Article
Gastroenterology & Hepatology
Akane Hara, Tomohiro Watanabe, Kosuke Minaga, Tomoe Yoshikawa, Ken Kamata, Masatoshi Kudo
Summary: Research has found that interferon-alpha (IFN-alpha) and interleukin-33 (IL-33) serve as potential biomarkers for the diagnosis and monitoring of autoimmune pancreatitis (AIP) and immunoglobulin G4-related disease (IgG4-RD) activity after successful induction of remission. These biomarkers play a crucial role in identifying and treating these autoimmune disorders.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Immunology
Seung-Ji Kang, Ki-Jeong Park, Hye-Mi Jin, Young-Nan Cho, Tae Hoon Oh, Seong Eun Kim, Uh Jin Kim, Kyung-Hwa Park, Sook-In Jung, Tae-Ok Kim, Hyo Shin Kim, Young-Goun Jo, Jae Kyun Ju, Seung-Jung Kee, Yong-Wook Park
Summary: This study reveals that circulating pDCs and cDCs are numerically deficient and functionally impaired in scrub typhus patients. Changes in the expression levels of surface phenotypes of pDCs and cDCs may be influenced by pro-inflammatory cytokines.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tamara Gulic, Gordana Laskarin, Lana Glavan, Tanja Grubic Kezele, Herman Haller, Daniel Rukavina
Summary: The study investigated the binding of gp96 to Toll-like receptor (TLR) 4 and CD91 receptors on decidual CD1a(+) DCs in vitro, as well as the influence on the maturation status of CD1a(+) DCs. It was found that gp96 efficiently bound CD91 and TLR4 receptors, leading to the maturation of CD1a(+) DCs and significantly increasing the expression of INF-gamma and IL-15, which could potentially have detrimental effects on ongoing pregnancy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dermatology
J. Sulaimani, D. Cluxton, J. Clowry, A. Petrasca, O. E. Molloy, B. Moran, C. M. Sweeney, A. Malara, N. McNicholas, C. McGuigan, B. Kirby, J. M. Fletcher
Summary: DMF has been shown to increase the frequency of Treg cells and decrease Th17 cells in patients with psoriasis. It also inhibits T cell activation and proliferation in response to stimulation, while enhancing the resistance of Treg cells to oxidative stress induced by DMF.
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Annette Ives, Didier Le Roy, Charlotte Theroude, Juergen Bernhagen, Thierry Roger, Thierry Calandra
Summary: The cytokine MIF plays a chemokine-like role in promoting the migration of dendritic cells (DCs) by activating the Src/PI3K signaling pathway and myosin II complexes. MIF contributes to the recruitment of DCs to draining lymph nodes through autocrine and paracrine activities acting via CD74. These findings indicate that MIF is crucial for DC motility and trafficking.
Article
Oncology
Hang Yu, Ying Mei, Yang Dong, Chao Chen, Xianke Lin, Hailong Jin, Jiren Yu, Xiaosun Liu
Summary: The study aimed to investigate the role of CCR9-CCL25 interaction in the pDC-mediated immunosuppressive microenvironment of gastric cancer. The results showed that the expression of CCR9+CCL25 increased in gastric cancer tissue, and more CCR9+ pDCs were found in metastatic lymph nodes and carcinoma tissue, which were associated with advanced T staging and lymph node metastasis and conferred a poor prognosis. Therefore, CCR9-CCL25 interaction may play an important role in guiding pDCs to metastatic lymph nodes and carcinoma tissue, contributing to the formation of tumor immunosuppressive microenvironment and leading to a worse prognosis.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Immunology
Christina Johnson, Huy Quang Quach, Corinna Lau, Karin Ekholt, Terje Espevik, Trent M. Woodruff, Soren Erik Pischke, Tom Eirik Mollnes, Per H. Nilsson
Summary: Thrombin plays an important role in inflammation, but studying its effects on thromboinflammatory responses is difficult in common experimental models. In this study, the anticoagulant peptide GPRP was used to investigate the effects of thrombin on acute inflammation caused by Escherichia coli and Staphylococcus aureus. The results showed that thrombin could modulate bacterial-induced inflammation and had differential effects on cytokine production.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Geriatrics & Gerontology
Rosemary A. Hoffman, Sulan Huang, Geetha Chalasani, Abbe N. Vallejo
Summary: The study found that the absolute numbers of intraepithelial cells (iIEC) and plasmacytoid dendritic cells (pDC) are significantly lower in aged mice compared to young mice, despite an increase in the expression of C-C ligand 25 (CCL25). In both young and aged mice, the proportion and absolute number of intraepithelial pDC decreased during acute endotoxemia induced by lipopolysaccharide (LPS), potentially contributing to inadequate responses to gastrointestinal pathogens in aging.
