Editorial Material
Endocrinology & Metabolism
Zhongjie Fu, Lois E. H. Smith
Summary: Pathologic angiogenesis in eye diseases leading to blindness can be attributed to cell senescence, as demonstrated by the bioinformatics study conducted by Crespo-Garcia, Tsuruda, and Dejda. Their findings were validated in human and mouse retina models, where clearance of senescent cells effectively suppressed neovessel growth.
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Medicine, Research & Experimental
Fatma Sogut, Mahmut Akilli, Ayse Humeyra Kaynar, Handan Tuncel, Deniz Kibar, Sakir Necat Yilmaz, Ulku Comelekoglu
Summary: In this study, the interaction between silica nanoparticles and ion channels in retinal pigment epithelial (RPE) cells was observed using the whole-cell patch clamp technique. The results showed that the windowed scalogram entropy method was sensitive to the effect of silica nanoparticles on RPE cell TRP currents. Additionally, the windowed scale index method could analyze the temporal fluctuations in the aperiodicity of cell membrane current signals. The findings suggest that entropy measurement methods may be useful for the function analysis of cell ion channels.
MEDICAL HYPOTHESES
(2023)
Article
Neurosciences
Erwin Vera, Isabel Cornejo, Maria Isabel Niemeyer, Francisco Sepulveda, L. Pablo Cid
Summary: Kir7.1 K+ channel expressed in retinal pigment epithelium is mutated in inherited retinal degeneration diseases. Mutations changing Arg 162 to Trp or Gln interfere with channel function, while smaller volume amino acids are tolerated or even enhance function. R162, K164, and possibly K159 form crucial binding sites for phosphoinositide, essential for channel activity.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Chemistry, Multidisciplinary
Yu Zhao, Shuliang Gao, Donghui Song, Zhongfeng Ye, Ruijie Xu, Ying Luo, Qiaobing Xu
Summary: Researchers have developed an artificial compartment system based on lipidoids that can control enzyme activity using light. This system utilizes the reversible photoisomerization of azobenzene lipidoids to regulate the permeability of the compartment membrane and control enzyme activity. Unlike traditional inhibitors or noninhibitor systems, this approach does not require mutation or modification of enzymes, resulting in minimal loss of enzyme activity. Furthermore, this method provides a generic strategy for controlling multiple enzymes and has the potential to be applied in biological mechanism research and precision medicine.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Ophthalmology
M. Helena Cardoso, Michael J. Hall, Thomas Burgoyne, Pedro Fale, Tina Storm, Cristina Escrevente, Pedro Antas, Miguel C. Seabra, Clare E. Futter
Summary: This study aimed to model the effects of chloroquine on the retinal pigment epithelium in vivo and investigate the impact of mild chloroquine treatment on lysosome function and turnover. The research found that low-dose chloroquine treatment inhibits lysosome reformation, leading to a gradual depletion of lysosomes' ability to interact with cargo-carrying vacuoles and degrade their content. The accumulation of pro-cathepsin D and LAMP1 reflects the inhibition of normal turnover of lysosomal constituents and possibly lysosomes themselves. Understanding the mechanisms underlying lysosome reformation may contribute to the development of new treatments for chloroquine-induced retinopathy.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2023)
Article
Multidisciplinary Sciences
Lyndsay L. Leach, Nicholas J. Hanovice, Stephanie M. George, Ana E. Gabriel, Jeffrey M. Gross
Summary: Research has revealed that zebrafish RPE has strong intrinsic regenerative potential, with elements of immune response identified as critical mediators in the regeneration process. Macrophage/microglia cells are responsive to RPE damage and essential for the timely progression of regenerative response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Bao-ming Wu, Jaree Bargaineer, Ling Zhang, Tao Yang, Zhi-gang Xiong, Tian-dong Leng
Summary: Oxidative stress is closely related to neurodegenerative diseases and acute injuries. The study found that hydrogen peroxide can increase ASIC1a expression and activation through the JNK signaling pathway, shedding light on the pathogenesis of neurological disorders involving ROS and ASIC1a activation.
