Article
Chemistry, Multidisciplinary
Gabriele Conti, Anne Barenwaldt, Said Rabbani, Tobias Muhlethaler, Mirza Sarcevic, Xiaohua Jiang, Oliver Schwardt, Daniel Ricklin, Roland J. Pieters, Heinz Laubli, Beat Ernst
Summary: Carbohydrate-binding proteins typically have poor affinities for their natural glycan ligands due to shallow and solvent-exposed binding sites. Nature has utilized multivalency to enhance binding through multiple simultaneous interactions. In this study, oligovalent ligands for Siglec-8 were synthesized and their binding thermodynamics characterized. The multivalent ligands showed successful binding to Siglec-8 on immune cells and modulation of immune cell activation. This suggests that low oligovalent ligands can also induce a biological effect.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Cell Biology
Leigha D. Larsen, Karen Dockstader, Courtney L. Olbrich, Ian M. Cartwright, Lisa A. Spencer
Summary: This study proposes a framework for subphenotype characterization of intestinal eosinophils based on the relative intensity of surface CD11c expression. Using this framework, the researchers characterized intestinal eosinophil subphenotypes and monitored their plasticity in health and disease. Their findings provide insights into the heterogeneity of intestinal tissue eosinophils and offer a framework for measuring and tracking eosinophil subphenotype versatility in situ in health and disease.
JOURNAL OF LEUKOCYTE BIOLOGY
(2022)
Article
Immunology
Daniela J. Carroll, Yun Cao, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Siglec-8 signals through an unexpected set of signaling molecules in IL-5-primed eosinophils to induce cell death, challenging the expectation that ITIM-bearing Siglecs signal through protein tyrosine phosphatases for their downstream functions.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Shiyi Tan, Shi Chen
Summary: Silicosis is a pressing public health issue globally, with autophagy playing a crucial role in disease progression and potentially providing therapeutic targets through regulating macrophage apoptosis. Exogenous chemicals like lipopolysaccharides or natural product ingredients may have either detrimental or beneficial effects on silicosis fibrosis by modulating macrophage autophagy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Patricia da Silva Marques, Alessandra M. da Fonseca-Martins, Monique Pacheco Duarte Carneiro, Natalia R. T. Amorim, Camila R. Rodrigues de Pao, Claudio Canetti, Bruno L. Diaz, Herbert L. de Matos Guedes, Christianne Bandeira-Melo
Summary: The study found that eosinophils regulate intracellular L. amazonensis infection in macrophages through eosinophil-derived PGD2, preventing an increase in amastigote numbers. This may serve as a complementary therapeutic tool for managing cutaneous leishmaniasis caused by L. amazonensis.
CELLULAR IMMUNOLOGY
(2021)
Article
Immunology
Yun Cao, Clayton H. Rische, Bruce S. Bochner, Jeremy A. O'Sullivan
Summary: Siglec-8 is a receptor that binds to sialic acid and has different inhibitory activities on eosinophils and mast cells. Binding of Siglec-8 to antibodies induces cell death in primed eosinophils, but inhibits cellular activation in mast cells. Removal of specific sialylated ligands on cell surfaces alters the effects of Siglec-8 and induces mast cell death without cytokine priming.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Anna-Dorothea Gorki, Dorte Symmank, Sophie Zahalka, Karin Lakovits, Anastasiya Hladik, Brigitte Langer, Barbara Maurer, Veronika Sexl, Renate Kain, Sylvia Knapp
Summary: This study proposes a novel culture model for studying and expanding murine primary alveolar macrophages (AMs) in vitro over several months. The cultured AMs maintain typical features and surface markers, and exhibit similar functions and transcriptional profile to primary AMs. When transferred into AM-deficient mice, the cultured AMs efficiently engraft in the lung and perform key macrophage functions.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Immunology
Alisha Chetty, Matthew G. G. Darby, Jamie Pillaye, A'ishah Taliep, Adam F. F. Cunningham, Matthew K. K. O'Shea, Gnatoulma Katawa, Laura E. E. Layland, Manuel Ritter, William G. C. Horsnell
Summary: Helminth-induced eosinophils accumulate around the parasite after it has left the infection site. These eosinophils have a complex role in parasite control and may contribute to long-term immunopathogenesis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biology
Jiang Li, Rebecca E. Ruggiero-Ruff, Yuxin He, Xinru Qiu, Nancy Lainez, Pedro Villa, Adam Godzik, Djurdjica Coss, Meera G. Nair
Summary: The incidence of obesity is increasing worldwide, and new therapeutic approaches are urgently needed. Sex differences in immune cell activation play a role in obesity-related pathologies, with males being more susceptible to comorbidities and inflammation. This study demonstrates that the macrophage-secreted protein RELM alpha critically protects females against high-fat diet-induced obesity. The study identifies the role of the RELM alpha-macrophage-eosinophil axis in sex-specific protection against obesity and identifies new therapeutic targets for obesity.
Article
Pharmacology & Pharmacy
Xuejun Xiao, Yan Peng, Zheyue Wang, Louqian Zhang, Tingting Yang, Yangyang Sun, Yufeng Chen, Wenqing Zhang, Xinxia Chang, Wen Huang, Shuning Tian, Zhenqing Feng, Xinhua Nabi, Qi Tang, Yuan Mao
Summary: The study successfully constructed a specific antibody S15-4E6A, which demonstrated tumor-inhibitory effects on lung adenocarcinoma cells and xenografts. S15-4E6A was found to regulate macrophage polarization and may be a potential treatment strategy for S15-positive lung adenocarcinoma.
