Review
Gastroenterology & Hepatology
Tawfik Khoury, Rodica Gincul, Ismael Mohammedi, Wisam Sbeit, Bertrand Napoleon
Summary: The use of antibiotics for prophylaxis in pancreatic cystic lesions is still a debated topic. Current research lacks in quantity and has a small sample size, leading to biased results. Pooled data analysis shows that antibiotic prophylaxis does not have a significant impact on the rate of cyst infection. Larger international studies are needed to resolve this controversy.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Multidisciplinary Sciences
Shutong Zhou, Hongtao Wei, Huihong Zhai
Summary: Mediastinal masses are uncommon and difficult to diagnose. EUS-FNA is a minimally invasive technique for diagnosis, but carries the risk of infection and complications.
Article
Medicine, General & Internal
Hyoun Wook Lee, Kwang Min Kim
Summary: The study suggests that the novel EUS-FNB needle (NEFN) is an effective and reliable alternative to the conventional EUS-FNA needle (CEFN) in terms of tissue acquisition rate and quality of histologic sampling.
Article
Oncology
Maria Yanqing Chen, Neda Zarrin-Khameh, Ya Xu
Summary: This article reports a retrospective study on FNA of the pancreas, with a focus on metastatic breast cancer. The analysis of 274 cases revealed that breast cancer is the most common metastatic tumor to the pancreas.
Article
Endocrinology & Metabolism
Alessandro Gambella, Enrico Costantino Falco, Jasna Metovic, Francesca Maletta, Claudio De Angelis, Roberta Maragliano, Silvia Uccella, Donatella Pacchioni, Mauro Papotti
Summary: Pancreatic neuroendocrine tumors (PanNETs) with amyloid-rich stromal component present unique diagnostic challenges in EUS-guided fine needle aspiration cytology (EUS-FNAC). The neuroendocrine nature is confirmed by immunocytochemistry, while the amyloid stroma is identified by positive Congo red staining.
ENDOCRINE PATHOLOGY
(2021)
Review
Medicine, General & Internal
Galab M. Hassan, Louise Laporte, Sarto C. Paquin, Charles Menard, Anand Sahai, Benoit Masse, Helen Trottier
Summary: A systematic review and meta-analysis compared the diagnostic accuracy of EUS-guided FNA and FNB for pancreatic cancer. The meta-analysis showed that EUS-FNB had superior diagnostic accuracy compared to EUS-FNA for pancreatic cancer.
Article
Surgery
Jian-Han Lai, Ching-Chung Lin, Hsiang-Hung Lin, Ming-Jen Chen
Summary: This study compared the diagnostic accuracy and number of needle passes between CE-EUS-FNB and conventional EUS-FNB, showing similar diagnostic accuracy but fewer needle passes in the CE-EUS-FNB group, without additional benefit in diagnostic yield improvement.
SURGICAL ENDOSCOPY AND OTHER INTERVENTIONAL TECHNIQUES
(2022)
Article
Biochemistry & Molecular Biology
Jeeyong Lee, Hwa Jeong Ha, Da Yeon Kim, Jae Soo Koh, Eun Ju Kim
Summary: Fine needle aspiration cytology (FNAC) is a valuable tool for evaluating lymphadenopathy and diagnosing malignant tumors. However, some tumor diagnoses may still be unclear. In this study, we used mRNA sequencing technology to re-analyze misinterpreted patient samples and identified under-diagnosed characteristics of non-Hodgkin's lymphoma. Through bioinformatic and biological analysis, we identified genes and pathways potentially involved in this type of tumor. FOS, ENDOG, and PRKAR2B were identified as hub genes, and their association with lymphoma was confirmed by the cBioPortal database. These findings suggest the potential use of these candidate genes as biomarkers to improve the accuracy of FNAC diagnosis in lymphadenopathy, ultimately leading to better prognosis for patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Gastroenterology & Hepatology
Zhiwang Li, Wei Liu, Xiaoda Xu, Peiyu Li
Summary: This study compared the diagnostic value and safety of endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) and endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) in sampling solid lesions, showing that FNB has better diagnostic accuracy and tissue core rates in solid gastrointestinal lesions, with fewer needle passes, but no difference in solid pancreatic disease.
