Journal
JOURNAL OF GENETICS AND GENOMICS
Volume 37, Issue 6, Pages 389-U52Publisher
SCIENCE PRESS
DOI: 10.1016/S1673-8527(09)60057-6
Keywords
aquaporin; boron; toxicity tolerance
Funding
- National Basic Research Program of China [2006CB100100]
- National Natural Science Foundation of China [30370129, 30770188]
- 111 Project [B06003]
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Boron (B) toxicity to plants is responsible for low crop productivity in many regions of the world. Here we report a novel and effective means to alleviate the B toxicity to plants under high B circumstance. Functional characterization of AtTIP5;1, an aquaporin gene, revealed that overexpression of AtTIP5;1 (OxAtTIP5;1) in Arabidopsis significantly increased its tolerance to high B toxicity. Compared to wild-type plants, OxAtTIP5;1 plants exhibited longer hypocotyls, accelerated development, increased silique production under high B treatments. GUS staining and quantitative RT-PCR (qRT-PCR) results demonstrated that the expression of AtTIP5; 1 was induced by high B concentration treatment. Subcellular localization analysis revealed that the AtTIP5;1-GFP fusion protein was localized on the tonoplast membrane, which was consistent with the prediction based on bioinformatics. Taken together, our results suggest that AtTIP5;1 is involved in B transport pathway possibly via vacuolar compartmentation for B, and that overexpression of AtTIP5; 1 in plants may provide an effective way to overcome the problem resulting from high B concentration toxicity.
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