Article
Microbiology
Peng Lu, Kai Wu, Hongxi Zhou, Haibing Yu, Ju Yuan, Lang Dong, Qiao Liu, Xiaoyan Ding, Wei Lu, Haitao Yang, Limei Zhu, Leonardo Martinez
Summary: Recent global guidelines recommend Mycobacterium tuberculosis antigen-based skin tests, such as the ESAT6-CFP10 (EC) skin test, as alternatives to the tuberculin skin test (TST) and QuantiFERON-TB Gold In-Tube test (QFT). However, little is known about the diagnostic value of these tests among persons living with HIV (PLHIV). This study aimed to assess the diagnostic accuracy of the EC skin test among PLHIV in China and found that it performs well regardless of BCG vaccination or CD4 count.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Immunology
Yuan Yuan, Lu Xia, Qiaoyu Wu, Xuhui Liu, Shuihua Lu
Summary: This study evaluated the accuracy of the ESAT6-CFP10 skin test (ECST) in the differential diagnosis of active tuberculosis (ATB). The results showed that the ECST has low sensitivity and specificity, and moderate consistency with the interferon-gamma release assay (IGRA).
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medical Laboratory Technology
Moon Jung Kim, Eun-Hye Ha, Young-Kwan Lee, Sang Joon Park, Chang Hyeok An, So-Yeon Park, Soo-Kyung Kim
Summary: This study used the newly developed ROP-ESAT6-CFP10 antigen for tuberculosis (TB) detection and compared it with existing interferon-gamma release assays (IGRAs). The ELISpot using ROP-ESAT6-CFP10 showed good agreement with T-SPOT.TB and QuantiFERON-TB Gold. The use of ROP technology can potentially lower the manufacturing cost of TB-specific antigens for IGRAs.
ANNALS OF CLINICAL AND LABORATORY SCIENCE
(2022)
Article
Immunology
Miao Xu, Wei Lu, Tao Li, Jingxin Li, Weixin Du, Qi Wu, Qiao Liu, Baodong Yuan, Jinbiao Lu, Xiaoyan Ding, Feng Li, Min Liu, Baowen Chen, Jiang Pu, Rongping Zhang, Xiuhong Xi, Rongguang Zhou, Zaoxian Mei, Ronghui Du, Lifeng Tao, Leonardo Martinez, Shuihua Lu, Guozhi Wang, Fengcai Zhu
Summary: The EC skin test showed high specificity and sensitivity in diagnosing tuberculosis infection, comparable to the T-SPOT.TB test, and was not affected by BCG vaccination.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Umi Zulaikha Mohd Azmi, Nor Azah Yusof, Jaafar Abdullah, Faruq Mohammad, Shahrul Ainliah Alang Ahmad, Siti Suraiya, Nurul Hanun Ahmad Raston, Fatin Nabilah Mohd Faudzi, Sachin K. Khiste, Hamad A. Al-Lohedan
Summary: A portable electrochemical aptamer-antibody based sandwich biosensor was successfully developed for Mycobacterium tuberculosis detection in clinical sputum samples, showing high specificity and sensitivity with a detection range of 5 to 500 ng/mL and a detection limit of 1.5 ng/mL. The sensor demonstrated good correlation with the culture method in sputum samples with 100% specificity and sensitivity.
Article
Biochemistry & Molecular Biology
Xiaonan Guo, Weixin Du, Junli Li, Jiaxin Dong, Xiaobing Shen, Cheng Su, Aihua Zhao, Yongge Wu, Miao Xu
Summary: The new skin reagent EC has favorable sensitivity and specificity, which can effectively distinguish between BCG vaccination and TB infection. EC-induced erythema and PPD-induced induration have similar characteristics in terms of inflammatory response, lymphocytes, and acute-phase proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Infectious Diseases
Yang Yang, Zhixiong Fang, Wei Huang, Haiming Zhang, Si Luo, Sha Lin, Shaojie Li, Shuihua Lu
Summary: This study aimed to evaluate the safety of the ESAT6-CFP10 (EC) skin test compared to the tuberculin skin test (TST). The results showed that the EC group had fewer adverse reactions than the TST group, and most of the reactions were mild or moderate and self-limiting.
