Review
Biotechnology & Applied Microbiology
Tae Kwang Ha, Dongil Kim, Che Lin Kim, Lise Marie Grav, Gyun Min Lee
Summary: This review summarizes the factors that influence the quality of therapeutic proteins in recombinant CHO cell culture, categorizing them into culture environments, chemical additives, and host cell proteins. Understanding these factors will facilitate the development of high yield, high-quality therapeutic protein production processes.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Immunology
Manon M. J. Cox
Summary: The insect cell expression system, proposed as the preferred biosecurity strategy for vaccine production, has limitations that need to be addressed for effective use in future pandemics.
Article
Public, Environmental & Occupational Health
Quyen T. L. Tran, Ha V. Nguyen, Huyen T. Pham, Tuan V. Mai, Quyen H. M. Nguyen, Dzung V. Le, Linh N. H. Bui, Lan T. H. Hoang, Trung Q. Hoang, Trung T. Trinh
Summary: This study evaluated the use of four ELISAs for the diagnosis of melioidosis. The combined WC/Hcp1 ELISA showed the highest sensitivity and specificity. The assay proved to be a reliable method for detecting underdiagnosed cases of melioidosis.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2022)
Article
Engineering, Biomedical
Vanessa Tanja Trossmann, Thomas Scheibel
Summary: Cell-adhesive materials are crucial for tissue engineering and regenerative medicine, especially for constructing hierarchically organized tissue structures with different cell types. Recombinant spider silk proteins offer promising scaffolds due to high biocompatibility, biodegradability, and the ability for genetic modification. A genetically modified spider silk variant, eADF4(C16)-KGD, showed cell selectivity for C2C12 mouse myoblasts out of 11 tested cell lines. Co-culturing with B50 rat neuronal cells confirmed the cell-specific adhesion of eADF4(C16)-KGD material surfaces for C2C12 mouse myoblasts.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Biochemical Research Methods
Andre Henrion, Cristian-Gabriel Arsene, Maik Liebl, Gavin O'Connor
Summary: Label-free quantification (LFQ) is evaluated as a reliable method for determining the mass fraction of host cell proteins (HCPs) in bioengineered proteins. Hemoglobin-A2 (HbA(2)) protein was used as the material, and ion intensity ratios were calibrated to quantify impurities. LFQ demonstrates universal applicability and can provide accurate results with reduced complexity.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yukako Senga, Motomichi Doi, Masayoshi Onitsuka, Shinya Honda
Summary: This study establishes an approach to detect intracellular aggregates using a fluorescent-labeled artificial protein, AF.2A1, and finds that the relative amount of intracellular aggregates is correlated with the type of additives used during cell culture. Additionally, there is a moderate correlation between the relative amount of intracellular aggregates and that of extracellular aggregates, suggesting the secretion of intracellular aggregates.
CELL CHEMICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Yesenia Latorre, Mauro Torres, Mauricio Vergara, Julio Berrios, Maria Molina Sampayo, Natasha Goedecke, Dagmar Wirth, Hansjoerg Hauser, Alan J. Dickson, Claudia Altamirano
Summary: Improving the cellular capacity of CHO cells to produce therapeutic proteins is a major challenge in the biopharmaceutical industry. Overexpression of MYC and XBP1s transcription factors in CHO cells increases cell density and protein production, leading to enhanced overall performance. This study demonstrates the potential of TF-based cell engineering and process optimization for improving CHO cell productivity.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Luting Yu, Liang Li, Junli Liu, Hao Sun, Xiang Li, Hanyu Xiao, Martin Omondi Alfred, Min Wang, Xuri Wu, Yan Gao, Chen Luo
Summary: Progressive loss and dysfunction of islet beta-cells in diabetes remains a challenge in treatment. This study demonstrated that recombinant Reg3 alpha protein (rReg3 alpha) protected against beta-cell death and promoted regeneration, potentially serving as a drug for beta-cell maintenance in anti-diabetic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Philip Dettinger, Tobias Kull, Geethika Arekatla, Nouraiz Ahmed, Yang Zhang, Florin Schneiter, Arne Wehling, Daniel Schirmacher, Shunsuke Kawamura, Dirk Loeffler, Timm Schroeder
Summary: Liquid handling robots have great potential for automation in life sciences. However, their widespread use in academic settings is limited by funding, space, and maintenance specialists. In this study, the authors introduce an inexpensive and open-source personal liquid handling robot called PHIL. It is designed for inexperienced users and can be self-produced using cheap commercial and 3D-printable components. PHIL successfully automates various experiments, enabling users without programming skills to easily automate their experiments.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Kyle J. Isaacson, Brian R. Van Devener, Douglas B. Steinhauff, M. Martin Jensen, Joseph Cappello, Hamidreza Ghandehari