PHARMACOLOGICAL RESEARCH
(2022)
Article
Immunology
Kristina Zec, Stephanie Thiebes, Jenny Bottek, Devon Siemes, Philippa Spangenberg, Duc Viet Trieu, Nils Kirstein, Nirojah Subramaniam, Robin Christ, Diana Klein, Verena Jendrossek, Maria Loose, Florian Wagenlehner, Jadwiga Jablonska, Thilo Bracht, Barbara Sitek, Bettina Budeus, Ludger Klein-Hitpass, Dirk Theegarten, Olga Shevchuk, Daniel R. Engel
Summary: This study provides novel insights into the molecular adaptations of alveolar macrophages (AM) upon lung infection with Streptococcus pneumoniae. The results reveal a strong neutrophil-associated proteomic signature in AM, and identify CD11b as a central molecular hub influencing neutrophil recruitment, activation, and migration.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Ewelina Hoffman, Paulina Napieralska, Rhamiya Mahendran, Darragh Murnane, Victoria Hutter
Summary: This study aimed to distinguish between activated and non-activated alveolar macrophage using high content image analysis techniques, and to explore the generation process of 'foamy' macrophage phenotypes. The results showed distinct morphological and functional features of macrophages activated by LPS/IFN-gamma, as well as the proinflammatory characteristics of 'foamy' macrophages induced by certain compounds.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Calum C. Bain, Pieter A. Louwe, Nicholas J. Steers, Alberto Bravo-Blas, Lizi M. Hegarty, Clare Pridans, Simon W. F. Milling, Andrew S. MacDonald, Dominik Rueckerl, Stephen J. Jenkins
Summary: This study reveals the existence of a rare population of F4/80(lo)MHCII(+) macrophages in murine serous cavities, which are regulated by microbiota. Furthermore, the cytokine RELM-alpha plays a sex and site-specific role in regulating the survival and differentiation of these macrophages.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2022)
Article
Cell Biology
Soo Jung Cho, Alexander Pronko, Jianjun Yang, Kassandra Pagan, Heather Stout-Delgado
Summary: This study investigates the role of Ch25h in modulating innate immune responses by macrophages during Streptococcus pneumoniae infection. The findings demonstrate the important role of Ch25h in regulating the production of inflammatory cytokines and bacterial clearance in the lung.
Article
Cell Biology
Yuhong Xiao, Ye Yuan, Yuhui Yang, Bo Liu, Zhanyuan Ding, Jun Luo, Shengsong Chen, Lingling Yu
Summary: ObjectiveGTP cyclohydrolase 1 (GCH1) has been reported to protect against ferroptosis. This study aimed to investigate whether GCH1 could reduce lipopolysaccharide (LPS)-induced macrophage polarization and inflammation by inhibiting ferroptosis. Bioinformatics analysis and experimental studies were performed to elucidate the function of GCH1 in macrophages.
INFLAMMATION RESEARCH
(2023)
Article
Parasitology
Alexis Boyd, Kristin Killoran, Edward Mitre, Thomas B. Nutman
EXPERIMENTAL PARASITOLOGY
(2015)
Article
Immunology
Holly Evans, Kristin E. Killoran, Blima K. Mitre, C. Paul Morris, So-Young Kim, Edward Mitre
JOURNAL OF IMMUNOLOGY
(2015)
Article
Gastroenterology & Hepatology
Kristin E. Killoran, Amber D. Miller, Karen S. Uray, Norman W. Weisbrodt, Robia G. Pautler, Sanna M. Goyert, Nico van Rooijen, Margaret E. Conner
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2014)
Article
Immunology
Marc P. Huebner, David Larson, Marina N. Torrero, Ellen Mueller, Yinghui Shi, Kristin E. Killoran, Edward Mitre
CLINICAL IMMUNOLOGY
(2011)
Letter
Dermatology
Caroline M. Percopo, Kimberly D. Dyer, Kristin E. Killoran, Helene F. Rosenberg
EXPERIMENTAL DERMATOLOGY
(2010)
Article
Immunology
Stanislaw J. Gabryszewski, Ofir Bachar, Kimberly D. Dyer, Caroline M. Percopo, Kristin E. Killoran, Joseph B. Domachowske, Helene F. Rosenberg
JOURNAL OF IMMUNOLOGY
(2011)
Article
Immunology
Marc P. Huebner, Yinghui Shi, Marina N. Torrero, Ellen Mueller, David Larson, Kateryna Soloviova, Fabian Gondorf, Achim Hoerauf, Kristin E. Killoran, J. Thomas Stocker, Stephen J. Davies, Kristin V. Tarbell, Edward Mitre
JOURNAL OF IMMUNOLOGY
(2012)
Article
Virology
Peter Rigaux, Kristin E. Killoran, Zhijun Qiu, Helene F. Rosenberg
Article
Multidisciplinary Sciences
Holly Evans, Kristin E. Killoran, Edward Mitre
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2014)
Article
Allergy
Kristin E. Killoran, Laura E. Kropp, Alyssa R. Lindrose, Holly E. Curtis, David Cook, Edward Mitre
CLINICAL AND EXPERIMENTAL ALLERGY
(2019)
Article
Veterinary Sciences
Kristin E. Killoran, Courtney A. Walsh, Jennifer L. Asher, Molly B. Tarleton, Steven R. Wilson
Summary: In rhesus macaques, a combination of low-dose ketamine and high-dose dexmedetomidine provides smoother induction of anesthesia, significantly shorter recovery time, and a 30-minute window of anesthesia with sufficient analgesia for mild to moderately painful procedures compared to using ketamine alone. Gender does not have a significant effect on the outcomes of this combination.
JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
(2021)
Article
Veterinary Sciences
Brian J. Smith, Kristin E. Killoran, Jiajie J. Xu, Jessica D. Ayers, Lon Kendall
JOURNAL OF THE AMERICAN ASSOCIATION FOR LABORATORY ANIMAL SCIENCE
(2018)