Article
Chemistry, Medicinal
Kseniya N. Sedenkova, Victoria I. Uvarova, Anna A. Nazarova, Aleksandra V. Peisikova, Evgeny V. Khvatov, Maksim V. Sukhorukov, Vasilisa S. Frolenko, Alexander S. Goryashchenko, Ivan S. Kholodilov, Yuri K. Grishin, Viktor B. Rybakov, Marat T. Makenov, Evgeny S. Morozkin, Lyudmila S. Karan, Liubov I. Kozlovskaya, Aydar A. Ishmukhametov, Dmitry I. Osolodkin, Elena B. Averina
Summary: A series of tetrahydroquinazoline N-oxides were synthesized and their antiviral activity against flaviviruses such as TBEV, YFV, and WNV was assessed. Most of the compounds showed activity against TBEV and WNV, and a few also demonstrated inhibitory activity against YFV. The mechanism of action of these compounds appears to affect the early stages of viral replication cycle after cell entry.
ARCHIV DER PHARMAZIE
(2023)
Article
Pharmacology & Pharmacy
Ludek Eyer, Katherine Seley-Radtke, Daniel Ruzek
Summary: Tick-borne encephalitis (TBE) is a potentially fatal disease common in much of Europe and Asia. Currently, there is no specific therapy for TBE patients, but there are ongoing efforts to develop small molecules and monoclonal antibodies that can interfere with the TBE virus life cycle and potentially be used for post-exposure prophylaxis or early therapy. This review summarizes the latest directions and experimental approaches for combating TBE in humans.
ANTIVIRAL RESEARCH
(2023)
Article
Immunology
Ewa Bojkiewicz, Kacper Toczylowski, Sambor Grygorczuk, Beata Zelazowska-Rutkowska, Justyna Dunaj, Agnieszka Zebrowska, Piotr Czupryna, Anna Moniuszko-Malinowska, Artur Sulik
Summary: In Poland, the vaccination rate for tick-borne encephalitis is low, despite a high incidence of severe infections. A study found that many people have asymptomatic infections and have different attitudes towards the vaccine.
Review
Virology
E. Taylor Stone, Amelia K. Pinto
Summary: The Flaviviridae family consists of diverse arthropod-borne viruses that cause globally relevant diseases in humans. Infection with certain flaviviruses, like West Nile virus, Zika virus, Japanese encephalitis virus, tick-borne encephalitis virus, and Powassan virus, can lead to neuroinvasive diseases such as meningitis or encephalitis. T cells have been identified as a contributor to neuroinflammation, but their role in tick-borne flavivirus encephalitis requires further study. Understanding the involvement of T cells in tick-borne flavivirus encephalitis is crucial for improving vaccine safety and efficacy, as well as developing treatments for human disease.
Article
Virology
Xiaohui Li, Hongwei Ji, Di Wang, Lihe Che, Li Zhang, Liang Li, Qing Yin, Quan Liu, Feng Wei, Zedong Wang
Summary: This study identified the presence of Far Eastern subtype of TBEV in Ixodes persulcatus ticks in northeastern China, with genetic variations observed among viruses from different natural foci in the region.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Letter
Immunology
Jana Kerlik, Maria Avdicova, Monika Musilova, Jana Beresova, Roman Mezencev
Summary: This is a case of probable transmission of Tick-borne encephalitis virus (TBEV) from an unvaccinated mother to an infant through breast-feeding. It highlights the importance of being cautious when exposed to infected animals or consuming raw food products, especially for individuals who are not vaccinated.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Immunology
Julia Finkensieper, Leila Issmail, Jasmin Fertey, Alexandra Rockstroh, Simone Schopf, Bastian Standfest, Martin Thoma, Thomas Grunwald, Sebastian Ulbert
Summary: Tick-borne encephalitis virus (TBEV) is a zoonotic flavivirus that can cause severe neurological complications in humans. Traditional methods of producing TBEV vaccines using formaldehyde have drawbacks, and this study suggests that low-energy electron irradiation (LEEI) could be an alternative method. Immunizing mice with LEEI-inactivated TBEV resulted in higher levels of antibodies and complete protection against viral challenge.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Laura Wiesner, Carla Schmutte, Imke Steffen
Summary: Tick-borne encephalitis virus (TBEV) is a serious pathogen that requires heightened biosafety measures in the laboratory. Different inactivation procedures, such as heat, acidic pH, alcohol, formaldehyde, detergents, and UV irradiation, show varying effectiveness against TBEV, depending on sample size and composition.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Microbiology
Mulugeta A. Wondim, Piotr Czupryna, Slawomir Pancewicz, Ewelina Kruszewska, Monika Groth, Anna Moniuszko-Malinowska
Summary: Tick-borne encephalitis is a neuroinfection widely distributed in the Euro-Asia region, primarily transmitted by infected ticks. The total number of confirmed cases reported in Europe from 2000 to 2019 was 51,519, with a decrease in cases in 2014 and 2015 but an increasing trend since 2015.
