Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 365, Issue 1-2, Pages 118-125Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2010.12.021
Keywords
Multiplexed assay; Luminex; Epitope binning; Mouse monoclonal antibody
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We describe an assay and data evaluation technique for sorting a panel of murine monoclonal antibodies according to epitope specificities. The assay analyzes the simultaneous binding (pairing) of antibodies to an antigen and groups together antibodies with similar pairing profiles. Similar profiles indicate that the antibodies bind to the same or closely related epitopes. The assay works well with crude hybridoma supernatants and can be multiplexed. These features make the assay particularly suitable for the early phase of hybridoma/antibody screening when antibodies are available only as low volume culture harvest samples. (C) 2011 Elsevier B.V. All rights reserved.
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