Article
Chemistry, Multidisciplinary
Cecilia Mariel Gallego, Agostina Mazzeo, Paola Vargas, Sebastian Suarez, Juan Pellegrino, Fabio Doctorovich
Summary: HNO, a biologically relevant reactive nitrogen species, is highly reactive and impossible to store. Researchers have used donor compounds to study its chemistry, and attempted to stabilize it by coordination to metal centers. Various strategies for its detection have been proposed due to its high reactivity and short lifetime.
Review
Chemistry, Analytical
Sebastien Allard, Frants R. Lauritsen
Summary: This review summarizes the progress in the use of MIMS for DBP analysis in water since 2010. It highlights advancements in instrumentation, quantification, and new applications in swimming pools, water treatment, mechanistic studies, and kinetic modelling. The review discusses the use of high resolution mass spectrometry for distinguishing between isobaric isomers, analysis of hydrophilic and less volatile DBPs using condensed phase MIMS, and quantification based on univariate and multivariate mathematical algorithms. MIMS has been proven to be a valuable tool for implementing water treatment strategies focusing on DBP mitigation.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Thuan Thai, Fei Zhong, Lei Dang, Enoch Chan, Jacqueline Ku, Ernst Malle, Carolyn L. Geczy, John F. Keaney, Shane R. Thomas
Summary: The study demonstrates that transcytosed MPO in endothelial cells activates eNOS by promoting phospholipase C-dependent calcium signaling and altering eNOS phosphorylation status. This activation occurs despite reduced cellular uptake of the eNOS substrate L-arginine. These findings suggest a compensatory signaling response in endothelial cells to maintain eNOS activity and NO production in the presence of MPO-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Minmin Guo, Mingjian Zhang, Shunkai Gao, Lu Wang, Jichuan Zhang, Zejian Huang, Yiyang Dong
Summary: A portable pyrolysis-membrane inlet mass spectrometry (PY-MIMS) approach was developed to rapidly and accurately identify the rubber content in Eucommia ulmoides gum. The method involves pyrolyzing the gum into small molecules, which are then diffusively transported for quantitative analysis. The results showed a limit of detection of 1.36 μg/mg and a recovery rate ranging from 95.04% to 104.96%. Compared to pyrolysis-gas chromatography (PY-GC), the average relative error was 1.153%, and the detection time was reduced to less than 5 minutes, demonstrating the reliability, accuracy, and efficiency of the procedure. This method has the potential to be used for precise identification of rubber content in natural rubber-producing plants.
Article
Biochemistry & Molecular Biology
Sophia Awais, Nouman Farooq, Sharmeen Ata Muhammad, Hamed A. El-Serehy, Farrah Ishtiaq, Mehwish Afridi, Hina Ahsan, Amin Ullah, Tariq Nadeem, Kishwar Sultana
Summary: The aim of this study was to improve the solubility and stability of aripiprazole by forming binary and ternary inclusion complexes with HP beta CD and LA. The addition of HP beta CD and LA inclusion complexes enhanced the stability and dissolution rate of aripiprazole. The preparation method and drug-to-polymer and amino acid ratio were critical factors in achieving high solubility and stability.
Article
Biochemistry & Molecular Biology
Mingcai Liang, Zhengxuan Wang, Hui Li, Bingxiao Liu, Lin Yang
Summary: This study explored the relationship between the inhibition of HNE accumulation and the depression of inflammation by examining the effects of L-arginine intake in growing rats. The results showed that L-arginine reduced hepatic accumulation of HNE and suppressed inflammation in rats. This anti-inflammatory effect was attributed to the upregulation of IL-10 and the downregulation of pro-inflammatory cytokines, as well as the inhibition of NF-kappa B activation via the downregulation of PI3K/Akt.