Summary: This study demonstrates the use of temperature-ramp liquid-cell transmission electron microscopy (LCTEM) to evaluate the solution and interfacial behavior of various polymers during thermal transitions. The results show that the solubility and behavior of polymers change with temperature, and different polymers exhibit different thermal properties.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Food Science & Technology
B. Borras-Vallverdu, A. J. Ramos, S. Martin, V. Sanchis, J. J. Rodriguez-Bencomo
Summary: A fast, simple, reliable, and inexpensive method for total DON quantification in bacterial culture assays is presented. This method can be used in screenings for DON-biodegrading microorganisms without requiring metabolite analysis.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Computer Science, Interdisciplinary Applications
P. Perez, J. A. Serrano, M. E. Martin, P. Daza, G. Huertas, A. Yufera
Summary: A computer program is developed for the system design of biosensors applied to monitoring cell culture dynamics. The program allows for the confident obtaining of system information through electrical stimulation, accurately predicting the output frequency and amplitude ranges of the voltage response. Deep insight information on cell size, number, and time-division can be extracted from comparing with real cell culture assays in the future.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Weifeng Li, Zhenlin Fan, Yan Lin, Tian-Yun Wang
Summary: This review highlights the importance of serum-free medium (SFM) in the production of recombinant therapeutic proteins (RTPs) using Chinese hamster ovary (CHO) cells, with nearly 70% of RTPs currently produced in this manner. It discusses the history, key components, preparation, and experimental design of SFM, as well as the potential benefits of small molecule compound additives in improving RTP yield and quality. Lastly, it explores the future prospects of using SFM with CHO cells for RTP production.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
John Dubczak, Nicola Reid, Masakazu Tsuchiya
Summary: This study examined various alternative methods for bacterial endotoxin detection, comparing compendial LAL methods with recombinant Factor C (rFC) reagents containing one of the three enzymes in the horseshoe crab clotting cascade. A statistically analysis showed non-inferiority between the two compendial LAL methods, but not with any of the recombinant reagents. The link between the BET and recombinant alternatives remains unresolved and requires continued caution and testing.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Polymer Science
Chavee Laomeephol, Apichai Vasuratna, Juthamas Ratanavaraporn, Sorada Kanokpanont, Jittima Amie Luckanagul, Martin Humenik, Thomas Scheibel, Siriporn Damrongsakkul
Summary: Binary-blended hydrogels fabricated from Bombyx mori silk fibroin and recombinant spider silk protein showed improved gelation time and cellular interactions compared to individual materials, suggesting potential for biomedical applications. Mixing the two materials enhanced cellular activities, making the hydrogels suitable for various biomedical uses.
Review
Allergy
Jon Genuneit, Sashini Jayasinghe, Carmen Riggioni, Rachel Louise Peters, Derek K. Chu, Daniel Munblit, Robert J. Boyle, George Du Toit, Isabel Skypala, Alexandra F. Santos
Summary: The European Academy of Allergy and Clinical Immunology is updating guidelines on food allergy diagnosis and management by conducting a new systematic review to assess the accuracy of index tests for IgE-mediated food allergy. The protocol aims to evaluate various tests' accuracy in diagnosing food allergy, which can be beneficial in both clinical and research settings.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2022)
Letter
Allergy
Misbah Noshela Ghazanfar, Ewa Anna Bartko, Nicolai Skovbjerg Arildsen, Lars K. Poulsen, Bettina Margrethe Jensen, Christian Enevold, Jesper Gronlund Holm, Anders Woetmann, Niels odum, Simon Francis Thomsen
CLINICAL AND EXPERIMENTAL ALLERGY
(2022)
Article
Biochemistry & Molecular Biology
Katrine T. Callesen, Sofia Mogren, Frida Berlin, Cecilia Andersson, Susanne Schmidt, Lotte Klitfod, Vanesa Esteban, Lars K. Poulsen, Bettina M. Jensen
Summary: This study characterized mast cells (MCs) from human saphenous veins, revealing that vein MCs mainly reside in the tunica adventitia, consist of both MCT and MCTC phenotypes, contain high levels of histamine and tryptase, and express various receptors, suggesting their potential to regulate surrounding tissues.
