Article
Biochemistry & Molecular Biology
Juan Fan, Jiawei Shi, Yong Zhang, Junwei Liu, Chenyi An, Huaying Zhu, Peng Wu, Wei Hu, Rui Qin, Danmei Yao, Xin Shou, Yibing Xu, Zhou Tong, Xue Wen, Jianpo Xu, Jin Zhang, Weijia Fang, Jizhong Lou, Weiwei Yin, Wei Chen
Summary: This study demonstrates that NKG2D has the ability to distinguish different ligands through mechanical force, with force strengthening the binding with MICA significantly.
Article
Chemistry, Multidisciplinary
Takuya Nakashima, Riku Tanibe, Hiroto Yoshida, Masahiro Ehara, Miwa Kuzuhara, Tsuyoshi Kawai
Summary: Pathway-dependent chirality control in silver nanoclusters is achieved by using enantiomeric ligand, leading to the formation of chiral silver nanoclusters with intrinsic chirality. Guest binding to the nanoclusters' shell can reverse the stability between right- and left-handed nanoclusters, resulting in unprecedented chirality inversion.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Immunology
Andrea Kosta, Abdelilah Mekhloufi, Lorenzo Lucantonio, Alessandra Zingoni, Alessandra Soriani, Marco Cippitelli, Angela Gismondi, Francesca Fazio, Maria Teresa Petrucci, Angela Santoni, Helena Stabile, Cinzia Fionda
Summary: This study evaluated the role of the GAS6/TAM signaling pathway in the regulation of NKG2D ligand expression and NK cell recognition of MM. The results showed that GAS6, MERTK, and AXL depletion led to downregulation of MICA and inhibition of NKG2D-mediated NK cell degranulation. GAS6 derived from bone marrow stromal cells also increased MICA expression in MM cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Yu-Fan Liu, Yun Chiang, Feng-Ming Hsu, Chiao-Ling Tsai, Jason Chia-Hsien Cheng
Summary: The combination therapy of radiotherapy and HDAC inhibition increases the expression of NKG2D ligands in HCC cells, enhancing their susceptibility to NK cell-mediated cytotoxicity. This suggests the potential use of combined RT/HDACi and NK cell-directed immunotherapy for HCC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Nanoscience & Nanotechnology
Ruyan Feng, Lijuan Zhu, Xingyu Heng, Gaojian Chen, Hong Chen
Summary: Research has shown that short-chain glycopolymers demonstrate better binding ability to immune-related proteins and enhanced promotion of dendritic cell maturation compared to long-chain glycopolymers. By combining experimental characterization and theoretical simulation, a deeper understanding of the interaction between glycopolymers with different chain lengths and immune-related proteins is achieved, providing a solid theoretical basis for the design of new glycopolymer-based immune drugs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Urology & Nephrology
Kristin Voeing, Ulf Michgehl, Nils David Mertens, Cara Picciotto, Mee-Ling Maywald, Jonas Goretzko, Sofie Waimann, Kevin Gilhaus, Manuel Rogg, Christoph Schell, Juergen Klingauf, Yaroslav Tsytsyura, Uwe Hansen, Veerle van Marck, Aimee L. Edinger, Beate Vollenbroeker, Ursula Rescher, Daniela Anne Braun, Britta George, Thomas Weide, Hermann Pavenstaedt
Summary: This study investigates the role of Rab7 protein in proteinuric glomerular diseases. Depletion of Rab7 leads to the accumulation of various vesicular structures and the development of renal diseases in mice, flies, and cell lines. These findings suggest that disruptions in endocytic and autophagic processes may play a crucial role in regulating podocyte health and disease.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Cell Biology
Michelle L. Swift, Kate Beishline, Samuel Flashner, Jane Azizkhan-Clifford
Summary: This passage discusses the role of Sp1 in the cell cycle and its regulation of DSB repair pathway choice, favoring NHEJ repair. Sp1 is shown to recruit the NHEJ repair factor 53BP1 in G1 phase, while being evicted through phosphorylation in S phase, inhibiting HR repair.
Article
Plant Sciences
Shirin Akhter, Karl Johan Westrin, Nathan Zivi, Veronika Nordal, Warren W. Kretzschmar, Nicolas Delhomme, Nathaniel R. Street, Ove Nilsson, Olof Emanuelsson, Jens F. Sundstrom
Summary: This study investigates reproductive phase change in the gymnosperm Picea abies using a cone-setting acrocona mutant, revealing the involvement of PaSPL1 gene and miRNAs in regulating the transition shoots.
