Article
Biochemistry & Molecular Biology
Bryan Le, Kan Zhu, Chelsea Brown, Brian Reid, Amin Cressman, Min Zhao, Fernando A. Fierro
Summary: Corneal wound healing is a complex biological process. Reduced sialylated glycoforms enhance electrotaxis and re-epithelialization, potentially opening new avenues to promote corneal wound healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Anastasiya S. Sokolova, Olga I. Yarovaya, Oleg I. Artyushin, Elena V. Sharova, Dmitriy S. Baev, Ekaterina D. Mordvinova, Dmitriy N. Shcherbakov, Tatiana A. Shnaider, Tatiana V. Nikitina, Iana L. Esaulkova, Polina A. Ilyina, Vladimir V. Zarubaev, Valery K. Brel, Tatyana G. Tolstikova, Nariman F. Salakhutdinov
Summary: A new series of heterocyclic derivatives with a 1,7,7-trimethylbicyclo[2.2.1]heptane fragment were designed, synthesised and biologically evaluated. These derivatives showed antiviral activity against Marburg and Ebola pseudotype viruses, and their activities were closely related to their lysosomotropic effect.
ARCHIV DER PHARMAZIE
(2023)
Article
Ophthalmology
Yun Zhang, Jin-Miao Li, Rong Lu, Zhao Liu, Xin Chen, Cintia S. de Paiva, Stephen C. Pflugfelder, De-Quan Li
Summary: This study demonstrates that the anti-inflammatory cytokine IL-37 plays a novel role in preventing corneal epithelial barrier disruption in dry eye disease by suppressing TNF-alpha and CTSS expression.
Article
Microbiology
Elias Myrvoll Lorentzen, Stian Henriksen, Christine Hanssen Rinaldo
Summary: Most humans have a lifelong BK Polyomavirus (BKPyV) infection in their urinary tract, which can cause kidney damage in kidney transplant recipients. The virus primarily enters the cells through the apical membrane and is released from the apical compartment. The virus can also be transmitted to uninfected cells through shed decoy-like cells. In kidney transplant recipients, the replication of BKPyV in the allograft can lead to allograft damage and failure.
Article
Gastroenterology & Hepatology
Banglao Xu, Xianan Wu, Yi Gong, Ju Cao
Summary: IL-27 can be involved in host immunity against Clostridioides difficile infection by induction of LL-37/CRAMP. Therefore, the IL-27-LL-37 axis may be a valuable pathway in the development of immune-based therapy.
Article
Biotechnology & Applied Microbiology
Ezgi Ulkuseven, David J. McCanna, Lakshman N. Subbaraman, Lyndon W. Jones
Summary: Antimicrobial peptides are a class of polypeptides composed of less than 100 amino acids, demonstrating strong activity against infectious bacteria. Among the peptides studied, polymyxin E and nisin showed potential for treating ocular infections with low toxicity to human corneal epithelial cells.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Infectious Diseases
Kimberly M. Brothers, Stephen A. K. Harvey, Robert M. Q. Shanks
Summary: This study investigates the impact of Serratia marcescens on corneal epithelial cell host responses, as well as the role of a bacterial transcription factor EepR in regulating host-pathogen interactions. Results show increased expression of proinflammatory and lipid signaling molecules in wild-type bacterial secretome-treated corneal cells.
Article
Pediatrics
Malik Aydin, Sebastian Schellhorn, Stefan Wirth, Wenli Zhang, Anja Ehrhardt
Summary: Research on adenovirus (Ad) is increasingly important in virotherapy, vaccine development, tumors, and gene therapy. Characterization of rare Ad types 70, 73, and 74 is necessary, as they show interestingly low transduction rates in primary cells. Further investigation into the development of diagnostic and therapeutic concepts for these rare Ad types is crucial to prevent and treat opportunistic infections in susceptible patients, especially immunocompromised individuals.
