Article
Plant Sciences
Suchismita Roy, Manjari Mishra, Gundeep Kaur, Supreet Singh, Nishtha Rawat, Prabhjeet Singh, Sneh L. Singla-Pareek, Ashwani Pareek
Summary: OsCYP2-P is an active cyclophilin isolated from wild rice that can grow in coastal saline regions. It plays a role in stress tolerance by maintaining ion homeostasis and affects the signaling and transport of auxin in rice.
PHYSIOLOGIA PLANTARUM
(2022)
Article
Plant Sciences
Qian Bai, Xuexue Chen, Zhenzhen Zheng, Jinjing Feng, Yanjun Zhang, Yuanyue Shen, Yun Huang
Summary: VPT1 plays an important role in plant development and fruit ripening, and it may also regulate the transport of soluble sugar in fruits. The VvVPT1 protein isolated from grape berries is located in the tonoplast and contains SPX and MFS domains. The expression of VvVPT1 is significantly increased during fruit ripening and induced by sucrose. These findings suggest that VPT1 positively regulates fruit ripening and affects sugar content, fruit firmness, and ripening-related gene expression.
HORTICULTURE RESEARCH
(2023)
Article
Cell Biology
Linda van den Berg, Krista Kokki, Sylvia J. Wowro, Konstantin M. Petricek, Onur Deniz, Catrin A. Stegmann, Marius Robciuc, Mari Teesalu, Richard G. Melvin, Anni I. Nieminen, Michael Schupp, Ville Hietakangas
Summary: The Mondo/ChREBP-Mlx gene regulatory network is crucial for sugar metabolism and survival on a high-sugar diet in Drosophila larvae. The clockwork orange (CWO) gene is a mediator of the repressive response to sugar feeding and plays a conserved role in repressing ribosome biogenesis genes.
Article
Biochemistry & Molecular Biology
Xiaoyang Wang, Huicong Meng, Yuxi Tang, Yashi Zhang, Yunxia He, Jinggeng Zhou, Xiangzong Meng
Summary: Plants under pathogen attack produce high levels of ethylene to induce defense responses. The rate-limiting enzyme ACS is crucial for ethylene biosynthesis. In this study, it was found that ethylene biosynthesis induced by the fungal pathogen Botrytis cinerea in Arabidopsis is negatively regulated by the ethylene signaling pathway. The ethylene response factor ERF1A acts downstream of ethylene signaling to suppress the expression of ACS2 and ACS6. ERF1A also upregulates defensin genes and increases Arabidopsis resistance to B. cinerea. Furthermore, ERF1A is phosphorylated by MPK3/MPK6 to enhance its functions in suppressing ethylene biosynthesis and inducing defensin gene expression.
JOURNAL OF GENETICS AND GENOMICS
(2022)
Article
Multidisciplinary Sciences
S. Duboux, J. A. Muller, F. De Franceschi, A. Mercenier, M. Kleerebezem
Summary: Bifidobacteria, the early colonizers of the human gastrointestinal system, are crucial for gut development and disease prevention. This study developed a fluorescent protein-based reporter system to investigate carbohydrate metabolism in B. longum NCC 2705. The results revealed the induction of specific promoters by different carbohydrates and highlighted the complexity of carbohydrate consumption patterns in this strain.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Tsubasa Shoji, Kazuki Saito
Summary: This study provides insights into the molecular mechanism of SGA biosynthesis and its regulation in eggplant, offering a theoretical basis for further understanding of the anti-nutritional defense in eggplant plants.
Article
Plant Sciences
Azkia Khan, Jintao Cheng, Anastasia Kitashova, Lisa Fuertauer, Thomas Naegele, Cristiana Picco, Joachim Scholz-Starke, Isabel Keller, H. Ekkehard Neuhaus, Benjamin Pommerrenig
Summary: The ERDL4 protein in Arabidopsis is involved in fructose allocation and regulation of monosaccharide balance in the vacuolar membrane. Overexpression of ERDL4 increases total sugar levels by upregulating TST2 expression. ERDL4 activity is regulated by diurnal rhythm and cold acclimation and contributes to plant organ development and stress tolerance.
