Article
Cell & Tissue Engineering
Zhuangzhuang Yang, Miao Yu, Xuechun Li, Yuanyuan Tu, Chunyan Wang, Wei Lei, Min Song, Yong Wang, Ying Huang, Fengyue Ding, Kaili Hao, Xinglong Han, Xuan Ni, Lina Qu, Zhenya Shen, Shijun Hu
Summary: This study demonstrates that retinoic acid inhibits angiogenesis and suppresses glycolysis in human embryonic stem cell-derived endothelial cells. The inhibitory effect is mediated by upregulation of fructose-1,6-bisphosphatase 1 (FBP1), a key enzyme in gluconeogenesis, and is dependent on the retinoic acid receptor (RAR). These findings suggest that FBP1 may serve as a novel therapeutic target for pathological angiogenesis.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Biochemistry & Molecular Biology
Geoffrey Brown
Summary: All-trans retinoic acid plays a role in embryogenesis and also acts as a teratogen. The deregulation of cytosolic aldehyde dehydrogenases in various human cancers affects the synthesis of all-trans retinoic acid. Inhibiting these enzymes reduces cancer cell proliferation, induces apoptosis, and enhances chemotherapeutic sensitivity. RAR gamma is an oncogene in several types of cancer, and inhibiting its action leads to cancer cell death. Targeting the retinoic acid pathway shows promise in eliminating cancer stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Kohei Yamakawa, Michiyo Koyanagi-Aoi, Akihito Machinaga, Nobuyuki Kakiuchi, Tomonori Hirano, Yuzo Kodama, Takashi Aoi
Summary: This study investigated the expression of retinoic acid receptor α (RARα) in pancreatic ductal adenocarcinoma (PDAC), and found that high RARα expression correlated with poor prognosis in PDAC patients. Further experiments showed that blockade of RARα signaling suppressed PDAC cell proliferation by inducing cell cycle arrest and had a synergistic effect with gemcitabine. These findings suggest that RARα signaling could be a potential therapeutic target for PDAC.
CANCER CELL INTERNATIONAL
(2023)
Article
Physiology
Kaidi Jin, Qianqian Jin, Zhongqiang Cai, Baoyu Huang, Lei Wei, Meiwei Zhang, Wen Guo, Yaqiong Liu, Xiaotong Wang
Summary: Recent studies reveal that retinoic acid receptors have a higher expression level in mollusks, potentially playing a role in development and metabolism. After injection with retinoic acid, both receptors showed elevated expression levels, indicating a potential regulatory mechanism in mollusks. Additionally, the receptors can interact to form a heterodimer complex, suggesting a conserved function in signaling pathways.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Anusha Dravid, Brad Raos, Darren Svirskis, Simon J. O'Carroll
Summary: Neuronal models are crucial in understanding neuroscientific processes, and adapting them to a high-throughput format allows for simultaneous screening of multiple agents. Differentiating SH-SY5Y cells is necessary to obtain mature neurons, but the absence of a standardized protocol hinders their use in high-throughput assays.
SCIENTIFIC REPORTS
(2021)
Article
Gastroenterology & Hepatology
Vu L. Ngo, Zhenda Shi, Baoming Jiang, Andrew T. Gewirtz
Summary: Prevention of RV infection by gut-resident SFB is mediated by the generation of RA, which activates RAR signaling and enhances enterocyte migration to impede RV infection.
Article
Oncology
Siriwoot Butsri, Veerapol Kukongviriyapan, Laddawan Senggunprai, Sarinya Kongpetch, Auemduan Prawan
Summary: The study demonstrates that all-trans-retinoic acid (ATRA) exerts cytotoxic effects on cholangiocarcinoma cells by increasing intracellular reactive oxygen species (ROS) levels and modulating apoptotic pathways to induce cell apoptosis.
