Article
Biology
Sabrina Adam, Julia Bracker, Viviane Klingel, Bernd Osteresch, Nicole E. Radde, Jens Brockmeyer, Pavel Bashtrykov, Albert Jeltsch
Summary: This study reveals that the catalytic activities of TET1 and TET2 are influenced by the flanking sequences of DNA substrates, and this influence is related to 5mC and 5hmC. Furthermore, enhanced flanking sequence preferences and profound effects on the specificity of TET2 are observed at non-CpG sites. The flanking sequence preferences of TET are reflected in genome-wide and local patterns of 5hmC and DNA demethylation.
COMMUNICATIONS BIOLOGY
(2022)
Review
Immunology
Scheherazade Sadegh-Nasseri
Summary: This article discusses the flexibility of MHC II molecules as a key factor in antigen selection and interactions with accessory molecules like HLA-DM and HLA-DO in antigen processing.
CURRENT OPINION IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guglielmo Vesco, Marco Lamperti, Domenico Salerno, Claudia Adriana Marrano, Valeria Cassina, Riccardo Rigo, Enrico Buglione, Maria Bondani, Giulia Nicoletto, Francesco Mantegazza, Claudia Sissi, Luca Nardo
Summary: Utilizing fluorescence resonance energy transfer, the folding of expressly designed constructs with G-quadruplexes flanked by double stranded DNA segments was investigated both in ensemble and at the single molecule level. The presence of flanking ends was found to bias both the final equilibrium state and the folding kinetics of the G-quadruplexes. This previously unconsidered aspect sheds light on the complex relationships involved in fine tuning the gene-regulatory properties of DNA structures.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Guglielmo Vesco, Marco Lamperti, Domenico Salerno, Claudia Adriana Marrano, Valeria Cassina, Riccardo Rigo, Enrico Buglione, Maria Bondani, Giulia Nicoletto, Francesco Mantegazza, Claudia Sissi, Luca Nardo
Summary: G-quadruplexes within gene promoters, particularly in the oncogene KIT, have been studied for their role in regulating gene expression. Research shows that the folding of these G-quadruplexes can be influenced by the presence of flanking ends. This previously overlooked aspect should be taken into consideration for a better understanding of the gene-regulatory properties of these DNA structures.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Engineering, Biomedical
Lu Shi, Jingxing Yang, Ying Nie, Yizhou Huang, Hongchen Gu
Summary: This study presents a hybrid nano vaccine composed of hyper branched poly(beta-amino ester), modified iron oxide nano adjuvant, and messenger RNA encoded with a model antigen. The nano vaccine outperforms other commercialized reagents in promoting dendritic cells' transfection, maturation, and peptide presentation. In combination with immune checkpoint blockades, the nano vaccine can induce robust tumor suppression.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Virology
Pedro Alves-Peixoto, Maria Ferez, Cory J. Knudson, Colby Stotesbury, Carolina R. Melo-Silva, Eric B. Wong, Margarida Correia-Neves, Luis J. Sigal
JOURNAL OF VIROLOGY
(2020)
Article
Immunology
Colby Stotesbury, Pedro Alves-Peixoto, Brian Montoya, Maria Ferez, Savita Nair, Christopher M. Snyder, Shunchuan Zhang, Cory J. Knudson, Luis J. Sigal
JOURNAL OF IMMUNOLOGY
(2020)
Article
Virology
Pedro Alves-Peixoto, Maria Ferez, Cory J. Knudson, Carolina R. Melo-Silva, Colby Stotesbury, Eric B. Wong, Margarida Correia-Neves, Luis J. Sigal
JOURNAL OF VIROLOGY
(2020)
Article
Cell Biology
Eric Wong, Brian Montoya, Colby Stotesbury, Maria Ferez, Ren-Huan Xu, Luis J. Sigal
Article
Microbiology
Eric B. Wong, Brian Montoya, Maria Ferez, Colby Stotesbury, Luis J. Sigal
Article
Immunology
Nicole A. Wilski, Colby Stotesbury, Christina Del Casale, Brian Montoya, Eric Wong, Luis J. Sigal, Christopher M. Snyder
JOURNAL OF IMMUNOLOGY
(2020)
Article
Cell Biology
Colby Stotesbury, Eric B. Wong, Lingjuan Tang, Brian Montoya, Cory J. Knudson, Carolina R. Melo-Silva, Luis J. Sigal
Editorial Material
Cell Biology
Colby Stotesbury, Luis J. Sigal
Article
Cell Biology
Jiao Lu, Shan Li, Xiaopeng Li, Wenming Zhao, Xuefeng Duan, Xiuling Gu, Jianqiao Xu, Bolan Yu, Luis J. Sigal, Zhongjun Dong, Lixin Xie, Min Fang
Summary: The study revealed the significant regulatory role of miR-181a-5p in young and aged NK cells, showing a change in miRNAs profile in NK cells with aging and contributing to impaired NK cell function.