Letter
Allergy
Lars H. Blom, Ewa A. Bartko, Thea K. R. Moller, Lars K. Poulsen, Bettina M. Jensen
Article
Allergy
Antonella Muraro, Debra de Silva, Susanne Halken, Margitta Worm, Ekaterina Khaleva, Stefania Arasi, Audrey Dunn-Galvin, Bright Nwaru, Nicolette W. De Jong, Pablo Rodriguez Del Rio, Paul J. Turner, Pete Smith, Philippe Begin, Elizabeth Angier, Hasan Arshad, Barbara Ballmer-Weber, Kirsten Beyer, Carsten Bindslev-Jensen, Antonella Cianferoni, Celine Demoulin, Antoine Deschildre, Motohiro Ebisawa, Maria Montserrat Fernandez-Rivas, Alessandro Fiocchi, Bertine Flokstra-de Blok, Jennifer Gerdts, Josefine Gradman, Kate Grimshaw, Carla Jones, Susanne Lau, Richard Loh, Montserrat Alvaro Lozano, Mika Makela, Mary Jane Marchisotto, Rosan Meyer, Clare Mills, Caroline Nilsson, Anna Nowak-Wegrzyn, Ulugbek Nurmatov, Giovanni Pajno, Marcia Podesta, Lars K. Poulsen, Hugh A. Sampson, Angel Sanchez, Sabine Schnadt, Hania Szajewska, Ronald Van Ree, Carina Venter, Berber Vlieg-Boerstra, Amena Warner, Gary Wong, Robert Wood, Torsten Zuberbier, Graham Roberts
Summary: This article presents guidelines from the Global Allergy and Asthma European Network (GA(2)LEN) for managing food allergy. The guidelines were developed by an international task force using the latest evidence and evaluation methods, and include recommendations such as avoiding allergens, using hypoallergenic formulas, and oral immunotherapy. However, there are still gaps in evidence regarding the safety and effectiveness of these strategies.
WORLD ALLERGY ORGANIZATION JOURNAL
(2022)
Letter
Allergy
Roselie Achten, Judith Thijs, Chantal van Luijk, Edward Knol, Eveline Delemarre, Marlies de Graaf, Daphne Bakker, Joke de Boer, Femke van Wijk, Marjolein de Bruin-Weller
CLINICAL AND EXPERIMENTAL ALLERGY
(2023)
Letter
Allergy
Ewa A. A. Bartko, Jesper Elberling, Lars K. K. Poulsen, Bettina M. M. Jensen
CLINICAL AND EXPERIMENTAL ALLERGY
(2023)
Article
Clinical Neurology
F. E. V. Scheijmans, I. Cuppen, M. M. Zwartkruis, I. Signoria, C. van Ekris, F. Asselman, R. I. Wadman, E. F. Knol, W. L. van der Pol, E. J. N. Groen
Summary: Spinal muscular atrophy (SMA) is a progressive motor neuron disease that affects infants and young children. Recent therapeutic advances have improved survival and motor function in SMA patients. The most commonly used treatment, nusinersen, has been shown to be safe, but long-term effects are still unknown.
EUROPEAN JOURNAL OF PAEDIATRIC NEUROLOGY
(2023)
Letter
Allergy
Victor Lendal, Sara Fransson, Holger Mosbech, Jonas Bredtoft Boel, Natasha Kahlhofen, Lars H. Blom, Lars K. Poulsen, Lene H. Garvey
CLINICAL AND EXPERIMENTAL ALLERGY
(2023)
Letter
Allergy
Sara Fransson, Jonas Bredtoft Boel, Anne Holm, Natasha Kahlhofen, Janni Hjortlund, Holger F. Mosbech, Lars K. Poulsen, Susanne Reventlow, Lene H. Garvey
CLINICAL AND EXPERIMENTAL ALLERGY
(2023)
Article
Allergy
Sara Fransson, Christina Monnerup Damving, Jonas Bredtoft Boel, Magnus Arpi, Ida Gjorup Skinhoj, Jens Otto Jarlov, Holger F. Mosbech, Lars K. Poulsen, Lene H. Garvey
Summary: This study investigated the proportion of patients with penicillin allergy labels in a Danish hospital and classified them into no risk, low risk, and high-risk categories based on guidelines. The results showed that 10% of inpatients had a penicillin allergy label, and 17% of them could potentially have their label removed without allergy testing.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2023)
Article
Allergy