Article
Biochemistry & Molecular Biology
Isabella Sanseverino, Arturo Ottavio Rinaldi, Cristina Purificato, Antonio Cortese, Enrico Millefiorini, Maria Cristina Gauzzi
Summary: Immune mechanisms play a crucial role in MS, and altered trafficking and activation of dendritic cells (DC) have been observed in MS patients. Interferon beta (IFN beta) and vitamin D modulate DC functions. The response to 1,25-dihydoxyvitamin D3 (1,25(OH)(2)D3) of DC obtained with IFN beta/GM-CSF (IFN-DC) and IL4-DC from different donor groups (MS patients and healthy donors) was studied. The differentiation protocol of DC affected the secretion of cytokines and chemokines involved in MS pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Soudeh F. Tehrani, Jean-Michel Rabanel, Samuel Legeay, Jerome Cayon, Jeremie Riou, Patrick Saulnier, Sylvie Marleau, V. Gaelle Roullin, Patrice Hildgen, Guillaume Bastiat
Summary: Polymer nanoparticles (NPs) have been extensively studied as drug delivery systems, but their effects on inflammatory processes are not well understood. This study investigated the impact of PEG chain length coating on inflammatory gene expression and ROS generation in vascular endothelial cells. The results showed that PEGylated NPs caused mild and transient activation of inflammatory genes and did not trigger ROS generation.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2022)
Article
Biochemistry & Molecular Biology
Sandra Gunne, Marie Schwerdtner, Marina Henke, Ann-Kathrin Schneider, Lucas Keutmann, Eva Boettcher-Friebertshaeuser, Susanne Schiffmann
Summary: The study found that TMPRSS2 has an impact on the activation of toll-like receptors in dendritic cells and cytokine release, but has minimal effect on chemokine receptors. TMPRSS2 seems to suppress cytokine release in cDCs, while possibly mediating cytokine release in pDCs, depending on the activated TLR.
Article
Multidisciplinary Sciences
Floriane Noel, Lucile Massenet-Regad, Irit Carmi-Levy, Antonio Cappuccio, Maximilien Grandclaudon, Coline Trichot, Yann Kieffer, Fatima Mechta-Grigoriou, Vassili Soumelis
Summary: ICELLNET is a transcriptomic-based framework that helps dissect cell communication by globally integrating cell-to-cell communication, quantifying communication scores, visualizing results, and connecting to reference human cell types, revealing the key role of cell communication.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Yange Wang, He Shi, Yulu Zhang, Qian Zeng, Tingmei Chen, Chengsen Chai
Summary: This study explored differentially expressed hub genes associated with tumor-infiltrating immune cells in a subtype of breast cancer called claudin-low breast cancer (CL-BCa), using a multi-approach bioinformatics analysis. The results showed an increase in tumor-infiltrating immune cells, especially CD8+ T cells and macrophages, in CL-BCa. Differentially expressed cytokines and claudins were found to be associated with overall survival. The study sheds light on the molecular features of CL-BCa in the immune microenvironment and contributes to the identification of prognosis biomarkers for CL-BCa patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Immunology
Biao Wang, Chuanmin Zhou, Qun Wu, Ping Lin, Qinqin Pu, Shugang Qin, Pan Gao, Zhihan Wang, Yingying Liu, Jacob Arel, Yanjiong Chen, Teng Chen, Min Wu
Summary: This study reveals the important role of cGAS in combating Pseudomonas aeruginosa infection. cGAS deficiency leads to increased bacterial burdens, severe lung injury, and excessive proinflammatory cytokine secretion. Lack of cGAS also results in the accumulation of mitochondrial DNA in the cytoplasm, which activates inflammasome and TLR9 signaling pathways, ultimately leading to excessive proinflammatory factor secretion.
Article
Immunology
Selma Tuzlak, Anne S. Dejean, Matteo Iannacone, Francisco J. Quintana, Ari Waisman, Florent Ginhoux, Thomas Korn, Burkhard Becher
Summary: Three decades ago, the description of helper T cell polarization led to a dramatic growth in the T cell universe, with new subsets emerging. Categorizing T helper cells based on the help they provide offers a better understanding of their complexity and diversity.
