Article
Biotechnology & Applied Microbiology
Hai-Yan Zhou, Jian-Bao Zhou, Xiao-Nan Yi, Yan-Mei Wang, Ya-Ping Xue, De-Shui Chen, Xin-Ping Cheng, Mian Li, Hong-Yan Wang, Kai-Qian Chen, Zhi-Qiang Liu, Yu-Guo Zheng
Summary: The newly developed endoglucanase CoCel5A shows strong adaptability to temperature and pH, with optimal activity at 55-75 degrees Celsius and in citric acid buffer with pH 4.0-5.0. CoCel5A exhibits high affinity and catalytic efficiency towards carboxymethyl cellulose sodium salt and beta-d-glucan, suggesting its potential applications in biotechnological and environmental fields.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Energy & Fuels
Youssef Bacila Sade, Camila Silva Goncalves, Sandra Mara Naressi Scapin, Guilherme Luiz Pinheiro, Roberto Becht Flatschart, Wanderley de Souza, Norton Heise, Ednildo de Alcantara Machado
Summary: This study reports the identification and characterization of a novel endoglucanase AfEG66 from the snail Achatina fulica, which shows high activity and stability against polysaccharide substrates, contributing to the improvement of production efficiency of green fuels.
BIOENERGY RESEARCH
(2022)
Article
Materials Science, Paper & Wood
Martin Nagl, Oskar Haske-Cornelius, Lukas Skopek, Alessandro Pellis, Wolfgang Bauer, Gibson Stephen Nyanhongo, Georg Guebitz
Summary: Paper, with an annual production of over 400 million tons, is the main product of the largest industrially implemented biorefinery process. Enzymes have been used to refine pulp, significantly reducing energy consumption. Different commercial refining enzyme formulations showed varying activities on model substrates, indicating the importance of understanding individual enzyme mechanisms.
Article
Genetics & Heredity
Ke-Ke Xu, Qing-Ping Chen, Jia-Yin Guan, Zi-Yi Zhang, Kenneth B. Storey, Dan-Na Yu, Jia-Yong Zhang
Summary: Lonchodinae, a subfamily of the Phasmatidae family, is rich in insect species with over 330 species across 40 genera. The phylogenetic relationships within Lonchodinae have been debated, but the sequencing of Eurycantha calcarata mitochondrial genome suggests a sister relationship with Phylliinae.
MITOCHONDRIAL DNA PART B-RESOURCES
(2021)
Article
Biotechnology & Applied Microbiology
Lucas de Souza Falcao, Thais Santiago do Amaral, Guilherme Bittencourt Brasil, Patricia Melchionna Albuquerque
Summary: The study optimized the production of endoglucanase using cupuacu shells as substrate by A. brasiliensis, significantly increasing enzyme activity and enabling application in the hydrolysis of lignocellulosic biomass.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Plant Sciences
Kyoung Rok Geem, Younho Song, Inhwan Hwang, Hyeun-Jong Bae, Dong Wook Lee
Summary: The study established a plant-based platform for producing active GtCel12A enzyme, demonstrating high solubility and efficient endoglucanase activity of the fusion protein. The results indicate potential use of the enzyme for extracting monomeric sugars from lignocellulosic biomass for bioethanol production.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Francisca Contreras, Christina Nutschel, Laura Beust, Mehdi D. Davari, Holger Gohlke, Ulrich Schwaneberg
Summary: Cellulases are crucial enzymes in various industries, and improving their thermostability is a challenge. The study compares Constraint Network Analysis (CNA) and directed evolution in identifying beneficial positions for enhancing thermostability in enzyme engineering campaigns. CNA proves to be an effective method in reducing experimental burden and improving thermostability.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Ajmal Khan, Haji Khan, Muhammad Faheem, Amir Zeb, Malik Badshah, Young Ryun Chung
Summary: The study focused on a strain of Martelella endophytica, isolated from the roots of Rosa rugosa, and its cellulase Cel5A which exhibited high degrading activity towards cellulose substrates. It showed maximum activity at 50 degrees Celsius and pH 4.5, and also exhibited salt tolerance. The enzyme's homology model structure suggested a stable and compact protein with potential active sites for cellulose degradation.
MOLECULAR BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Parmeshwar Vitthal Gavande, Arun Goyal
Summary: PsGH5A is a potential efficient endoglucanase with high affinity and compactness, which could be useful for lignocellulosic biomass saccharification in the renewable energy sector. This study provides valuable insights for the development of renewable energy sources.
