Article
Nanoscience & Nanotechnology
Suzanne Gillies, Rachel Verdon, Vicki Stone, David M. Brown, Theodore Henry, Lang Tran, Carl Tucker, Adriano G. Rossi, Charles R. Tyler, Helinor J. Johnston
Summary: This study used transgenic zebrafish as a model to evaluate the toxicity of nanomaterials by assessing inflammatory responses. The results showed that silver and zinc oxide nanomaterials could induce inflammatory responses in zebrafish, with silver nanomaterials having a stronger effect.
Article
Cell Biology
J. Morin-Genest, A. Saafane, D. Girard
Summary: Elimination of apoptotic neutrophils by macrophages is crucial for inflammation resolution. However, the fate and functionality of neutrophils aged in the absence of macrophages remain unclear. In this study, human neutrophils were aged in vitro and still retained the ability to generate reactive oxygen species, phagocytose, and adhere to a cell substratum. These findings suggest that a proportion of aged neutrophils may still exhibit biological functions during inflammation, when they are not efficiently eliminated by efferocytosis.
CELLULAR IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Dragana Markovic, Irina Maslovaric, Dragoslava Djikic, Vladan P. Cokic
Summary: This article explores the important role of neutrophils in MPN and the potential link between apoptosis defects and chronic inflammation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Abdelaziz Saafane, Denis Girard
Summary: This study investigated the direct effects of Fe3O4 NPs on neutrophils and found that they enhance neutrophil adhesion and phagocytosis, delay neutrophil apoptosis, and induce the production of pro-inflammatory cytokines.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Immunology
Silvie Kremserova, Anna Kocurkova, Michaela Chorvatova, Anna Klinke, Lukas Kubala
Summary: The study reveals that MPO plays a role in regulating PMN cell death by enhancing cell membrane permeabilization and phosphatidylserine surface exposure, thereby extending the lifespan of PMNs.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Ewelina Dobosz, Marta Wadowska, Marta Kaminska, Mateusz Wilamowski, Mohsen Honarpisheh, Danuta Bryzek, Jan Potempa, Jolanta Jura, Maciej Lech, Joanna Koziel
Summary: MCPIP-1 functions as a potent inhibitor of the inflammatory response by regulating apoptosis and retention of neutrophils, with its role in degrading antiapoptotic gene transcripts and interacting with specific miRNAs. This novel anti-inflammatory role of MCPIP-1 is crucial in balancing apoptosis and promoting the resolution of inflammation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Zhanyu Huang, Qingtong Zhao, Xinrong Jiang, Zejian Li
Summary: Periodontitis is an inflammatory disease characterized by the destruction of periodontal tissues, and its onset is influenced by systemic factors. The destruction of periodontal tissues is believed to be a result of unresolved inflammation. Efferocytosis, the clearance of dying cells, plays a critical role in inflammation resolution, and defective efferocytosis contributes to the persistence of chronic inflammatory diseases. This review explores the role of macrophage efferocytosis in the pathogenesis of periodontitis and highlights emerging therapeutic strategies for future periodontal treatments.
JOURNAL OF LEUKOCYTE BIOLOGY
(2023)
Article
Cell Biology
Apurwa Singhal, Shubhi Yadav, Tulika Chandra, Shrikant R. Mulay, Anil Nilkanth Gaikwad, Sachin Kumar
Summary: Neutrophil extracellular traps (NETs) are associated with various diseases and their identification and quantification methods have limitations. In this study, an imaging and computational algorithm using high content screening (HCS)-cellomics platform was developed for easy, rapid, and specific detection and analysis of NETs. The method utilized DNA dyes and automated algorithm-driven single-cell analysis to accurately detect NET-forming cells without user bias. Addition of Annexin V staining allowed differentiation between NETs, apoptosis, and necrosis. This high throughput imaging-based method provides a good platform for discovering inhibitors of NET formation and agents to modulate neutrophil death to enhance inflammation resolution.
Review
Biochemistry & Molecular Biology
Dragana C. Markovic, Irina S. Maslovaric, Marijana Kovacic, Sanja Vignjevic Petrinovic, Vesna Lj. Ilic
Summary: Myeloproliferative neoplasms (MPNs) are hematologic malignancies characterized by gene mutations and abnormal signaling pathways. Chronic inflammation is believed to play a key role in the progression of MPNs. Neutrophils in MPNs show abnormal activation and apoptotic machinery, leading to inflammation and the formation of neutrophil extracellular traps (NETs). NETs induce hematopoietic precursor proliferation in the bone marrow microenvironment, affecting hematopoietic disorders. This review discusses the potential pathophysiological significance of NET formation in MPNs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Letter
Hematology
Suhair Abdeen, Rami Abu-Fanne, Khalil Bdeir, Emad Maraga, Mohamed Higazi, Douglas B. Cines, Samuel N. Heyman, Abd Al-Roof Higazi
Summary: In patients with severe COVID-19, treatment with Tocilizumab (TCZ) led to an increase in interleukin-6 (IL-6), alpha-Defensin, and D-dimers, while colchicine reduced alpha-Defensin and D-dimer levels. This suggests that TCZ and colchicine have diverse effects on D-dimer levels in COVID-19 patients.
