Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 58, Issue 11, Pages 1033-1043Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/jhc.2010.956771
Keywords
femoral head; cartilage; dentin matrix protein 1; dentin sialophosphoprotein; bone sialoprotein; osteopontin; chondrogenesis; proteoglycan
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Funding
- National Institutes of Health [DE-005092]
- Department of Science and Technology of Heilongjiang Province of China [GC09C412-1]
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The small integrin-binding ligand, N-linked glycoprotein (SIBLING) family is closely related to osteogenesis. Until recently, little was known about their existence in articular cartilage. In this study, we systematically evaluated the presence and distribution of four SIBLING family members in rat femoral head cartilage: dentin matrix protein 1 (DMP1), bone sialoprotein (BSP), osteopontin (OPN), and dentin sialophosphoprotein (DSPP). First, non-collagenous proteins were extracted and then separated by ion-exchange chromatography. Next, the protein extracts eluted by chromatography were analyzed by Stains-all staining and Western immunoblotting. IHC was used to assess the distribution of these four SIBLING family members in the femoral head cartilage. Both approaches showed that all the four SIBLING family members are expressed in the femoral head cartilage. IHC showed that SIBLING members are distributed in various locations throughout the articular cartilage. The NH2-terminal fragments of DMP1, BSP, and OPN are present in the cells and in the extracellular matrix, whereas the COOH-terminal fragment of DMP1 and the NH2-terminal fragment of DSPP are primarily intracellularly localized in the chondrocytes. The presence of the SIBLING family members in the rat femoral head cartilage suggests that they may play important roles in chondrogenesis. (J Histochem Cytochem 58:1033-1043, 2010)
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