Article
Genetics & Heredity
Veronique Caron, Nicolas Chassaing, Nicola Ragge, Felix Boschann, Angelina My-Hoa Ngu, Elisabeth Meloche, Sarah Chor, Saquib A. Lakhani, Weizhen Ji, Laurie Steiner, Julien Marcadier, Philip R. Jansen, Laura A. van de Pol, Johanna M. van Hagen, Alvaro Serrano Russi, Gwenael Le Guyader, Magnus Nordenskjold, Ann Nordgren, Britt-Marie Anderlid, Julie Plaisancie, Corinna Stoltenburg, Denise Horn, Anne Drenckhahn, Fadi F. Hamdan, Mathilde Lefebvre, Tania Attie-Bitach, Peggy Forey, Vasily Smirnov, Francoise Ernould, Marie-Line Jacquemont, Sarah Grotto, Alberto Alcantud, Alicia Coret, Rosario Ferrer-Avargues, Siddharth Srivastava, Catherine Vincent-Delorme, Shelby Romoser, Nicole Safina, Dimah Saade, James R. Lupski, Daniel G. Calame, David Genevieve, Nicolas Chatron, Caroline Schluth-Bolard, Kenneth A. Myers, William B. Dobyns, Patrick Calvas, Caroline Salmon, Richard Holt, Frances Elmslie, Marc Allaire, Daniil M. Prigozhin, Andre Tremblay, Jacques L. Michaud
Summary: This study reports 25 individuals with pathogenic or likely pathogenic variants in the RARB gene, aiming to characterize their functional impact and the clinical spectrum of MCOPS12. The findings show that all tested RARB variants exhibit either gain-of-function or loss-of-function activity, leading to disrupted RARB function and variable clinical manifestations associated with MCOPS12.
GENETICS IN MEDICINE
(2023)
Review
Medicine, General & Internal
Yong Peng, Xiang Deng, Qiuming Zeng, Yandan Tang
Summary: This study provides a review and discussion on the role of CD4(+) and CD8(+) T cells in autoimmune diseases like multiple sclerosis. It also addresses the importance of the interaction between Tc17 cells and Th17 cells in these diseases.
CHINESE MEDICAL JOURNAL
(2022)
Review
Physiology
Anqun Chen, Yu Liu, Yu Lu, Kyung Lee, John Cijiang He
Summary: Retinoic acid (RA) has dual effects in kidney disease, providing renal protection while also potentially inducing podocyte injury and apoptosis, highlighting the need to target specific RA-mediated pathways for effective therapeutic treatments.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Qi-Wen Wang, Ding-Jia-Cheng Jia, Jia-Min He, Yong Sun, Yun Qian, Qi-Wei Ge, Ya-Dong Qi, Qing-Yi Wang, Ying-Ying Hu, Lan Wang, Yan-Fei Fang, Hui-Qin He, Man Luo, Li-Jun Feng, Jian-Min Si, Zhang-Fa Song, Liang-Jing Wang, Shu-Jie Chen
Summary: L. intestinalis has a protective effect against ulcerative colitis by modulating the interaction between Th17 cells and gut epithelial cells. It inhibits Th17 cell differentiation by reducing the production of C/EBPA-driven SAA1 and SAA2, thus attenuating colitis progression.
Article
Biology
Faizzan S. Ahmad, Yongcheng Jin, Alexander Grassam-Rowe, Yafei Zhou, Meng Yuan, Xuehui Fan, Rui Zhou, Razik Mu-u-min, Christopher O'Shea, Ayman M. Ibrahim, Wajiha Hyder, Yasmine Aguib, Magdi Yacoub, Davor Pavlovic, Yanmin Zhang, Xiaoqiu Tan, Ming Lei, Derek A. Terrar
Summary: We demonstrate a novel approach to generate atrial-like cardiomyocytes (CMs) from human-induced pluripotent stem cells (hiPSCs) through the treatment of Gremlin 2 and retinoic acid. The generated CMs exhibited similar morphology and characteristics to adult atrial myocytes (AMs), including expression of CM proteins and striated appearance. Single-cell RNA sequencing also revealed high expression of atrial-specific transcripts in the generated CMs. This method provides a valuable tool for studying atrial cell signaling pathways and atrial fibrillation (AF).
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Immunology
Claudia A. Rivera, Violaine Randrian, Wilfrid Richer, Yohan Gerber-Ferder, Maria-Graciela Delgado, Aleksandra S. Chikina, Annika Frede, Chiara Sorini, Mathieu Maurin, Hana Kammoun-Chaari, Sara M. Parigi, Christel Goudot, Mar Cabeza-Cabrerizo, Sylvain Baulande, Sonia Lameiras, Pierre Guermonprez, Caetano Reis e Sousa, Marc Lecuit, Helene D. Moreau, Julie Helft, Danijela Matic Vignjevic, Eduardo J. Villablanca, Ana-Maria Lennon-Dumenil
Summary: There are two pools of cDC2s in the small intestine, originating from common pre-DC precursors, and their phenotypes are influenced by food-derived retinoic acid.