Article
Microbiology
Nicole Barp, Cinzia Cappi, Marianna Meschiari, Marzia Battistel, Maria Vittoria Libbra, Maria Alice Ferri, Stefano Ballestri, Altea Gallerani, Filippo Ferrari, Marisa Meacci, Mario Sarti, Mariano Capitelli, Cristina Mussini, Erica Franceschini
Summary: The first human case of tick-borne encephalitis virus (TBEV) infection in central Italy has been reported, indicating endemic transmission and a possible hot spot in the Serramazzoni region of the Emilian Apennines.
Article
Chemistry, Physical
Emmanuelle Bignon, Elise Dumont, Antonio Monari
Summary: In this study, we investigated the dynamic behavior of the precursor of tick-borne encephalitis virus membrane fusion protein E at neutral and acidic pH. We found different stabilizations of the E dimer and identified the role of the small fusion-assisting protomer (pr). Understanding the precise regulation of viral maturation at the atomic level is crucial for developing effective strategies against emerging viral threats.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Microbiology
Yuan Bai, Yanfang Zhang, Zhengyuan Su, Shuang Tang, Jun Wang, Qiaoli Wu, Juan Yang, Abulimiti Moming, Yujiang Zhang, Lesley Bell-Sakyi, Surong Sun, Shu Shen, Fei Deng
Summary: This study identified and isolated Karshi virus (KSIV) from ticks in Xinjiang, China. KSIV, closely related to tick-borne encephalitis virus (TBEV), was found to infect humans, mammals, and ticks, and cause encephalitis in suckling mice. The study also detected high prevalence of KSIV among ticks and infections in sheep and marmots. Cross-reactivity between KSIV and TBEV was observed in animal serum samples. These findings suggest a need to distinguish KSIV from TBEV and investigate the prevalence and incidence of both viruses in Northwestern China.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Medicine, General & Internal
Dominic Adam Worku
Summary: Tick-borne encephalitis (TBE) is a viral disease prevalent in large parts of Europe and Asia, characterized by neurological symptoms that can range from mild to severe, and it often leads to long-term morbidity. The pathogenesis of TBE is not fully understood, but factors such as ticks, viral strains, host immune response, and age may contribute to the disease phenotype. The incidence of TBE is increasing and its epidemiology is changing due to global warming.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Parasitology
Emily L. Pascoe, Ankje de Vries, Helen J. Esser, Constantianus J. M. Koenraadt, Hein Sprong
Summary: In this study, it was demonstrated that tick-borne encephalitis virus (TBEV) RNA can be detected in non-destructive samples obtained from naturally infected wild rodents, providing a practical alternative for long-term surveillance of the virus.
