Review
Immunology
Masaki Honda, Masashi Kadohisa, Daiki Yoshii, Yoshihiro Komohara, Taizo Hibi
Summary: To date, the precise roles of different immune cells in intestinal immunity remain unclear. Understanding their behavior and function in various gastrointestinal conditions, including inflammation, tumors, and tissue repair, is crucial for advancing gastrointestinal research. Intravital microscopy has provided new insights into intestine-specific events and immune responses. This review focuses on the emerging role of intravital imaging in gastrointestinal research and describes the observation of intestines and immune cells using this technique, as well as novel findings obtained.
INFLAMMATION AND REGENERATION
(2023)
Article
Oncology
Alexa R. Heaton, Peter R. Rehani, Anna Hoefges, Angelica F. Lopez, Amy K. Erbe, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic reprogramming plays a significant role in tumorigenesis and cancer progression. However, current techniques for measuring tumor and immune cell metabolism lack spatial context. In this study, a mouse model and an algorithm for single cell imaging of immune and cancer cells were developed. The results showed metabolic changes in tumor infiltrating immune cells compared to immune cells from spleens, as well as single cell metabolic heterogeneity in both immune and tumor cells.
FRONTIERS IN ONCOLOGY
(2023)
Review
Immunology
Arpan Sharma Neupane, Paul Kubes
Summary: Highly dynamic immune responses involve multiple immune cell types working together to remove pathogens or injured tissue and return to homeostasis. Intravital microscopy (IVM) has been used to study the distinct immune cell behaviors in individual organs, showing that these behaviors can also influence each other. This review discusses how IVM has been applied to study innate immune responses in various tissues during homeostasis, injury, and infection.
IMMUNOLOGICAL REVIEWS
(2022)
Review
Urology & Nephrology
Joana R. Martins, Dominik Haenni, Milica Bugarski, Marcello Polesel, Claus Schuh, Andrew M. Hall
Summary: The development of intravital imaging with multiphoton microscopy has revolutionized kidney research by allowing real-time visualization of dynamic cellular behavior and organelle function, providing insights into structure-function relationships in unprecedented detail. Advances in technology and image analysis have overcome practical challenges, opening up huge new possibilities for studying cellular mechanisms and disease states in the kidney.
KIDNEY INTERNATIONAL
(2021)
Review
Cell Biology
Nazli Alizadeh-Tabrizi, Stefan Hall, Christian Lehmann
Summary: Intravital microscopy is a unique imaging method that provides real-time insights into cellular functions without the need for tissue extraction. Despite specific challenges in lung imaging, advancements in surgical and optical techniques have enabled researchers to uncover mechanisms of leukocyte recruitment and function in models of pulmonary inflammation and infection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Endocrinology & Metabolism
Melia D. Matthews, Emily Cook, Nada Naguib, Ulrich B. Wiesner, Karl J. Lewis
Summary: Osteocytes, as the resident mechanosensory cells in bone, play a crucial role in skeletal homeostasis and adaptation. This study demonstrates the usage of ultra-small and bright C'Dots as a novel in vivo imaging tool for osteocytes, which improves intravital imaging capabilities. It reveals distinct sex differences in nanoparticle intracellular dynamics and clearance in osteocytes and provides the first evidence of osteocyte integrin endocytosis and recycling in vivo. The results offer novel insights in osteocyte biology and open up new lines of investigation previously unavailable in vivo.
Review
Immunology
Philippe Bousso, Capucine L. L. Grandjean
Summary: Modulating the immune system can be used to treat various diseases and also improve our understanding of immune responses. This review discusses how imaging techniques can help clarify the mechanism of action of immunomodulatory strategies and guide the development of new drugs.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Article
Immunology
Laurence S. C. Lok, Jennifer A. Walker, Helen E. Jolin, Seth T. Scanlon, Masaru Ishii, Padraic G. Fallon, Andrew N. J. McKenzie, Menna R. Clatworthy
Summary: ILC2s are crucial early effectors of mucosal type 2 immunity, producing IL-13 in lymphoid organs. In vivo imaging showed that ILC2s increase in size and movement after helminth infection, with increased interactions with T cells in the intestinal mucosa.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Juhee Pae, Johanne T. Jacobsen, Gabriel D. Victora
Summary: Germinal centers serve as the site for antibody affinity maturation, and multiphoton laser scanning microscopy has allowed insight into the dynamic biological processes within GCs. This technical advancement has greatly contributed to our understanding of GC biology.
