Article
Multidisciplinary Sciences
Kohji Yamada, Saya Motohashi, Tsunekazu Oikawa, Naoko Tago, Rei Koizumi, Masaya Ono, Toshiaki Tachibana, Ayano Yoshida, Saishu Yoshida, Masayuki Shimoda, Masahiro Oka, Yoshihiro Yoneda, Kiyotsugu Yoshida
Summary: The study reveals the role of E-Syt1 in mediating cytosolic protein secretion at the ER-PM contact sites in liver cancer cells. SEC22B vesicles tethered to the PM via Q-SNAREs for their secretion, while PKC delta secretion and tumorigenicity were regulated by the interaction with E-Syt1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Xueyi Nie, Boli Ma, Xiaotan Yuan, Mengyuan Li, Yueyang Liu, Yuxin Hou, Yi Yang, Jinrui Xu, Yujiong Wang
Summary: This article aims to explore the regulation of the NLRP3 inflammasome and pyroptosis by endoplasmic reticulum stress (ERS) in THP-1 macrophages infected with Mycobacterium bovis Bacillus Calmette-Guérin (BCG). The results showed that BCG infection induced ERS, NLRP3 inflammasome activation and pyroptosis in THP-1 macrophages, which was inhibited by the ERS inhibitor TUDCA.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Irma Saulle, Ivana Marventano, Marina Saresella, Claudia Vanetti, Micaela Garziano, Claudio Fenizia, Daria Trabattoni, Mario Clerici, Mara Biasin
Summary: The study demonstrates that recombinant human ERAP2 treatment reduces HIV-1 replication in monocyte-derived macrophages, leading to increased expression of pro-inflammatory cytokines and activation of inflammasomes. Additionally, ERAPs treatment triggers antiviral mechanisms and enhances phagocytosis in macrophages, suggesting their potential use in novel preventive and therapeutic strategies against HIV-1 infection.
JOURNAL OF IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Cui Ma, Yuanyuan Zheng, Xiaowei Liu, Weiyuan Zhang
Summary: The study found a possible correlation between polymorphism at the 96121524 position in the ERAP-1 gene and susceptibility to preeclampsia in pregnant women of Han nationality in Beijing.
CLINICAL AND EXPERIMENTAL HYPERTENSION
(2021)
Article
Gastroenterology & Hepatology
Qin Yang, Xi Chen, Yanfang Zhang, Sha Hu, Fengjiao Hu, Yongping Huang, Tengfei Ma, Heng Hu, Han Tian, Song Tian, Yan-Xiao Ji, Zhi-Gang She, Peng Zhang, Xiao-Jing Zhang, Yufeng Hu, Hailong Yang, Yufeng Yuan, Hongliang Li
Summary: The study demonstrated that RNF5 inhibits the progression of NASH by targeting HRD1 in the ubiquitin-mediated proteasomal pathway, providing new insights into the pathogenesis of NASH and potential strategies for prevention and treatment.
Article
Medicine, Research & Experimental
Sarah M. Graff, Stephanie R. Johnson, Paul J. Leo, Prasanna K. Dadi, Matthew T. Dickerson, Arya Y. Nakhe, Aideen M. McInerney-Leo, Mhairi Marshall, Karolina E. Zaborska, Charles M. Schaub, Matthew A. Brown, David A. Jacobson, Emma L. Duncan
Summary: Maturity-onset diabetes of the young (MODY) is a heterogeneous group of monogenic disorders caused by impaired pancreatic beta cell function. A nonsynonymous coding variant in the KCNK16 gene has been linked to MODY, affecting potassium channel function and leading to reduced insulin secretion.
Article
Endocrinology & Metabolism
Karina Rodrigues-dos-Santos, Gitanjali Roy, Derk D. Binns, Magdalena G. Grzemska, Luiz F. Barella, Fiona Armoo, Melissa K. McCoy, Andy Huynh, Jonathan Z. Yang, Bruce A. Posner, Melanie H. Cobb, Michael A. Kalwat
Summary: The small molecule SW016789 has time-dependent effects on endoplasmic reticulum (ER) stress and function in pancreatic islet beta cells. Acute treatment enhances insulin secretion, while chronic exposure transiently induces ER stress and inhibits secretory function in a reversible manner. SW016789 acts upstream of voltage-dependent calcium channels and potentiates nutrient-induced calcium influx.