Article
Nutrition & Dietetics
Meng-Jie Tsai, Chung-Hao Li, Hung-Tsung Wu, Hsin-Yu Kuo, Chung-Teng Wang, Hsiu-Ling Pai, Chih-Jen Chang, Horng-Yih Ou
Summary: Although sugar substitutes are recommended for weight and glycemic control, studies show that artificial sweeteners have adverse effects on glycemic homeostasis. This study found that sucralose administration increased insulin secretion and decreased plasma glucose levels in mice. However, long-term sucralose consumption augmented high-fat diet-induced insulin resistance and glucose intolerance, and this effect was mediated through the T1R3-ERK1/2 signaling pathway.
Article
Biochemistry & Molecular Biology
Hong-Xia Wang, Qian Zhang, Jiayu Zhang, Rong Luan, Zhanfeng Liang, Liang Tan, Yanan Xu, Peng Zhang, Lei Zheng, Yong Zhao, Yu-Rong Qiu
Summary: CD74 plays a crucial role in regulating the development of medullary thymic epithelial cells (mTECs) in the thymus, positively controlling mTEC cellularity and maturation partially through activation of the canonical NF-kappa B signaling pathway.
Article
Pharmacology & Pharmacy
Qingping Xu, Yuhong Cao, Xiaoyi Zhong, Xue Qin, Jingyun Feng, Huan Peng, Yongshao Su, Zhichao Ma, Sigui Zhou
Summary: Our study investigated the cardioprotective effects of riboflavin in heart failure by activating SCAD and the DJ-1-Keap1-Nrf2 signaling pathway. In vivo, riboflavin improved cardiac function, reduced myocardial fibrosis, and inhibited oxidative stress and cardiomyocyte apoptosis. In vitro, riboflavin decreased reactive oxygen species and inhibited cell apoptosis. These effects were mediated through the restoration of FAD levels, SCAD expression, activation of DJ-1, and inhibition of the Keap1-Nrf2/HO1 pathway.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Biology
Yuki Makino, Yuki Kamiyama, J. B. Brown, Toshiya Tanaka, Ryusuke Murakami, Yuki Teramoto, Takayuki Goto, Shusuke Akamatsu, Naoki Terada, Takahiro Inoue, Tatsuhiko Kodama, Osamu Ogawa, Takashi Kobayashi
Summary: The study reveals that OPRK1 plays an important role in the progression of castration resistance in prostate cancer. Loss of OPRK1 function can delay the acquisition of castration resistance and inhibit the growth of castration-resistant prostate cancer.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Barbara Bonomelli, Stefano Busti, Enzo Martegani, Sonia Colombo
Summary: Previously, we found that an abnormal accumulation of activated Ras in mitochondria is associated with increased apoptosis. In this article, we confirm this correlation by showing that the lack of trehalose-6P-synthase, which triggers apoptosis in Saccharomyces cerevisiae, leads to the localization of active Ras proteins in mitochondria. Furthermore, we demonstrate that active Ras2 proteins, accumulated in mitochondria in response to acetic acid, are likely involved in regulated cell death, while constitutively localized Ras1 proteins in mitochondria may play a role in promoting cell survival. Finally, we reveal that active mitochondrial Ras proteins promote apoptosis through the cAMP/PKA pathway, demonstrated by characterizing the gpa2 Delta and cyr1 Delta mutants.