IMMUNOLOGICAL REVIEWS
(2022)
Review
Immunology
Xuwen Peng, Yuke Wang, Jie Zhang, Zhihong Zhang, Shuhong Qi
Summary: Cancer immunotherapy has promising potential and understanding the interactions between immune cells and tumor cells in the tumor microenvironment is crucial. Intravital imaging has emerged as a powerful tool for visualizing dynamic processes and functions of immune cells, aiding in the development of more effective cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Cell Biology
Michael Mihlan, Shima Safaiyan, Manuel Stecher, Neil Paterson, Tim Laemmermann
Summary: Successful immune responses rely on the coordinated migration, interactions, and functioning of immune cells in lymphoid and parenchymal tissues. Real-time intravital microscopy has provided insights into the dynamic behavior of immune cells in living tissues, revealing unexpected biological features that were not observed in experiments outside of a living organism.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Johannes Rebling, Maya Ben-Yehuda Greenwald, Mateusz Wietecha, Sabine Werner, Daniel Razansky
Summary: A large-scale label-free optoacoustic microscopy approach has been developed for rapid and non-invasive imaging of tissue regeneration, providing new insights into angiogenesis and enabling automatic analysis of key features in wound angiogenesis. This approach is a versatile tool for preclinical research in tissue engineering and regenerative medicine, allowing for high-throughput and quantitative studies of vascular remodeling and response to pharmacological interventions in vivo.
Article
Biology
Kevin Schilling, Yuankn Zhai, Zhuang Zhou, Bin Zhou, Edward Brown, Xinping Zhang
Summary: We examined the formation and specification of blood vessels at the osteogenic and angiogenic interface in murine cranial bone defect repair using advanced optical techniques. Our study revealed dynamic changes in vessel types and their coupling with osteoblast expansion and bone tissue deposition/remodeling during repair. We also found heterogeneous oxygen distributions in capillary vessels at the healing site, with endothelial cells coupling to osteoblasts preferentially using glycolysis while osteoblasts rely more on OxPhos and potentially consume more oxygen at the repair site.
Article
Nanoscience & Nanotechnology
Ericka Chorniak, Yang Liu, Ren Odion, Wiguins Etienne, Aidan Canning, Smita K. Nair, Paolo Maccarini, Gregory M. Palmer, Brant A. Inman, Tuan Vo-Dinh
Summary: Bladder cancer is a common cancer with a high proportion of late stage diagnoses. This study developed a novel treatment for bladder cancer using a combination of gold nanostar-mediated photothermal therapy and checkpoint inhibitor immunotherapy. The results showed that this combined therapy was more effective than individual treatments. The study also used intravital optical imaging to investigate immune responses and immune cell accumulation in distant tumors following the treatment. The findings suggest that intravital optical imaging is an efficient tool for studying the mechanisms of photoimmunotherapy.
Article
Biotechnology & Applied Microbiology
Mattia Sarti, Maria Parlani, Luis Diaz-Gomez, Antonios G. Mikos, Pietro Cerveri, Stefano Casarin, Eleonora Dondossola
Summary: This study developed an integrated computational pipeline based on an innovative variant of the U-Net neural network to segment and quantify cellular and extracellular structures of interest. The software shows promise for automatically detecting the elements of the FBR, unraveling the complexity of this pathophysiological process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Dermatology
Annelie H. Musters, Conor Broderick, David Prieto-Merino, Andrea Chiricozzi, Giovanni Damiani, Ketty Peris, Sandipan Dhar, Abhishek De, Esther Freeman, Bernd W. M. Arents, Tim Burton, Angela Leigh-Ann L. Bosma, Ching-Chi Chi, Godfrey Fletcher, Aaron M. Drucker, Kenji Kabashima, Emilie F. de Monchy, Maitreyee Panda, Dmitri Robert Wall, Christian Vestergaard, Emmanuel Mahe, Laura Bonzano, Leila Kattach, Maddalena Napolitano, Maria Fernanda Ordonez-Rubiano, Eva Haufe, Cataldo Patruno, Alan D. Irvine, Phyllis Spuls, Carsten Flohr
Summary: Limited data suggest that patients with atopic dermatitis (AD) receiving systemic immunomodulatory treatments have a lower risk of severe COVID-19 complications. Monotherapy with dupilumab is associated with lower hospitalization rates compared to other therapies. However, combination systemic treatments, particularly those including systemic corticosteroids, have the highest risk of severe COVID-19.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Letter
Dermatology
Toshiaki Kogame, Satoru Yonekura, Paola Lovato, Masahiro Hirata, Riko Takimoto-ito, Tomoya Takegami, Takayoshi Komatsu-Fujii, Naotomo Kambe, Takashi Nomura, Mads A. Ropke, Kenji Kabashima
Summary: Despite the limited sample size and low frequency of skin ILCs in this study, the data suggest that ICOS+ ILCs express IL-13Ra1 and that the density of ICOS+ ILCs decreases after four weeks of tralokinumab treatment.
