Journal
JOURNAL OF GENETICS AND GENOMICS
Volume 37, Issue 5, Pages 333-340Publisher
SCIENCE PRESS
DOI: 10.1016/S1673-8527(09)60051-5
Keywords
Brassica rapa; Brassica oleracea; proteomics; molecular marker
Funding
- National Natural Science Foundation of China [30671166, 30971812]
- National Key Project 973 [2006CB101603]
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The diploid species Brassica rapa (genome AA) and B. oleracea (genome CC) were compared by full-scale proteome analyses of seedling. A total of 28.2% of the proteins was common to both species, indicating the existence of a basal or ubiquitous proteome. However, a number of discriminating proteins (32.0%) and specific proteins (39.8%) of the Brassica A and C genomes, respectively, were identified, which could represent potentially species-specific functions. Based on these A or C genome-specific proteins, a number of PCR-based markers to distinguish B. rapa and B. oleracea species were also developed.
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