JOURNAL OF CLINICAL GASTROENTEROLOGY
(2022)
Article
Gastroenterology & Hepatology
Shintaro Nakano, Yasuyuki Kawamoto, Yoshito Komatsu, Rika Saito, Ken Ito, Takahiro Yamamura, Kazuaki Harada, Satoshi Yuki, Kazumichi Kawakubo, Ryo Sugiura, Shin Kato, Koji Hirata, Hajime Hirata, Masahito Nakajima, Ryutaro Furukawa, Yunosuke Takishin, Kousuke Nagai, Isao Yokota, Keisuke H. Ota, Shinji Nakaoka, Masaki Kuwatani, Naoya Sakamoto
Summary: This study investigated the use of endoscopic ultrasound-guided fine-needle aspiration samples for microbiome analysis in pancreatic cancer. The results showed that the bacterial composition of pancreatic cancer is different from that of the stomach and duodenum.
Review
Gastroenterology & Hepatology
Irving Levine, Arvind J. Trindade
Summary: Endoscopic ultrasound tissue acquisition, through EUS-FNA and EUS-FNB, is commonly used for pancreatic mass lesions, subepithelial lesions, and lymph node biopsy. While both methods are safe and offer high diagnostic value, there are potential limitations with EUS-FNA related to cytological aspirations. Comparative trials between EUS-FNA and EUS-FNB for pancreatic mass lesions have shown conflicting results, with the need for further large-scale studies for other types of lesions.
WORLD JOURNAL OF GASTROENTEROLOGY
(2021)
Article
Oncology
Shubo Pan, Jie Liu, Jiefang Guo, Qilin Zhu, Liangjing Wang, Xiaohua Shi
Summary: Based on a 2-center retrospective study, EUS-FNA was clinically significant in about 40% of low-risk PCLs, especially in young, large cyst size, and overweight patients.
FRONTIERS IN ONCOLOGY
(2022)
Article
Medicine, General & Internal
Hyun Seok Lee, Eaum-Seok Lee
Summary: This article reports a case of an unexpected catecholamine crisis that occurred after EUS-FNB. Despite being considered relatively safe, careful evaluation is required when performing biopsy of lesions around the aorta.
Article
Geriatrics & Gerontology
Koji Takahashi, Hiroshi Ohyama, Yuichi Takiguchi, Yu Sekine, Shodai Toyama, Nana Yamada, Chihei Sugihara, Motoyasu Kan, Mayu Ouchi, Hiroki Nagashima, Yotaro Iino, Yuko Kusakabe, Kohichiroh Okitsu, Izumi Ohno, Naoya Kato
Summary: This study investigated the safety of EUS-FNA for pancreatic solid masses in patients aged >= 80 years. The results showed that the elderly group required fewer sedation doses and had similar adverse event rates compared to the nonelderly group. There was no significant difference in the decrease of percutaneous oxygen saturation between the two groups.
GERIATRICS & GERONTOLOGY INTERNATIONAL
(2023)
Article
Medicine, General & Internal
Miruna Patricia Olar, Sorana D. Bolboaca, Cristina Pojoga, Ofelia Mosteanu, Marcel Gheorghiu, Radu Seicean, Ioana Rusu, Zeno Sparchez, Nadim Al Hajjar, Andrada Seicean
Summary: Endoscopic ultrasound fine needle aspiration (EUS-FNA) cytology is useful in the differentiation of pancreatic cysts, with higher sensitivity when the solid component is targeted. This study aimed to evaluate the diagnostic value of contrast-enhanced guided EUS-FNA (CH-EUS-FNA) in enhanced mural nodules and discrimination between different cysts. Results showed that mucinous cysts had higher arterial enhancement on contrast-enhanced endoscopic ultrasound (CH-EUS), and CH-EUS-FNA identified mucinous cysts and malignancy with high sensitivity and specificity. A comprehensive assessment of solid components using contrast-enhanced endoscopic ultrasound is important for the identification of malignant cysts.
Article
Oncology
Catherine G. Tran, Scott K. Sherman, Aaron T. Scott, Po Hien Ear, Chandrikha Chandrasekharan, Andrew M. Bellizzi, Joseph S. Dillon, Thomas M. O'Dorisio, James R. Howe
Summary: This study found that elevated levels of CgA, PST, and NKA during follow-up after surgery for SBNETs were associated with lower progression-free survival and overall survival, with PST demonstrating better discrimination of outcomes. Using PST most accurately detected disease progression during the follow-up period.