BRITISH JOURNAL OF DERMATOLOGY
(2023)
Article
Dermatology
Yuki Honda Keith, Atsushi Otsuka, Toshiaki Kogame, Hiroyuki Ito, Shunya Usui, Masakazu Fujimoto, Keita Jinnouchi, Masahiro Hirata, Kazumitsu Sugiura, Kenji Kabashima
JOURNAL OF DERMATOLOGY
(2023)
Letter
Dermatology
Marina Ueda, Toshiaki Kogame, Gyohei Egawa, Naotomo Kambe, Kenji Kabashima
JOURNAL OF DERMATOLOGY
(2023)
Article
Dermatology
Hidehisa Saeki, Masashi Akiyama, Masatoshi Abe, Atsuyuki Igarashi, Shinichi Imafuku, Yukihiro Ohya, Norito Katoh, Hideto Kameda, Kenji Kabashima, Yuichiro Tsunemi, Michihiro Hide, Mamitaro Ohtsuki
Summary: This guidance provides information on the use of oral JAK inhibitors for the treatment of atopic dermatitis. It explains the role of oral JAK inhibitors in inhibiting the signal transduction of cytokines involved in the pathogenesis of atopic dermatitis. It also highlights the authorized oral JAK inhibitors for atopic dermatitis in Japan and emphasizes the importance of considering disease factors, treatment factors, and patient backgrounds in the selection of treatment options.
JOURNAL OF DERMATOLOGY
(2023)
Review
Dermatology
R. Bissonnette, L. F. Eichenfield, E. Simpson, D. Thaci, K. Kabashima, J. P. Thyssen, E. Guttman-Yassky, F. P. Nunes, M. Gamalo, F. Ahmad, M. Kuligowski, K. Sun, C. Pipper, A. W. Christensen, P. D'Angelo, M. Milutinovic, A. Guettner, J. I. Silverberg
Summary: Despite the lack of guidelines for analyzing clinical trial data in atopic dermatitis (AD), using the estimand framework can help standardize the analysis and incorporate intercurrent events. Intercurrent events, such as rescue therapy and sleep deprivation, frequently occur in AD trials and their inconsistent handling limits result interpretation. Applying the estimand framework, as guided by the International Council for Harmonization of Technical Requirements for Pharmaceuticals for Human Use (ICH), can improve AD trial design and analysis by providing more reflective and informative results for clinicians to make treatment selection.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Medicine, General & Internal
Emma Guttman-Yassky, Eric L. Simpson, Kristian Reich, Kenji Kabashima, Ken Igawa, Tetsuya Suzuki, Hirotaka Mano, Takeshi Matsui, Ehsanollah Esfandiari, Masutaka Furue
Summary: This study evaluated the efficacy and safety of the anti-OX40 antibody rocatinlimab in patients with moderate-to-severe atopic dermatitis. The results showed significant reductions in EASI score at week 16 in patients receiving rocatinlimab compared to placebo. The treatment led to progressive improvements in atopic dermatitis, which were maintained in most patients after treatment discontinuation, and it was well tolerated.
Article
Allergy
Riko Takimoto-Ito, Naotomo Kambe, Toshiaki Kogame, Takashi Nomura, Kazushi Izawa, Tomoyasu Jo, Yasuhiro Kazuma, Hajime Yoshifuji, Yuya Tabuchi, Hiroyasu Abe, Mayuko Yamamoto, Kimiko Nakajima, Ozumi Tomita, Yosuke Yagi, Kazumoto Katagiri, Yuki Matsuzaka, Yohei Takeuchi, Miho Hatanaka, Takuro Kanekura, Sora Takeuchi, Takafumi Kadono, Yuya Fujita, Kiyoshi Migita, Takahiro Fujino, Takahiko Akagi, Tomoyuki Mukai, Tohru Nagano, Mitsuhiro Kawano, Hayato Kimura, Yukari Okubo, Akimichi Morita, Michihiro Hide, Takahiro Satoh, Akihiko Asahina, Nobuo Kanazawa, Kenji Kabashima
Summary: Schnitzler syndrome is a rare disorder characterized by chronic urticaria. A nationwide survey was conducted in Japan to determine the current status of the disease. Clinical information of 36 diagnosed cases was collected, providing insights into the clinical features and treatment of the syndrome. Continuous collection and long-term follow-up of clinical information are crucial for the appropriate management and further understanding of Schnitzler syndrome.
