Lu, Huanzhang Zhu
Summary: Using a genome-wide CRISPR-Cas9 activation library screening, the study identified FBXO34 and hnRNP U as new host factors related to HIV-1 latent maintenance. Overexpression of FBXO34 or knockout of hnRNP U can activate latent HIV-1 in multiple cell lines. The study provides mechanistic insights into the critical role of ubiquitination and hnRNP U in HIV-1 latency.
EMERGING MICROBES & INFECTIONS
(2022)
Review
Biochemistry & Molecular Biology
Sylvain Durand, Maude Guillier
Summary: In oxygen-limited environments, aerobic bacteria can shift to anaerobic respiration using nitrate as an alternative electron acceptor. Recent studies have shown that small regulatory RNAs play an important role in controlling the expression of genes related to nitrate respiration. These findings suggest that sRNAs are central in regulating metabolic pathways.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Medicine, General & Internal
Mingyu Shi, Qianying Lu, Yanmei Zhao, Ziling Ding, Sifan Yu, Junfeng Li, Mengjun Ji, Haojun Fan, Shike Hou
Summary: Small noncoding RNAs, microRNAs (miRNAs), play a crucial role in regulating various biological processes. The miRNA miR-223, which is expressed in myeloid lineage cells, is conserved throughout evolution and involved in the regulation of monocyte-macrophage differentiation, proinflammatory responses, and neutrophil recruitment. This review outlines the regulatory role of miR-223 in different cell types and summarizes its potential role in pulmonary inflammatory diseases such as COPD, ALI, COVID-19, providing insights into the molecular regulatory networks in these diseases.
FRONTIERS IN MEDICINE
(2023)
Review
Cell Biology
Brittany J. Bisnett, Brett M. Condon, Caitlin H. Lamb, George R. Georgiou, Michael Boyce
Summary: COPII, an ancient and essential pathway in eukaryotes, mediates forward trafficking of protein and lipid cargoes from the endoplasmic reticulum. Despite its broad significance, major aspects of COPII trafficking remain incompletely understood. Recent studies have highlighted the role of post-transcriptional mechanisms in regulating the COPII pathway.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Rosario Avolio, Marta Ingle-Ferrnandiz, Annagiulia Ciocia, Olga Coll, Sarah Bonnin, Tanit Guitart, Anna Ribo, Fatima Gebauer
Summary: This study reveals that the RNA-binding protein CSDE1 enables oncogene-induced senescence (OIS) in mouse keratinocytes. They identified two molecular mechanisms by which CSDE1 promotes OIS and showed that depletion of YBX1 can rescue senescence and uncouple proliferation arrest from the senescence-associated secretory phenotype (SASP).
Article
Biochemistry & Molecular Biology
Pasi K. Korhonen, Tao Wang, Neil D. Young, Gangi R. Samarawickrama, Deepani D. Fernando, Guangxu Ma, Robin B. Gasser, Katja Fischer
Summary: This study revealed significant negative and positive correlations between miRNAs and gene transcription levels between different developmental stages of Sarcoptes scabiei, which may be associated with processes such as metabolism and genetic information processing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemical Research Methods
Mattia Furlan, Stefano de Pretis, Mattia Pelizzola
Summary: Despite RNA abundance being commonly assumed as a proxy for transcriptional activity, it is now possible to calculate how synthesis, processing, and degradation rates collectively determine the abundance of each gene's RNA. Different transcriptional outputs can correspond to different combinations of kinetic rates, indicating the existence of markedly different modes of gene expression regulation and their profound effects on gene self-expression regulation.
