Article
Biochemistry & Molecular Biology
Ning-Ping Foo, Yu-Fan Liu, Ping-Ching Wu, Chung-Hsi Hsing, Bu-Miin Huang, Edmund-Cheung So
Summary: This study found that MDZ suppressed the current density of I-K(DR) in a concentration-, time-, and state-dependent manner in Jurkat T-lymphocytes, and affected immune-regulating cytokine expression in LPS/PMA-treated human T lymphocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Valentina Back, Amir Asgari, Aleksandra Franczak, Max Saito, Diego Castaneda Zaragoza, Shaun L. Sandow, Frances Plane, Paul Jurasz
Summary: This study investigated the role of platelet IKCa channels in platelet function and NO generation. The results showed that activation of IKCa channels can inhibit platelet aggregation by reducing calcium signaling and granule secretion. This finding provides a new target for the development of antiplatelet drugs that limit atherothrombosis.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Pharmacology & Pharmacy
Shuai Guo, Zhenhui Chen, Peng-Sheng Chen, Michael Rubart
Summary: Ondansetron inhibits hSK2 currents even under altered ionic conditions, potentially showing antiarrhythmic effects.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Taro Koya, Masaya Watanabe, Hiroyuki Natsui, Takahide Kadosaka, Takuya Koizumi, Motoki Nakao, Hikaru Hagiwara, Rui Kamada, Taro Temma, Toshihisa Anzai
Summary: This study found that inhibiting nNOS can increase the inducibility of atrial arrhythmia, and a specific SK channel blocker called apamin can reverse the increased inducibility of arrhythmia. Apamin can mitigate the abbreviation of APD without altering Ca2+ transient, suggesting an underlying mechanism of posttranslational modification of SK channels.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Te-Yu Hung, Huai-Ying Ingrid Huang, Sheng-Nan Wu, Chin-Wei Huang
Summary: The study revealed that the inhibitory effect of Vigabatrin on IKCa channels in glioblastoma multiforme cells may be an important mechanism underlying its anti-tumor actions.
BMC PHARMACOLOGY & TOXICOLOGY
(2021)
Article
Physiology
Beth A. McNally, Amber E. Plante, Andrea L. Meredith
Summary: Daily regulation of BK channel activity in the suprachiasmatic nucleus (SCN) is dependent on the type of Ca2+ channels, with LTCCs playing a major role during the day and less so at night. SCN neurons lack a specific subtype of Ca2+ channels responsible for BK current activation, suggesting a complex interplay between different LTCC subtypes.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Cell Biology
Xiaoling Jia, Qingmao Yang, Chao Gao, Xinlan Chen, Yanan Li, Hao Su, Yufan Zheng, Shuwen Zhang, Ziyu Wang, Haikun Wang, Lin-Hua Jiang, Yan Sun, Yubo Fan
Summary: This study demonstrates that on substrates with pathologically relevant stiffness, the upregulation of the IKCa channel enhances intracellular Ca2+ signaling and activates the ERK signal pathway to promote VSMC proliferation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Hiroaki Kito, Susumu Ohya
Summary: Osteoblasts are crucial in bone modeling and remodeling, with their differentiation being regulated by various signaling pathways. Proper bone homeostasis depends on the differentiation and function of osteoblast lineage cells. Ca2+ signaling controls many processes in osteoblast lineage cells, and K+ channel activation indirectly influences Ca2+ signaling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Ning-Ning Ji, Lei Du, Ying Wang, Ke Wu, Zi-Yang Chen, Rong Hua, Yong-Mei Zhang
Summary: Visceral hypersensitivity in irritable bowel syndrome is associated with the downregulation of membrane SK2 in the hypothalamic paraventricular nucleus. Activation of SK2 may alleviate visceral hypersensitivity, suggesting SK2 activators as a potential intervention for visceral pain.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Physiology
Concetta Caglioti, Federico Palazzetti, Lorenzo Monarca, Raffaele Lobello, Maria Rachele Ceccarini, Rossana Giulietta Iannitti, Roberta Russo, Francesco Ragonese, Chiara Pennetta, Antonella De Luca, Michela Codini, Bernard Fioretti
Summary: Glioblastomas (GBs) are highly malignant and invasive tumors in the central nervous system. Phosphatidylinositol 3-kinase (PI3K) activity and intermediate conductance calcium-activated potassium (KCa3.1) current play important roles in GB biology. This study found that KCa3.1 channel activity can be modulated by PI3KC2 beta, leading to a reduction in intracellular calcium concentration and open probability. The findings have therapeutic implications for preventing chemo- and radioresistance mechanisms in GB.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ming-Chi Lai, Sheng-Nan Wu, Chin-Wei Huang
Summary: The antiepileptic drug Rufinamide was found to affect ionic currents in brain pituitary cells, increasing the amplitude of calcium-activated potassium currents, enhancing the activity of large-conductance calcium-activated potassium channels, and influencing the hysteresis of the currents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Giedrius Kanaporis, Lothar A. Blatter
Summary: This study investigates the effects of SK channel activation on alternans in cardiac myocytes. The results demonstrate that SK channel activation can prevent or reduce pacing-induced alternations in CaT and APD, presenting a potential intervention for arrhythmia prevention and therapy.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Maurish Bukhari, Han Deng, Darren Sipes, Marisa Ruane-Foster, Kayla Purdy, Craig D. Woodworth, Shantanu Sur, Damien S. K. Samways
Summary: This study reveals that elevated cytosolic Ca2+ levels stimulate rapid uptake and nuclear accumulation of a DNA-binding fluorescent dye in cervical cancer cells, dependent on activation of intermediate conductance Ca2+-activated K+ channels. Comparisons between cancerous and healthy cervical epithelial cells show significant differences in functional K+ channel activity, which correlates with the incidence of dye uptake. Activation of these channels sensitizes cancer cells to growth suppressant effects, suggesting potential use of channel activators to enhance penetration of toxins into cancer cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Gergo Bitay, Noemi Toth, Szilvia Deri, Jozefina Szlovak, Zsofia Kohajda, Andras Varro, Norbert Nagy
Summary: Sinus pacemaking relies on the interaction between intracellular Ca2+ handling and surface membrane ion channels, with the small-conductance Ca2+-activated K+-channel (I-SK) potentially playing a significant role. This study investigated the role of I-SK in rabbit SAN cells and found that under normal conditions, I-SK has no impact on pacemaking, but it moderately contributes to SAN automaticity under beta-adrenergic activation.
Article
Physiology
Nicolas Montalbetti, Aneta J. Przepiorski, Shujie Shi, Shaohu Sheng, Catherine J. Baty, Joseph C. Maggiore, Marcelo D. Carattino, Thitinee Vanichapol, Alan J. Davidson, Neil A. Hukriede, Thomas R. Kleyman
Summary: This study examines the functional expression of key ion channels in kidney organoids derived from pluripotent stem cells. It demonstrates the presence of large-conductance Ca2+-activated K+ (BKCa) channels, renal outer medullary K+ (ROMK, Kir1.1) channels, and epithelial Na+ channels (ENaC) in different cell populations within the organoids. These findings suggest that human kidney organoids can functionally produce important distal nephron K+ and Na+ channels.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)