Journal
OPTICS LETTERS
Volume 40, Issue 11, Pages 2553-2556Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.40.002553
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Funding
- NSF IDBR grant [DBI-1353757]
- NIH [DC00566, DC004657, HD041697]
- Butcher Foundation
- Div Of Industrial Innovation & Partnersh
- Directorate For Engineering [1034720] Funding Source: National Science Foundation
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We report a miniature, lightweight fiber-coupled confocal fluorescence microscope that incorporates an electrowetting variable focus lens to provide axial scanning for full three-dimensional (3D) imaging. Lateral scanning is accomplished by coupling our device to a laser-scanning confocal microscope through a coherent imaging fiber-bundle. The optical components of the device are combined in a custom 3D-printed adapter with an assembled weight of <2 g that can be mounted onto the head of a mouse. Confocal sectioning provides an axial resolution of similar to 12 mu m and an axial scan range of similar to 80 mu m. The lateral field-of-view is 300 mu m, and the lateral resolution is 1.8 mu m. We determined these parameters by imaging fixed sections of mouse neuronal tissue labeled with green fluorescent protein (GFP) and fluorescent bead samples in agarose gel. To demonstrate viability for imaging intact tissue, we resolved multiple optical sections of ex vivo mouse olfactory nerve fibers expressing yellow fluorescent protein (YFP). (C) 2015 Optical Society of America
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