BRIEFINGS IN BIOINFORMATICS
(2021)
Review
Biochemistry & Molecular Biology
Peng Jiao, Xing-Ping Wang, Zhuo-Ma Luoreng, Jian Yang, Li Jia, Yun Ma, Da-Wei Wei
Summary: miR-223 is an important miRNA that plays a critical role in various biological processes such as cell differentiation and immune modulation. It is regulated by different transcription factors and mediates its biological functions through changes in cellular or tissue expression. miR-223 is involved in regulating the differentiation of granulocytes, dendritic cells, as well as inflammation in diseases like lung inflammation and rheumatoid arthritis.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Cell Biology
Wendy Dankers, Md Abul Hasnat, Vanesa Swann, Arwaf Alharbi, Jacinta P. W. Lee, Megan A. Cristofaro, Michael P. Gantier, Sarah A. Jones, Eric F. Morand, Jacqueline K. Flynn, James Harris
IMMUNOLOGY AND CELL BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Arwaf S. Alharbi, Aurelie J. Garcin, Kim A. Lennox, Solene Pradeloux, Christophe Wong, Sarah Straub, Roxane Valentin, Genevieve Pepin, Hong-Mei Li, Marcel F. Nold, Claudia A. Nold-Petry, Mark A. Behlke, Michael P. Gantier
NUCLEIC ACIDS RESEARCH
(2020)
Editorial Material
Respiratory System
Michael P. Gantier
Letter
Respiratory System
Michael P. Gantier
Article
Biochemistry & Molecular Biology
Roxane Valentin, Christophe Wong, Arwaf S. Alharbi, Solene Pradeloux, Makala P. Morros, Kim A. Lennox, Julia Ellyard, Aurelie J. Garcin, Tomalika R. Ullah, Gina D. Kusuma, Genevieve Pepin, Hong-Mei Li, Jaclyn S. Pearson, Jonathan Ferrand, Rebecca Lim, Rakesh N. Veedu, Eric F. Morand, Carola G. Vinuesa, Mark A. Behlke, Michael P. Gantier
Summary: The study demonstrates that 2'OMe ASOs exhibit sequence-dependent inhibitory effects on RNA sensor TLR7, DNA sensors cGAS and TLR9. Different ASO sequences have varying potency in inhibiting these immune sensors, with only a few shown to inhibit both cGAS and TLR9 pathways. This complex pattern of immunomodulation confounds the interpretation of in vivo functions of ASOs.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell & Tissue Engineering
Mihiri Goonetilleke, Nathan Kuk, Jeanne Correia, Alex Hodge, Gregory Moore, Michael P. Gantier, George Yeoh, William Sievert, Rebecca Lim
Summary: The study found that hAEC administration attenuated the expansion of liver progenitor cells, hepatic inflammation, and oxidative stress in an experimental model of NASH.
STEM CELL RESEARCH & THERAPY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Michael P. Gantier
Summary: cGAS plays a crucial role in innate immunity by detecting pathogenic DNA molecules and monitoring cellular health. A recent study has revealed a novel mechanism involving the formation of primary condensates of cGAS and G3BP1 to activate DNA sensing.
Article
Biochemistry & Molecular Biology
Mark Ziemann, Sze Chern Lim, Yilin Kang, Sona Samuel, Isabel Lopez Sanchez, Michael Gantier, Diana Stojanovski, Matthew McKenzie
Summary: MiR-101-3p acts as a tumor suppressor that regulates cancer proliferation and apoptotic signaling. It downregulates multiple mitochondrial processes, disrupts mitochondrial function, and modulates mitochondrial metabolism by regulating mtDNA transcription and complex II biogenesis.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Sumaiah S. Al-Asmari, Aleksandra Rajapakse, Tomalika R. Ullah, Genevieve Pepin, Laura V. Croft, Michael P. Gantier
Summary: Activation of the STING pathway in cancer cells upon genotoxic treatment leads to the production of interferon-beta and anti-tumoral effects. However, it is also associated with the expression of pro-tumorigenic genes. In this study, it was found that genotoxic DNA damage rapidly increased the expression of IL-6 in STING-expressing cancer cells, independent of type-I interferon responses. The production of IL-6 was driven through both canonical and non-canonical STING signaling pathways. Inhibition of ERK1/2 could inhibit IL-6 production induced by DNA damage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Grant J. Brown, Pablo F. Canete, Hao Wang, Arti Medhavy, Josiah Bones, Jonathan A. Roco, Yuke He, Yuting Qin, Jean Cappello, Julia I. Ellyard, Katharine Bassett, Qian Shen, Gaetan Burgio, Yaoyuan Zhang, Cynthia Turnbull, Xiangpeng Meng, Phil Wu, Eun Cho, Lisa A. Miosge, T. Daniel Andrews, Matt A. Field, Denis Tvorogov, Angel F. Lopez, Jeffrey J. Babon, Cristina Aparicio Lopez, Africa Gonzalez-Murillo, Daniel Clemente Garulo, Virginia Pascual, Tess Levy, Eric J. Mallack, Daniel G. Calame, Timothy Lotze, James R. Lupski, Huihua Ding, Tomalika R. Ullah, Giles D. Walters, Mark E. Koina, Matthew C. Cook, Nan Shen, Carmen de Lucas Collantes, Ben Corry, Michael P. Gantier, Vicki Athanasopoulos, Carola G. Vinuesa
Summary: Enhanced Toll-like receptor 7 (TLR7) signaling has been associated with human systemic autoimmune disease, but evidence of TLR7 gene variants causing lupus is lacking. In this study, researchers identified a newly described TLR7(Y264H) variant that increased sensing of guanosine and 2',3'-cGMP and was sufficient to cause lupus in mice. Enhanced TLR7 signaling was shown to drive aberrant B cell survival and the accumulation of specific B cell subsets, while deficiency of the downstream adapter protein MyD88 rescued autoimmunity and all phenotypes. The study highlights the importance of TLR7 and guanosine-containing self-ligands in the pathogenesis of lupus and suggests potential therapeutic targets.