Article
Multidisciplinary Sciences
Mineon Park, Yong Jin Cho, Bora Kim, Young Jong Ko, Yuria Jang, Yeon Hee Moon, Hoon Hyun, Wonbong Lim
Summary: The study suggests that RANKL promotes migration and invasion of PCa cells through EMT, and inhibiting RANKL signaling may help prevent the metastasis of prostate cancer.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Galina Yurevna Zheleznyakova, Eliane Piket, Maria Needhamsen, Michael Hagemann-Jensen, Diana Ekman, Yanan Han, Tojo James, Mohsen Khademi, Faiez Al Nimer, Patrick Scicluna, Jesse Huang, Ingrid Kockum, Omid R. Faridani, Tomas Olsson, Fredrik Piehl, Maja Jagodic
Summary: The study found widespread changes in miRNAs and other sncRNAs in patients with multiple sclerosis, especially more pronounced in CSF cells, showing a striking contrast with changes in peripheral blood cells. This suggests that sncRNA-mediated mechanisms play an important role in regulating the transcriptome of pathogenic cells, primarily in the CNS target organ.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biophysics
Iryna Antal, Martina Koneracka, Martina Kubovcikova, Vlasta Zavisova, Alena Jurikova, Iryna Khmara, Maria Omastova, Matej Micusik, Monika Barathova, Lenka Jelenska, Ivana Kajanova, Miriam Zatovicova, Silvia Pastorekova
Summary: Antibody-modified magnetic nanoparticles were successfully prepared and demonstrated the ability to target cancer cells specifically by conjugating specific monoclonal antibodies on their surface. The internalization of these nanoparticles into tumor cells expressing hypoxic marker was confirmed, showing their potential for targeted cancer therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Oncology
Miriam Zatovicova, Ivana Kajanova, Monika Barathova, Martina Takacova, Martina Labudova, Lucia Csaderova, Lenka Jelenska, Eliska Svastova, Silvia Pastorekova, Adrian L. Harris, Jaromir Pastorek
Summary: Hypoxia in the tumor microenvironment is a major factor in cancer progression, and low levels of oxygen may indicate treatment failure. Carbonic anhydrase IX (CA IX) is a precise target in hypoxic tumors, and its presence contributes to the failure of standard therapy. Humanized antibodies against CA IX show promise as suitable therapies for hypoxic tumors, with the ability to modulate the tumor microenvironment, induce an immune response, and block metastasis formation.
CANCER & METABOLISM
(2022)
Article
Biochemistry & Molecular Biology
Jonas Eriksson Strom, Simon Kebede Merid, Jamshid Pourazar, Anders Blomberg, Anne Lindberg, Mikael Ringh, Michael Hagemann-Jensen, Tomas J. Ekstrom, Annelie F. Behndig, Erik Melen
Summary: DNA methylation patterns in chronic obstructive pulmonary disease (COPD) were investigated using an epigenome-wide association study on bronchoalveolar lavage (BAL) cells. The study identified differential methylation sites associated with COPD, and functional analysis revealed biologically plausible pathways and gene relationships. Integration with mRNA data showed a strong functional readout for relevant genes. The study also found colocalization between differential methylation sites and COPD-associated SNPs, suggesting joint genetic and epigenetic pathways related to the disease.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Hematology
Gabi Overgaard Ovlisen, Peter Thygesen, Karin Nana Weldingh, Esther Bloem, Soren Skov, Kasper Almholt, Karin Maria Lovgren, Carsten Dan Ley, Thomas Lindebo Holm
Summary: This study aimed to investigate if the immunosuppressive drug CTLA4-Ig can induce tolerance in haemophilia A (HA) rats receiving recombinant human coagulation factor VIII (rhFVIII) treatment. The results showed that co-administration of CTLA4-Ig prevented the formation of ADAs and preserved the pharmacological efficacy of rhFVIII.
Article
Biotechnology & Applied Microbiology
Michael Hagemann-Jensen, Christoph Ziegenhain, Rickard Sandberg
Summary: Smart-seq3xpress is a simplified and miniaturized version of the Smart-seq3 protocol, which allows high-throughput single-cell RNA sequencing from full transcripts while reducing reagent usage. Analysis of peripheral blood mononuclear cells using Smart-seq3xpress revealed a comprehensive atlas of common and rare cell types. Compared with droplet-based methods, Smart-seq3xpress also provided additional coverage of cell-type-associated isoform variation.
NATURE BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Per Johnsson, Christoph Ziegenhain, Leonard Hartmanis, Gert-Jan Hendriks, Michael Hagemann-Jensen, Bjorn Reinius, Rickard Sandberg
Summary: This study utilized allele-sensitive single-cell RNA sequencing to investigate the transcriptional dynamics of long noncoding RNAs (lncRNAs). The results showed that lncRNAs have a longer duration between two transcriptional bursts compared to messenger RNAs. The study also observed increased cell-to-cell variability in lncRNA expression, which is attributed to lower burst frequencies producing larger quantities of RNA molecules. Furthermore, the study identified lncRNAs with cell state-specific functions involved in cell cycle progression and apoptosis and demonstrated their regulatory effects on nearby protein-coding genes' transcriptional bursting.