Article
Cell Biology
Trey Polvadore, Malcolm Maden
Summary: This study reveals the impact of retinoic acid treatment on the control of positional information during axolotl limb regeneration. Through RNA sequencing, the genes interacting with RAR alpha were identified, shedding light on the mechanism of action for future research.
Article
Cell Biology
Fathima N. Cassim Bawa, Raja Gopoju, Yanyong Xu, Shuwei Hu, Yingdong Zhu, Shaoru Chen, Kavita Jadhav, Yanqiao Zhang
Summary: The study reveals the important role of macrophage retinoic acid receptor alpha (RARα) in regulating atherosclerosis. It protects against atherosclerosis by inducing cholesterol efflux and inhibiting inflammation.
Article
Chemistry, Organic
Daiki Kato, Takuya Shiraishi, Hiroyuki Kagechika, Tomoya Hirano
Summary: Coumarin serves as a core structure for photofunctional molecules, offering regulatory functionality for fluorescent sensors and photoremovable protecting groups. The study demonstrates that the 6-arylcoumarin moiety can provide a multi-functional regulatory property, showing OFF-ON-OFF change in response to different pH values.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Ayana Yoshihara, Haru Kawasaki, Hiroyuki Masuno, Koki Takada, Nobutaka Numoto, Nobutoshi Ito, Naoya Hirata, Yasunari Kanda, Michiyasu Ishizawa, Makoto Makishima, Hiroyuki Kagechika, Aya Tanatani
Summary: This study synthesized amide derivatives of lithocholic acid derivative 3 and found that N-cyanoamide 6 is more active than 1 and 3.
Article
Biochemistry & Molecular Biology
Tadaho Nakamura, Fumito Naganuma, Uta Kudomi, Sueji Roh, Kazuhiko Yanai, Takeo Yoshikawa
Summary: This study demonstrates the importance of oral histidine supplementation in the histaminergic nervous system and its positive effects on impaired working memory induced by sleep deprivation. Histidine intake replenishes brain histamine and enhances neuronal activity, leading to the recovery of memory function.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Medicinal
Tsuyoshi Oikawa, Shinya Fujii, Shuichi Mori, Hiroyuki Masuno, Emiko Kawachi, Hiroyuki Kagechika
Summary: A series of retinobenzoic acids were synthesized to investigate the structure-activity relationship of retinoids at the hydrophobic moiety. Compound 24c with a t-butyldimethylsilyl group showed potent retinoid activity, especially towards RARγ. This study provides insights for the development of new RARγ ligands.
Article
Neurosciences
Rina Otsuka, Fumito Naganuma, Tadaho Nakamura, Hideki Miwa, Rumi Nakayama-Naono, Takuro Matsuzawa, Yurika Komatsu, Yuki Sato, Yuna Takahashi, Haruna Tatsuoka-Kitano, Kazuhiko Yanai, Takeo Yoshikawa
Summary: This study demonstrates that astrocytic HNMT contributes to histamine clearance after histamine release in the cortex, and plays a role in the regulation of motor coordination, locomotor activity, and vigilance state.
Article
Oncology
Hiroyuki Suzuki, Tomokazu Ohishi, Teizo Asano, Tomohiro Tanaka, Masaki Saito, Takeo Yoshikawa, Takuya Mizuno, Manabu Kawada, Mika K. K. Kaneko, Yukinari Kato
Summary: The study demonstrates that the anti-HER2 monoclonal antibody H77Bf possesses high binding affinity and exerts antitumor activities against dHER2-positive canine cancers, suggesting it could be a valuable treatment regimen for canine cancers.
Article
Chemistry, Organic
Shuichi Mori, Nozomi Tsuemoto, Emiko Kawachi, Chiharu Takubo, Aya Tanatani, Hiroyuki Kagechika
Summary: In this study, novel retinoids containing a trans-SF4-alkynyl group as a structural element were designed and synthesized. The compounds showed antagonistic activity towards retinoid acid receptors (RARs) and altered the binding mode of RARα ligand-binding domain.
Article
Medicine, Research & Experimental
Sadayoshi Ito, Yasuyuki Okuda, Kotaro Sugimoto
Summary: This study examined factors associated with the antihypertensive effects of esaxerenone and the incidence of serum potassium elevation in patients with hypertension. Results showed that factors such as sex, weight, hypertension duration, prior antihypertensive treatment, plasma aldosterone concentration, urinary albumin-to-creatinine ratio, and smoking were associated with changes in blood pressure. Factors significantly associated with serum potassium elevation included baseline serum potassium, renal function, urinary albumin-to-creatinine ratio, blood pressure, and hypertension grade. The study concluded that esaxerenone is effective for patients with various backgrounds, but careful management is needed for those at risk of elevated serum potassium.
