Article
Chemistry, Multidisciplinary
Xue-Mei Zhou, Ying Zhuo, Ruo Yuan, Ya-Qin Chai
Summary: Here, giant DNA networks were created using two types of functionalized tetrahedral DNA nanostructures (f-TDNs) for sensitive detection and intracellular imaging of APE1, as well as gene therapy in tumor cells. These giant DNA networks significantly enhanced the fluorescence signal and achieved sensitive detection of APE1. The f-TDNs also exhibited enhanced targeting activity to tumor cells and allowed for selective imaging of intracellular APE1, while the siRNA carried by f-TDN1 could be accurately released for tumor cell apoptosis in the presence of APE1.
Article
Multidisciplinary Sciences
Yuriki Sakurai, Kyungmin Baeg, Andy Y. W. Lam, Keisuke Shoji, Yukihide Tomari, Hiro-oki Iwakawa
Summary: This study uncovers the molecular mechanisms underlying the production of secondary siRNAs in plants, and highlights the impact of the integrity of different small RNA binding sites on RDR6.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biotechnology & Applied Microbiology
Syed Saqib Ali Zaidi, Faria Fatima, Syed Aqib Ali Zaidi, Dezhong Zhou, Wuquan Deng, Shuai Liu
Summary: siRNA has the potential to silence specific genes, but it is limited by biological barriers. Strategies such as chemical modifications, encapsulation or formulation, and various delivery techniques have been developed to overcome these limitations and improve the bioavailability and therapeutic potential of siRNA.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Lisa N. Kasiewicz, Souvik Biswas, Aaron Beach, Huilan Ren, Chaitali Dutta, Anne Marie Mazzola, Ellen Rohde, Alexandra Chadwick, Christopher Cheng, Sara P. Garcia, Sowmya Iyer, Yuri Matsumoto, Amit V. Khera, Kiran Musunuru, Sekar Kathiresan, Padma Malyala, Kallanthottathil G. Rajeev, Andrew M. Bellinger
Summary: Lipid nanoparticles are effective in delivering therapeutic drugs through low-density lipoprotein receptor-mediated endocytosis. For patients with low low-density lipoprotein receptor activity, an alternative strategy is needed. By optimizing a GalNAc-Lipid nanoparticle design, independent delivery is achieved.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Organic
Alexander Pontarelli, Jiang Tian Liu, Hourieh Movasat, Sarah Menard, Jung Kwon Oh, Christopher J. Wilds
Summary: The synthesis and incorporation of a tosylated phosphoramidite linker containing a disulfide bond is described. This linker was efficiently incorporated into short DNA and RNA oligomers using automated solid phase synthesis, allowing for the expansion of strategies for bifunctional modification of synthetic oligonucleotides for conjugation applications.
Article
Chemistry, Multidisciplinary
Hong Yu, Yi Li, Ruirui Zhang, Mengchen Shen, Yuting Zhu, Qiang Zhang, Huiliang Liu, Dong Han, Xiaoli Shi, Jiao Zhang
Summary: In this study, a novel delivery system based on silica nanoparticles successfully enhanced the expression of miR-24 in cardiomyocytes, leading to the inhibition of apoptosis by suppressing the pro-apoptotic gene Bim. In vivo experiments further demonstrated that this delivery system also significantly improved ventricular remodeling and cardiac function post-coronary artery ligation.
NANOSCALE ADVANCES
(2021)
Article
Engineering, Electrical & Electronic
Zichong Chen, Chen Ma, Hongbo Chu, Zhuolin Deng, Songmin Chen, Gaosheng Li
Summary: In this paper, a proof of concept of the inflatable axial-mode helical antenna with retractability and releasability, made of PVC films and conductive tape, was optimized, fabricated, and tested. The inflatable antenna offers advantages of reduced volume, weight, and manufacturing cost, while maintaining a good impedance and axial ratio bandwidth. It has broad prospects in various fields such as emergency communication and military operations.