BRITISH JOURNAL OF HAEMATOLOGY
(2022)
Article
Engineering, Biomedical
Lais Morandini, Derek Avery, Benjamin Angeles, Paul Winston, Rebecca K. Martin, Henry J. Donahue, Rene Olivares-Navarrete
Summary: Neutrophils play a crucial role in the immune response to biomaterial implantation by releasing cytokines, chemokines, and DNA-based networks called NETs. In this study, we found that inhibiting NET formation suppresses pro-inflammatory macrophage activation and accelerates the inflammatory phase of healing, leading to greater bone formation around the implanted biomaterial. These findings highlight the importance of the neutrophil response and innate immune cell regulation during the integration of biomaterials.
ACTA BIOMATERIALIA
(2023)
Article
Cell Biology
Stefan Kuhnert, Siavash Mansouri, Michael A. Rieger, Rajkumar Savai, Edibe Avci, Gabriela Diaz-Pina, Manju Padmasekar, Mario Looso, Stefan Hadzic, Till Acker, Stephan Klatt, Jochen Wilhelm, Ingrid Fleming, Natascha Sommer, Norbert Weissmann, Claus Vogelmeier, Robert Bals, Andreas Zeiher, Stefanie Dimmeler, Werner Seeger, Soni S. Pullamsetti
Summary: This study found an increased incidence of CHIP mutations in COPD patients, which are associated with hypomethylation of PLD5, leading to elevated levels of glycerol phosphocholine, pro-inflammatory cytokines, and deteriorating lung function.
Review
Pharmacology & Pharmacy
Hamdi A. Jama, Rikeish R. Muralitharan, Chudan Xu, Joanne A. O'Donnell, Mariane Bertagnolli, Bradley R. S. Broughton, Geoffrey A. Head, Francine Z. Marques
Summary: Hypertension is a challenging disease to study due to its multifactorial nature. Various rodent models have been instrumental in uncovering the genetic, cellular, and molecular mechanisms underlying essential hypertension and testing new treatments. By analyzing these models systemically, researchers have gained insights into how different target organs and systems influence blood pressure in hypertensive conditions.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Immunology
Dylan A. McCreedy, Clare L. Abram, Yongmei Hu, Sun Won Min, Madison E. Platt, Megan A. Kirchhoff, Shelby K. Reid, Frank L. Jalufka, Clifford A. Lowell
Summary: The deficiency of Syk in neutrophils can improve long-term functional recovery after spinal cord injury, but does not promote long-term white matter sparing. Syk has an impact on neutrophil activation, cytokine expression, and cell death in the acutely injured spinal cord.
JOURNAL OF NEUROINFLAMMATION
(2021)
Review
Cardiac & Cardiovascular Systems
Gabrielle Fredman, Katherine C. MacNamara
Summary: Inflammation-resolution is a highly coordinated process governed by endogenous factors, with imbalances potentially leading to non-resolving inflammation and diseases. Recent studies emphasize the importance of balanced ratios of specialized pro-resolving mediators and pro-inflammatory lipids in timely inflammation resolution. Future research aims to address therapeutic strategies and mechanisms of disease related to these imbalances in order to tackle important human diseases such as atherosclerosis.
CARDIOVASCULAR RESEARCH
(2021)
Article
Critical Care Medicine
Ryan McGinn, Dean A. Fergusson, Duncan J. Stewart, Arnold S. Kristof, Carly C. Barron, Bernard Thebaud, Lauralyn McIntyre, Dawn Stacey, Mark Liepmann, Aurore Dodelet-Devillers, Haibo Zhang, Richard Renlund, Elliot Lilley, Gregory P. Downey, Earl G. Brown, Lucie Cote, Claudia C. Dos Santos, Alison E. Fox-Robichaud, Sabah N. A. Hussain, John G. Laffey, Mingyao Liu, Jenna MacNeil, Holly Orlando, Salman T. Qureshi, Patricia Turner, Brent W. Winston, Manoj M. Lalu
Summary: This study aimed to establish expert-guided consensus on the selection and reporting of surrogate endpoints in small animal models of acute lung injury. A total of 42 statements were evaluated, with 29 deemed important based on varying levels of evidence. Endpoints like temperature changes and body weight loss received strong support, while the importance of analgesia and transparent reporting of surrogate endpoints was emphasized.