Article
Biochemical Research Methods
Christoph Ziegenhain, Gert-Jan Hendriks, Michael Hagemann-Jensen, Rickard Sandberg
Summary: This study introduces a strategy using molecular spikes with unique molecular identifiers (UMIs) to evaluate the accuracy of RNA counting in single-cell sequencing. The authors uncover and correct impaired counting methods, as well as improve estimates of cellular mRNA amounts. These molecular spikes and the accompanying software will benefit the validation of new methods and enable more accurate characterization of RNA counting in single-cell sequencing.
Article
Biochemistry & Molecular Biology
Cheng Zhao, Savana Biondic, Katherine Vandal, Asa K. Bjoerklund, Michael Hagemann-Jensen, Theresa Maria Sommer, Jesica Canizo, Stephen Clark, Pascal Raymond, Daniel R. R. Zenklusen, Nicolas Rivron, Wolf Reik, Sophie Petropoulos
Summary: The preconceptual, intrauterine, and early life environments can significantly impact the developmental trajectories and health outcomes of offspring. Assisted reproductive technologies using additives may alter blastocyst formation and affect the molecular profile and physiology of the embryo.
Article
Oncology
Miriam Zatovicova, Ivana Kajanova, Martina Takacova, Lenka Jelenska, Olga Sedlakova, Martina Labudova, Silvia Pastorekova
Summary: Carbonic anhydrase IX (CA IX) is an enzyme involved in tumor adaptation to low oxygen levels and acidic environments. This study shows for the first time that CA IX can be shed by ADAM10 in addition to ADAM17. ADAM10 acts non-redundantly to ADAM17 in cleaving the extracellular domain of CA IX.
Article
Biochemistry & Molecular Biology
Miriam Hanckova, Lucia Mihalikova, Silvia Pastorekova, Tatiana Betakova
Summary: Macrophages in infected tissue are involved in the immune response and can be influenced by hypoxia. In this study, it was found that hypoxia inhibits macrophage proliferation, downregulates the RIG-I-like receptor signalling pathway, and affects the expression of cytokines and pro-inflammatory factors.
Article
Multidisciplinary Sciences
Seong-Hwan Jun, Hosein Toosi, Jeff Mold, Camilla Engblom, Xinsong Chen, Ciara O'Flanagan, Michael Hagemann-Jensen, Rickard Sandberg, Samuel Aparicio, Johan Hartman, Andrew Roth, Jens Lagergren
Summary: Functional characterization of cancer clones is important for understanding cancer's evolutionary mechanisms. The authors introduce PhylEx, a method that combines bulk genomics and single-cell RNA sequencing data to reconstruct clonal trees and identify clones. PhylEx outperforms other methods and allows for accurate inference of clonal trees and analysis of cancer.
NATURE COMMUNICATIONS
(2023)
Article
Clinical Neurology
Dennis Klose, Maria Needhamsen, Mikael V. Ringh, Michael Hagemann-Jensen, Maja Jagodic, Lara Kular
Summary: Previous studies have shown accelerated epigenetic age in blood immune cells and glial cells of MS patients. This study found that lung immune cells also exhibit accelerated epigenetic aging, which is associated with smoking and occurs even in the absence of smoking in MS patients.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2023)
Meeting Abstract
Medicine, General & Internal
K. Babatunde, M. Ngara, M. Walch, B. Subramanian, M. Hagemann-Jensen, I. Ghiran, R. Sandberg, D. Irimia, P. Y. Mantel
SWISS MEDICAL WEEKLY
(2021)
Article
Virology
Ivana Kajanova, Lubomira Lukacikova, Lenka Jelenska, Katarina Grossmannova, Zofia Radikova, Miroslav Vlcek, Boris Klempa, Richard Kollar, Katarina Bodova, Juraj Kopacek, Silvia Pastorekova
Summary: A cross-sectional seroprevalence study conducted at the Slovak Academy of Sciences found that mRNA-based vaccines induce better humoral response, antibody levels reflect severity of COVID-19 symptoms, and post-COVID vaccination results in marked elevation of IgG levels. The study also revealed differences in seroprevalence among distinct research institutes, emphasizing the importance of time-dependent immunity waning and the spread of the more contagious Delta variant of SARS-CoV-2 in future pandemic management decisions.