PARASITES & VECTORS
(2023)
Article
Immunology
Renata Varnaite, Sara Gredmark-Russ, Jonas Klingstrom
Summary: We assessed the standardized mortality ratio of tick-borne encephalitis (TBE) in Sweden from 2004 to 2017. The standardized mortality ratio for TBE was 3.96 (95% CI 2.55-5.90), and no fatalities were observed in patients under 40 years of age. These findings highlight the importance of further vaccination efforts in populations at risk for TBE.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Anatomy & Morphology
Dmitry Bagrov, Grigory S. Glukhov, Andrey Moiseenko, Maria G. Karlova, Daniil S. Litvinov, Petr A. Zaitsev, Liubov Kozlovskaya, Anna A. Shishova, Anastasia A. Kovpak, Yury Y. Ivin, Anastasia N. Piniaeva, Alexey S. Oksanich, Viktor P. Volok, Dmitry Osolodkin, Aydar A. Ishmukhametov, Alexey M. Egorov, Konstantin Shaitan, Mikhail P. Kirpichnikov, Olga S. Sokolova
Summary: This study characterized the beta-propiolactone inactivated SARS-CoV-2 virions using TEM and AFM, finding that samples prepared using SEC and IEC retained more spikes on the surface and the S proteins were in the pre-fusion conformation. Analytical TEM showed that the inactivated virions retained nucleic acid, demonstrating their potential as a vaccine candidate.
MICROSCOPY RESEARCH AND TECHNIQUE
(2022)
Article
Chemistry, Medicinal
Kirill S. Sadovnikov, Dmitry A. Vasilenko, Yulia A. Gracheva, Nikolay A. Zefirov, Eugene Radchenko, Vladimir A. Palyulin, Yuri K. Grishin, Vladislav A. Vasilichin, Alexander A. Shtil, Pavel N. Shevtsov, Elena F. Shevtsova, Tamara S. Kuznetsova, Sergei A. Kuznetsov, Alexander S. Bunev, Olga N. Zefirova, Elena R. Milaeva, Elena B. Averina
Summary: A series of novel antimitotic agents were designed by replacing the heterocyclic cores in two tubulin-targeting lead molecules. Compound 1aa showed partial depolymerization of microtubules at a high concentration and complete inhibition of cell growth and viability at nanomolar to submicromolar concentrations. The study also revealed the key structural parameter determining the efficiency towards tubulin and other molecular targets.
ARCHIV DER PHARMAZIE
(2022)
Article
Microbiology
Olga E. Ivanova, Armen K. Shakaryan, Nadezhda S. Morozova, Yulia A. Vakulenko, Tatyana P. Eremeeva, Liubov I. Kozlovskaya, Olga Y. Baykova, Elena Y. Shustova, Yulia M. Mikhailova, Natalia I. Romanenkova, Nadezhda R. Rozaeva, Natela I. Dzhaparidze, Nadezhda A. Novikova, Vladimir V. Zverev, Lyudmila N. Golitsyna, Alexander N. Lukashev
Summary: Surveillance for acute flaccid paralysis syndrome (AFP) in children under 15 is crucial for the Global Polio Eradication Initiative. The identification of non-polio enteroviruses (NPEV) alongside polioviruses in stool samples of AFP cases has provided valuable insights. Enteroviruses A71 and D68 have been definitively linked to AFP, while the association of Enterovirus Coxsackie A2 (CVA2) with AFP is less understood. This study suggests that CVA2 may be a cause of AFP, highlighting the importance of AFP surveillance for both polio control and the study of uncommon AFP agents.
