Article
Anatomy & Morphology
Chan Wang, Bing Wang, Guirong Wang
Summary: This study investigated the sex pheromone recognition mechanism in the peripheral nervous system of Mythimna separata through electroantennogram recordings and single sensillum recording, revealing different types of trichoid sensilla with specific tuned neurons.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Multidisciplinary Sciences
Nicholas A. Steinmetz, Cagatay Aydin, Anna Lebedeva, Michael Okun, Marius Pachitariu, Marius Bauza, Maxime Beau, Jai Bhagat, Claudia Bohm, Martijn Broux, Susu Chen, Jennifer Colonell, Richard J. Gardner, Bill Karsh, Fabian Kloosterman, Dimitar Kostadinov, Carolina Mora-Lopez, John O'Callaghan, Junchol Park, Jan Putzeys, Britton Sauerbrei, Rik J. J. van Daal, Abraham Z. Vollan, Shiwei Wang, Marleen Welkenhuysen, Zhiwen Ye, Joshua T. Dudman, Barundeb Dutta, Adam W. Hantman, Kenneth D. Harris, Albert K. Lee, Edvard Moser, John O'Keefe, Alfonso Renart, Karel Svoboda, Michael Hausser, Sebastian Haesler, Matteo Carandini, Timothy D. Harris
Summary: The research introduces the Neuropixels 2.0 probe and newly designed analysis algorithms, enabling high-quality recordings for long time scales in small mammals. Automatic post hoc correction for brain movements allows recording from the same neurons for more than 2 months. These probes and algorithms enable stable recordings from thousands of sites during free behavior.
Article
Physiology
Twinkle Biswas, Jothi Kumar Yuvaraj, Bill S. Hansson, Christer Loefstedt, Olle Anderbrant, Martin N. Andersson
Summary: This study investigated how striped ambrosia beetles use pheromones, host, and non-host volatiles to locate suitable host trees. Thirteen different olfactory sensory neuron classes were identified, with one class being most responsive to pheromones. Additionally, the beetles showed specific responses to volatiles from their obligate fungal mutualist and non-host plant volatiles.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Agronomy
Hao Guo, Xin-Lin Gong, Guo-Cheng Li, Bao-Tong Mo, Nan-Ji Jiang, Ling-Qiao Huang, Chen-Zhu Wang
Summary: In this study, six PRs were identified through transcriptome sequencing in the fall armyworm, showing varying responses to major and minor sex pheromone components. It was confirmed that S. frugiperda uses both SfruOR56 and SfruOR62 to detect the minor sex pheromone component Z7-12:Ac. Additionally, four sensilla types responsible for detecting pheromone compounds were characterized using single sensillum recording.
PEST MANAGEMENT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Andres Hernandez-Clavijo, Cesar Adolfo Sanchez Trivino, Giorgia Guarneri, Chiara Ricci, Fabian A. Mantilla-Esparza, Kevin Y. Gonzalez-Velandia, Paolo Boscolo-Rizzo, Margherita Tofanelli, Pierluigi Bonini, Michele Dibattista, Giancarlo Tirelli, Anna Menini
Summary: The COVID-19 pandemic highlighted our limited understanding of human olfactory physiology. By using acute slices of human olfactory epithelium and whole-cell patch-clamp technique, this study characterized the electrical properties of olfactory sensory neurons and supporting cells. The study also demonstrated the intact transduction cascade in this preparation through responses to odorant mixtures. This research provides valuable insights into human olfactory physiology and lays the foundation for future studies.
Article
Biochemistry & Molecular Biology
Istvan Taisz, Erika Dona, Daniel Munch, Shanice N. Bailey, Billy J. Morris, Kimberly I. Meechan, Katie M. Stevens, Irene Varela-Martinez, Marina Gkantia, Philipp Schlegel, Carlos Ribeiro, Gregory S. X. E. Jefferis, Dana S. Galili
Summary: In Drosophila, a specific olfactory channel processes male pheromones, promoting female courtship and repelling males. The processing streams extract qualitative and positional information, with sensory neurons and projection neurons encoding concentration differences and angular positions of males. The third circuit layer consists of 47 cell types with diverse input-output connectivity, enabling behavioral responses in specific ethological contexts through multisensory integration.