JOURNAL OF MOLECULAR MODELING
(2023)
Article
Biotechnology & Applied Microbiology
Xinxin Li, Xusheng Yan, Meiying Ye, Chuang Song, Jingsi Gao, Jia Zhu, Yanping Liu
Summary: In this study, NaOH-cellulase pretreatment was used to effectively improve the anaerobic digestion (AD) of corn straw. The optimal conditions for NaOH-cellulase pretreatment were found to be 6.0% NaOH concentration and 0.10 g.g(-1) dry matter cellulase. NaOH-cellulase pretreatment significantly increased methane production and straw degradation rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Sreeramulu Bhuvaragavan, Thenozhiyil Reshma, Karuppiah Hilda, Mani Meenakumari, Kannan Sruthi, Ramanathan Nivetha, Sundaram Janarthanan
Summary: Cellulolytic enzymes in the larval gut of the banana pseudostem weevil show potential as an optimal resource for bioenergy production, with high activity and thermo-tolerance.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Materials Science, Paper & Wood
Hulya Kudug Ceylan, Yakup Ulusu, Sema Bilgin, Isa Gokce
Summary: Endoglucanases, which randomly break down cellulose chains, have broad industrial applications. In this study, the bacterial endoglucanase gene yhfE was cloned and expressed in E. coli, with further analysis conducted using PCR, restriction enzyme analysis, and DNA sequencing. The production yield of yhfE-TolAIII was approximately 82 mg/L, and the recombinant yhfE was characterized by MALDI-TOF mass spectrometry and CD analysis.
CELLULOSE CHEMISTRY AND TECHNOLOGY
(2021)
Article
Agricultural Engineering
Yu Li, Jing Zhao, Zhenhua Zhang
Summary: Bioaugmentation has a promotive effect on microbial community and function in anaerobic digestion, enhancing hydrolysis and methanogenesis efficiency, enriching beneficial microorganisms, promoting cellulase activity, and accelerating methane production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Yingying Cai, Heng Li, Guangfei Qu, Junyan Li, Nanqi Ren, Hongmei Zou, Yinghui Hu, Jinhua Zeng
Summary: The synergistic effect of cellulase pretreatment and microvoltage has a significant impact on anaerobic fermentation by cow dung. It increases biogas yield and improves lignocellulose degradation, with electricity promoting microbial and enzymatic activity.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Martha Maria Arevalos-Sanchez, Adrian Omar Maynez-Perez, Felipe A. Rodriguez-Almeida, Jose Alfredo Martinez-Quintana, Fidel Alejandro Sanchez-Flores, Monserrath Felix-Portillo, America Chavez-Martinez, Myrna Elena Olvera-Garcia, Oscar Ruiz-Barrera, Agustin Corral-Luna
Summary: The study found that the recombinant enzymes egl-FZYE and cel-FZYE had optimal pH values of 6.0 and 6.5 at 39 degrees Celsius, with activities of 1051.65 and 607.80 U/mg, respectively. Both enzymes demonstrated the ability to degrade agricultural wastes, with an improved effect when used in combination, leading to a 60% increase in glucose release.
Article
Virology
Matan Shelomi, Wei Lin, Brian R. Johnson, Michael J. Furlong, Kayvan Etebari
Summary: The study found that the Vietnamese Walking Stick carried the deformed wing virus, possibly acquired through foodborne transmission.
Article
Entomology
Ching-Yu Shu, Hsueh-Lien Lai, Pei-Yin Lin, Kevin Chi-Chung Chou, Matan Shelomi
Summary: Smoke from burning plants is commonly used as a spatial repellent against insects and in religious rituals worldwide. Research shows that the chemical constituents of the smokes have repellent effects on mosquitoes, potentially reducing the impact of insect-vectored pathogens. Further investigation into isolating mosquito-repelling volatiles from incense without the smoke may provide a safer alternative.
PAN-PACIFIC ENTOMOLOGIST
(2021)
Correction
Multidisciplinary Sciences
Matan Shelomi, Etienne G. J. Danchin, David Heckel, Benjamin Wipfler, Sven Bradler, Xin Zhou, Yannick Pauchet
SCIENTIFIC REPORTS
(2021)
Letter
Computer Science, Information Systems
Matan Shelomi
Summary: This letter comments on a recent article by Laakso et al., which notes the disappearance of 176 open access journals from the Internet. The researchers suggest that these journals may have vanished because they were predatory, and were delisted in 2014.