Article
Multidisciplinary Sciences
Yvonne M. Mueller, Thijs J. Schrama, Rik Ruijten, Marco W. J. Schreurs, Dwin G. B. Grashof, Harmen J. G. van de Werken, Giovanna Jona Lasinio, Daniel Alvarez-Sierra, Caoimhe H. Kiernan, Melisa D. Castro Eiro, Marjan van Meurs, Inge Brouwers-Haspels, Manzhi Zhao, Ling Li, Harm de Wit, Christos A. Ouzounis, Merel E. P. Wilmsen, Tessa M. Alofs, Danique A. Laport, Tamara van Wees, Geoffrey Kraker, Maria C. Jaimes, Sebastiaan Van Bockstael, Manuel Hernandez-Gonzalez, Casper Rokx, Bart J. A. Rijnders, Ricardo Pujol-Borrell, Peter D. Katsikis
Summary: This study demonstrates that by measuring anti-SARS-CoV-2 antibody and cytokine levels at the time of hospital admission and using unsupervised hierarchical clustering/machine learning, it is possible to predict unfavorable outcomes in COVID-19 patients.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, General & Internal
Emily Toscano-Guerra, Monica Martinez Gallo, Iria Arrese-Munoz, Anna Gine, Noelia Diaz-Troyano, Pablo Gabriel-Medina, Mar Riveiro-Barciela, Moises Labrador-Horrillo, Fernando Martinez-Valle, Adrian Sanchez Montalva, Manuel Hernandez-Gonzalez, Ricardo Pujol Borrell, Francisco Rodriguez-Frias, Roser Ferrer, Timothy M. Thomson, Rosanna Paciucci
Summary: This study analyzed the relationship between serum testosterone levels and immune cell skewing with disease severity in male COVID-19 patients. The study found quantitative differences in biochemical predictors of disease outcome between male and female patients. Longitudinal analyses identified serum testosterone trajectories as the strongest predictor of survival in male COVID-19 patients, and changes in serum luteinizing hormone (LH) and androstenedione levels were inconsistent with physiological feedback patterns.
Article
Immunology
Maria Belen Sanchez-Rodriguez, Erica Tellez, Mireia Casulleras, Francesc E. Borras, Vicente Arroyo, Joan Claria, Maria-Rosa Sarrias
Summary: This study analyzed the macrophage anti-inflammatory protein CD5L in the plasma extracellular vesicles (EVs) of patients with acute-on chronic liver failure (ACLF) and its relationship with lipid mediators. The circulating EV contents in ACLF were significantly altered, leading to a loss of anti-inflammatory and pro-resolving molecules involved in the control of acute inflammation.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Marta Clos-Sansalvador, Marta Monguio-Tortajada, Santiago Roura, Marcella Franquesa, Francesc E. Borras
Summary: Extracellular vesicles (EVs) have great potential in clinical application for disease research and biomarker development. However, standardized techniques for EV isolation and detailed reporting of methodology are crucial for ensuring reliable and comparable results.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Medicine, General & Internal
Monica Martinez-Gallo, Juliana Esperalba, Ricardo Pujol-Borrell, Victor Sanda, Iria Arrese-Munoz, Candela Fernandez-Naval, Andres Anton, Victoria Cardona, Moises Labrador-Horrillo, Tomas Pumarola, Manuel Hernandez-Gonzalez
Summary: This study aimed to assess the feasibility of a validated assay of T-cell responses as a complement to antibody tests for evaluating the levels of protection generated by natural infection or SARS-CoV-2 vaccines. The results showed that the IGRA test detected T-cell responses in naturally exposed and vaccinated individuals, and there was a high correlation with specific antibody levels. These findings suggest that the IGRA test could be a promising tool for measuring the state of the immune response to SARS-CoV-2.
