Article
Biochemistry & Molecular Biology
Kenji Watanabe, Shuichi Shibuya, Yusuke Ozawa, Toshihiko Toda, Takahiko Shimizu
Summary: The study showed that p53 regulated ROS-mediated apoptotic cell death and tumorigenesis, but did not affect ROS-mediated tissue degeneration in SOD-deficient models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Alan H. Baik, Augustinus G. Haribowo, Xuewen Chen, Bruno B. Queliconi, Alec M. Barrios, Ankur Garg, Mazharul Maishan, Alexandre R. Campos, Michael A. Matthay, Isha H. Jain
Summary: Oxygen is toxic across all three domains of life, but the molecular mechanisms underlying this toxicity are largely unknown. In this study, we investigate the cellular pathways affected by excess oxygen and find that it destabilizes a specific subset of Fe-S cluster (ISC)-containing proteins, leading to impaired synthesis and repair functions. We show that electron transport chain (ETC) is the most vulnerable to damage, resulting in decreased mitochondrial oxygen consumption and further tissue damage. This research has important implications for hyperoxia-related pathologies.
Article
Cell Biology
Wei Wei, Nicholas M. Riley, Xuchao Lyu, Xiaotao Shen, Jing Guo, Steffen H. Raun, Meng Zhao, Maria Dolores Moya-Garzon, Himanish Basu, Alan Sheng-Hwa Tung, Veronica L. Li, Wentao Huang, Amanda L. Wiggenhorn, Katrin J. Svensson, Michael P. Snyder, Carolyn R. Bertozzi, Jonathan Z. Long
Summary: There is a need to identify blood-borne factors that mediate tissue crosstalk during physical activity. This study used a cell-type-specific proteomic approach to map exercise training-regulated secretomes in mice, identifying over 200 previously unreported cell-type-secreted protein pairs. The study also found exercise-induced secretion of proteoforms of intracellular carboxylesterases from the liver, which had anti-obesity, anti-diabetic, and exercise performance-enhancing activities.
Article
Immunology
Yuan-Yi Zhang, Bai-Sui Feng, Huanping Zhang, Gui Yang, Qiao-Ruo Jin, Xiang-Qian Luo, Na Ma, Qin-Miao Huang, Li-Teng Yang, Guo-Hao Zhang, Da-Bo Liu, Yong Yu, Zhi-Gang Liu, Peng-Yuan Zheng, Ping-Chang Yang
Summary: Exposure to specific antigens induces apoptosis in Tregs, which can be prevented by regulating superoxide dismutase or suppressing reactive oxygen species.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2021)
Article
Neurosciences
Ruth E. Fulton, Jennifer N. Pearson-Smith, Christopher Q. Huynh, Timothy Fabisiak, Li-Ping Liang, Stefanos Aivazidis, Brigit A. High, Georgia Buscaglia, Timothy Corrigan, Robert Valdez, Takahiko Shimizu, Manisha N. Patel
Summary: The study suggests that neuron-specific mitochondrial superoxide production is sufficient to drive complex and catastrophic epileptic phenotype, emphasizing the role of SOD2 in influencing astrocytic response in a cell-nonautonomous manner.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Cell Biology
Brian H. Kim, Maciej Jeziorek, Hur Dolunay Kanal, Viorica Raluca Contu, Radek Dobrowolski, Steven W. Levison
Summary: Inhibiting ALK5 can reduce cell death and improve neurological function, potentially through increasing levels of autophagy markers.
Article
Microbiology
Donghyeun Kim, Moonyong Song, Eunsoo Do, Yoojeong Choi, James W. Kronstad, Won Hee Jung
Summary: It was found that mutants of Cryptococcus neoformans lacking vacuolar functions exhibited increased vacuolar fragmentation, and high concentrations of metal ions, elevated temperature, and treatment with antifungal drugs also led to increased vacuolar fragmentation. These results indicate that fungal vacuoles fragment in response to oxidative stress.
Article
Medicine, Research & Experimental
Ken-Ichiro Tanaka, Mikako Shimoda, Maho Kubota, Ayaka Takafuji, Masahiro Kawahara, Tohru Mizushima
Summary: Lecithinized superoxide dismutase (PCSOD) effectively ameliorates renal ischemia/reperfusion (I/R) injury by inhibiting ROS production, reducing markers of renal damage and fibrosis, and preventing the transition from acute kidney injury (AKI) to chronic kidney disease (CKD). This suggests that PCSOD could be a promising therapeutic agent for I/R-induced renal injury.
