Structural changes in lignin during integrated process of steam explosion followed by alkaline hydrogen peroxide of Eucommia ulmoides Oliver and its effect on enzymatic hydrolysis

Eucommia ulmoides Oliver (EU) wood was successively treated by a combined system based on steam explosion pretreatment (SEP) and alkaline hydrogen peroxide post-treatment (AHPP). In this case, SEP was to disrupt the lignocellulosic structure, and the subsequent AHPP process was to isolate the high-purity lignin and cellulose-rich substrates. Results showed that the lignin fractions obtained during the AHPP exhibited smaller molecular weights, narrow polydispersity, less phenolic OH groups and lower syringyl/guaiacyl ratios (S/G) than those of the milled wood lignin (SEMWL) obtained from the only steam exploded EU. NMR characterization of lignin revealed that the AHPP process has a slight effect on the composition and molecular characteristic of lignin, and the lignin isolated had lower amounts of substructures (aryl-beta-ether, resinol, and phenylcoumaran linkages) as compared to those in SEMWL. Moreover, the subsequent SEP followed by AHPP process enhanced the enzymatic hydrolysis of cellulose-rich substrates to a maximum value of 91.69%. It was found that the synergistic treatment removed most of lignin, degraded hemicelluloses, and incurred a higher crystalline index and surface area of the cellulose-rich substrates as compared to the only steam explosion pretreatment. The combination of the SEP and AHPP processes is an environmentally benign and advantageous scheme for the production of high-purity lignin and cellulose-rich substrates, which will be further transformed into the value-added biomaterials and bioethanol. (C) 2015 Elsevier Ltd. All rights reserved.