Article
Immunology
Adrian Sanchez-Montalva, Daniel Alvarez-Sierra, Monica Martinez-Gallo, Janire Perurena-Prieto, Iria Arrese-Munoz, Juan Carlos Ruiz-Rodriguez, Juan Espinosa-Pereiro, Pau Bosch-Nicolau, Xavier Martinez-Gomez, Andres Anton, Ferran Martinez-Valle, Mar Riveiro-Barciela, Albert Blanco-Grau, Francisco Rodriguez-Frias, Pol Castellano-Escuder, Elisabet Poyatos-Canton, Jordi Bas-Minguet, Eva Martinez-Caceres, Alex Sanchez-Pla, Coral Zurera-Egea, Aina Teniente-Serra, Manuel Hernandez-Gonzalez, Ricardo Pujol-Borrell
Summary: Currently used laboratory tests for clinical management of COVID-19 patients have limited predictive power due to redundancy. This limitation could be overcome by adding immunological tests with independent predictive power.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Marta Clos-Sansalvador, Sergio G. Garcia, Miriam Moron-Font, Charles Williams, Niels-Christian Reichardt, Juan M. Falcon-Perez, Antoni Bayes-Genis, Santiago Roura, Marcella Franquesa, Marta Monguio-Tortajada, Francesc E. Borras
Summary: This study reveals the importance of N-glycosylation in MSC-EV for their interaction with endothelial cells and associated functions, such as cell uptake, migration, and angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Sergio G. Garcia, Noelia Sandoval-Hellin, Marta Clos-Sansalvador, Laura Carreras-Planella, Miriam Moron-Font, Dolores Guerrero, Francesc E. Borras, Marcella Franquesa
Summary: This study characterized B cell populations induced by MSC and identified specific biomarkers using RNAseq. The results showed that the induced B cell populations had immunomodulatory and extracellular matrix generation capabilities independent of IL-10.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Gara Arteaga-Henriquez, Jorge Lugo-Marin, Laura Gisbert, Imanol Setien-Ramos, Monica Martinez-Gallo, Ricardo Pujol-Borrell, Josep Antoni Ramos-Quiroga
Summary: Individuals with Autism spectrum disorder (ASD) exhibit differences in immune cell levels and inflammation markers compared to typically developing (TD) controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Mar Naranjo-Gomez, Mireia Pelegrin
Summary: This review summarizes recent studies reporting the induction of vaccinal effects by human immunodeficiency virus (HIV-1) antibody therapy. It also puts into perspective preclinical studies that have identified mechanisms involved in the immunomodulatory properties of antiviral antibodies. Finally, it discusses potential therapeutic interventions to enhance host adaptive immune responses in people living with HIV (PLWH) treated with broadly neutralizing antibodies (bNAbs).
CURRENT OPINION IN HIV AND AIDS
(2023)
Article
Biology
Marta Clos-Sansalvador, Marta Monguio-Tortajada, Ferran Grau-Leal, Vicenc Ruiz de Porras, Sergio G. Garcia, Marta Sanroque-Munoz, Miriam Font-Moron, Marcella Franquesa, Francesc E. Borras
Summary: This study evaluates the cell recruitment capacity using an agarose spot migration assay and finds different cell recruitment abilities of MSC-EVs and cancer EVs. The agarose spot migration assay provides a variety of measurements and migration settings, and has potential applications in the fields of regeneration and cancer metastasis.
Article
Biochemistry & Molecular Biology
Meritxell Llorens-Revull, Brenda Martinez-Gonzalez, Josep Quer, Juan Ignacio Esteban, Gonzalo Nunez-Moreno, Pablo Minguez, Idoia Burgui, Ricardo Ramos-Ruiz, Maria Eugenia Soria, Angie Rico, Mar Riveiro-Barciela, Silvia Sauleda, Maria Piron, Irene Corrales, Francesc E. Borras, Francisco Rodriguez-Frias, Ariadna Rando, Clara Ramirez-Serra, Silvia Camos, Esteban Domingo, Marta Bes, Celia Perales, Maria Isabel Costafreda
Summary: MicroRNAs (miRNAs) encapsulated in extracellular vesicles (EVs) have potential as diagnostic and prognostic biomarkers. This study aimed to find a reliable EV isolation method for miRNA sequencing, suitable for clinical applications. Results showed that SEC yielded the highest amount of EVs with good reproducibility, while GRAD and SEC+GRAD resulted in purer EV preparations. The NEBNext library exhibited the highest reproducibility in miRNA recovery and diversity. Therefore, combining GRAD EV isolation with NEBNext library preparation is suitable for miRNA detection using plasma samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Mireia Pelegrin, Soledad Marsile-Medun, Daouda Abba-Moussa, Manon Souchard, Mar Naranjo-Gomez
Summary: In addition to directly impacting viral propagation, antiviral mAbs can enhance the host's adaptive immune response and generate long-lasting protective immunity. These immunomodulatory effects rely on Fc-Fc gamma R interactions and can enhance humoral and cellular immune responses.
Meeting Abstract
Cell & Tissue Engineering
Marta Monguio-Tortajada, Cristina Prat-Vidal, Daina Martinez-Falguera, Micaela Munizaga-Larroude, Carolina Soler-Botija, Miriam Moron-Font, Adriana Cserkoova, Antoni Bayes-Genis, Francesc E. Borras, Santiago Roura, Carolina Galvez-Monton
TISSUE ENGINEERING PART A
(2022)
Article
Medicine, Research & Experimental
Marta Monguio-Tortajada, Cristina Prat-Vidal, Daina Martinez-Falguera, Albert Teis, Carolina Soler-Botija, Yvan Courageux, Micaela Munizaga-Larroude, Miriam Moron-Font, Antoni Bayes-Genis, Francesc E. Borras, Santiago Roura, Carolina Galvez-Monton
Summary: This study demonstrates the preclinical efficacy of locally delivering cATMSC-EV in an acute myocardial infarction (MI) pig model, showing improved cardiac function and reduced myocardial remodeling.