Article
Biochemistry & Molecular Biology
Hussein Al-Mughaid, Shorooq Nawasreh, Heba Naser, Younis Jaradat, Raed M. Al-Zoubi
Summary: In this study, two families (A, B) of mannose-triazole conjugates were synthesized by Cu(I) catalyzed azide-alkyne cycloaddition reaction. Family A exhibited higher activity compared to family B in a hemagglutination inhibition assay. The representative ligand 7a showed the highest inhibitory activity against FimH fimbrial lectin.
CARBOHYDRATE RESEARCH
(2022)
Article
Ophthalmology
Mona Bhargava, Surajit Sen, Varsha Bhambhani, Raj Shekhar Paul, Chandana Dutta
Summary: This retrospective observational study describes the clinical course, characteristics, and outcome of reticular epithelial corneal edema (RECE) as an adverse effect of Rho-kinase inhibitors (ROCK-I) - netarsudil (0.02%) and ripasudil (0.4%). The study found that RECE occurs with both netarsudil and ripasudil use, though with different characteristics. Corneal edema and endothelial decompensation seem to be risk factors for RECE, indicating cautious use in these patients. Further large-scale studies are needed to determine the incidence, pathophysiology, and long-term consequences of this side effect.
INDIAN JOURNAL OF OPHTHALMOLOGY
(2022)
Article
Chemistry, Organic
Emil Johansson, Remi Caraballo, Mikael Elofsson
Summary: This paper describes the synthesis of 4-O-alkyl analogues of N-acetylneuraminic acid (Neu5Ac) and the scope of the reaction. It demonstrates that activated alkyl halides and sulfonates, as well as primary alkyl iodides, can give products in useful yields. The utility of the methodology is exemplified using a specific example of synthesizing a 4-O-alkyl DANA analogue. These results expand the toolbox of Neu5Ac chemistry and have value in drug discovery and the study of Neu5Ac lectins biology.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
B. David Persson, Lijo John, Karim Rafie, Michael Strebl, Lars Frangsmyr, Monika Z. Ballmann, Katja Mindler, Menzo Havenga, Angelique Lemckert, Thilo Stehle, Lars-Anders Carlson, Niklas Arnberg
Summary: This study reveals a noncanonical entry mechanism used by human adenoviruses, where CD46 interacts directly with the hexon protein to infect host cells. CD46 is identified as an important receptor for a large group of adenoviruses, inhibiting infection by 16 out of 17 investigated HAdV-D types.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
K. Rafie, A. Lenman, J. Fuchs, A. Rajan, N. Arnberg, L-A Carlson
Summary: HAdV-F40 and F41 are major causes of diarrhea and diarrhea-related mortality in young children worldwide, with a structure that differs significantly from non-enteric HAdVs, providing new insights into HAdV architecture.
Article
Biochemistry & Molecular Biology
Charlotta Sundin, Michael Saleeb, Sara Spjut, Liena Qin, Mikael Elofsson
Summary: The study identified small molecule binders of PcrV through surface plasmon resonance screening, with subsequent analysis showing their protective effects on macrophages in a P. aeruginosa cell-based infection assay. Treatment of P. aeruginosa infections is challenging due to resistance factors and virulence systems, making virulence blocking molecules targeting PcrV valuable for development of next generation antibacterials.