Article
Infectious Diseases
Alexander S. Yakovlev, Ilmira K. Belyaletdinova, Lyudmila N. Mazankova, Elmira R. Samitova, Ismail M. Osmanov, Nataly Gavelya, Viktor P. Volok, Ekaterina S. Kolpakova, Anna A. Shishova, Natalia A. Dracheva, Liubov Kozlovskaya, Galina G. Karganova, Aydar A. Ishmukhametov
Summary: The COVID-19 pandemic had a significant impact on the circulation of non-SARS-CoV-2 respiratory viruses and the clinical characteristics of COVID-19 in hospitalized children. The detection rate of non-SARS-CoV-2 viruses decreased in 2020 compared to 2018, and the median age of children with respiratory viruses increased during the pandemic. There was no significant difference in the frequency of intensive care unit (ICU) admission between children with SARS-CoV-2 and other respiratory virus infections, and the co-detection rates of SARS-CoV-2 with other respiratory viruses were lower than expected.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Organic
D. S. Kuliukhina, A. D. Averin, S. P. Panchenko, A. S. Abel, E. N. Savelyev, B. S. Orlinson, I. A. Novakov, C. R. D. Correia, I. P. Beletskaya
Summary: The N-heteroarylation reactions of n-octylamine and adamantane-containing amines with 2-iodopyridine and 2-bromopyridine and its fluorinated derivatives were studied under the catalysis of CuI and copper nanoparticles. The most efficient ligand in the CuI-catalyzed reactions was found to be 2-isobutyrylcyclohexanone, while 2-isobutyrylcyclohexanone or l-proline exhibited the highest activity in the reactions catalyzed by copper nanoparticles. The yields of the reactions catalyzed by copper nanoparticles were equal to or higher than those in the CuI-catalyzed processes. Additionally, the possibility of nanocatalyst recycling was demonstrated, showing no loss of activity after 6 cycles.
RUSSIAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Immunology
Nadezhda V. Yagovkina, Lev M. Zheleznov, Ksenia A. Subbotina, Andrey A. Tsaan, Liubov I. Kozlovskaya, Ilya V. Gordeychuk, Anastasia K. Korduban, Yury Y. Ivin, Anastasia A. Kovpak, Anastasia N. Piniaeva, Anna A. Shishova, Elena Y. Shustova, Yusuf K. Khapchaev, Galina G. Karganova, Alexandra A. Siniugina, Tatiana V. Pomaskina, Aleksandr A. Erovichenkov, Konstantin Chumakov, Aydar A. Ishmukhametov
Summary: A study conducted at Kirov Medical University found that immunization with bivalent Oral Poliovirus Vaccine (bOPV) can reduce the incidence of COVID-19, confirming the non-specific protective effect of live attenuated vaccines against off-target infections.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Viktoriya G. Krut', Sergei A. Chuvpilo, Irina V. Astrakhantseva, Liubov I. Kozlovskaya, Grigory A. Efimov, Andrei A. Kruglov, Marina S. Drutskaya, Sergei A. Nedospasov
Summary: This review discusses the potential of using synthetic and recombinant peptides and polypeptides for the prevention of COVID-19 by blocking the interaction between the virus and its main receptor ACE2, as well as components of antiviral vaccines, particularly against new emerging virus variants.
BIOCHEMISTRY-MOSCOW
(2022)
Article
Pharmacology & Pharmacy
Alexey A. Chistov, Stepan P. Chumakov, Igor E. Mikhnovets, Timofei D. Nikitin, Nikita A. Slesarchuk, Victoria I. Uvarova, Anna A. Rubekina, Yulia V. Nikolaeva, Eugene V. Radchenko, Evgeny V. Khvatov, Alexey A. Orlov, Vasilisa S. Frolenko, Maksim Sukhorukov, Ekaterina S. Kolpakova, Elena Y. Shustova, Anastasiya Galochkina, Philipp P. Streshnev, Eugene M. Osipov, Ksenia A. Sapozhnikova, Andrey V. Moiseenko, Vladimir A. Brylev, Gleb V. Proskurin, Yuri S. Dokukin, Sergey V. Kutyakov, Andrey V. Aralov, Vladimir A. Korshun, Sergei V. Strelkov, Vladimir A. Palyulin, Aydar A. Ishmukhametov, Evgeny A. Shirshin, Dmitry I. Osolodkin, Anna A. Shtro, Liubov I. Kozlovskaya, Vera A. Alferova, Alexey V. Ustinov
Summary: A new series of compounds based on a 5-(perylen-3-ylethynyl)-uracil scaffold showed potent broad-spectrum antiviral activity against arthropod-borne viruses (TBEV, YFV, and CHIKV) as well as respiratory viruses (SARS-CoV-2 and IAV). The compounds inhibited viral fusion and caused damage to the viral envelope, suggesting a photoinduced ROS-mediated mode of antiviral action.