ALLERGOLOGY INTERNATIONAL
(2023)
Review
Immunology
Yuki Honda Keith, Gyohei Egawa, Tetsuya Honda, Kenji Kabashima
Summary: Mast cells (MCs) are immune cells that play important roles in maintaining homeostasis and inflammatory states in tissues. They are involved in the pathogenesis of type 2 skin inflammation in atopic dermatitis (AD) through both pro- and anti-inflammatory effects. The activation of skin MCs by environmental factors like Staphylococcus aureus can lead to AD inflammation, while MCs also contribute to pruritus through IgE-dependent and -independent degranulation. On the other hand, MCs can suppress type 2 skin inflammation by promoting Treg expansion and enhancing skin barrier function.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Immunology
Tetsuya Honda, Kenji Kabashima, Jun Kunisawa
Summary: Lipid mediators act through specific receptors on cell surfaces and play physiological roles locally at their production sites. Recent research has shed light on the roles of these mediators in various diseases, including inflammatory skin conditions. They not only signal themselves, but also enhance the signaling of other chemical mediators. Manipulating their signaling has the potential to be a novel therapeutic approach for human skin diseases.
IMMUNOLOGICAL REVIEWS
(2023)
Article
Dermatology
Kenji Kabashima, Takayo Matsumura, Yoshiteru Hayakawa, Makoto Kawashima
Summary: The background data from the Japanese phase 3 study showed that nemolizumab in combination with topical treatments can reduce pruritus in atopic dermatitis. This post-hoc analysis found that improvements in pruritus were associated with improvements in eczema symptoms, sleep quality, and daily life.
JOURNAL OF DERMATOLOGICAL TREATMENT
(2023)
Letter
Dermatology
Satoshi Nakamizo, Teruasa Murata, Yoshihiro Ishida, Satomi Aoki, Takashi Sasaki, Akiharu Kubo, Kenji Kabashima
JOURNAL OF DERMATOLOGY
(2023)
Article
Pathology
Masakazu Fujimoto, Toshiya Miyake, Yo Kaku, Masahiro Hirata, Kenji Kabashima, Hironori Haga
Summary: This study aimed to resolve the ongoing debate on whether lymphocytic thrombophilic arteritis (LTA) is a separate disease or a type of polyarteritis nodosa (PAN). Immunohistochemistry analysis of skin biopsies from five LTA patients revealed that the majority of infiltrating cells were myelomonocytic cells rather than lymphocytes. Based on these findings, PAN was considered a more appropriate diagnosis for the five cases. Further investigation is needed to determine if histiocytoid PAN with immature myeloid cells is the same disease as conventional PAN with mature neutrophils.
Letter
Dermatology
Yui Hirano-Lotman, Toshiaki Kogame, Yo Kaku, Takayoshi Komatsu-Fujii, Yuichiro Endo, Takashi Nomura, Kenji Kabashima
JOURNAL OF DERMATOLOGY
(2023)
Article
Dermatology
Hitomi Nakaizumi, Naotomo Kambe, Hiroyuki Irie, Yo Kaku, Masakazu Fujimoto, Hajime Yoshifuji, Yasuhiro Kazuma, Kazumoto Katagiri, Takuro Kanekura, Kenji Kabashima
Summary: Schnitzler syndrome (SchS) is a rare autoinflammatory disease characterized by bone pain, recurrent fever, and other symptoms. A key feature of the syndrome is the presence of a persistent urticarial-like rash. Histopathological data suggests that neutrophilic epitheliotropism, or the tendency of neutrophils to infiltrate and affect epithelial tissue, can be a useful marker for diagnosing SchS and differentiating it from conventional urticaria. Understanding this characteristic is crucial for early diagnosis and appropriate treatment.
JOURNAL OF DERMATOLOGY
(2023)