AEU-INTERNATIONAL JOURNAL OF ELECTRONICS AND COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Ivan V. Chernikov, Ulyana A. Ponomareva, Elena L. Chernolovskaya
Summary: The use of small interfering RNA (siRNA) in the clinic offers possibilities for treating previously incurable diseases, but the main challenge lies in delivering siRNA to target cells and organs. While siRNA delivery to liver cells has been solved through bioconjugation with N-acetylgalactosamine, it remains challenging for other organs. The structure and molecular weight of siRNA also play crucial roles in its delivery and activity, with smaller molecular weight siRNAs being more efficient at entering cells and larger ones having advantages at the organism level. This review aims to explore the relationship between siRNA structure and its biodistribution and activity to develop new strategies for improving siRNA performance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Plant Sciences
Junpeng Zhan, Blake C. Meyers
Summary: Plant cells accumulate small RNA molecules that regulate plant development, genome stability, and environmental responses. These small RNAs fall into three major classes-microRNAs, heterochromatic small interfering RNAs, and secondary small interfering RNAs-plus several other less well-characterized categories, and their biogenesis mechanisms, targets, modes of action, and functions have been extensively studied in Arabidopsis and other plant species, contributing to a better understanding of plant small RNA biology.
ANNUAL REVIEW OF PLANT BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Chi Zhu, Justin Y. Lee, Jia Z. Woo, Lei Xu, Xammy Nguyenla, Livia H. Yamashiro, Fei Ji, Scott B. Biering, Erik Van Dis, Federico Gonzalez, Douglas Fox, Eddie Wehri, Arjun Rustagi, Benjamin A. Pinsky, Julia Schaletzky, Catherine A. Blish, Charles Chiu, Eva Harris, Ruslan I. Sadreyev, Sarah Stanley, Sakari Kauppinen, Silvi Rouskin, Anders M. Naar
Summary: A novel therapeutic strategy using locked nucleic acid antisense oligonucleotides (LNA ASOs) has been developed to target SARS-CoV-2 RNA. It disrupts viral replication with high efficacy and has shown promising results in both in vitro and in vivo experiments, including COVID-19 mouse models. This approach could potentially complement current vaccination efforts and help reduce transmission and severity of COVID-19.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Dongyuan Wang, Yuan Tian, Yu Zhang, Xiaona Sun, Yuxuan Wu, Ruping Liu, Fang Zeng, Jingjing Du, Kuan Hu
Summary: This study identified a multifunctional peptide drug conjugate (PDC) as a safe and efficient carrier to deliver AS1411 to the nucleus of cancer cells. The PDC showed unexpected synergy with AS1411, enhancing its anticancer effect and reversing the stemness of cancer stem-like cells.
Review
Engineering, Biomedical
Zheng-Rong Lu, Victoria E. A. Laney, Ryan Hall, Nadia Ayat
Summary: Environment-responsive lipids are a novel type of siRNA delivery system that can respond to environmental changes during the delivery process. Lipid/siRNA nanoparticles have been developed for efficient delivery of therapeutic siRNA into cancer cells.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Chemistry, Analytical
Jun Yoshitake, Mayuko Azami, Haruka Sei, Daisuke Onoshima, Kumiko Takahashi, Akiyoshi Hirayama, Koji Uchida, Yoshinobu Baba, Takahiro Shibata
Summary: Extracellular vesicles are nanoscale lipid bilayer vesicles released by almost all cell types. They play an important role in cell-cell communication and have potential applications in disease diagnosis and drug delivery. In this study, a size-exclusion chromatography column device was developed for rapid and effective isolation of extracellular vesicles.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Manabu Yoshikawa, Yong-Woon Han, Hirofumi Fujii, Shu Aizawa, Tatsuya Nishino, Masayuki Ishikawa
Summary: In plants, siRNAs are amplified through cleavage by RISCs and play a crucial role in target repression. RDR6, SGS3, and SDE5 work together to ensure the specificity and efficiency of siRNA amplification and target inhibition in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Qinqin Cheng, Zhefu Dai, Goar Smbatyan, Alan L. Epstein, Heinz-Josef Lenz, Yong Zhang
Summary: Researchers designed genetically engineered exosomes with displayed antibodies and immunomodulatory proteins for immunotherapy of breast cancer. These exosomes can redirect and activate T cells to kill cancer cells and induce potent anti-tumor immune responses.