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Benedetta Porro, Sonia Eligini, Edoardo Conte, Nicola Cosentino, Nicolo Capra, Viviana Cavalca, Cristina Banfi
Summary: Clinical data suggests that low levels of circulating l-homoarginine (HArg) are linked to cardiovascular disease and mortality. Researchers have developed a targeted metabolomic approach to quantify a wide range of amino acids involved in the Arg/nitric oxide pathway, revealing an impairment in the pathway and a potential sex-dependent regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Piotr Kaczka, Katarzyna Kubicka, Amit Batra, Marcin Maciejczyk, Edyta Kopera, Justyna Bira, Tomasz Zajac
Summary: This study aimed to investigate the effect of simultaneous supplementation of beta-hydroxy-beta-methylbutyrate and L-Arginine alpha-ketoglutarate on lower limb power and muscle damage in medium distance runners aged 15.3 years old. The results suggested that the supplement combination may help prevent a reduction in jump performance during training, but showed no influence on markers of muscle damage or mood.
Article
Agriculture, Multidisciplinary
Duan Yao-ke, Su Yen, Han Rong, Sun Hao, Gong Hai-jun
Summary: This study reports the functional characteristics of CsNIP2;2 in cucumber, which was found to be a tandem repeat of CsNIP2;1 with different subcellular localization and selectivity filter compared to previously characterized silicon influx transporters in other plants.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2022)
Article
Medicine, Research & Experimental
Mona Norouzi, Azadeh Nadjarzadeh, Majid Maleki, Sayyed Saeid Khayyatzadeh, Saeid Hosseini, Mehdi Yaseri, Hamed Fattahi
Summary: The present study demonstrated that perioperative supplementation with a combination of Gln, Arg, and HMB enhances recovery, reduces myocardial injury, and decreases the time of hospital and ICU stay in cardiac surgery patients.
Article
Biochemical Research Methods
Jens Dyckmans, Wolfram Eschenbach, Reinhard Langel, Lars Szwec, Reinhard Well
Summary: This study aimed to modify existing methods for precise determination of N-15 values in ammonium and nitrate at natural abundance levels. The developed method showed high precision in analyzing concentrations of ammonium or nitrate, with the ability to determine N-15 values with accuracy better than 0.9 mUr.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2021)
Article
Physics, Atomic, Molecular & Chemical
Camila A. Virgen, James D. Fox, Peter Santariello, Jaime L. Winfield, Kenneth C. Wright, Guido F. Verbeck
Summary: There is a growing demand for the development and improvement of technology used for the detection and identification of Chemical Warfare Agents. The current detection technology used by the U.S. government, while sensitive, is not considered rapid. The Membrane Inlet Mass Spectrometric (MIMS) technology on a portable system has shown success in rapid analysis and identification of CWA simulants, outperforming current FTIR and LCD systems in terms of speed.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Integrative & Complementary Medicine
Yang-Yang Wang, Ning Zhou, Yan-Po Si, Zhi-Yao Bai, Meng Li, Wei-Sheng Feng, Xiao-Ke Zheng
Summary: A metabolomics study using UPLC-Q-TOF/MS was conducted to investigate the mechanism of Corallodiscus flabellatus extract on Alzheimer's disease (AD). The results showed significant anti-AD effects and identified potential biomarkers associated with AD metabolism. Urinary metabolomics analysis helped dissect the underlying mechanism of the intervention.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2021)
Article
Physics, Atomic, Molecular & Chemical
Sean Sebastian Hughes, Marcus M. K. Hughes, Rasmus Voersaa Jonsbo, Carsten Uhd Nielsen, Frants Roager Lauritsen, Bala Krishna Prabhala
Summary: Beer is a complex mixture of over 7700 compounds, with around 800 being volatile. The use of MIMS and KNIME software for analyzing and characterizing local Danish beers has shown promise in distinguishing beers from different micro-breweries, laying the groundwork for future analysis of beer volatome using MIMS.
EUROPEAN JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Pediatrics
Dipti Baskar, Saraswati Nashi, Srijithesh P. Rajendran, Gautham Arunachal, Rita Christopher, Girish B. Kulkarni, Survarna Alladi
Summary: L-2 hydroxy glutaric aciduria (L-2HGA) is an autosomal recessive neurometabolic disorder characterized by clinical and radiological features for diagnosis, with genetic analysis needed for confirmation in patients with unexplained developmental delay and typical MRI features.
