Article
Multidisciplinary Sciences
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Summary: Genome remethylation is crucial for mammalian development, with a study showing that deficiencies in Dnmt3a and Dnmt3b lead to ES cells developing into trophoblast cells due to failed suppression of Ascl2. Regulating the expression of Ascl2 can impact the transdifferentiation and contribution of ES cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Yingjie Hang, Xiaoliang Ma, Chunxiao Liu, Siyuan Li, Sixuan Zhang, Ruyan Feng, Qianwen Shang, Qi Liu, Zhaozhao Ding, Xiaoyi Zhang, Liyin Yu, Qiang Lu, Changshun Shao, Hong Chen, Yufang Shi, Jiuyang He, David L. Kaplan
Summary: The development of anisotropic silk protein nanofiber-based hydrogels enables mouse embryonic stem cells to maintain stemness in vitro without the need for leukemia inhibitory factor and mouse embryonic fibroblasts, resulting in superior pluripotency and genetic stability. The hydrogels establish an improved dynamic niche that stimulates autocrine factor secretion and maintains pluripotency and propagation of ESCs, offering a simple and cost-effective option for expansion and utility of ESCs in research and therapy, while providing insights into early mammalian embryogenesis.
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Agriculture, Dairy & Animal Science
Alba V. Ledesma, Maci L. Mueller, Alison L. Van Eenennaam
Summary: The progress made in the derivation and culture of pluripotent stem cells from farm animals has opened up the possibility of creating livestock chimeras that can pass on superior genetics and disseminate useful genomic alterations.
Article
Cell Biology
Anderson Tan, Renuka Prasad, Eek-hoon Jho
Summary: TFEB plays a crucial role in mESCs by regulating PTN to maintain pluripotency, with interactions involving Nanog and Sox2. While high levels of TFEB are expected to enhance autophagy-lysosomal activity, undifferentiated mESCs surprisingly display low basal levels.
CELL DEATH & DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Daniel Olivieri, Eleonora Castelli, Yumiko K. Kawamura, Panagiotis Papasaikas, Ilya Lukonin, Melanie Rittirsch, Daniel Hess, Sebastien A. Smallwood, Michael B. Stadler, Antoine H. F. M. Peters, Joerg Betschinger
Summary: Mouse embryonic stem cells are biased towards producing embryonic endoderm rather than extraembryonic endoderm, which is regulated by transcriptional repressors Hdac3 and Dax1. Pluripotency factors Nr5a2 and Esrrb promote cell type conversion, and perturbation of the barrier extends mESC potency. This study shows that transcriptional repressors stabilize pluripotency by biasing the equilibrium between embryonic and extraembryonic lineages in mESCs.
Article
Cell Biology
Shahnaz Babaei-Abraki, Fereshteh Karamali, Mohammad Hossein Nasr-Esfahani
Summary: Human embryonic stem cells (hESCs) have the potential to differentiate into different types of cells, making them a valuable source for therapeutic applications. However, hESCs are prone to cell death after dissociation, which hinders their use. Recent research has found that hESCs can undergo a form of programmed cell death called ferroptosis, which is different from previously observed cell death processes. Ferroptosis is triggered by an increase in intracellular iron, and it is distinct from other forms of cell death in terms of its biochemical, morphological, and genetic characteristics.
CELLULAR SIGNALLING
(2023)
Article
Biochemistry & Molecular Biology
Kazuko Okamoto, Hideaki Fujita, Yasushi Okada, Soya Shinkai, Shuichi Onami, Kuniya Abe, Kenta Fujimoto, Kensuke Sasaki, Go Shioi, Tomonobu M. Watanabe
Summary: Nanog and Oct4 are core transcription factors that regulate pluripotency maintenance in mouse ESCs. The interaction time between Nanog and its target loci increases upon reduced expression or differentiation onset, suggesting a feedback mechanism for pluripotency maintenance. The expression level and interaction frequency of Nanog and Oct4 are correlated with their fluctuation and depend on ESC differentiation status. The DNA viscoelasticity near Oct4 target locus remains flexible during differentiation, supporting its role in chromatin opening or binding to uncondensed regions. These findings propose a new negative feedback mechanism for pluripotency maintenance via the DNA condensation state-dependent interplay of Nanog and Oct4.