ANNALS OF SURGICAL ONCOLOGY
(2021)
Article
Pathology
Benjamin J. Van Treeck, Taofic Mounajjed, Roger K. Moreira, Mushfig Orujov, Daniela S. Allende, Andrew M. Bellizzi, Stephen M. Lagana, Jaime Davila, Erik Jessen, Rondell P. Graham
Summary: Steatohepatitic hepatocellular carcinoma presents with a unique gene expression profile distinct from conventional hepatocellular carcinoma, characterized by upregulation of the hedgehog pathway, downregulation of carnitine palmitoyltransferase 2, and a zone 3 hepatocytes phenotype.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2021)
Article
Pathology
Alexandra L. Isaacson, Ramakrishna R. Sompallae, Natalya Guseva, Andrew M. Bellizzi, Aaron D. Bossler, Deqin Ma
Summary: This study aimed to identify therapeutic targets and correlate with clinical outcomes in metastatic UM through mutation profiling using NGS. The results showed that GNAQ/11-SF3B1 mutations were associated with a longer time to first metastasis compared to GNAQ/11-BAP1 mutations. Coexisting BAP1/SF3B1 and GNAQ/11 mutations were unique to UM, and SF3B1 mutations may play a role in UM metastasis.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Oncology
Catherine G. Tran, Luis C. Borbon, Jacqueline L. Mudd, Ellen Abusada, Solmaz AghaAmiri, Sukhen C. Ghosh, Servando Hernandez Vargas, Guiying Li, Gabriella Beyer, Mary McDonough, Rachel Li, Carlos H. F. Chan, Susan A. Walsh, Thaddeus J. Wadas, Thomas O'Dorisio, M. Sue O'Dorisio, Ramaswamy Govindan, Paul F. Cliften, Ali Azhdarinia, Andrew M. Bellizzi, Ryan C. Fields, James R. Howe, Po Hien Ear
Summary: Gastroenteropancreatic neuroendocrine neoplasms (GEP NENs) are rare cancers with increasing incidence. The lack of suitable cellular and animal models for studying and testing new therapies has posed challenges for research on GEP NENs. This study successfully established two PDX models (NEC913 and NEC1452) and demonstrated their utility in drug discovery and research on GEP NENs.
Article
Hematology
Nicholas Borcherding, Kevin J. Severson, Nicholas Henderson, Luana S. Ortolan, Allison C. Rosenthal, Andrew M. Bellizzi, Vincent Liu, Brian K. Link, Aaron R. Mangold, Ali Jabbari
Summary: Cutaneous T-cell lymphomas (CTCLs) are a group of diseases caused by skin-tropic T-cell proliferation. The course of the disease varies, with some patients having indolent progression while others experience aggressive progression and poor survival. S e ' zary syndrome (SS), a leukemic variant of CTCL, lacks consistent markers for diagnosis and treatment selection. A study using single-cell sequencing identified distinct cell populations in SS and potential mechanisms of immune evasion.
Article
Biochemistry & Molecular Biology
Muhammad Furqan, Taher Abu-Hejleh, Laura M. Stephens, Stacey M. Hartwig, Sarah L. Mott, Casey F. Pulliam, Michael Petronek, John B. Henrich, Melissa A. Fath, Jon C. Houtman, Steven M. Varga, Kellie L. Bodeker, Aaron D. Bossler, Andrew M. Bellizzi, Jun Zhang, Varun Monga, Hariharasudan Mani, Marina Ivanovic, Brian J. Smith, Margaret M. Byrne, William Zeitler, Brett A. Wagner, Garry R. Buettner, Joseph J. Cullen, John M. Buatti, Douglas R. Spitz, Bryan G. Allen
Summary: The combination of pharmacological ascorbate and chemotherapy improves tumor response in advanced stage non-small cell lung cancer (NSCLC) patients and appears to alter the host immune response. Further investigation is needed to explore the potential of this treatment as an adjuvant to immunotherapy.
Article
Cell Biology
Dipti M. Karamchandani, Brian Cox, Stefano La Rosa, Andrew M. Bellizzi, Chanjuan Shi, Raul S. Gonzalez
Summary: This study aimed to assess the practice trends among pathologists worldwide when reporting digestive system neuroendocrine neoplasms. The results of an online survey showed variations in the diagnosis and management of these tumors. The survey also highlighted the need for clearer guidelines to reduce practice variations in the evaluation of neuroendocrine neoplasms.
Article
Biochemistry & Molecular Biology
Nicholas J. Caldwell, Hui Li, Andrew M. Bellizzi, Jia Luo
Summary: This study investigated the expression of mesencephalic astrocyte-derived neurotrophic factor (MANF) in chronic alcoholic pancreatitis (CAP) and chronic non-alcoholic pancreatitis (CNP). The results showed that MANF expression was higher in ducts of CAP, while it was higher in acini of CNP and normal pancreatic tissue (NPT). Furthermore, there was a positive correlation between fibrosis and MANF levels in the ducts.