Article
Chemistry, Multidisciplinary
Namrata Singh, Somanathapura K. NaveenKumar, Motika Geethika, Govindasamy Mugesh
Summary: The study demonstrates that CeVO4 nanozyme can substitute the function of SOD1 and SOD2 in neuronal cells even when the natural enzymes are down-regulated. The nanozyme prevents mitochondrial damage by regulating superoxide levels and restores physiological levels of anti-apoptotic proteins. Additionally, it effectively prevents mitochondrial depolarization and improves cellular ATP levels under oxidative stress.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Daiyong Chao, Qing Dong, Zhixuan Yu, Desheng Qi, Minghua Li, Lili Xu, Ling Liu, Youxing Fang, Shaojun Dong
Summary: This study presents an effective antioxidative system for diabetic chronic wound healing by integrating a metal organic framework (MOF) nanozyme with antioxidant enzyme-like activity with a hydrogel (Gel), which can modulate the oxidative stress microenvironment and transition from the inflammation phase to the proliferation phase, comparable to a widely used clinical drug.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Shuang Xu, Zibo Kang, Kan Li, Xueying Li, Yanhe Zhang, Xue-Jiao Gao
Summary: Selenium deficiency induces iron death through oxidative stress. The production of large amounts of ROS activates the TLR4/NF-κB signaling pathway, leading to apoptosis and necrosis of the gastric mucosa.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Medicine, Research & Experimental
Jianhua Wan, Jiale Wang, Larry E. Wagner, Oliver H. Wang, Fu Gui, Jiaxiang Chen, Xiaohui Zhu, Ashley N. Haddock, Brandy H. Edenfield, Brian Haight, Debabrata Mukhopadhyay, Ying Wang, David Yule, Yan Bi, Baoan Ji
Summary: The activation of M3R in mice can induce acute pancreatitis and lead to chronic pancreatitis through repeated episodes. Moreover, M3R antagonist can alleviate the severity of pancreatitis in mice.
Article
Neurosciences
Hirofumi Yamashita, Okiru Komine, Noriko Fujimori-Tonou, Koji Yamanaka
Summary: Non-cell autonomous mechanisms play a role in the pathogenesis of ALS, with glial cells being involved, but the specific role of glial cells has not been fully elucidated. Using microarrays, this study examined mRNA expression changes in the spinal cords of mutant SOD1 transgenic mice, revealing that the majority of differentially expressed genes were highly expressed in microglia and involved in immunological reactions. Additionally, disease-associated microglial genes were upregulated, while homeostatic microglial genes were not, and abnormal expression patterns were predicted in astrocytes. This analysis provides valuable insights into the molecular pathologies and cellular crosstalk in the CNS under physiological and pathological conditions in neurodegenerative diseases.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Article
Behavioral Sciences
Camila Rigobello, Rodrigo Moreno Klein, Juliana Diosti Debiasi, Luis Guilherme Ursini, Ana Paula Michelin, Andressa Keiko Matsumoto, Decio Sabbatini Barbosa, Estefania Gastaldello Moreira
Summary: The study found that gestational and lactational exposure to PAR may alter behavioral and brain oxidative stress parameters in rat offspring, with male offspring being more susceptible. The findings suggest that PAR use during pregnancy and lactation could be a potential risk factor for neurodevelopmental disorders.
BEHAVIOURAL BRAIN RESEARCH
(2021)
Article
Virology
Jacob I. Ayers, Guilian Xu, Qing Lu, Kristy Dillon, Susan Fromholt, David R. Borchelt
Summary: Mutations in SOD1 associated with ALS cause misfolding and aggregation. This study investigated the transmission of different isolates of G93A-SOD1 ALS conformers in transgenic mice. The findings support the notion that SOD1 acts as a prion-like host protein in ALS.