Article
Multidisciplinary Sciences
Kang-Xuan Jin, Rujuan Zuo, Konstantinos Anastassiadis, Arne Klungland, Carsten Marr, Adam Filipczyk
Summary: This study investigates the impact of m(6)A deposition on mRNA on the fate of pluripotent stem cells, revealing that it regulates cell fate through modulation of mRNA stability. The results show that m(6)A depletion activates pErk and pAkt signaling pathways, affecting both the maintenance of pluripotency and lineage commitment decisions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Oliver Brookes, Stephen D. Thorpe, Olga Rigby Evans, Michael C. Keeling, David A. Lee
Summary: This study investigated the relationship between biomechanical properties and pluripotency gene expression in murine embryonic stem cells, revealing considerable variation among cells, with high pluripotency gene expression correlating to lower cell stiffness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Seong-Min Kim, Eun-Ji Kwon, Yun-Jeong Kim, Young-Hyun Go, Ji-Young Oh, Seokwoo Park, Jeong Tae Do, Keun-Tae Kim, Hyuk-Jin Cha
Summary: This study discovered the differential roles of Shp2 in naive and primed pluripotency and proposed the usage of iShp2 instead of iMek1 for the efficient maintenance and establishment of naive pluripotency.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Veronika V. Ermakova, Nikita P. Fokin, Nikolay D. Aksenov, Evgeny I. Bakhmet, Ekaterina V. Aleksandrova, Andrey A. Kuzmin, Alexey N. Tomilin
Summary: The transcription factor Oct4 plays a critical role in maintaining pluripotency and can reprogram somatic cells into a pluripotent state. Identifying the genetic elements that regulate the Oct4-encoding gene, Pou5f1, is important for understanding pluripotency regulation. This study shows that translocating a fragment of the Pou5f1 gene impairs the self-renewal and differentiation of mouse embryonic stem cells, indicating the presence of critical regulatory elements outside the previously determined boundaries of Pou5f1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Puja Agrawal, Sridhar Rao
Summary: Cell fate decisions rely on gene expression changes mediated by regulatory elements like enhancers and CTCF binding sites. These elements play crucial roles in early mammalian development and impact cell-type specific gene expression.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrea Lauria, Guohua Meng, Valentina Proserpio, Stefania Rapelli, Mara Maldotti, Isabelle Laurence Polignano, Francesca Anselmi, Danny Incarnato, Anna Krepelova, Daniela Donna, Chiara Levra Levron, Giacomo Donati, Ivan Molineris, Francesco Neri, Salvatore Oliviero
Summary: The establishment of DNA methylation patterns during mouse early development is crucial for cell fate determination. However, the specific molecular targets and mechanisms of de novo methylation machinery during differentiation are not fully understood. In this study, the researchers identified DNMT3B-dependent regulatory elements that play a critical role in cell fate determination. They found that DNMT3B-dependent DNA methylation is essential for proper differentiation and lineage determination, and ectopic expression of DNMT3B can restore normal gene expression and cell fate. This study provides important insights into the role of DNA methylation in early development and cell fate determination.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Jingsheng Li, Chunhong Dai, Wenyan Xie, Heyao Zhang, Xin Huang, Constantinos Chronis, Ying Ye, Wensheng Zhang