BMC INFECTIOUS DISEASES
(2022)
Article
Biology
Ludmilla Dela Coletta Troiano Araujo, Daniel Ernesto Rodriguez-Fernandez, Susan Grace Karp, Ellen de Souza Marquez, Aline Cristina dos Santos, Manuel Hospinal-Santiani, Raphael Aparecido Boschero, Eliezer Lucas Pires Ramos, Vanete Thomaz-Soccol
Summary: A study evaluated the diagnostic potential of two antigens specific for Mycobacterium tuberculosis, CFP10 and ESAT6 recombinant proteins, and developed stable formulations, showing satisfactory results for TB testing with high specificity and sensitivity. The proposed formulations containing the ESAT6 and CFP10 recombinant antigens are suitable for large-scale trials.
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Joseph A. Haegele, Ramakrishna Boyanapalli, Jaya Goyal
Summary: As oligonucleotides (ONs) and similar nucleic acid therapeutic modalities enter development pipelines, there is a constant need to develop bioanalytical methods to address their unique challenges. This report presents a toolbox of novel techniques that can be easily implemented for existing hybridization-ligation enzyme-linked immunosorbent assay formats, improving the sensitivity and specificity of these assays. These methods enhance the performance of bioanalytical assays, facilitating the research and development of innovative oligonucleotide therapeutics.
NUCLEIC ACID THERAPEUTICS
(2022)
Article
Biochemical Research Methods
Masako Osawa, Takurou Akiya, Funa Ogawa, Takao Suzuki, Masaki Yamagami, Tadashi Umemoto, Akira Ideno
Summary: This study evaluated a new approach using probe alteration-linked self-assembly reaction (PALSAR) technology as a signal amplifier for nonenzymatic hybridization assay, to quantify antisense oligonucleotides (ASOs). The results showed that PALSAR quantification achieved high sensitivity (1.5 to 6 pg/ml), stability (86.8-119.1% and 88.1-113.1% intra-/interday accuracies), precision (<= 17.2%), and exhibited less than 1% crossreactivity towards 3'n-1, a metabolite with a single base difference. This method provides a highly sensitive and specific approach for metabolite discrimination and ASO detection.
Article
Immunology
Jordan L. Mitchell, Chris Wilson, Janet E. Alexander, Shelley G. Rhodes, Danielle A. Gunn-Moore, Jayne C. Hope
Summary: In order to facilitate the diagnosis of feline tuberculosis (TB), an optimized enzyme-linked immunosorbent assay (ELISA) was developed and tested. The ELISA showed a sensitivity of 64.3% and specificity of 100% when used on cats with culture-confirmed TB. When tested on cats with strongly suspected mycobacteriosis, the sensitivity was 32.9%. These results suggest that antibody-based diagnostics can be a useful adjunctive test for cases of TB missed by the IGRA.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Atefeh Pourdadashi, Razieh Rezaei Adriani, Seyed Latif Mousavi Gargari
Summary: The research isolated single-stranded DNA aptamers with high affinity for different bacterial genera using the SELEX method. Aptamers C65 and C50 showed the highest affinity towards targets in 20 rounds of cell-SELEX. The selected aptamers successfully detected clinical bacterial strains from meningitis patients, demonstrating the potential for developing diagnostic tools.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Pharmacology & Pharmacy
J. Tan, Yan Yin, Guang Hua, Lili Wang
Summary: This study aims to observe and analyze the diagnostic value of T-cell enzyme linked immunospot assay for tuberculosis infection. The results show that this method has ideal sensitivity and specificity in the diagnosis of pulmonary tuberculosis and extrapulmonary tuberculosis.
INDIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Sahar Hussein Abdalla Hekal, Amany N. Dapgh, Mai Badr-Eldien Abd-Elhafeez, Hassan Mohamed Sobhy, Fatma Ahmed Khalifa
Summary: This study compared different methods for diagnosing bovine tuberculosis and found that the use of polypeptide antigen in ELISA provides better diagnostic efficacy than the traditional PPD-B antigen. The IFN-gamma assay was found to be more sensitive than both SICTT and ELISA. It is recommended to use the IFN-gamma assay in parallel with SICTT to detect more positive animals earlier.