Design, Synthesis and SAR Study of Novel Spiro [Pyrimido[5,4-b]Quinoline-10,5-Pyrrolo[2,3-d]Pyrimidine] Derivatives as Promising Anticancer Agents

Novel spiro [pyrimido[5,4-b]quinoline-10,5-pyrrolo[2,3-d]pyrimidine] derivatives were designed and synthesized, and their chemical structures were confirmed by IR, NMR, elemental analysis, and mass spectral analysis. The anticancer activities of the newly synthesized compounds were evaluated in vitro against four human cancer cell lines including A431, PC-3, MCF-7, and MCF-10A by MTT assay. The screening results showed that three compounds (4m, 4q, and 4s) exhibited potent cytotoxic activities with IC50 values between 7.82 and 9.88M against human breast cancer cell line (MCF-7). Further in vitro studies revealed that inhibition of Sunitinib could be the possible mechanism of action of these molecules.