Article
Agriculture, Dairy & Animal Science
Hao Wu, Wendi Zhou, Haijun Liu, Xudai Cui, Wenkui Ma, Haixin Wu, Guangdong Li, Likai Wang, Jinlong Zhang, Xiaosheng Zhang, Pengyun Ji, Zhengxing Lian, Guoshi Liu
Summary: In this study, ASMT ovarian overexpression transgenic goats were produced using SCNT, and the whole-genome methylation of cloned and WT goats was measured using WGBS. Differences in methylation were mainly observed in intergenic and intronic regions, with CG-type methylation sites showing strong correlation. Functional annotation revealed DMGs enriched in various signaling pathways, with 10 growth and development related genes identified. These results indicate that the differential patterns of DNA methylation between cloned and WT goats are likely due to SCNT, shedding light on the mechanisms of somatic cell cloning technology.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Yao Wang, Chuan Tong, Hanren Dai, Zhiqiang Wu, Xiao Han, Yelei Guo, Deyun Chen, Jianshu Wei, Dongdong Ti, Zongzhi Liu, Qian Mei, Xiang Li, Liang Dong, Jing Nie, Yajing Zhang, Weidong Han
Summary: The study demonstrates that pre-treatment of CAR T cells with a DNA methyltransferase inhibitor enhances anti-tumor activities, cytokine production, and proliferation, allowing for effective eradication of tumors at low doses and improved response to tumor rechallenge.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Rana Falahat, Anders Berglund, Ryan M. Putney, Patricio Perez-Villarroel, Shota Aoyama, Shari Pilon-Thomas, Glen N. Barber, James J. Mule
Summary: The lack or loss of tumor antigenicity is a key mechanism of immune evasion and resistance to T cell-based immunotherapies. Epigenetic regulation, specifically DNA methylation, plays a role in suppressing the STING signaling function in melanoma cells, but this suppression can be reversed through pharmacologic DNA methylation inhibition. Restoring STING signaling improves melanoma cell antigenicity and enhances recognition and killing by cytotoxic T cells, highlighting the functional significance of epigenetic processes in tumor-immune evasion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Alexandra Depince, Pierre-Yves Le Bail, Charlene Rouillon, Catherine Labbe
Summary: The study on goldfish clones suggests that authentic clones can be produced using non-enucleated oocytes, and clone survival rate is unrelated to spawn quality. The first and second cell cycles are critical for clones to reach hatching stage, while DNA methylation reprogramming significantly influences clone morphology quality.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Multidisciplinary
Lirui Wang, Chunlei Zhang, Yuping Hong, Xinhong Li, Tangan Li, Ang Gao, Shaojun Pan, Bin Liu, Han Jin, Daxiang Cui
Summary: The study developed a method of combinatorial delivery of epigenetic modulatory drugs through self-assembly, effectively regulating DNA methylation and histone deacetylation, ultimately inhibiting tumor cell proliferation and promoting cell apoptosis.
Article
Andrology
Maryam Zarei, Boshra Shamaghdari, Zeinab Vahabi, Azam Dalman, Poopak Eftekhari Yazdi
Summary: This study examined the effects of the DNA methyltransferase inhibitor RG108 and the histone deacetylase inhibitor SAHA on the reprogramming parameters of cloned mouse embryos. It was found that both RG108 and SAHA separately improved the rate and quality of cloned mouse embryos, but the effects were not observed when used in combination. Furthermore, RG108 corrected the aberrant expression of key developmental genes in cloned embryos.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hiroto S. Fukushima, Hiroyuki Takeda, Ryohei Nakamura
Summary: Epigenetic modifications undergo erasure and reestablishment after fertilization, with some histone modifications escaping reprogramming. In nonmammalian vertebrates, most histone modifications are thought to be intensively erased and reestablished. However, the histone modifications that escape reprogramming in nonmammalian vertebrates and their potential functional roles remain unknown.
