Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 61, Issue 4, Pages 313-324Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155413477115
Keywords
pronephros; podocytes; slit diaphragm; nephrin; glomerulogenesis; zebrafish; medaka
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Funding
- University of Oklahoma Health Sciences Center (OUHSC)
- Ministry of Education, Culture, Sports, Science and Technology of Japan [23590226]
- NIH [R21-DK069604, R01-DK078209]
- Grants-in-Aid for Scientific Research [23590226] Funding Source: KAKEN
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Slit diaphragm (SD) is a highly specialized intercellular junction between podocyte foot processes and plays a crucial role in the formation of the filtration barrier. In this study, we examined the developmental localization of Nephrin, an essential component of SD, in the pronephric glomerulus of zebrafish and medaka. In the mature glomerulus of both fish, Nephrin is localized along the glomerular basement membrane as seen in mammals, indicating that Nephrin is localized at the SD. Interestingly, Nephrin was detected already in immature podocytes before the SD and foot processes started to form in both fish. Nephrin was localized along the cell surface of immature podocytes but as different localization patterns. In zebrafish, Nephrin signal bordered the lateral membrane of podocytes, which were columnar in shape, as in rat immature podocytes. However, in medaka immature podocytes, Nephrin was localized in a punctate pattern among podocyte cell bodies. These findings suggest that Nephrin needs to be integrated to the membrane before the formation of the SD and then moves to the proper site to form the SD. Furthermore, a podocyte-specific marker, such as Nephrin, should be a useful tool for the future analysis of pronephric glomerular development in fish mutants and morphants.
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