Article
Immunology
Peng Xiao, Ziwei Hu, Jiaheng Lang, Tianyuan Pan, Randall Tyler Mertens, Huilun Zhang, Ke Guo, Manlu Shen, Hongqiang Cheng, Xue Zhang, Qian Cao, Yuehai Ke
Summary: A study found that mannose supplementation can improve the development and recovery of colitis. Mannose prevents pathological endoplasmic reticulum stress induced by inflammatory cytokines secreted by macrophages in intestinal epithelial cells by normalizing protein N-glycosylation. In addition, mannose reduces inflammatory activation of colonic macrophages and directly suppresses TNF-alpha production in macrophages by reducing glyceraldehyde 3-phosphate levels. Dysregulated mannose metabolism was also found in the colonic mucosa of patients with inflammatory bowel disease. Activating PMM2 activity with epalrestat further enhances the therapeutic effect of mannose.
CELLULAR & MOLECULAR IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hitomi Nakao, Akira Seko, Yukishige Ito, Masafumi Sakono
Summary: This study found that the endoplasmic reticulum protein ERp29 may act as a bridge linking the lectin chaperones calnexin and calreticulin, forming complexes such as CNX-CRT. This not only facilitates the binding of CNX and CRT, but also reveals a new function of ERp29.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Yaxin Fu, Wenming Ji, Quan Liu, Lin Zhang, Caina Li, Yi Huan, Lei Lei, Xuefeng Gao, Leilei Chen, Cunyu Feng, Liran Lei, Jiayu Zhai, Pingping Li, Hui Cao, Shuainan Liu, Zhufang Shen
Summary: This study evaluated the effects and underlying mechanism of voglibose on glycemic control and intestinal inflammation in type 2 diabetic mice. The results showed that voglibose improved glucose metabolism by enhancing GLP-1 secretion and ameliorated intestinal inflammation by reducing pro-inflammatory macrophages and increasing tight junction proteins. These findings suggest that voglibose has potential anti-inflammatory effects on the intestine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Mary E. Law, Elham Yaaghubi, Amanda F. Ghilardi, Bradley J. Davis, Renan B. Ferreira, Jin Koh, Sixue Chen, Sadie F. DePeter, Christopher M. Schilson, Chi-Wu Chiang, Coy D. Heldermon, Peter Norgaard, Ronald K. Castellano, Brian K. Law
Summary: Breast cancer mortality remains high, and there is a need for safer and more effective treatments. Disulfide bond Disrupting Agents (DDAs) have been identified as a potential class of anticancer compounds that specifically target EGFR and HER2 overexpressing cancer cells. The study found that DDAs act by downregulating EGFR, HER2, and HER3 and activating Death Receptors 4 and 5 (DR4/5). Further analysis revealed that AGR2, PDIA1, and ERp44 are the target proteins of DDAs. DDAs disrupt the mixed disulfide bonds between PDIA1, ERp44, and their client proteins, and enhance basal DR5 oligomerization by targeting AGR2 and ERp44.
Article
Immunology
Qinglong Guo, Jing Bi, Honghai Wang, Xuelian Zhang
Summary: EsxA, secreted by the ESX-1 secretion system, is a major virulence factor of Mycobacterium tuberculosis. EspC, an ESX-1 substrate, triggers ER stress-mediated apoptosis in macrophages, leading to inflammation, Ca2+ release, and bacterial survival. Understanding the role of EspC in Mtb-host interactions sheds light on complex host-pathogen interactions and mechanisms underlying pathogenesis mediated by the ESX-1 system.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Pharmacology & Pharmacy
Hang Su, Yu Mei, Shuangxue Luo, Haixia Wu, Yan He, Yasunaga Shiraishi, Pingping Hu, Richard A. Cohen, Xiaoyong Tong
Summary: The substitution of SERCA2 C674 thiol accelerates the development of atherosclerosis by inducing ER stress and inflammation. This study highlights the importance of SERCA2 C674 redox state in the context of atherosclerosis and proposes a novel therapeutic strategy to combat atherosclerosis.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lihua Luo, Zhenyu Luo, Junlei Zhang, Xu Liu, Jiaxin Huang, Sijie Wang, Hang Yin, Xuemeng Guo, Yilong Hu, Yichao Lu, Xinyu Shan, Huihui Liu, Yingying Shi, Yongzhong Du, Fuchun Yang, Jian You
Summary: This passage introduces a treatment strategy for acute lung injury/acute respiratory distress syndrome (ALI/ARDS) and pulmonary fibrosis (PF). By designing a multistage targeted nanomicelle loaded with endoplasmic reticulum stress inhibitor and anti-inflammatory drug, the balance of macrophage polarization and restoration of endoplasmic reticulum homeostasis can be achieved, alleviating inflammation and ultimately resolving ALI and preventing PF.