Article
Agronomy
Chan Wang, Sai Zhang, Meng-Bo Guo, Xiao-Qing Hou, Bin Yang, Gui-Rong Wang
Summary: The sex pheromones of the fall armyworm, Spodoptera frugiperda, show differences in composition and proportions in different geographical populations, but always contain Z9-14:OAc as the major component. The study identified seven components from pheromone gland extracts of female S. frugiperda in the Yunnan population from China. Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc were identified as the male antennal active components, with a ternary combination of Z9-14:OAc, Z7-12:OAc, and Z11-16:OAc at a ratio of 88:1:11 showing the strongest attractiveness to males in wind tunnel and field tests.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hao Guo, Ling-Qiao Huang, Xin-Lin Gong, Chen-Zhu Wang
Summary: Researchers compared the function of pheromone receptors (PRs) in Helicoverpa armigera and H. assulta by expressing their PR repertoires in Drosophila T1 neurons. All PRs of the two species, except for OR11, have been deorphanized using a Drosophila expression system and a large panel of pheromone compounds. This study provides a valuable reference for understanding the peripheral coding of pheromones.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Rodi Topci, Mihai Alevra, Erik H. U. Rauf, Danielle de Jong-Bolm
Summary: This study investigated the activity of olfactory receptor neurons and their axon terminals in olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While individual olfactory receptor neurons show diversity in their responses to the same odor stimulus at the soma level, the presynaptic calcium activity in the glomerulus is homogeneous. It was also found that by applying trains of olfactory stimuli with varying amino acid concentrations, it is possible to induce spatio-temporal presynaptic response inhomogeneities and visually distinguish different axon subgroups within the glomerulus.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Cheng-Yu Li, Karli Lawrence, John Merlo-Coyne, Scott A. Juntti
Summary: Pheromones, such as PGF(2 alpha), are vital in reproductive processes in multiple species. The hormones are necessary for pheromone signaling, however, the specific signaling pathways can be different across species. Our study using Astatotilapia burtoni found that adult males strongly prefer odors of fertile females and the attractiveness is mediated through a yet-undiscovered pheromone derived from PGF(2 alpha) metabolism. Unlike zebrafish, A. burtoni males do not exhibit sensitivity to PGF(2 alpha), but prefer females injected with PGF(2 alpha), indicating a different signaling mechanism.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Hao Guo, Ping-Ping Guo, Ya-Lan Sun, Ling-Qiao Huang, Chen-Zhu Wang
Summary: The study shows that both HarmPBP1 and HarmPBP2 are involved in the detection of Z11-16:Ald in the male antennae of Helicoverpa armigera. HarmPBP1 and HarmPBP2 were found to be localized in cells surrounding the olfactory receptor neurons expressing HarmOR13. However, they have little effect on the selectivity and response kinetics of HarmOR13.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Neurosciences
Marie-Elisabeth Lessmann, Cagdas Guducu, Bergoi Ibarlucea, Thomas Hummel
Summary: This study aimed to investigate whether HLA-associated peptides can elicit specific neuronal responses in human olfaction. Through testing the discrimination ability of peptides in the olfactory epithelium receptors and analyzing electrophysiological signals from participants, it was found that within this experimental setup, HLA-associated peptides do not produce specific olfactory activation in humans.
Article
Agronomy
Qiang Wang, Peng Xu, Simon Sanchez, Phil Duran, Felipe Andreazza, Rufus Isaacs, Ke Dong
Summary: This study evaluated the repellency of nine plant essential oil components on Drosophila species, finding that most of them showed repellency in a dose-dependent manner. Repellency was related to activation of multiple odorant receptors, with some components involving receptor-independent mechanisms.
PEST MANAGEMENT SCIENCE
(2021)
Article
Neurosciences
Alina Vulpe, Karen Menuz
Summary: Insects perceive most odors through two large families of olfactory receptors, the Odorant Receptors (ORs) and Ionotropic Receptors (IRs). Different tuning receptors and their co-receptors are expressed in various sensilla, with some evidence suggesting possible functional overlap between different co-receptors. The high conservation and co-expression of these co-receptors in chemosensory neurons suggest a potential partnership in other insects as well.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Biology
Sho Kurihara, Masayoshi Tei, Junichi Hata, Eri Mori, Masato Fujioka, Yoshinori Matsuwaki, Nobuyoshi Otori, Hiromi Kojima, Hirotaka James Okano
Summary: Combined high-field MRI and DTI analyses allow visualization of olfactory sensory neurons and their projections, providing olfactory nerve maps in humans, marmosets, and mice. This technique has the potential to be used clinically for the diagnosis of olfactory dysfunction.
COMMUNICATIONS BIOLOGY
(2022)