ANTIVIRAL RESEARCH
(2023)
Article
Chemistry, Medicinal
Kseniya N. Sedenkova, Victoria I. Uvarova, Anna A. Nazarova, Aleksandra V. Peisikova, Evgeny V. Khvatov, Maksim V. Sukhorukov, Vasilisa S. Frolenko, Alexander S. Goryashchenko, Ivan S. Kholodilov, Yuri K. Grishin, Viktor B. Rybakov, Marat T. Makenov, Evgeny S. Morozkin, Lyudmila S. Karan, Liubov I. Kozlovskaya, Aydar A. Ishmukhametov, Dmitry I. Osolodkin, Elena B. Averina
Summary: A series of tetrahydroquinazoline N-oxides were synthesized and their antiviral activity against flaviviruses such as TBEV, YFV, and WNV was assessed. Most of the compounds showed activity against TBEV and WNV, and a few also demonstrated inhibitory activity against YFV. The mechanism of action of these compounds appears to affect the early stages of viral replication cycle after cell entry.
ARCHIV DER PHARMAZIE
(2023)
Article
Virology
Liubov I. I. Kozlovskaya, Dmitry I. I. Osolodkin, Ksenia K. K. Tuchynskaya, Anastasia S. S. Shevtsova, Egor V. V. Okhezin, Vladimir A. A. Palyulin, Nikolai A. A. Nikitin, Galina G. G. Karganova
Summary: This study investigates the effects of charge-changing point mutations on the structure and conformational stability of the E protein in TBEV virus. The findings suggest relationships between E protein dynamics and viral neuroinvasiveness, and are supported by experimental evaluations.
ARCHIVES OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vladimir D'yachenko, Dmitry Danilov, Yaroslav Kuznetsov, Semyon Moiseev, Vladimir Mokhov, Vladimir Burmistrov, Gennady Butov
Summary: A one-step method for preparing 1-[isocyanato(phenyl)methyl]adamantane and 1-[isocyanato(phenyl)methyl]-3,5-dimethyladamantane was described, with yields of 95% and 89%, respectively. The method involves the direct inclusion of an adamantane moiety followed by hydrolysis. The resulting ureas showed promising inhibitory activity against human soluble epoxide hydrolase.
Article
Chemistry, Physical
Olga V. Ryzhikova, Kseniya N. Sedenkova, Sergey V. Kositov, Victor A. Tafeenko, Yuri K. Grishin, Elena B. Averina
Summary: In this study, a series of hydroxyalkyltriazoles with a cyclooctane core were synthesized by reacting azidoalcohols or diazidodiols with phenylacetylene. It was found that one of the obtained compounds can act as an effective ligand for CuAAC reactions. X-ray diffraction analysis revealed that this compound forms a complex with copper ions by coordinating only one N-atom of the triazole ring.
News Item
Virology
Thomas S. Postler, Martin Beer, Bradley J. Blitvich, Jens Bukh, Xavier de Lamballerie, J. Felix Drexler, Allison Imrie, Amit Kapoor, Galina G. Karganova, Philippe Lemey, Volker Lohmann, Peter Simmonds, Donald B. Smith, Jack T. Stapleton, Jens H. Kuhn
Summary: This review provides an overview of the recent changes in genus and species nomenclature within the virus family Flaviviridae. The changes were made to address potential ambiguity and to adhere to binomial format conventions. The genus name Flavivirus was changed to Orthoflavivirus, and all species names were modified accordingly. The revised taxonomy of the Flaviviridae family approved by the ICTV in April 2023 is outlined.
