Journal
JOURNAL OF IMMUNOLOGICAL METHODS
Volume 344, Issue 1, Pages 58-63Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jim.2009.03.007
Keywords
Chromatin immunoprecipitation; Histone; Epigenetics; cad; TEA; Thymocyte; Rag
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Funding
- National Institute of Allergy and Infectious Diseases [R56AI070848-01A1]
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We report a streamlined procedure to efficiently carry samples from chromatin to qPCR-compatible DNA in as little as 4 h. We use this streamlined ChIP to quantify histone H3 modifications at active (cad) and repressed (T early alpha) promoters in a Rag1-deficient pro-T cell line after 1-2 h IP. We further show that the protocol readily quantified histone modifications in chromatin from 10(4) Rag-deficient DN thymocytes. Taken together, these data outline a simple, cost-effective procedure for efficient ChIP analysis. (C) 2009 Elsevier B.V. All rights reserved.
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