Summary: This study developed a one-step strategy to efficiently target and suppress endogenous pluripotent genes in mouse ESCs and replace their expression with AID-fused transgenes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Letter
Biochemistry & Molecular Biology
Xiaofeng Jin, Rui Fu, Wanwan Zhu, Zhengxin Liu, Tiantian Gu, Guanyi Jiao, Hua Yang, Qi Zhou, Zhiqiang Gao, Xiao-Yang Zhao
JOURNAL OF GENETICS AND GENOMICS
(2015)
Article
Cell & Tissue Engineering
Chenhui Ding, Sunxing Huang, Quan Qi, Rui Fu, Wanwan Zhu, Bing Cai, Pingping Hong, Zhengxin Liu, Tiantian Gu, Yanhong Zeng, Jing Wang, Yanwen Xu, Xiaoyang Zhao, Qi Zhou, Canquan Zhou
STEM CELLS AND DEVELOPMENT
(2015)
Review
Chemistry, Multidisciplinary
Bi Wu, Wei Li, Liu Wang, Zhong-hua Liu, Xiao-yang Zhao
ACTA PHARMACOLOGICA SINICA
(2013)
Article
Cell Biology
Nan Cao, Zumei Liu, Zhongyan Chen, Jia Wang, Taotao Chen, Xiaoyang Zhao, Yu Ma, Lianju Qin, Jiuhong Kang, Bin Wei, Liu Wang, Ying Jin, Huang-Tian Yang
Letter
Cell Biology
Haifeng Wan, Zhengquan He, Mingzhu Dong, Tiantian Gu, Guan-Zheng Luo, Fei Teng, Baolong Xia, Wei Li, Chunjing Feng, Xin Li, Tianda Li, Ling Shuai, Rui Fu, Liu Wang, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou
Letter
Cell Biology
Yongchang Chen, Yiqiang Cui, Bin Shen, Yuyu Niu, Xiaoyang Zhao, Lei Wang, Jianying Wang, Wei Li, Qi Zhou, Weizhi Ji, Jiahao Sha, Xingxu Huang
Letter
Cell Biology
Haifeng Wan, Chunjing Feng, Fei Teng, Shihua Yang, Baoyang Hu, Yuyu Niu, Andy Peng Xiang, Weizhen Fang, Weizhi Ji, Wei Li, Xiaoyang Zhao, Qi Zhou
Article
Cell & Tissue Engineering
Wei Li, Xin Li, Tianda Li, Ming-Gui Jiang, Haifeng Wan, Guan-Zheng Luo, Chunjing Feng, Xiaolong Cui, Fei Teng, Yan Yuan, Quan Zhou, Qi Gu, Ling Shuai, Jiahao Sha, Yamei Xiao, Liu Wang, Zhonghua Liu, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou
Article
Biochemistry & Molecular Biology
Ying Zhang, Fei Teng, Guan-Zheng Luo, Meng Wang, Man Tong, Xiaoyang Zhao, Liu Wang, Xiu-Jie Wang, Qi Zhou
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Ming-Gui Jiang, Tianda Li, Chunjing Feng, Rui Fu, Yan Yuan, Quan Zhou, Xin Li, Haifeng Wan, Liu Wang, Wei Li, Yamei Xiao, Xiao-Yang Zhao, Qi Zhou
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Biochemistry & Molecular Biology
Zhenkun Wang, Chao Sheng, Tianda Li, Fei Teng, Lisi Sang, Fenglin Cao, Ziwei Wang, Wanwan Zhu, Wei Li, Xiaoyang Zhao, Zhonghua Liu, Liu Wang, Qi Zhou
JOURNAL OF GENETICS AND GENOMICS
(2012)
Letter
Biochemistry & Molecular Biology
Tianda Li, Xiao-Yang Zhao, Fei Teng, Xin Li, Minggui Jiang, Wei Li, Xuepeng Wang, Jialiang Wang, Lei Liu, Zhonghua Liu, Liu Wang, Qi Zhou
JOURNAL OF GENETICS AND GENOMICS
(2012)
Letter
Biochemistry & Molecular Biology
Xuepeng Wang, Yan Yuan, Quan Zhou, Haifeng Wan, Mei Wang, Qi Zhou, Xiao-Yang Zhao, Jiahao Sha
JOURNAL OF GENETICS AND GENOMICS
(2014)
Article
Multidisciplinary Sciences
Wei Li, Ling Shuai, Haifeng Wan, Mingzhu Dong, Meng Wang, Lisi Sang, Chunjing Feng, Guan-Zheng Luo, Tianda Li, Xin Li, Libin Wang, Qin-Yuan Zheng, Chao Sheng, Hua-Jun Wu, Zhonghua Liu, Lei Liu, Liu Wang, Xiu-Jie Wang, Xiao-Yang Zhao, Qi Zhou
Letter
Cell Biology
Wei Li, Xue-Jiang Guo, Fei Teng, Xiao-Jun Hou, Zhuo Lv, Shu-Ya Zhou, Ye Bi, Hai-Feng Wan, Chun-Jing Feng, Yan Yuan, Xiao-Yang Zhao, Liu Wang, Jia-Hao Sha, Qi Zhou
JOURNAL OF MOLECULAR CELL BIOLOGY
(2013)
