Letter
Oncology
Elizabeth F. McInnes, David K. Meyerholz, Mark J. Arends
JOURNAL OF PATHOLOGY
(2023)
Article
Pediatrics
Sindhu Pandurangi, Seung Kim, Akihiro Asai, Alexander Bondoc, William Balistreri, Kathleen Campbell, Alexander Miethke, Anna Peters, Michael Rogers, Amy Taylor, Suzanna Labib Attia, Troy Gibbons, Bhargava Mullapudi, Rachel Sheridan, Greg Tiao, Jorge A. Bezerra
Summary: This study aimed to evaluate the efficacy of personalized use of antibiotics and corticosteroids in patients with biliary atresia after surgery. The results showed that the personalized medication group had a significantly higher success rate of bile drainage compared to the conventional treatment group, and also maintained more native livers at 2 years. Therefore, personalized medication may help improve postoperative bile drainage.
JOURNAL OF PEDIATRIC SURGERY
(2023)
Article
Anesthesiology
Joseph B. Lesnak, David S. Nakhla, Ashley N. Plumb, Alexandra McMillan, Sanjib Saha, Nikesh Gupta, Yan Xu, Pornpoj Phruttiwanichakun, Lynn Rasmussen, David K. Meyerholz, Aliasger K. Salem, Kathleen A. Sluka
Summary: Chronic pain is a major health problem that affects disability and quality of life. Current treatments for chronic pain are ineffective, so there is a need for new analgesics. In this study, the researchers tested the efficacy of selective androgen receptor modulators (SARMs) in a mouse model of muscle pain. They found that SARMs alleviated muscle hyperalgesia in both male and female mice, and this effect was mediated through androgen receptors. The SARM-loaded microparticles released the drug for a sustained period and did not produce toxic or rewarding effects. These findings suggest that SARM-loaded microparticles could be a potential therapy for chronic muscle pain.
Article
Pediatrics
Anna L. Peters, Michael Rogers, Gousia Begum, Qin Sun, Lin Fei, Daniel Leino, David Hildeman, Ervin Steve Woodle
Summary: This study retrospectively analyzed cases of late liver cellular rejection in children and found that higher levels of serum gamma-glutamyl transpeptidase, presence of autoantibodies, and increased CD8(+) T-cell infiltration are associated with delayed response to treatment for late cellular rejection.
PEDIATRIC TRANSPLANTATION
(2023)
Editorial Material
Surgery
Milad Rezvani, Kathleen M. Campbell, Carlos E. Prada, Anna L. Peters
Summary: Liver transplantation can transfer genetic defects in metabolic pathways, and this case highlights the occurrence of metabolic crisis and early allograft dysfunction due to an acquired argininosuccinate lyase deficiency. The recipient's genetic testing revealed a heterozygous mutation in the ASL gene, which encodes the urea cycle enzyme. Postoperative ischemia/reperfusion injury increased metabolic demand beyond the enzymatic capacity of the allograft. This case emphasizes the importance of considering occult metabolic variants in the allograft during early allograft dysfunction.
AMERICAN JOURNAL OF TRANSPLANTATION
(2023)
Article
Biochemistry & Molecular Biology
Athmane Teghanemt, Kara Misel-Wuchter, Jace Heath, Andrew Thurman, Priyanjali Pulipati, Garima Dixit, Ramasatya Geesala, David K. Meyerholz, Thorsten Maretzky, Alejandro Pezzulo, Priya D. Issuree
Summary: Regulatory T (T reg) cells are vital in maintaining tolerance and preventing fatal autoimmunity. This study reveals the importance of Tet enzymes and DNA demethylation in promoting Treg cell differentiation in the thymus. Tet3 is specifically involved in the development of CD4 single-positive thymocytes and plays a critical role in TCR-dependent IL-2 production, which is essential for chromatin remodeling and the upregulation of FoxP3.
Article
Pediatrics
Anna L. Peters, Seung Kim, Reena Mourya, Akihiro Asai, Amy Taylor, Michael Rogers, Kathleen Campbell, Lin Fei, Alexander Miethke, William F. Balistreri, Jorge A. Bezerra
Summary: This study evaluated the increase in the number of cases and the association with adenovirus in children with non-A-E severe acute hepatitis from late 2021 to early 2022. Despite a significant proportion of children with acute liver failure, the overall survival rate remains high.
JOURNAL OF PEDIATRICS
(2023)
Editorial Material
Pathology
David K. Meyerholz
VETERINARY PATHOLOGY
(2023)
Article
Biology
David K. Meyerholz, Mariah R. Leidinger, J. Adam Goeken, Thomas R. Businga, Sebastian Vizuett, Allison Akers, Idil Evans, Yan Zhang, John F. Engelhardt
Summary: Our objective was to qualify MUC5AC and MUC5B immunohistochemical techniques to provide a useful tool for studying mucin tissue in CF and other ferret models. Results showed that MUC5AC and MUC5B mucins were most commonly detected in large airways and least in small airways, consistent with goblet cell density in airway surface epithelia. We also found that stomach tissues were enriched in MUC5AC and gallbladder tissues were enriched in MUC5B, similar to human tissues. Qualified techniques for MUC5AC and MUC5B immunohistochemistry will be valuable for mucin tissue studies.