CRITICAL CARE MEDICINE
(2021)
Article
Respiratory System
Kelly S. Schweitzer, Taylor Crue, Jordan M. Nall, Daniel Foster, Satria Sajuthi, Kelly A. Correll, Mari Nakamura, Jamie L. Everman, Gregory P. Downey, Max A. Seibold, James P. Bridges, Karina A. Serban, Hong Wei Chu, Irina Petrache
Summary: This study found that IAV can increase the expression of ACE2 and TMPRSS2, which may facilitate the infection of SARS-CoV-2 in the distal lung. IAV may also increase vulnerability to lung injury such as acute respiratory distress syndrome during viral co-infections by altering ACE2.
EUROPEAN RESPIRATORY JOURNAL
(2021)
Article
Cell Biology
Juan Manuel Belardinelli, Deepshikha Verma, Wei Li, Charlotte Avanzi, Crystal J. Wiersma, John T. Williams, Benjamin K. Johnson, Matthew Zimmerman, Nicholas Whittel, Bhanupriya Angala, Han Wang, Victoria Jones, Veronique Dartois, Vinicius C. N. de Moura, Mercedes Gonzalez-Juarrero, Camron Pearce, Alan R. Schenkel, Kenneth C. Malcolm, Jerry A. Nick, Susan A. Charman, Timothy N. C. Wells, Brendan K. Podell, Jonathan L. Vennerstrom, Diane J. Ordway, Robert B. Abramovitch, Mary Jackson
Summary: This study found that antimalarial drugs can serve as potent inhibitors of M. abscessus, offering potential for clinical treatment.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Jerry A. Nick, Rebekah M. Dedrick, Alice L. Gray, Eszter K. Vladar, Bailey E. Smith, Krista G. Freeman, Kenneth C. Malcolm, L. Elaine Epperson, Nabeeh A. Hasan, Jo Hendrix, Kimberly Callahan, Kendra Walton, Brian Vestal, Emily Wheeler, Noel M. Rysavy, Katie Poch, Silvia Caceres, Valerie K. Lovell, Katherine B. Hisert, Vinicius Calado de Moura, Delphi Chatterjee, Prithwiraj De, Natalia Weakly, Stacey L. Martiniano, David A. Lynch, Charles L. Daley, Michael Strong, Fan Jia, Graham F. Hatfull, Rebecca M. Davidson
Summary: This study describes a successful phage treatment of treatment-refractory Mycobacterium abscessus pulmonary infection in a patient with severe lung disease. The phages used in the treatment were engineered to enhance their ability to lyse M. abscessus and were specifically selected based on their effectiveness against the patient's bacterial isolate. The treatment resulted in genetic stability of the bacterial isolate and improved clinical symptoms in the patient.
Article
Multidisciplinary Sciences
Silvia M. Caceres, Linda A. Sanders, Noel M. Rysavy, Katie R. Poch, Caroline R. Jones, Kyle Pickard, Tasha E. Fingerlin, Roland A. Marcus, Kenneth C. Malcolm, Jennifer L. Taylor-Cousar, David P. Nichols, Jerry A. Nick, Matthew Strand, Milene T. Saavedra
Summary: This study found that specific genes measured from peripheral blood leukocytes can predict clinical response to inhaled antibiotic therapy in patients with cystic fibrosis. Molecular quantification of systemic inflammation may indicate subgroups of CF patients with variations in underlying inflammation and with variable clinical responses to inhaled antibiotics.
Article
Microbiology
Kenneth C. Malcolm, Emily A. Wheeler, Kara Calhoun, Patricia M. Lenhart-Pendergrass, Noel Rysavy, Katie R. Poch, Silvia M. Caceres, Milene T. Saavedra, Jerry A. Nick
Summary: This study describes a method for detecting plasma anti-M. abscessus antibodies in individuals with CF. The assay is sensitive and provides information on the immune response to NTM infections. This test could be used to identify individuals with NTM pulmonary infections and track disease progression, either alone or in conjunction with other tests.
MICROBIOLOGY SPECTRUM
(2022)
Editorial Material
Critical Care Medicine
Neomi Shah
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Article
Cell Biology
Arnav Gupta, Sarah K. Sasse, Reena Berman, Margaret A. Gruca, Robin D. Dowell, Hong Wei Chu, Gregory P. Downey, Anthony N. Gerber
Summary: This study investigates the epigenetic and transcriptional responses of small airway epithelial cells to particulate matter exposure, and identifies several transcriptional pathways that may play a role in the pathogenesis of deployment-related respiratory diseases.