Article
Reproductive Biology
Yusuke Ozawa, Kenji Watanabe, Toshihiko Toda, Shuichi Shibuya, Nobuaki Okumura, Naoki Okamoto, Yorino Sato, Ikko Kawashima, Kazuhiro Kawamura, Takahiko Shimizu
BIOLOGY OF REPRODUCTION
(2019)
Article
Biochemistry & Molecular Biology
Naotaka Izuo, Kazuma Murakami, Yoshitaka Fujihara, Masahiro Maeda, Takashi Saito, Takaomi C. Saido, Kazuhiro Irie, Takahiko Shimizu
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2019)
Article
Dermatology
Shuichi Shibuya, Kenji Watanabe, Gaku Tsuji, Masamitsu Ichihashi, Takahiko Shimizu
EXPERIMENTAL DERMATOLOGY
(2019)
Article
Nutrition & Dietetics
Shuichi Shibuya, Toshihiko Toda, Yusuke Ozawa, Mario Jose Villegas Yata, Takahiko Shimizu
Article
Biochemistry & Molecular Biology
Toshiaki Teratani, Kengo Tomita, Sachiko Toma-Fukai, Yutaro Nakamura, Toshimasa Itoh, Hikaru Shimizu, Yasunaga Shiraishi, Nao Sugihara, Masaaki Higashiyama, Takahiko Shimizu, Ikuo Inoue, Yasuhiro Takenaka, Ryota Hokari, Takeshi Adachi, Toshiyuki Shimizu, Soichiro Miura, Takanori Kanai
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Article
Biochemistry & Molecular Biology
Sudha Sharma, Susmita Bhattarai, Hosne Ara, Grace Sun, Daret K. St Clair, Shenuarin Bhuiyan, Christopher Kevil, Megan N. Watts, Paari Dominic, Takahiko Shimizu, Kevin J. McCarthy, Hong Sun, Manikandan Panchatcharam, Sumitra Miriyala
Article
Physiology
Nobuhiro Watanabe, Yoshihiro Noda, Taeko Nemoto, Kaori Iimura, Takahiko Shimizu, Harumi Hotta
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Taichi Fukunaga, Shuuichi Mori, Takuya Omura, Yoshihiro Noda, Yasunori Fujita, Ikuroh Ohsawa, Kazuhiro Shigemoto
Summary: Mitochondrial dysfunction is considered a major cause of sarcopenia, and its effects differ based on fiber type. Oxidative stress is associated with age-related muscle fiber atrophy, while specific mitochondrial changes may correlate with muscle quality rather than muscle mass.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Kenji Watanabe, Shuichi Shibuya, Yusuke Ozawa, Toshihiko Toda, Takahiko Shimizu
Summary: The study showed that p53 regulated ROS-mediated apoptotic cell death and tumorigenesis, but did not affect ROS-mediated tissue degeneration in SOD-deficient models.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuichi Shibuya, Kenji Watanabe, Yusuke Ozawa, Takahiko Shimizu
Summary: While XOR-mediated O-2(center dot-) production contributes to aging-like pathologies in mice, its involvement in age-related tissue damage induced by SOD1 loss remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shuichi Shibuya, Kenji Watanabe, Daiki Sakuraba, Takuya Abe, Takahiko Shimizu
Summary: Musculoskeletal disease associated with aging can be treated by increasing muscle mass and consuming functional foods. In the muscle-Sod2(-/-) mouse model, functional compounds and antioxidants can enhance motor function.
Article
Cell Biology
Naotaka Izuo, Nobuhiro Watanabe, Yoshihiro Noda, Takashi Saito, Takaomi C. Saido, Koutaro Yokote, Harumi Hotta, Takahiko Shimizu
Summary: This study suggests that insulin resistance may contribute to the development of Alzheimer's disease by causing cognitive impairment and deregulation of blood flow. The findings provide insights into the mechanism linking type 2 diabetes and Alzheimer's disease.
Article
Geriatrics & Gerontology
Yoshitaka Kondo, Hitoshi Aoki, Masato Masuda, Hiroki Nishi, Yoshihiro Noda, Fumihiko Hakuno, Shin-Ichiro Takahashi, Takuya Chiba, Akihito Ishigami
Summary: Nutritional requirements vary with life stage and the appropriate ratio of nutrients, especially proteins, for maintaining metabolic health in old age was investigated in mice. The moderate protein intake percentages of 25% and 35% were found to be necessary for maintaining metabolic health in middle-aged mice, similar to young mice.
Article
Multidisciplinary Sciences
Tsuyoshi Hashizume, Bo-Kyung Son, Sakiko Taniguchi, Koichi Ito, Yoshihiro Noda, Tamao Endo, Michiko Nanao-Hamai, Sumito Ogawa, Masahiro Akishita
SCIENTIFIC REPORTS
(2019)