ARCHIVES OF VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kseniya N. Sedenkova, Artem S. Sazonov, Dmitry A. Vasilenko, Kristian S. Andriasov, Marina G. Eremenko, Yuri K. Grishin, Evgeny V. Khvatov, Alexander S. Goryashchenko, Victoria I. Uvarova, Dmitry I. Osolodkin, Aydar A. Ishmukhametov, Elena B. Averina
Summary: Spirocyclic compounds containing heterocyclic moieties have shown potential as 3D scaffolds for drug design. In this study, a series of 3-[N,N-bis(sulfonyl)amino]isoxazolines containing spiro-annulated cyclooctane rings were evaluated for their antiviral activity against tick-borne encephalitis (TBEV), yellow fever (YFV), and West Nile (WNV) viruses. The compounds demonstrated antiviral activity against both TBEV and WNV, with the most active derivatives having p-nitrophenyl or lipophilic substituents in the sulfonyl moiety.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Organic
Faiza Diaba, Gisela Trenchs
Summary: The first violet light-mediated synthesis of gamma- and delta-lactams from N-alkenyl trichloroacetamides is reported in this paper. The reactions are conducted in tetrahydrofuran or 2-methyltetrahydrofuran as the sole solvent without catalysts or additives, under non-anhydrous conditions in an air atmosphere where the solvent serves as the radical initiator.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Feroze Hussain, Sajjad Ahmed, Ashiq Hussain Padder, Qazi Naveed Ahmed
Summary: This study reports a novel and efficient one-pot synthesis method for mixed phosphorotrithioates, which does not require supplementary additives and shows broad applicability.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Hyunjin Oh, Ikyon Kim
Summary: A new 1,2,4-triazole-pyrrolo[1,2-a]pyrazine hybrid system, 6-acylpyrrolo[1,2-a][1,2,4]triazolo[5,1-c]pyrazine, was synthesized using a catalyst-free method. This method involved sequential exposure of pyrrole-2-carbonitrile-derived substrates to DMF-DMA and acyl hydrazide, resulting in the formation of acylated pyrazine and 1,2,4-triazole rings, enabling the installation of various substituents at specific positions on the core skeleton.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Ming Yan, Si-fan Wang, Yong-po Zhang, Jin-zhong Zhao, Zhuo Tang, Guang-xun Li
Summary: Here we developed an efficient photocatalytic approach for the convenient preparation of sulfinamides. The reaction allows for the gram-scale preparation of sulfinamides and the one-pot synthesis of various sulfonyl amides.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Farzaneh Bandehali-Naeini, Zahra Tanbakouchian, Noushin Farajinia-Lehi, Nicolas Mayer, Morteza Shiri, Martin Breugst
Summary: Two tandem catalytic systems were developed for the synthesis of novel 3,4-disubstituted maleimides using the same Ugi adducts. Different maleimide structures can be synthesized using either Pd or Cu catalysis.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Article
Chemistry, Organic
Tanya Raghava, Anjan Chattopadhyay, Subhadeep Banerjee, Nivedita Sarkar
Summary: Amine substitution of two ortho fluorine atoms of tetrafluoroterephthalonitrile through SNAr chemistry is easily achievable. But further fluorine substitution is only possible under forcing conditions, yielding valuable fluorophores for bioimaging.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
Review
Chemistry, Organic
Anju Chadha, Santosh Kumar Padhi, Selvaraj Stella, Sowmyalakshmi Venkataraman, Thangavelu Saravanan
Summary: Alcohol dehydrogenases are enzymes that use cofactors for oxidation or reduction reactions of alcohols or carbonyl compounds. They are utilized in green chemistry and have applications in the production of pharmaceuticals. Recombinant enzymes have solved the challenge of producing purified enzymes in large quantities. Engineered alcohol dehydrogenases have been used in asymmetric synthesis in industry. Various methods have been established for regenerating expensive cofactors to make the enzymatic process more efficient and economically viable.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2024)
