Article
Entomology
Kakeru Yokoi, Takuya Tsubota, Akiya Jouraku, Hideki Sezutsu, Hidemasa Bono
Summary: In this study, reference transcriptome data for the silkworm was established and detailed examination of gene expression in silkworm tissues was performed. The results contribute to understanding silkworm biology and promoting industrial applications.
Article
Genetics & Heredity
Juan Zhu, Yan-Rong Chen, Tao Geng, Shun-Ming Tang, Qiao-ling Zhao, Xing-Jia Shen
Summary: The study demonstrated that the BmShadow gene plays a crucial role in 20E synthesis in Bombyx mori, with a specific 14-amino acids region being essential for its function. Rescue experiments on BmShadow(-/-) larvae showed a significant decrease in 20E concentration after gene knockout, which could be restored to normal levels by feeding 20E analogues.
Article
Biochemistry & Molecular Biology
Yan-Hua Chen, Tao Jiang, Ayinuer Yasen, Bing-Yan Fan, Juan Zhu, Mei-Xian Wang, Ping Qian, Xing-Jia Shen
Summary: This study found that N6-adenosine methylation (m(6)A) is involved in the diapause regulation of bivoltine silkworms by changing the expression levels of mevalonate kinase (MK). These findings provide a clearer understanding of the role of environmental signals in the diapause regulation of bivoltine silkworms.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Entomology
Kohei Kakino, Hiroaki Mon, Takeru Ebihara, Masato Hino, Akitsu Masuda, Jae Man Lee, Takahiro Kusakabe
Summary: This study characterizes the gene expression patterns and function in the testis of Bombyx mori, revealing peculiar features in spermatogenesis. The transcriptome analysis identifies widespread gene expression and testis-specific genes in B. mori, and compares it with Drosophila melanogaster for a better understanding. The study fills a knowledge gap and provides a basis for future research in spermatogenesis.
Article
Multidisciplinary Sciences
Linrong Wan, Wenfu Xiao, Ziyan Huang, Anlian Zhou, Yaming Jiang, Bangxing Zou, Binbin Liu, Cao Deng, Youhong Zhang
Summary: This article systematically identified and analyzed small open reading frames (smORFs) in the silkworm, finding 738 highly reliable smORFs and 34,401 possible smORFs. It also identified differentially expressed and tissue-specific-expressed smORFs, which may be closely related to tissue characteristics and functions.
Article
Chemistry, Multidisciplinary
Lin Ma, Vivian Andoh, Zhongyuan Shen, Haiyan Liu, Long Li, Keping Chen
Summary: This study aimed to explore the long-term effects of magnesium oxide nanoparticles (MgO NPs) on silkworms. The results showed that MgO NPs had no significant negative impact on silkworm growth or adult tissues, but altered the expression of genes in the silk gland.
Article
Environmental Sciences
Shoumin Sun, Qiqi Chen, Gang Chen, Zhenzhen Chen, Kaiyun Wang, Hongyan Wang
Summary: In this study, a risk assessment of the neonicotinoid insecticide nitenpyram was conducted in relation to its impact on silkworms. The study confirmed the presence of risks when nitenpyram is sprayed around mulberry fields. Additionally, differentially expressed miRNAs and related signaling pathways were identified, indicating the potential mechanism underlying nitenpyram's toxic effect on silkworms. Moreover, nitenpyram was found to induce oxidative damage and upregulate Imd, suggesting its potential impact on the growth and stress response of silkworms.
