Mating Competitiveness of Anopheles arabiensis Males as a Function of Transgenic State and Genetic Similarity to Females

We conducted mating competitions between wild-type and heterozygous transgenic Anopheles arabiensis males that were produced by repeated backcrosses of a transposable element expressing the beta 2-tubulin eGFP marker into two genetic backgrounds. These competed for genetically similar or dissimilar females in ratios of 1:1:3. We analyzed the effect of genetic similarity, transgenic state and stock on mating frequency. We observed no differences in the competitiveness of the wild and transgenic heterozygotes, no effect of genetic relatedness, nor a clear benefit of the out-crossing strategy to increase competitiveness. A decrease in the development rate of all phenotypic classes was observed among progeny of transgenic males but the rate of adult emergence of transgenic individuals was in most cases slightly faster than wild-type siblings.