Article
Multidisciplinary Sciences
Annemarie Steiner, Katja Hrovat-Schaale, Ignazia Prigione, Chien-Hsiung Yu, Pawat Laohamonthonkul, Cassandra R. Harapas, Ronnie Ren Jie Low, Dominic De Nardo, Laura F. Dagley, Michael J. Mlodzianoski, Kelly L. Rogers, Thomas Zillinger, Gunther Hartmann, Michael P. Gantier, Marco Gattorno, Matthias Geyer, Stefano Volpi, Sophia Davidson, Seth L. Masters
Summary: Deficiency in COPI complex I may lead to aberrant activation of the STING pathway and inflammatory diseases, with cGAS possibly being the upstream immune sensor driving this process. Targeted inhibition of the cGAS/STING pathway can alleviate inflammation in diseases such as COPA syndrome.
NATURE COMMUNICATIONS
(2022)
Article
Microbiology
Tomalika R. Ullah, Katherine R. Balka, Rebecca L. Ambrose, Genevieve Pepin, Matthew C. J. Wilce, Jacqueline A. Wilce, Belinda J. Thomas, Dominic De Nardo, Bryan R. G. Williams, Michael P. Gantier
Summary: Cytoplasmic detection of DNA is an essential component of antiviral responses. Genistein, a compound found naturally in soy-based foods, inhibits cGAS-STING antiviral signaling, weakening the antiviral activity.
Article
Multidisciplinary Sciences
Tomalika R. Ullah, Matt D. Johansen, Katherine R. Balka, Rebecca L. Ambrose, Linden J. Gearing, James Roest, Julian P. Vivian, Sunil Sapkota, W. Samantha N. Jayasekara, Daniel S. Wenholz, Vina R. Aldilla, Jun Zeng, Stefan Miemczyk, Duc H. Nguyen, Nicole G. Hansbro, Rajan Venkatraman, Jung Hee Kang, Ee Shan Pang, Belinda J. Thomas, Arwaf S. Alharbi, Refaya Rezwan, Meredith O'Keeffe, William A. Donald, Julia I. Ellyard, Wilson Wong, Naresh Kumar, Benjamin T. Kile, Carola G. Vinuesa, Graham E. Kelly, Olivier F. Laczka, Philip M. Hansbro, Dominic De Nardo, Michael P. Gantier
Summary: The small molecule idronoxil inhibits the signalling of TBK1/IKK epsilon, a key signalling component involved in antiviral activities and pro-inflammatory responses. This finding highlights the critical role of TBK1/IKK epsilon signalling in SARS-CoV-2 hyper-inflammation.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Marion C. Bichet, Jack Adderley, Laura Avellaneda-Franco, Isabelle Magnin-Bougma, Natasha Torriero-Smith, Linden J. Gearing, Celine Deffrasnes, Cassandra David, Genevieve Pepin, Michael P. Gantier, Ruby C. Y. Lin, Ruzeen Patwa, Gregory W. Moseley, Christian Doerig, Jeremy J. Barr
Summary: It has been found that bacteriophage T4 does not activate DNA-mediated inflammatory pathways, but triggers protein phosphorylation cascades that promote cellular growth and survival. This finding suggests that mammalian cells internalize bacteriophages as a resource to enhance cellular growth and metabolism.
Article
Biochemistry & Molecular Biology
Pacome B. Prompsy, John Toubia, Linden J. Gearing, Randle L. Knight, Samuel C. Forster, Cameron P. Bracken, Michael P. Gantier
Summary: This study investigated the relationship between miRNA-regulons and transcription factors, finding that approximately 50% of miRNA targets were enriched in at least one common transcription factor. By leveraging miRNA-TF co-regulatory networks to identify pathways under miRNA control, filtering predicted miRNA-target interactions significantly enriched the proportion of predicted true interactions. This in-silico pipeline is the first of its kind to facilitate the identification of miRNA-regulons and aid in understanding miRNA function.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