Article
Chemistry, Multidisciplinary
Zahra El-Schich, Yuecheng Zhang, Tommy Goransson, Nishtman Dizeyi, Jenny L. Persson, Emil Johansson, Remi Caraballo, Mikael Elofsson, Sudhirkumar Shinde, Borje Sellergren, Anette Gjorloff Wingren
Summary: Sialylations are crucial post-translational modifications in cellular events, especially in tumor cells where high levels of sialic acid (SA) expression are associated with increased invasive potential. The use of SA-MIPs as nanoprobes shows promise in detection and analysis of breast cancer cells.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Emil Johansson, Remi Caraballo, Daniel L. Hurdiss, Nitesh Mistry, C. David Andersson, Rebecca F. Thompson, Neil A. Ranson, Georg Zocher, Thilo Stehle, Niklas Arnberg, Mikael Elofsson
Summary: CVA24v is the primary causative agent of acute hemorrhagic conjunctivitis, and there are currently no specific antiviral agents or vaccines available for combating this disease. The virus utilizes Neu5Ac-containing glycans as attachment receptors to infect host cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Organic
Emil Johansson, Remi Caraballo, Mikael Elofsson
Summary: This paper describes the synthesis of 4-O-alkyl analogues of N-acetylneuraminic acid (Neu5Ac) and the scope of the reaction. It demonstrates that activated alkyl halides and sulfonates, as well as primary alkyl iodides, can give products in useful yields. The utility of the methodology is exemplified using a specific example of synthesizing a 4-O-alkyl DANA analogue. These results expand the toolbox of Neu5Ac chemistry and have value in drug discovery and the study of Neu5Ac lectins biology.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Virology
Monika Z. Ballmann, Svjetlana Raus, Ruben Engelhart, Gyozo L. Kajan, Abdelaziz Beqqali, Patrick W. F. Hadoke, Chantal van der Zalm, Tibor Papp, Lijo John, Selina Khan, Satish Boedhoe, Katarina Danskog, Lars Frangsmyr, Jerome Custers, Wilfried A. M. Bakker, Hilde M. van der Schaar, Niklas Arnberg, Angelique A. C. Lemckert, Menzo Havenga, Andrew H. Baker
Summary: The study introduces a chimeric human adenovirus, HAdV-20-42-42, which shows promise in gene therapy and vaccine development. It is capable of stable propagation, transducing cardiovascular cells effectively, and inducing potent T-cell responses. This novel vector expands the toolbox for vaccine development, providing valuable additions to the field.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
A. Manuel Liaci, Barbara Steigenberger, Paulo Cesar Telles de Souza, Sem Tamara, Mariska Grollers-Mulderij, Patrick Ogrissek, Siewert J. Marrink, Richard A. Scheltema, Friedrich Forster
Summary: Human SPC has two functional paralogs with distinct proteolytic subunits, and its structure plays a key role in the specificity for SPs based on the length of their hydrophobic segments.
Article
Chemistry, Multidisciplinary
Emil Johansson, Remi Caraballo, Georg Zocher, Nitesh Mistry, Niklas Arnberg, Thilo Stehle, Mikael Elofsson
Summary: This study reports on the structure-based design of three classes of divalent Neu5Ac conjugates and their effect on CVA24v transduction. The results show that the divalent conjugates are more efficient than the previously reported pentavalent conjugates.
Article
Biotechnology & Applied Microbiology
Maria Benko, Koki Aoki, Niklas Arnberg, Andrew J. Davison, Marcela Echavarria, Michael Hess, Morris S. Jones, Gyozo L. Kajan, Adriana E. Kajon, Suresh K. Mittal, Iva I. Podgorski, Carmen San Martin, Goran Wadell, Hidemi Watanabe, Balazs Harrach
Summary: The family Adenoviridae consists of non-enveloped viruses with linear dsDNA genomes and medium-sized icosahedral capsids. Adenoviruses have been found in vertebrates from fish to humans. The family is divided into six genera, each of which is more prevalent in certain animal groups. The outcome of infection can range from subclinical to lethal disease.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Oncology
Sarah Beyer, Martha Kimani, Yuecheng Zhang, Alejandra Verhassel, Louise Sternbaek, Tianyan Wang, Jenny L. Persson, Pirkko Harkonen, Emil Johansson, Remi Caraballo, Mikael Elofsson, Kornelia Gawlitza, Knut Rurack, Lars Ohlsson, Zahra El-Schich, Anette Gjorloff Wingren, Maria M. Stollenwerk
Summary: This study used molecularly imprinted polymers as a novel tool to analyze the expression of sialic acid, a biomarker on cancer cells. The synthesized sialic acid imprinted polymer particles showed improved binding ability to cancer cells and demonstrated specificity in inhibiting binding with sialic acid conjugates. The results suggest that these particles could be a powerful tool in diagnosing cancer cells.