Letter
Allergy
Eric Tyrell Oliver, Patricia Meghan Sterba, Kelly Devine, Becky M. Vonakis, Sarbjit Singh Saini
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2016)
Article
Respiratory System
Jie Ma, Chuanxi Chen, Yongjun Liu, Mahendra Damarla, Becky M. Vonakis, Xiangdong Guan, Li Gao
JOURNAL OF THORACIC DISEASE
(2018)
Article
Hematology
Yufeng Zhou, Hui-Ying Tung, Ying-Ming Tsai, Shih-Chang Hsu, Hui-Wen Chang, Hirokazu Kawasaki, Hsiao-Chun Tseng, Beverly Plunkett, Peisong Gao, Chih-Hsing Hung, Becky M. Vonakis, Shau-Ku Huang
Article
Immunology
Sarbjit S. Saini, Miya Paterniti, Kavitha Vasagar, Scott P. Gibbons, Patricia M. Sterba, Becky M. Vonakis
CLINICAL IMMUNOLOGY
(2009)
Article
Medicine, Research & Experimental
Sarah K. Norton, Anthony Dellinger, Zhiguo Zhou, Robert Lenk, Darren MacFarland, Becky Vonakis, Daniel Conrad, Christopher L. Kepley
CTS-CLINICAL AND TRANSLATIONAL SCIENCE
(2010)
Review
Immunology
Becky M. Vonakis, Sarbjit S. Saini
CURRENT OPINION IN IMMUNOLOGY
(2008)
Article
Genetics & Heredity
Sunita Sharma, Amy Murphy, Judie Howrylak, Blanca Himes, Michael H. Cho, Jen-Hwa Chu, Gary M. Hunninghake, Anne Fuhlbrigge, Barbara Klanderman, John Ziniti, Jody Senter-Sylvia, Andy Liu, Stanley J. Szefler, Robert Strunk, Mario Castro, Nadia N. Hansel, Gregory B. Diette, Becky M. Vonakis, N. Franklin Adkinson, Vincent J. Carey, Benjamin A. Raby
GENETIC EPIDEMIOLOGY
(2011)
Article
Biochemistry & Molecular Biology
Amy Murphy, Jen-Hwa Chu, Mousheng Xu, Vincent J. Carey, Ross Lazarus, Andy Liu, Stanley J. Szefler, Robert Strunk, Karen DeMuth, Mario Castro, Nadia N. Hansel, Gregory B. Diette, Becky M. Vonakis, N. Franklin Adkinson, Barbara J. Klanderman, Jody Senter-Sylvia, John Ziniti, Christoph Lange, Tomi Pastinen, Benjamin A. Raby
HUMAN MOLECULAR GENETICS
(2010)
Letter
Allergy
Becky M. Vonakis, Sarhjit S. Saini
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2008)
Article
Allergy
Sunita Sharma, Xiaobo Zhou, Derek M. Thibault, Blanca E. Himes, Andy Liu, Stanley J. Szefler, Robert Strunk, Mario Castro, Nadia N. Hansel, Gregory B. Diette, Becky M. Vonakis, N. Franklin Adkinson, Lydiana Avila, Manuel Soto-Quiros, Albino Barraza-Villareal, Robert F. Lemanske, Julian Solway, Jerry Krishnan, Steven R. White, Chris Cheadle, Alan E. Berger, Jinshui Fan, Meher Preethi Boorgula, Dan Nicolae, Frank Gilliland, Kathleen Barnes, Stephanie J. London, Fernando Martinez, Carole Ober, Juan C. Celedon, Vincent J. Carey, Scott T. Weiss, Benjamin A. Raby
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2014)
Article
Immunology
David Larson, Marc P. Huebner, Marina N. Torrero, Christopher P. Morris, Amy Brankin, Brett E. Swierczewski, Stephen J. Davies, Becky M. Vonakis, Edward Mitre
JOURNAL OF IMMUNOLOGY
(2012)
Review
Biochemistry & Molecular Biology
Ronaldo P. Panganiban, Becky M. Vonakis, Faoud T. Ishmael, Cristiana Stellato
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
(2014)
Article
Immunology
Tiffany M. Link, Una Park, Becky M. Vonakis, Daniel M. Raben, Mark J. Soloski, Michael J. Caterina
Article
Respiratory System
Luca Ricciardi, Essica Dal Col, Paolo Casolari, Domenico Memoli, Valeria Conti, Alessandro Vatrella, Becky M. Vonakis, Alberto Papi, Gaetano Caramori, Cristiana Stellato
INTERNATIONAL JOURNAL OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE
(2018)
Article
Allergy
Eric Tyrell Oliver, Kris Chichester, Kelly Devine, Patricia Meghan Sterba, Craig Wegner, Becky Marie Vonakis, Sarbjit Singh Saini
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2019)