Article
Immunology
Garyfallia Gouna, Christian Klose, Mar Bosch-Queralt, Lu Liu, Ozgun Gokce, Martina Schifferer, Ludovico Cantuti-Castelvetri, Mikael Simons
Summary: Microglia play a key role in promoting myelin repair after demyelinating injury, with cholesterol esterification being essential for myelin debris clearance and lipid droplet generation. TREM2 is crucial for adapting to excess cholesterol exposure and regulating lipid droplet formation, ultimately impacting the innate immune response and remyelination.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Yorinobu Yasuda, Takeaki Arakawa, Yumi Nawata, Sayaka Shimada, Shinya Oishi, Nobutaka Fujii, Shinichi Nishimura, Akira Hattori, Hideaki Kakeya
BIOORGANIC & MEDICINAL CHEMISTRY
(2015)
Article
Developmental Biology
Hisanori Matsumoto, Yukiyasu Sato, Akihito Horie, Koh Suginami, Hirohiko Tani, Akira Hattori, Yoshihiko Araki, Kyosuke Kagami, Ikuo Konishi, Hiroshi Fujiwara
Article
Biochemistry & Molecular Biology
Yoshikuni Goto, Yuko Ogawa, Hiroki Tsumoto, Yuri Miura, Takahiro J. Nakamura, Kenji Ogawa, Yoshihiro Akimoto, Hayato Kawakami, Tamao Endo, Ryohei Yanoshita, Masafumi Tsujimoto
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2018)
Article
Multidisciplinary Sciences
Tatsuomi Shiga, Takahiro J. Nakamura, Chiaki Komine, Yoshikuni Goto, Yasushi Mizoguchi, Midori Yoshida, Yasuhiko Kondo, Maiko Kawaguchi
Article
Biochemistry & Molecular Biology
Yoshikuni Goto, Takahiro J. Nakamura, Kenji Ogawa, Akira Hattori, Masafumi Tsujimoto
JOURNAL OF BIOCHEMISTRY
(2019)
Article
Chemistry, Organic
Aya Yoshimura, Shinichi Nishimura, Takehiro Suzuki, Akira Hattori, Naoshi Dohmae, Taira Kato, Hideaki Kakeya
Article
Oncology
Xuebing Li, Akira Hattori, Senye Takahashi, Yoko Goto, Hiroshi Harada, Hideaki Kakeya
Review
Biochemistry & Molecular Biology
Hiroshi Fujiwara, Masanori Ono, Yukiyasu Sato, Kazuhiko Imakawa, Takashi Iizuka, Kyosuke Kagami, Tomoko Fujiwara, Akihito Horie, Hirohiko Tani, Akira Hattori, Takiko Daikoku, Yoshihiko Araki
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Yoshikuni Goto, Takahiro J. Nakamura, Kenji Ogawa, Akira Hattori, Masafumi Tsujimoto
FRONTIERS IN MOLECULAR BIOSCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Takuro Matsuoka, Chihiro Motozono, Akira Hattori, Hideaki Kakeya, Sho Yamasaki, Shinya Oishi, Hiroaki Ohno, Shinsuke Inuki
Summary: The study showed that the stereocenters at the 2'- and 3'- OH groups in the ribityl tail are crucial for MAIT cell activation, while the 4'- OH group does not significantly affect the regulation of MAIT cell activity. Furthermore, it was found that 5-OP-RU forms a covalent bond to MR1 in cells within 1 hour.
Review
Biochemistry & Molecular Biology
Masafumi Tsujimoto, Kazuma Aoki, Yoshikuni Goto, Atsushi Ohnishi
Summary: This study explores the molecular diversity and functions of the oxytocinase subfamily, suggesting that their intracellular or extracellular location determines their important roles in maintaining homeostasis, including regulating blood pressure, maintaining pregnancy, retaining memory, and trimming antigenic peptides.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Yuko Ogawa, Yoshihiro Akimoto, Mamoru Ikemoto, Yoshikuni Goto, Anna Ishikawa, Sakura Ohta, Yumi Takase, Hayato Kawakami, Masafumi Tsujimoto, Ryohei Yanoshita
Summary: The study found that extracellular vesicles (EVs) remained stable under simulated gastric acid conditions but partially lost membrane integrity under simulated pancreatic and bile acid conditions. While some associated proteins degraded, the activity of DPP IV was retained. EVs may release their contents in the intestine, potentially modulating the homeostasis of signal transduction pathways in the gastrointestinal tract.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Review
Pharmacology & Pharmacy
Masafumi Tsujimoto, Kazuma Aoki, Atsushi Ohnishi, Yoshikuni Goto
BIOLOGICAL & PHARMACEUTICAL BULLETIN
(2020)
Meeting Abstract
Oncology
Xuebing Li, Akira Hattori, Senye Takahashi, Yoko Goto, Hiroshi Harada, Hideaki Kakeya
MOLECULAR CANCER THERAPEUTICS
(2019)