Article
Virology
Jack R. Hemsath, A. Manuel Liaci, Jeffrey D. Rubin, Brian J. Parrett, Shao-Chia Lu, Tien Nguyen, Mallory A. Turner, Christopher Y. Chen, Karolina Cupelli, Vijay S. Reddy, Thilo Stehle, M. Kathryn Liszewski, John P. Atkinson, Michael A. Barry
Summary: This study investigates the usage of CD46 receptor by Ad26 virus in different administration routes and its impact on infection efficiency. The results show that Ad26 can use CD46 as a receptor but its usage varies depending on the administration route. Ectopic expression of CD46 on cells enhances Ad26 infection, while tumor injection or intravenous injection shows lower infection efficiency. High-dose intravenous injection of Ad26 induces liver enzyme release and reduces white blood cell counts but does not cause thrombocytopenia.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
A. Manuel Liaci, Friedrich Forster
Summary: Cleavable and non-cleavable signal sequences target a quarter of the human proteome to the ER. Interaction between these signal sequences and ER targeting machinery determines protein location and translocation fidelity, as discussed in recent insights from structural biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Organic
Faiza Diaba, Gisela Trenchs
Summary: The first violet light-mediated synthesis of gamma- and delta-lactams from N-alkenyl trichloroacetamides is reported in this paper. The reactions are conducted in tetrahydrofuran or 2-methyltetrahydrofuran as the sole solvent without catalysts or additives, under non-anhydrous conditions in an air atmosphere where the solvent serves as the radical initiator.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Feroze Hussain, Sajjad Ahmed, Ashiq Hussain Padder, Qazi Naveed Ahmed
Summary: This study reports a novel and efficient one-pot synthesis method for mixed phosphorotrithioates, which does not require supplementary additives and shows broad applicability.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Hyunjin Oh, Ikyon Kim
Summary: A new 1,2,4-triazole-pyrrolo[1,2-a]pyrazine hybrid system, 6-acylpyrrolo[1,2-a][1,2,4]triazolo[5,1-c]pyrazine, was synthesized using a catalyst-free method. This method involved sequential exposure of pyrrole-2-carbonitrile-derived substrates to DMF-DMA and acyl hydrazide, resulting in the formation of acylated pyrazine and 1,2,4-triazole rings, enabling the installation of various substituents at specific positions on the core skeleton.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Ming Yan, Si-fan Wang, Yong-po Zhang, Jin-zhong Zhao, Zhuo Tang, Guang-xun Li
Summary: Here we developed an efficient photocatalytic approach for the convenient preparation of sulfinamides. The reaction allows for the gram-scale preparation of sulfinamides and the one-pot synthesis of various sulfonyl amides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Farzaneh Bandehali-Naeini, Zahra Tanbakouchian, Noushin Farajinia-Lehi, Nicolas Mayer, Morteza Shiri, Martin Breugst
Summary: Two tandem catalytic systems were developed for the synthesis of novel 3,4-disubstituted maleimides using the same Ugi adducts. Different maleimide structures can be synthesized using either Pd or Cu catalysis.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Tanya Raghava, Anjan Chattopadhyay, Subhadeep Banerjee, Nivedita Sarkar
Summary: Amine substitution of two ortho fluorine atoms of tetrafluoroterephthalonitrile through SNAr chemistry is easily achievable. But further fluorine substitution is only possible under forcing conditions, yielding valuable fluorophores for bioimaging.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Review
Chemistry, Organic
Anju Chadha, Santosh Kumar Padhi, Selvaraj Stella, Sowmyalakshmi Venkataraman, Thangavelu Saravanan
Summary: Alcohol dehydrogenases are enzymes that use cofactors for oxidation or reduction reactions of alcohols or carbonyl compounds. They are utilized in green chemistry and have applications in the production of pharmaceuticals. Recombinant enzymes have solved the challenge of producing purified enzymes in large quantities. Engineered alcohol dehydrogenases have been used in asymmetric synthesis in industry. Various methods have been established for regenerating expensive cofactors to make the enzymatic process more efficient and economically viable.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)