PHYSIOLOGICAL GENOMICS
(2022)
Article
Immunology
Jerry A. Nick, Kenneth C. Malcolm, Emily A. Wheeler, Noel M. Rysavy, Katie Poch, Silvia Caceres, Valerie K. Lovell, Emily Armantrout, Katherine B. Hisert, Milene T. Saavedra, Kara Calhoun, Delphi Chatterjee, Ibrahim Aboellail, Stacey L. Martiniano, Fan Jia, Rebecca M. Davidson
Summary: Nontuberculous mycobacteria (NTM) is an opportunistic pathogen that affects a subset of cystic fibrosis (CF) patients, leading to increased morbidity and mortality. Current clinical detection methods rely on airway cultures, which are insensitive and unsuitable for population screening or disease monitoring. The lack of sensitive and quantitative markers hinders patient care and clinical trial design. Culture-independent markers, including bacterial products, DNA, and immune response markers, offer improved sensitivity but may not distinguish between different NTM species. These markers have the potential to enhance diagnosis and treatment monitoring when combined with traditional cultures.
Article
Microbiology
Patricia M. M. Lenhart-Pendergrass, Kenneth C. C. Malcolm, Emily Wheeler, Noel M. M. Rysavy, Katie Poch, Silvia Caceres, Kara M. M. Calhoun, Stacey L. L. Martiniano, Jerry A. A. Nick
Summary: This study investigated the immune response to Mycobacterium avium in cystic fibrosis (CF). Neutrophils in the presence of complement factors, including C3 and IgM, efficiently killed M. avium in healthy individuals, but CF patients had significantly lower killing efficiency. These findings contribute to our understanding of the immune response to M. avium and provide insights for improving diagnostic and treatment strategies.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Alma E. Ochoa, Jack H. Congel, Jodi M. Corley, William J. Janssen, Jerry A. Nick, Kenneth C. Malcolm, Katherine B. Hisert
Summary: Mycobacterium abscessus is a pathogen that can cause pulmonary infections in people with chronic airway diseases. The study found that Dectin-1 can recognize M. abscessus, but blocking it does not affect the uptake of M. abscessus by macrophages. The study also found that besides Dectin-1, other receptors can also participate in the phagocytosis of M. abscessus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Respiratory System
Gregory P. Downey
Summary: The climate crisis has a wide-ranging impact on human health, and the healthcare industry itself contributes to climate change in multiple ways. To mitigate the environmental footprint, the industry should switch to more sustainable inhalers, implement carbon offset programs, invest in research and development, and raise awareness among healthcare professionals.
CANADIAN JOURNAL OF RESPIRATORY CRITICAL CARE AND SLEEP MEDICINE
(2023)
Letter
Respiratory System
Gregory P. Downey, M. Patricia Rivera, Lynn M. Schnapp, Irina Petrache, Jesse Roman, Karen J. Collishaw
ANNALS OF THE AMERICAN THORACIC SOCIETY
(2023)
Letter
Biochemistry & Molecular Biology
Hrishikesh S. Kulkarni, Janet S. Lee, Gregory P. Downey, Gustavo Matute-Bello
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hrishikesh S. Kulkarni, Janet S. Lee, Julie A. Bastarache, Wolfgang M. Kuebler, Gregory P. Downey, Guillermo M. Albaiceta, William A. Altemeier, Antonio Artigas, Jason H. T. Bates, Carolyn S. Calfee, Charles S. Dela Cruz, Robert P. Dickson, Joshua A. Englert, Jeffrey Everitt, Michael B. Fessler, Andrew E. Gelman, Kymberly M. Gowdy, Steve D. Groshong, Susanne Herold, Robert J. Homer, Jeffrey C. Horowitz, Connie C. W. Hsia, Kiyoyasu Kurahashi, Victor E. Laubach, Mark R. Looney, Rudolf Lucas, Nilam S. Mangalmurti, Anne M. Manicone, Thomas R. Martin, Sadis Matalon, Michael A. Matthay, Daniel F. McAuley, Sharon A. McGrath-Morrow, Joseph P. Mizgerd, Stephanie A. Montgomery, Bethany B. Moore, Alexandra Noel, Carrie E. Perlman, John P. Reilly, Eric P. Schmidt, Shawn J. Skerrett, Tomeka L. Suber, Charlotte Summers, Benjamin T. Suratt, Masao Takata, Rubin Tuder, Stefan Uhlig, Martin Witzenrath, Rachel L. Zemans, Gutavo Matute-Bello
Summary: Advancements in methods and technology have led to the need for an updated definition of experimental acute lung injury (ALI). Through surveys and workshops, researchers propose that ALI presents as a multidimensional entity characterized by four domains: tissue injury, alteration of the alveolar-capillary barrier, inflammatory response, and physiologic dysfunction. They suggest measurements for each domain and divide experimental ALI into a continuum of models.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)