Article
Multidisciplinary Sciences
Silje Kjlle, Kenneth Finne, Even Birkeland, Vandana Ardawatia, Ingeborg Winge, Sura Aziz, Gril Knutsvik, Elisabeth Wik, Joao A. Paulo, Heidrun Vethe, Dimitrios Kleftogiannis, Lars A. Akslen
Summary: This study aims to investigate the response of breast cancer cell lines to hypoxia and identify the associated protein features. The identified features can predict patient survival and have significant prognostic implications even in low-grade tumors.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Steven R. Shuken, Graeme C. McAlister, William D. Barshop, Jesse D. Canterbury, David Bergen, Jingjing Huang, Romain Huguet, Joao A. Paulo, Vlad Zabrouskov, Steven P. Gygi, Qing Yu
Summary: The combination of tandem mass tags (TMT) and tribrid mass spectrometers provides high quantitative accuracy in high-throughput proteomics. The new Orbitrap Ascend mass spectrometer improves sensitivity and speed, allowing for the identification and quantification of more proteins.
ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Jaydira Del Rivero, Kimberly Perez, Erin B. Kennedy, Erik S. Mittra, Namrata Vijayvergia, Junaid Arshad, Sandip Basu, Aman Chauhan, Arvind N. Dasari, Andrew M. Bellizzi, Alexandra Gangi, Erin Grady, James R. Howe, Jana Ivanidze, Mark Lewis, Josh Mailman, Nitya Raj, Heloisa P. Soares, Michael C. Soulen, Sarah B. White, Jennifer A. Chan, Pamela L. Kunz, Simron Singh, Thorvardur R. Halfdanarson, Jonathan R. Strosberg, Emily K. Bergsland
Summary: This article aims to provide systemic therapy recommendations for patients with well-differentiated grade 1 to grade 3 metastatic gastroenteropancreatic neuroendocrine tumors (GEP-NETs). Through a systematic review and analysis of relevant studies, the recommendations for first-line and second-line therapies for different stages and types of GEP-NETs are provided, along with qualifying statements for treatment choice. The importance of multidisciplinary team management and shared decision-making with patients is emphasized.
JOURNAL OF CLINICAL ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Kelsey L. Hickey, Sharan Swarup, Ian R. Smith, Julia C. Paoli, Enya Miguel Whelan, Joao A. Paulo, J. Wade Harper
Summary: This study provides insights into the selectivity of macroautophagy for recycling membrane-bound organelles and identifies YIPF3 and YIPF4 as receptors for Golgiphagy. The findings also highlight the importance of these receptors in Golgi remodelling during neuronal differentiation.
Article
Biochemical Research Methods
Joel S. Gygi, Xinyue. Liu, Benjamin P. Levi, Joao A. Paulo
Summary: The study investigates the impact of dysfunction in the GET pathway on protein abundance in yeast deletion strains. The findings reveal differential abundance of certain proteins in the deletion strains, some of which are membrane-associated, while the abundance of many tail-anchored proteins remains unchanged. This study provides valuable insights into the roles of GET genes and alternative pathways that maintain cellular function despite disruption of the GET pathway.
Article
Cell Biology
Utsarga Adhikary, Joao A. Paulo, Marina Godes, Shrabasti Roychoudhury, Michelle S. Prew, Yael Ben-Nun, Ellen W. Yu, Amit Budhraja, Joseph T. Opferman, Dipanjan Chowdhury, Steven P. Gygi, Loren D. Walensky
Summary: MCL-1 plays a dominant role in cancer pathogenesis and chemoresistance, but its inhibitors have toxic side effects in clinical trials. MCL-1 biology is complex, involving multiple isoforms, unresolved structure and function, and challenges in distinguishing noncanonical activities from apoptotic response. Deletion or targeting of MCL-1 induces DNA damage and inhibits cell proliferation. MCL-1 inhibitors have potential therapeutic applications as anti-proliferative drugs, but the therapeutic window concerns need to be addressed.
Article
Cell Biology
Selcan Aydin, Duy T. Pham, Tian Zhang, Gregory R. Keele, Daniel A. Skelly, Joao A. Paulo, Matthew Pankratz, Ted Choi, Steven P. Gygi, Laura G. Reinholdt, Christopher L. Baker, Gary A. Churchill, Steven C. Munger
Summary: This study comprehensively analyzed the proteome of genetically diverse mESC lines and found significant variability in protein abundance. It identified pluripotency-associated pathways that were not evident in transcriptome data from the same lines. Integration of protein abundance with transcript levels and chromatin accessibility revealed co-variation across molecular layers and identified drivers of quantitative variation in pluripotency-associated pathways.