Article
Food Science & Technology
Maria Cermeno, Carmen Bascon, Miryam Amigo-Benavent, Manuel Felix, Richard J. FitzGerald
Summary: Silkworm pupae hydrolysates exhibited significant antioxidant activity, with Alcalase and Prolyve hydrolysates having the highest scavenging activities, and Flavourzyme and Brewers Clarex hydrolysates showing enhanced ferric reducing antioxidant power. Peptides SWFVTPF and NDVLFF demonstrated the highest antioxidant activity in HepG2 cells.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biochemistry & Molecular Biology
Zhi-hao Su, Jun-li Lv, Qi Ou, Zi-qin Zhao, Kai-yi Zheng, Xiao-ying Zhang, Wen-qing Lai, Xue-yang Wang, Ming-jie Deng, Mu-wang Li
Summary: The white epidermis of silkworms is caused by uric acid crystals accumulation. Abnormal metabolism of uric acid reduces its production, resulting in a transparent or translucent phenotype. This study examined the metabolite changes following Bombyx mori nucleopolyhedrovirus (BmNPV) infection in two strains of silkworms (p50 and op50) and found that the uric acid pathway plays a critical role in resistance. Feeding with inosine, a metabolite in this pathway, significantly enhanced larval resistance and modulated other metabolic pathways. The results provide insights into the resistance mechanism of silkworms to BmNPV and offer new strategies for pest control.
INSECT MOLECULAR BIOLOGY
(2023)
Article
Biochemical Research Methods
Feifei Zhu, Dandan Song, Han Chen, Qi Tang, Shuhao Huo, Xiaoyong Liu, Keping Chen
Summary: Lipids have been suggested to play a crucial role in virus entry and infection. A comprehensive lipidomic analysis using UPLC-MS was conducted on susceptible and resistant silkworm strains, revealing key lipids associated with virus infection and immune response. The study highlights the relationship between lipid biosynthesis, viral resistance, and immune response in the host.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Entomology
Kai Chen, Xu Yang, Dehong Yang, Yongping Huang
Summary: This study provides functional analysis of the Spindle-E (Spn-E) gene in Bombyx mori, revealing its vital roles in gonadal development and gametogenesis. Loss of BmSpn-E expression resulted in transposon derepression, reduced germ cell number, increased apoptosis, and impaired gonadal development at the early larval stage.
Article
Multidisciplinary Sciences
Ryoma Tsuchiya, Aino Kaneshima, Masakazu Kobayashi, Maki Yamazaki, Yoko Takasu, Hideki Sezutsu, Yoshiaki Tanaka, Akira Mizoguchi, Kunihiro Shiomi
Summary: Diapause is a major developmental switch in insects evolved as a survival strategy under unfavorable environmental conditions. In the silkworm Bombyx mori, diapause is transgenerationally induced as a maternal effect, with progeny diapause determined by the mother's embryonic environmental temperature. The molecular mechanisms of diapause induction involve a hierarchical pathway regulated by gamma-aminobutyric acid (GABA)ergic and corazonin signaling systems.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Weiwei Zhou, Jindie Hong, Jinying Han, Fuchuan Cai, Qi Tang, Qian Yu, Guohui Li, Shangshang Ma, Xiaoyong Liu, Shuhao Huo, Keping Chen, Feifei Zhu
Summary: In this study, the role of glycosaminoglycan modifications, including heparan sulfate (HS) and chondroitin sulfate (CS), in Bombyx mori nuclear polyhedrosis virus (BmNPV) infection was investigated. The results showed that HS and CS are important attachment factors for BmNPV and targeting them can effectively inhibit viral infection.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Xi Zheng, Fengdan Liu, Min Shi, Shuo Li, Xiaofan Xie, Guannan Li, Xiaoning Zhang, Yong Zhu
Summary: This study focused on the influence of dimethoate (DMT) on the reproduction of silkworms. Through digital gene expression (DGE) and RT-qPCR analysis, the researchers identified differential gene expression changes in eggs laid by silkworms under DMT exposure. The results revealed that DMT mainly affected stress response, metabolic processes, and signal transduction in silkworms.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Ivana Daubnerova, Ladislav Roller, Honoo Satake, Chen Zhang, Young-Joon Kim, Dusan Zitnan
Summary: Insect ecdysis triggering hormones act on specific neurons in the central nervous system to activate the ecdysis sequence, with different ETH receptor subtypes showing distinct spatial and temporal expression patterns in the CNS to control various stages of the ecdysis process.
SCIENTIFIC REPORTS
(2021)