Article
Chemistry, Multidisciplinary
Karin Mannerstedt, Narendra Kumar Mishra, Ebbe Engholm, Morten Lundh, Charlotte S. Madsen, Philip J. Pedersen, Priska Le-Huu, Soren L. Pedersen, Nina Buch-Manson, Bjorn Borgstrom, Thomas Brimert, Lisbeth N. Fink, Keld Fosgerau, Niels Vrang, Knud J. Jensen
Summary: A glucose responsive insulin (GRI) has been developed using glucose cleavable linkers based on hydrazone and thiazolidine structures, demonstrating glucose responsiveness in vitro, particularly for thiazolidines. Clamp studies confirmed increased glucose infusion at hyperglycemic conditions for one GRI, indicating a true glucose response. The glucose responsive cleavable linker in these GRIs drives the release of active insulin from a circulating depot in response to changes in glucose levels.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Plant Sciences
Tengfei Liu, Md Abu Kawochar, Shahnewaz Begum, Enshuang Wang, Tingting Zhou, Shenglin Jing, Tiantian Liu, Liu Yu, Bihua Nie, Botao Song
Summary: Cold-induced sweetening is a severe issue in the potato processing industry. This study identifies StTST1 as the sugar transporter with the highest expression in potato tubers during postharvest cold storage. Knockdown of StTST1 reduces sugar accumulation and improves the frying quality of cold-stored tubers. Suppression of StTST1 promotes starch synthesis and inhibits starch degradation, possibly through the down-regulation of beta-amylase StBAM1. These findings provide a new strategy to improve the quality of cold-stored potatoes and reduce acrylamide content in potato chips.
HORTICULTURE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Ping Chang, Hsin-Yu Hsieh, Shih-Long Tu
Summary: In this study, the splicing factor RBP45d was identified in Arabidopsis, functioning with PRP39a in temperature-mediated flowering. RBP45d participates in initial pre-mRNA splicing and modulates temperature-sensitive alternative splicing of FLOWERING LOCUS M to control flowering time in Arabidopsis.
Article
Plant Sciences
Mostafa K. Sarmast, Hassan Salehi
Summary: The research demonstrates that silver nanoparticles positively regulate tobacco growth by curtailing ethylene production and inhibiting the ethylene signaling pathway. High concentrations of AgNPs can induce hypersensitivity in tobacco seedlings, while sub-lethal concentrations lead to increased leaf weight, plant height, and root length. The study suggests that AgNPs inhibit various aspects of the ethylene signaling pathway dose-dependently.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Plant Sciences
Tsubasa Shoji, Koki Moriyama, Nicolas Sierro, Sonia Ouadi, Nikolai Ivanov, Takashi Hashimoto, Kazuki Saito
Summary: In this study, the researchers investigated the regulation of nicotine biosynthesis in tobacco and identified several low-nicotine tobacco varieties lacking specific transcription factors. They characterized the newly identified alleles and examined their influence on alkaloid contents and gene expression related to nicotine biosynthesis. They also demonstrated the effect of a distal genomic region deletion on the expression of a key transcription factor and the alkaloid phenotype. These findings provide new insights into the regulation of nicotine biosynthesis and offer genetic resources for breeding low-nicotine tobacco.
Article
Plant Sciences
Hsin-Yu Chi, Shang-Ling Ou, Mao-Chang Wang, Chin-Ying Yang
Summary: The increased frequency of heavy rains has led to submergence stress in rice paddies, severely affecting rice production. This study assesses the physiological responses and regulatory mechanisms of Ethylene-Response AP2/ERF factors in different rice cultivars under submergence conditions with osmotic stress.
Article
Forestry
Yi Zhang, Lusheng Xin, Julien Pirrello, Yongjun Fang, Jianghua Yang, Jiyan Qi, Pascal Montoro, Chaorong Tang
Summary: The study identified five Ethylene Response Factor (ERF) genes in the cDNA library of Hevea latex, with HbERF-IXc4 showing the strongest transactivation capacity and being most frequently screened. These ERFs mainly localized in the nucleus and four of them exhibited apparent transactivation activity. This indicates the critical involvement of ERFs, especially HbERF-IXc4, in the activation of HbSUT3 expression in latex after Ethrel treatment and the stimulated latex yield.
Article
Engineering, Chemical
Zhe Shu, Hao-zheng Li, Yu Shi, Dan-ying Zuo, Zhuan Yi, Cong-jie Gao
Summary: Sugar dissolved in solution stabilizes proteins and decreases denaturation. In this study, a novel isoporous membrane with dual sugar and temperature responsiveness was prepared, which showed decreased protein denaturation and improved separation efficiency.
JOURNAL OF MEMBRANE SCIENCE
(2023)