Journal
JOURNAL OF KOREAN MEDICAL SCIENCE
Volume 25, Issue 9, Pages 1284-1290Publisher
KOREAN ACAD MEDICAL SCIENCES
DOI: 10.3346/jkms.2010.25.9.1284
Keywords
Dendritic Cells; Prostatic Neoplasms; Cancer Vaccines; Immunotherapy
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Funding
- Asan Institute for Life Sciences, Seoul, Korea [2008-71]
- Korean Institute of Medicine
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Dendritic cells (DCs) are potent antigen-presenting cells. OK432 (Picibanil (R)) was introduced as a potent stimulator of DC maturation in combination with prostaglandin-E-2 and interferon-alpha. We compared the efficacy of a DC-prostate cancer vaccine using early-mature DCs stimulated with OK432, PGE2 and INF-alpha (OPA) with that of vaccines using other methods. On days 3 or 7 of DC culture, TNF-alpha (T), TNF-alpha and LPS (TL) or OPA were employed as maturation stimulators. DU145 cells subjected to heat stress were hybridized with mature DCs using polyethyleneglycol. T cells were sensitized by the hybrids, and their proliferative and cytokine secretion activities and cytotoxicity were measured. The yields of early-mature DCs were higher, compared to yields at the conventional maturation time (P<0.05). In the early maturation setting, the mean fusion ratios, calculated from the fraction of dual-positive cells, were 13.3%, 18.6%, and 39.9%, respectively (P=0.051) in the T only, TL, and OPA-treated groups. The function of cytotoxic T cells, which were sensitized with the hybrids containing DCs matured early with OPA, was superior to that using other methods. The antitumor effects of DC-DU145 hybrids generated with DCs subjected to early maturation with the OPA may be superior to that of the hybrids using conventional maturation methods.
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