ACTA PHARMACOLOGICA SINICA
(2021)
Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Ophthalmology
Romain Touze, Marc M. Abitbol, Dominique Bremond-Gignac, Matthieu P. Robert
Summary: This study confirms the dysfunction of the retinal pigment epithelium (RPE) in patients with neurofibromatosis type 1 (NF1), characterized by a lower dark trough (DT) and a corresponding higher light peak (LP)/DT ratio. This pattern may be due to a dysregulation of melanocytogenesis, leading to disrupted calcium ion flux and abnormal polarization of the RPE.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Review
Pharmacology & Pharmacy
Song Yang, Jun Zhou, Dengwen Li
Summary: The retinal pigment epithelium is crucial for visual functions, but damage to its structure and function can lead to various retinopathies with no cure currently available. Understanding the development, function, and pathobiology of the retinal pigment epithelium is essential for preventing and treating retinopathies. This review discusses potential strategies for treatment and provides new perspectives for future research in this field.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Rocio K. Finol-Urdaneta, Jeffrey R. McArthur, Ashraf Aboelela, Richard S. Bujaroski, Hiwa Majed, Alejandra Rangel, David J. Adams, Marie Ranson, Michael J. Kelso, Benjamin J. Buckley
Summary: Acid-sensing ion channels (ASICs) are transmembrane sensors of extracellular acidosis and potential drug targets. In this study, 6-iodoamiloride was identified as a potent inhibitor of ASIC1 and ASIC3, showing improved inhibitory activity compared to amiloride. This compound can be used to study the effects of ASIC inhibition on cell physiology.
MOLECULAR PHARMACEUTICS
(2023)
Article
Neurosciences
Miguel Salinas, Pascal Kessler, Dominique Douguet, Daad Sarraf, Nicolo Tonali, Robert Thai, Denis Servent, Eric Lingueglia
Summary: This study elucidated the inhibition mechanism of the snake venom toxin Mamb-1 on the acid-sensing ion channel ASIC1a through structure-activity relationship, molecular dynamics simulation, and experimental research. It provides important insights for further improvement and optimization of ASIC blockers.
Article
Chemistry, Analytical
Fanny Girard-Sahun, Pauline Lefrancois, Vasilica Badets, Stephane Arbault, Franck Clement
Summary: This study aims to detect the in situ production of reactive oxygen and nitrogen species (RONS), specifically superoxide anion (O-2(center dot-)), in aqueous buffer solutions exposed to cold atmospheric plasmas (CAPs). The production and accumulation of RONS in the liquids were found to depend on the plasma composition, with N-2-based shieldings providing the highest concentrations of superoxide. The study provides valuable insights into the interactions between CAPs and physiological solutions for biomedical applications.
ANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Biola F. Egbowon, Enzo Fornari, Joseph M. Pally, Alan J. Hargreaves, Bob Stevens, T. Martin Mcginnity, Barbara K. Pierscionek
Summary: This study demonstrates that human RPE cell lines (ARPE-19) can be cultured on ultrathin suspended electrospun nanofibre scaffolds (ENS) composed of hydrophobic polymer polyacrylonitrile (PAN) and a water-soluble aliphatic diamine, without or with fluocinolone acetonide (FA) treatment. The cells survived and maintained their morphology for up to 150 days with FA-treated ENS, showing an epithelial phenotype with biomarkers critical for retinal characteristics. This technique has potential for subretinal transplantation.
MATERIALS & DESIGN
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Diane S. Abou, Amanda Fears, Lucy Summer, Mark Longtine, Nadia Benabdallah, Ryan C. Riddle, David Ulmert, Jeff M. Michalski, Richard L. Wahl, Denise Chesner, Michele Doucet, Nicholas C. Zachos, Brian W. Simons, Daniel L. J. Thorek
Summary: The study investigates the role of enteric ion channels in improving the uptake of Ra-223 and reducing toxicity. In vivo experiments showed that amiloride can enhance bone uptake and inhibit tumor growth when combined with other agents. This combination therapy has the potential to improve outcomes for bone-metastatic cancer patients while maintaining tolerability.
JOURNAL OF NUCLEAR MEDICINE
(2021)