Article
Immunology
Maria Ferez, Cory J. Knudson, Avital Lev, Eric B. Wong, Pedro Alves-Peixoto, Lingjuan Tang, Colby Stotesbury, Luis J. Sigal
Summary: During viral infection, recognition of Qa-1(b) by activating CD94/NKG2 receptors is not critical. Instead, the levels of Qa-1(b) expression are down-regulated in infected cells but increased in some bystander immune cells to respectively promote or inhibit their killing by activated NK cells.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
Article
Biotechnology & Applied Microbiology
Cory J. Knudson, Pedro Alves-Peixoto, Hiromi Muramatsu, Colby Stotesbury, Lingjuan Tang, Paulo J. C. Lin, Ying K. Tam, Drew Weissman, Norbert Pardi, Luis J. Sigal
Summary: Different doses of modified mRNA vaccines can induce strong CD8 T cell responses, providing protective immunity and increasing the memory CD8 T cell pool, which can be used to create CD8 T cell-based vaccines against viral infections.
Article
Virology
Cory J. Knudson, Maria Ferez, Pedro Alves-Peixoto, Dan A. Erkes, Carolina R. Melo-Silva, Lingjuan Tang, Christopher M. Snyder, Luis J. Sigal
Summary: This study reveals the induction and differentiation mechanisms of CD4-CTL during viral infection, showing that CD4-CTL are derived from terminal differentiation of classical Th1 cells and require sustained antigen presentation. The research demonstrates that CD4-CTL upregulate protein levels of transcription factors posttranscriptionally, suggesting further posttranscriptional regulation is necessary for CD4-CTL differentiation.
JOURNAL OF VIROLOGY
(2021)
Article
Microbiology
Carolina R. Melo-Silva, Pedro Alves-Peixoto, Natasha Heath, Lingjuan Tang, Brian Montoya, Cory J. Knudson, Colby Stotesbury, Maria Ferez, Eric Wong, Luis J. Sigal
Summary: The study found that the expression of IFNAR in NK cells and monocytes is crucial for the survival of mice infected with the specific pathogen ECTV, while its role in intrinsic IFNAR signaling is not essential for adaptive immune cell responses or directly protecting non-hematopoietic cells. This suggests that the anti-viral effects of IFN-I during viral infections are primarily restricted to cells of the innate immune system.
Article
Immunology
Javad Rasouli, Giacomo Casella, Larissa L. W. Ishikawa, Rodolfo Thome, Alexandra Boehm, Adam Ertel, Carolina R. Melo-Silva, Elisabeth R. Mari, Patrizia Porazzi, Weifeng Zhang, Dan Xiao, Luis J. Sigal, Paolo Fortina, Guang-Xian Zhang, Abdolmohamad Rostami, Bogoljub Ciric
Summary: The study reveals that IFN-beta therapy reduces GM-CSF production by Th cells indirectly through its effects on monocytes, and increases IL-10 expression in monocytes to suppress IL-1 beta secretion, thereby disrupting a proinflammatory loop involving several molecules derived from Th cells and monocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Carolina R. Melo-Silva, Cory J. Knudson, Lingjuan Tang, Samita Kafle, Lauren E. Springer, Jihae Choi, Christopher M. Snyder, Yajing Wang, Sangwon V. Kim, Luis J. Sigal
Summary: Genetically modified (GM) mice are crucial for biomedical research, but traditional methods are expensive and require specialized personnel and equipment. The use of CRISPR and i-GONAD technology has enabled successful generation of multiple GM mouse strains in C57BL/6 background. Additionally, diet enrichment and co-housing with highly fertile females improved pregnancy success and litter survival.