Journal
JOURNAL OF IMMUNOASSAY & IMMUNOCHEMISTRY
Volume 32, Issue 1, Pages 57-69Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15321819.2010.538109
Keywords
atorvastatin; ELISA; pharmacokinetic studies; plasma; polyclonal antibody; therapeutic monitoring
Funding
- King Abdulaziz City for Science and Technology [KACS-98-16-AT]
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For the first time, a polyclonal antibody with high affinity to atorvastatin (ATR) was generated. The high specificity of the antibody for ATR among its structural analogues and co-administered therapeutic agents was proved. The antibody was employed in the development of enzyme-linked immunosorbent assay for quantitation of ATR in plasma. The assay was validated over a working range of 0.2-5 ng/ mL. The intra- and interassay precisions were satisfactory; the coefficients of variations were <= 5%. The accuracy of the method was proved as the mean recovery was 96.4 +/- 4.3%. The assay can be used in therapeutic monitoring and pharmacokinetic studies for ATR.
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