JOURNAL OF THE ASSOCIATION FOR INFORMATION SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Matan Shelomi, Wen-Ming Chen, Hsin-Kuang Chen, Hsin-Ying Lee, Chiu-Chung Young, Shih-Yao Lin, Shwu-Jen Liaw
Summary: During an investigation of microbes associated with arthropods living in decaying coconut trees, a new species of Pseudomonas, Pseudomonas schmalbachii sp. nov., was identified from the digestive tract of the common Asian millipede. Genomic analysis showed that this isolate represents a distinct species from its closest relatives.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Review
Entomology
Matan Shelomi
Summary: This paper presents a scoping review of relevant case reports, research studies, and review articles on the use of vitamin B1 as an insect repellent. The findings suggest that vitamin B1 is not effective in repelling insects.
BULLETIN OF ENTOMOLOGICAL RESEARCH
(2022)
Article
Entomology
Ian M. Maher, Matan Shelomi
Summary: This study investigates whether insects follow Bergmann's Rule and examines the long-term changes in body size of a species of Scarabaeidae beetle in response to rising temperatures in Taiwan. The results show that the beetles' size has increased over time, with a correlation between size and rising yearly temperatures in Taipei populations.
ENVIRONMENTAL ENTOMOLOGY
(2022)
Editorial Material
Chemistry, Medicinal
Matan Shelomi
Summary: This letter comments on a recent article that claimed a complex hydrogel formulation of thiamine nanospheres is an effective topical insect repellent. However, the authors did not thoroughly review prior literature that found no evidence of repellency for thiamine. Additionally, the experiment described lacked negative controls, making the results inconclusive.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Entomology
Matan Shelomi
Summary: Cytochrome P450s are a group of detoxification enzymes found in all animals. This study examined the expression of different cytochrome P450s in the digestive tracts of six species of stick insects and their evolutionary patterns. The results showed a comparable or slightly limited complement of detoxification genes in stick insects, except for the presence of multiple enzymes per species in the subfamily CYP15A1. The functions of these expanded enzyme groups need further investigation.
Article
Microbiology
Matan Shelomi, Chiao-Jung Han, Wen-Ming Chen, Hsin-Kuang Chen, Shwu-Jen Liaw, Estelle Muehle, Dominique Clermont
Summary: Two new species of Chryseobacterium were identified through sequence analysis and whole-genome sequencing. The strains WLa1L2M3(T) and 09-1422(T) were found to have similarities but distinct differences from other Chryseobacterium species. This study provides evidence for the proposal of Chryseobacterium oryctis sp. nov. and Chryseobacterium kimseyorum sp. nov.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Anatomy & Morphology
Min-Rou Chiang, Matan Shelomi
Summary: Studying changes in the digestive tracts of beetles during pupation can provide new insights into insect metamorphosis, and the detailed observations made in this study can facilitate future research.
JOURNAL OF MORPHOLOGY
(2023)
Article
Ecology
Matan Shelomi, Shai Meiri
Summary: This review provides a practical guide for ecogeographic research, covering everything from hypothesis generation to result interpretation. By consolidating existing methodologies into a single document, it allows scientists at all levels and from any institution to carry out investigations on any biological rule, taxon, and location.
ECOLOGY AND EVOLUTION
(2023)
Article
Agriculture, Multidisciplinary
M. Shelomi
Summary: Interest in insects as food has increased globally over the last decade. Factors affecting willingness to eat insects in countries without traditions of edible insects have been studied predominantly in Europe, and with a predominant focus on consumer attributes. This study examined attitudes in Taiwan, using Diffusion of Innovations Theory as the framework, and found that food neophobia and levels of disgust or fear of insects had the strongest effects on respondent willingness and intent to eat insects.
JOURNAL OF INSECTS AS FOOD AND FEED
(2023)
Article
Entomology
Ching-Yu Shu, Matan Shelomi
Summary: Adding lures to mosquito ovitraps can enhance their efficiency, with fermented leaf infusions of Syzgium samarangense significantly increasing trap yield without negatively interacting with larvicides. This easily produced bait, made from readily available leaves in Taiwan, is a cost-effective solution for improving ovitrap performance.
JOURNAL OF MEDICAL ENTOMOLOGY
(2021)
Article
Entomology
Matan Shelomi, Chun-Ting Lin
Summary: In natural containers sampled from two locations in Taiwan, low mosquito and microbial diversity were observed, with variations over time. The study found that the presence of mosquitoes, including medically significant genera, did not seem to correlate with bacterial communities or plant species. Both mosquito and microbe communities in these containers vary too greatly with time and on a microgeographic scale to identify specific microbe-insect interactions using metabarcoding. Future research should focus on single-species experiments with cultured microbes, or long-term field studies covering all seasons.
INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE
(2021)