Article
Cell Biology
Jin Sun, Weisheng Zheng, Wenqiang Liu, Xiaochen Kou, Yanhong Zhao, Zehang Liang, Lu Wang, Zihao Zhang, Jing Xiao, Rui Gao, Shaorong Gao, Cizhong Jiang
Summary: The study derived TSCs from blastocysts using different methods and analyzed the dynamics of transcription and DNA methylation, revealing the crucial role of FGF4 treatment in TSC formation. Specific DNA methylation remodeling processes and transcription factor networks play a key role in TSC pluripotency.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Pedro Mikael da Silva Costa, Sarah Leyenne Alves Sales, Daniel Pascoalino Pinheiro, Larissa Queiroz Pontes, Sarah Sant'Anna Maranhao, Claudia do O. Pessoa, Gilvan Pessoa Furtado, Cristiana Libardi Miranda Furtado
Summary: Disruption of the epigenetic program of gene expression is a hallmark of cancer, which is related to tumor heterogeneity, self-renewal, and differentiation. The reversible nature of epigenetic modifications provides a promising approach for cancer treatment by restoring the cancer epigenome through the inhibition of epigenetic modifiers. These alterations can also serve as biomarkers for early cancer diagnosis, and there are already approved epigenetic therapies for cancer treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Tian-En Si, Zhixiao Li, Jingjing Zhang, Songxue Su, Yupeng Liu, Shiyue Chen, Guang-Hua Peng, Jing Cao, Weidong Zang
Summary: Retinal degenerative diseases, which cause neuronal death and vision loss, can be treated by reprogramming Muller glia into stem or progenitor cells to regenerate the retina. Epigenetic mechanisms, such as DNA methylation and histone modification, play a crucial role in the reprogramming process. Understanding these mechanisms is important for developing Muller glial reprogramming therapy for retinal degenerative diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sebastian Canovas, Elena Ivanova, Meriem Hamdi, Fernando Perez-Sanz, Dimitrios Rizos, Gavin Kelsey, Pilar Coy
Summary: The study found that in vitro culture affects DNA methylation, and the sex-specific methylation differences in blastocysts vary in embryos from different sources. Methylation differences were more frequent in the first intron than in CpGi in promoters, and sex produced a stronger bias in the results than embryo origin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Neurosciences
Ricardo Martins-Ferreira, Barbara Leal, Paulo Pinho Costa, Esteban Ballestar
Summary: Microglia play a crucial role in the immune defense of the central nervous system, with high phenotypic plasticity and involvement in neurological pathologies. The concept of innate immune memory and epigenetic reprogramming are closely linked to microglial activation and response in CNS diseases.
PROGRESS IN NEUROBIOLOGY
(2021)
Review
Microbiology
Qun Gao, Shuwei Lu, Yuwei Wang, Longgui He, Mingshu Wang, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Sai Mao, Xumin Ou, Di Sun, Bin Tian, Anchun Cheng
Summary: Epigenetics regulates expression levels of important genes in both prokaryotes and eukaryotes without changing genetic DNA sequences. DNA methylation is a universal epigenetic mechanism in all organisms. In bacteria, DNA methylation is the main form of epigenetic regulation and plays important roles in clinically relevant phenotypes. This review focuses on recent developments in understanding bacterial DNA methyltransferases and their involvement in gene expression regulation, DNA replication, and repair control. The advancements in sequencing techniques have allowed for the characterization of genome-wide epigenetic regulation, which may lead to the development of novel epigenetic inhibitors for biomedical applications.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Marina Barcena-Varela, Hannah Paish, Laura Alvarez, Iker Uriarte, Maria U. Latasa, Eva Santamaria, Miriam Recalde, Maria Garate, Alex Claveria, Leticia Colyn, Maria Arechederra, Maria J. Iraburu, Malgorzata Milkiewicz, Piotr Milkiewicz, Bruno Sangro, Stuart M. Robinson, Jeremy French, Ana Pardo-Saganta, Julen Oyarzabal, Felipe Prosper, Krista Rombouts, Fiona Oakley, Jelena Mann, Carmen Berasain, Matias A. Avila, Maite G. Fernandez-Barrena
Summary: The study examined the efficacy of a novel dual reversible G9a/DNMT1 inhibitor CM272 in halting liver fibrogenesis, finding that it inhibited fibrogenic responses and restored normal gene expression and mitochondrial function.
Article
Biochemistry & Molecular Biology
Aiwen Jiang, Yangyang Ma, Xue Zhang, Qianqian Pan, Pengfei Luo, Hongyun Guo, Wangjun Wu, Juan Li, Tong Yu, Honglin Liu
Summary: This study investigates the aberrant epigenetic reprogramming during the formation of piPSCs in pigs. It reveals that incomplete chromatin remodeling and DNA demethylation hinder pluripotent activation, resulting in low expression of endogenous pluripotency genes. The study also highlights the abnormal expression of potential imprinted genes in piPSCs, providing insight into porcine-iPSC reprogramming and genome imprinting.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)