Stephanie Dramburg, Christiane Hilger, Alexandra F. Santos, Leticia de las Vecillas, Rob C. Aalberse, Nathalie Acevedo, Lorenz Aglas, Friedrich Altmann, Karla L. Arruda, Riccardo Asero, Barbara Ballmer-Weber, Domingo Barber, Kirsten Beyer, Tilo Biedermann, Maria Beatrice Bilo, Simon Blank, Philipp P. Bosshard, Heimo Breiteneder, Helen A. Brough, Merima Bublin, Dianne Campbell, Luis Caraballo, Jean Christoph Caubet, Giorgio Celi, Martin D. Chapman, Maksymilian Chruszcz, Adnan Custovic, Rebecca Czolk, Janet Davies, Nikolaos Douladiris, Bernadette Eberlein, Motohiro Ebisawa, Anna Ehlers, Philippe Eigenmann, Gabriele Gadermaier, Mattia Giovannini, Francisca Gomez, Rebecca Grohman, Carole Guillet, Christine Hafner, Robert G. Hamilton, Michael Hauser, Thomas Hawranek, Hans Juergen Hoffmann, Thomas Holzhauser, Tomona Iizuka, Alain Jacquet, Thilo Jakob, Bente Janssen-Weets, Uta Jappe, Marek Jutel, Tanja Kalic, Sandip Kamath, Sabine Kespohl, Joerg Kleine-Tebbe, Edward Knol, Andre Knulst, Jon R. Konradsen, Peter Korosec, Annette Kuehn, Gideon Lack, Thuy-My Le, Andreas Lopata, Olga Luengo, Mika Maekelae, Alessandro Maria Marra, Clare Mills, Martine Morisset, Antonella Muraro, Anna Nowak-Wegrzyn, Roni Nugraha, Markus Ollert, Kati Palosuo, Elide Anna Pastorello, Sarita Ulhas Patil, Thomas Platts-Mills, Anna Pomes, Pascal Poncet, Ekaterina Potapova, Lars K. Poulsen, Christian Radauer, Suzana Radulovic, Monika Raulf, Pierre Rouge, Joaquin Sastre, Sakura Sato, Enrico Scala, Johannes M. Schmid, Peter Schmid-Grendelmeier, Denise Schrama, Helene Senechal, Claudia Traidl-Hoffmann, Marcela Valverde-Monge, Marianne van Hage, Ronald van Ree, Kitty Verhoeckx, Stefan Vieths, Magnus Wickman, Josefina Zakzuk, Paolo M. Matricardi, Karin Hoffmann-Sommergruber
Summary: Since the discovery of IgE as a mediator of allergic diseases, our knowledge about its immunological mechanisms has greatly increased. Innovations in bioscience and technology have facilitated the production of well-defined molecules for component-resolved diagnosis, allowing personalized management of allergic diseases. The Molecular Allergology User's Guide 2.0 provides updated information on allergen molecules, their clinical relevance, and diagnostic algorithms, serving as a valuable resource for clinicians and scientists.
PEDIATRIC ALLERGY AND IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ewa A. Bartko, Lars H. Blom, Jesper Elberling, Lars K. Poulsen, Bettina M. Jensen
Summary: Recruitment to the local tissue and alerted phenotype are the hallmarks of basophils in chronic urticaria (CU). In this study, the basophil homing potential and receptor profile of CU were investigated. It was found that unstimulated basophils in CU showed higher expression of skin-associated and scavenger receptors, and lower expression of the lung-associated receptor compared to healthy basophils. IgE-mediated activation increased the expression of certain receptors and amplified the skin-homing potential of CU basophils.
Article
Allergy
Alexandra F. Santos, Carmen Riggioni, Ioana Agache, Cezmi A. Akdis, Mubeccel Akdis, Alberto Alvarez-Perea, Montserrat Alvaro-Lozano, Barbara Ballmer-Weber, Simona Barni, Kirsten Beyer, Carsten Bindslev-Jensen, Helen A. Brough, Betul Buyuktiryaki, Derek Chu, Stefano Del Giacco, Audrey Dunn-Galvin, Bernadette Eberlein, Motohiro Ebisawa, Philippe Eigenmann, Thomas Eiwegger, Mary Feeney, Montserrat Fernandez-Rivas, Helen R. Fisher, David M. Fleischer, Mattia Giovannini, Claudia Gray, Karin Hoffmann-Sommergruber, Susanne Halken, Jonathan O'B. Hourihane, Christina J. Jones, Marek Jutel, Edward Knol, George N. Konstantinou, Gideon Lack, Susanne Lau, Andreina Marques Mejias, Mary Jane Marchisotto, Rosan Meyer, Charlotte G. Mortz, Beatriz Moya, Antonella Muraro, Caroline Nilsson, Lucila Camargo Lopes de Oliveira, Liam O'Mahony, Nikolaos G. Papadopoulos, Kirsten Perrett, Rachel L. Peters, Marcia Podesta, Lars K. Poulsen, Graham Roberts, Hugh A. Sampson, Juergen Schwarze, Peter Smith, Elizabeth Huiwen Tham, Eva Untersmayr, Ronald Van Ree, Carina Venter, Brian P. Vickery, Berber Vlieg-Boerstra, Thomas Werfel, Margitta Worm, George Du Toit, Isabel Skypala
Summary: This guideline from the European Academy of Allergy and Clinical Immunology provides recommendations for diagnosing IgE-mediated food allergy, including clinical history, tests for determining IgE sensitization, and the use of oral food challenges for confirmation.
Article
Allergy
Lars H. Blom, Lasse B. Schmidt, Claus R. Johnsen, Jesper Elberling, Lars K. Poulsen, Lene H. Garvey