BMC RESEARCH NOTES
(2023)
Article
Chemistry, Multidisciplinary
Sudartip Areecheewakul, Andrea Adamcakova-Dodd, Zeb R. Zacharias, Xuefang Jing, David K. Meyerholz, Kevin L. Legge, Jon C. D. Houtman, Patrick T. O'Shaughnessy, Peter S. Thorne, Aliasger K. Salem
Summary: This study investigated the immunomodulatory effects of inhalation exposure to copper oxide nanoparticles (CuO NPs) in different immune conditions. The results showed that CuO NP exposure decreased T(H)1 and T(H)2 cells and increased T-bet(+) Treg cells in healthy mice. However, CuO NP inhalation exposure resulted in decreased T(H)2 responses and increased T-bet(+) Treg cells in mice with house dust mite (HDM) asthma, and increased T(H)2 cells in AIT-treated asthmatic mice.
Article
Oncology
Jordan L. Kohlmeyer, Joshua J. Lingo, Courtney A. Kaemmer, Amanda Scherer, Akshaya Warrier, Ellen Voigt, Juan A. Raygoza Garay, Gavin R. Mcgivney, Qierra R. Brockman, Amy Tang, Ana Calizo, Kai Pollard, Xiaochun Zhang, Angela C. Hirbe, Christine A. Pratilas, Mariah Leidinger, Patrick Breheny, Michael S. Chimenti, Jessica C. Sieren, Varun Monga, Munir R. Tanas, David K. Meyerholz, Benjamin W. Darbro, Rebecca D. Dodd, Dawn E. Quelle
Summary: Targeting CDK4/6 and MEK inhibitors in MPNSTs can synergistically activate tumor suppressor genes, induce cell death, and reduce tumor survival, while CDK4/6-MEK inhibition can enhance the efficacy of anti-PD-L1 therapy.
CLINICAL CANCER RESEARCH
(2023)
Article
Multidisciplinary Sciences
Feng Yuan, Grace N. Gasser, Evan Lemire, Daniel T. Montoro, Karthik Jagadeesh, Yan Zhang, Yifan Duan, Vitaly Ievlev, Kristen L. Wells, Pavana G. Rotti, Weam Shahin, Michael Winter, Bradley H. Rosen, Idil Evans, Qian Cai, Miao Yu, Susan A. Walsh, Michael R. Acevedo, Darpan N. Pandya, Vamsidhar Akurathi, David W. Dick, Thaddeus J. Wadas, Nam Soo Joo, Jeffrey J. Wine, Susan Birket, Courtney M. Fernandez, Hui Min Leung, Guillermo J. Tearney, Alan S. Verkman, Peter M. Haggie, Kathleen Scott, Douglas Bartels, David K. Meyerholz, Steven M. Rowe, Xiaoming Liu, Ziying Yan, Adam L. Haber, Xingshen Sun, John F. Engelhardt
Summary: This study demonstrates the crucial role of ionocytes in controlling airway surface liquid volume and mucociliary clearance through CFTR-dependent ion transport. The creation of conditional genetic ferret models enables the study of ionocyte biology and function, providing insights into human health and disease that have greater evolutionary conservation compared to traditional rodent models.
Article
Multidisciplinary Sciences
Pengfei Li, Biyun Xue, Nicholas J. Schnicker, Lok- Yin Roy Wong, David K. Meyerholz, Stanley Perlman
Summary: The unique region (SUD) in the nonstructural protein Nsp3 of SARS-CoV-2 plays an important role in enhancing viral translation. The study identified a attenuated virus with a mutation in the linker region of Nsp3 and a compensatory mutation in the nucleocapsid protein (N) that restored virulence, revealing the impact of Nsp3-N interactions on virus replication and pathogenesis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Lok-Yin Roy Wong, Abby Odle, Emma Luhmann, Douglas C. Wu, Yiquan Wang, Qi Wen Teo, Celeste Ptak, Alan Sariol, Shea Lowery, Matthias Mack, David K. Meyerholz, Nicholas C. Wu, Lilliana Radoshevich, Stanley Perlman
Summary: The internal proteins 8b and 9b of MERS-CoV and SARS-CoV-2 have different roles in mediating pathogenesis in infected animals. MERS-CoV lacking protein 8b expression is more virulent, while SARS-CoV-2 lacking protein 9b expression is attenuated. Additionally, mice infected with MERS-CoV lacking protein 8b expression showed increased levels of interferon and immune cell infiltration.
Article
Multidisciplinary Sciences
Abigail R. Gress, Christine E. Ronayne, Joshua M. Thiede, David K. Meyerholz, Samuel Okurut, Julia Stumpf, Tailor V. Mathes, Kenneth Ssebambulidde, David B. Meya, Fiona V. Cresswell, David R. Boulware, Tyler D. Bold
Summary: The OX40 protein is proposed as a marker for recently activated CD4 T cells at the infection site in tuberculosis, indicating its potential as a target for immunotherapy.
NATURE COMMUNICATIONS
(2023)