ADVANCES IN THERAPY
(2023)
Article
Medicine, Research & Experimental
Guanjie Li, Hiroyuki Suzuki, Tomokazu Ohishi, Teizo Asano, Tomohiro Tanaka, Miyuki Yanaka, Takuro Nakamura, Takeo Yoshikawa, Manabu Kawada, Mika K. K. Kaneko, Yukinari Kato
Summary: EpCAM is a type I transmembrane glycoprotein highly expressed on tumor cells, and it has been considered as a promising target for tumor diagnosis and therapy. In this study, a defucosylated version of an anti-EpCAM monoclonal antibody was generated and evaluated for its antitumor activity against EpCAM-positive cells. The results showed that the antibody exhibited potent cytotoxicity for EpCAM-overexpressing cells and suppressed tumor development in xenograft mouse models.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Nami Tateyama, Hiroyuki Suzuki, Tomokazu Ohishi, Teizo Asano, Tomohiro Tanaka, Takuya Mizuno, Takeo Yoshikawa, Manabu Kawada, Mika K. Kaneko, Yukinari Kato
Summary: The bispecific antibody E134Bf-H77scFv exhibited antitumor activities against dEGFR/dHER2-positive canine tumors, suggesting its potential as a valuable treatment regimen.
Article
Biochemistry & Molecular Biology
Jennifer Nhieu, Liming Milbauer, Thomas Lerdall, Fatimah Najjar, Chin-Wen Wei, Ryosuke Ishida, Yue Ma, Hiroyuki Kagechika, Li-Na Wei
Summary: All-trans-retinoic Acid (atRA), derived from Vitamin A, plays crucial roles in various biological processes. It can modulate gene expression through nuclear RA receptors (RARs) or regulate cytoplasmic kinase signaling via cellular retinoic acid binding protein 1 (CRABP1). This study focuses on identifying CRABP1-binding ligands without RAR activity for potential therapeutic applications. The researchers developed a P19-MN differentiation system to investigate the effects of CRABP1 ligands in motor neuron (MN) differentiation and discovered C32 and C4 as CRABP1 ligands that can modulate CaMKII activation and protect against excitotoxicity-triggered MN death. These findings suggest the potential of selective and CRABP1-binding ligands in mitigating MN degenerative diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Kimiko Tanaka, Fumi Takeda, Midori Kanda, Masatoshi Kawahata, Shinya Fujii, Kosuke Katagiri, Hyuma Masu, Hiroyuki Kagechika, Aya Tanatani
Summary: This study found that the crystal structure of aromatic N-monomethylsquaramide is in the (trans, cis) form, but in solution, it exhibits a solvent-dependent conformational equilibrium. Therefore, aromatic N-monomethylsquaramides could be used as components of environmentally responsive molecular switches.
JOURNAL OF ORGANIC CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jason P. Mansell, Aya Tanatani, Hiroyuki Kagechika
Summary: The combination of the new VDR agonist LCA-150 with LPA has synergistic effects on promoting osteoblast maturation, surpassing the efficacy of vitamin D.
Article
Immunology
Nami Tateyama, Teizo Asano, Hiroyuki Suzuki, Guanjie Li, Takeo Yoshikawa, Tomohiro Tanaka, Mika K. K. Kaneko, Yukinari Kato
Summary: CCR3 is a receptor for CC chemokines and is involved in various allergic diseases and cancers. In this study, the binding epitopes of specific monoclonal antibodies targeting CCR3 were investigated, revealing the N-terminal region of CCR3 as the key recognition site for these antibodies. This research provides a foundation for further drug development and studies of CCR3-related therapies.
Article
Immunology
Teizo Asano, Tomohiro Tanaka, Hiroyuki Suzuki, Guanjie Li, Tomokazu Ohishi, Manabu Kawada, Takeo Yoshikawa, Mika K. K. Kaneko, Yukinari Kato
Summary: In this study, an anti-EpCAM monoclonal antibody called EpMab-37-mG(2a)-f was found to have antitumor activities against breast and pancreatic cancers by inducing antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity. The administration of this antibody significantly suppressed the development of xenograft tumors.