JOURNAL OF PEDIATRIC NEUROLOGY
(2022)
Article
Pharmacology & Pharmacy
Virag Bogdandi, Tomoaki Ida, Thomas R. Sutton, Christopher Bianco, Tamas Ditroi, Grielof Koster, Hillary A. Henthorn, Magda Minnion, John P. Toscano, Albert van der Vliet, Michael D. Pluth, Martin Feelisch, Jon M. Fukuto, Takaaki Akaike, Peter Nagy
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Article
Pharmacology & Pharmacy
Christopher L. Bianco, Takaaki Akaike, Tomoaki Ida, Peter Nagy, Virag Bogdandi, John P. Toscano, Yoshito Kumagai, Catherine F. Henderson, Robert N. Goddu, Joseph Lin, Jon M. Fukuto
BRITISH JOURNAL OF PHARMACOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Vinayak S. Khodade, John P. Toscano
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Physiology
Gizem Keceli, Ananya Majumdar, Chevon N. Thorpe, Seungho Jun, Carlo G. Tocchetti, Dong Lee, James E. Mahaney, Nazareno Paolocci, John P. Toscano
JOURNAL OF GENERAL PHYSIOLOGY
(2019)
Article
Chemistry, Multidisciplinary
Vinayak S. Khodade, Blaze M. Pharoah, Nazareno Paolocci, John P. Toscano
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2020)
Article
Chemistry, Organic
Jessica Zarenkiewicz, Vinayak S. Khodade, John P. Toscano
Summary: Nitroxyl (HNO) has been studied for its reactivity with hydrogen sulfide (H2S) and hydropersulfides (RSSH), revealing that the latter are more efficient traps for HNO and result in the production of various dialkylpolysulfides. The interaction of HNO with sulfur beyond thiols remains relatively unexplored, suggesting further research is needed.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Vinayak S. Khodade, Sahil C. Aggarwal, Alexander Eremiev, Eric Bao, Sarah Porche, John P. Toscano
Summary: Researchers have developed small-molecule donors to efficiently release Hydropersulfides (RSSH) and uncover their potential physiological functions. The complex relationship among RSSH, hydrogen sulfide (H2S), and higher order polysulfides in biological systems poses challenges in distinguishing their activities. Further investigation is needed to understand the biological responses and develop optimal RSSH donors for specific tissues/pathologies.
ANTIOXIDANTS & REDOX SIGNALING
(2022)
Review
Biochemistry & Molecular Biology
Jon M. Fukuto, Cristina Perez-Ternero, Jessica Zarenkiewicz, Joseph Lin, Adrian J. Hobbs, John P. Toscano
Summary: The mechanisms of formation and degradation of S-Nitrosothiol (RS-NO) are not well understood. Hydropersulfides (RSSH) have been found to react with RS-NO, leading to its degradation and release of NO, potentially playing a key role in regulating steady-state RS-NO levels and serving as a storage form of NO.
Article
Biochemistry & Molecular Biology
Blaze M. Pharoah, Vinayak S. Khodade, Alexander Eremiev, Eric Bao, Ting Liu, Brian O'Rourke, Nazareno Paolocci, John P. Toscano
Summary: Hydrogen sulfide (H2S) has protective effects in cardiovascular disease, but its mechanism of action is still unclear. Recent studies have found that H2S can modulate protein activity through redox-based post-translational modifications. Other reactive sulfur species also exhibit cardioprotective action. Exogenous hydropersulfide donation is a superior approach compared to other reactive sulfur species and mechanical post-conditioning in protecting cardiac function and limiting infarct size.
Article
Chemistry, Organic
Sahil C. Aggarwal, Vinayak S. Khodade, Sarah Porche, Blaze M. Pharoah, John P. Toscano
Summary: In this study, strategies to release hydropersulfides (RSSH) utilizing photoremovable protecting groups were reported. The insertion of a self-immolative linker between the protecting group and RSSH enabled efficient and quantitative release of RSSH. By applying this method, intracellular delivery of RSSH was successfully achieved in breast cancer cells.
JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zijun Wu, Vinayak S. Khodade, Jean-Philippe R. Chauvin, Deborah Rodriguez, John P. Toscano, Derek A. Pratt
Summary: Hydropersulfides (RSSH) play important roles in vivo as scavengers of oxidants and electrophiles. They are highly reactive towards phospholipid-derived peroxyl radicals and can effectively inhibit ferroptosis. The reactivity of RSSH can be improved by designing specific precursors. RSSH and their precursors have been shown to inhibit ferroptosis in cell culture.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Biochemistry & Molecular Biology
Blaze M. Pharoah, Chengximeng Zhang, Vinayak S. Khodade, Gizem Keceli, Christopher McGinity, Nazareno Paolocci, John P. Toscano
Summary: In this study, researchers found that hydropersulfides (RSSH) supplementation can effectively protect against doxorubicin-induced cardiotoxicity while enhancing its anticancer effects. RSSH activates Nrf2-dependent pathways and PGC-1 alpha, leading to improved antioxidant defenses and mitochondrial function, as well as inhibition of apoptosis. Additionally, RSSH can potentiate the anticancer effects of doxorubicin by inducing reductive stress.
Editorial Material
Biochemistry & Molecular Biology
Vinayak S. Khodade, John P. Toscano
Article
Chemistry, Multidisciplinary
Vinayak S. Khodade, Sahil C. Aggarwal, Blaze M. Pharoah, Nazareno Paolocci, John P. Toscano
Summary: The discovery of alkylsulfenyl thiocarbonates as a new class of RSSH precursors has significant implications in biological systems, with the ability to release RSSH under physiologically relevant conditions and protect cells against oxidative stress. The release kinetics of RSSH from these precursors can be tuned through electronic modification, demonstrating the potential for increasing intracellular RSSH levels.
Review
Biochemistry & Molecular Biology
Lucia Alvarez, Christopher L. Bianco, John P. Toscano, Joseph Lin, Takaaki Akaike, Jon M. Fukuto
ANTIOXIDANTS & REDOX SIGNALING
(2017)
Article
Biochemistry & Molecular Biology
Michael Ramek, Jasmina Sabolovic
Summary: This paper investigates the geometries, energy landscapes, and magnetic parameters of copper(II) amino acid compounds using density functional theory calculations. The results show that certain conformers with histaminate-like and glycine-like modes have low Gibbs free energies and high metal-binding affinities. The predicted conformations reproduce the experimental electron paramagnetic resonance parameters.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Wei Tian, Wen Zhong, Zengyan Yang, Ling Chen, Shijie Lin, Yanping Li, Yuxing Wang, Peilin Yang, Xing Long
Summary: A series of novel dibutyltin complexes based on salen-like ligands were synthesized and characterized. Complex S03 showed excellent in vitro anticancer activity and induced cancer cell death through multiple mechanisms. This study reveals new mechanisms of organotin complexes and provides new insights into the development of organotin metal complexes as anticancer drugs.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Natalie C. Korkola, Martin J. Stillman
Summary: Many proteins require a metal cofactor for their function, and these metals play a role in protein folding. The study found that the metallation rates of different metal binding pathways are influenced by pH, with the less cooperative pathway being more affected by low pH conditions. Specific mixtures of structures are formed in the initial steps of metallation, and unfolding the disordered apo-MT structure hinders the formation of these preliminary structures. The compact conformation of the native apo-MT allows for rapid formation of metal-thiolate structures with high affinity, providing protection from oxidation.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Boxuan Yang, Xitong Song, Binju Wang
Summary: The diiron active site plays a crucial role in catalytic transformations in both biological and chemical systems. Recent advancements in the field include the synthesis of biomimetic diiron catalysts inspired by the active structure of soluble methane monooxygenase (sMMO), which have been successfully applied to the dehydrogenation of indolines.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Christina N. Banti, Angeliki A. Piperoudi, Catherine P. Raptopoulou, Vassilis Psycharis, Constantinos M. Athanassopoulos, Sotiris K. Hadjikakou
Summary: Two copper(I) polymorphs with mitochondria-targeting properties were successfully prepared via metal ion conjugation. These compounds showed promising activities in DNA binding, enzyme inhibition, and cancer cell inhibition. The molecular mechanisms of action were investigated.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2024)