Article
Virology
Maite Arrizubieta, Oihane Simon, Adriana Ricarte-Bermejo, Miguel Lopez-Ferber, Trevor Williams, Primitivo Caballero
Summary: This study examines the consequences of coocclusion of two different viruses, HearSNPV and HearMNPV, on the pathogenicity, stability, and host range of mixed-virus occlusion bodies (OBs). The results show that HearSNPV is more pathogenic, has higher replication efficiency, and can infect a broader range of hosts compared to HearMNPV. However, the cooccluded mixtures are unstable in serial passage, with one virus dominating depending on the host species.
Article
Virology
Lulu Zhao, Xingjian Liu, Kai Tang, Zhifang Zhang, Huan Zhang, Yinu Li
Summary: This study characterized the biological and genetic features of HearNPV-BJ, a strain of Helicoverpa assulta nucleopolyhedrovirus. The whole genome sequence analysis revealed its high similarity with other Helicoverpa armigera nucleopolyhedroviruses and suggested that HearNPV-BJ may be a variant of this species.
Article
Entomology
Joseph L. Black, Gus M. Lorenz, Aaron J. Cato, Nick R. Bateman, Nicholas J. Seiter
Summary: This study examined the effectiveness of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) in controlling different larval instars of the soybean pest Helicoverpa zea, as well as the potential for cross-generational infection. The results showed that HearNPV was effective in controlling 1st-3rd instar larvae, but not 4th or 5th instar larvae. Additionally, the second generation of larvae infected by HearNPV died faster than the first generation, indicating the potential for cross-generational infection. These findings suggest that HearNPV could be a useful tool in managing H. zea.
Article
Biochemistry & Molecular Biology
George-Rafael Samantsidis, Shane Denecke, Luc Swevers, George Skavdis, Sven Geibel, John Vontas
Summary: This study identified functional RNA polymerase II and III promoters in Helicoverpa zea, which can be used for genetic manipulation in the context of agricultural biotechnology. Furthermore, four U6 promoters of H. armigera were also identified and evaluated, showing their potential for methodologies such as RNAi and CRISPR/Cas9.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Tao Dou, Sufen Bai, Futao Gao, Liangheng Tian, Junhua Zhu, Xiaohang Gu, Xifa Yang, Youwu Hao, Shiheng An, Xiangyang Liu, Xinming Yin
Summary: This study found that a combination of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) and Campoletis chlorideae could improve the efficacy of biological control against Helicoverpa armigera. Additionally, HearNPV infection had negative effects on the development and reproduction of Campoletis chlorideae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Environmental Sciences
Zhiqiang Tian, Meng Zha, Limei Cai, J. P. Michaud, Jie Cheng, Zhongjian Shen, Xiaoming Liu, Xiaoxia Liu
Summary: Infection of Helicoverpa armigera single nucleopolyhedrovirus (HearNPV) can restrict the growth and development of larvae by inducing reactive oxygen species (ROS) and affecting immune responses. The transcription factor forkhead box O (FoxO) upregulates the expression of peroxiredoxin 1 (Prx1), which plays a role in regulating larval development and immunity following HearNPV infection.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Multidisciplinary Sciences
Allah Ditta Abid, Shafqat Saeed, Syed Muhammad Zaka, Sohail Shahzad, Muhammad Nadir Naqqash, Naeem Iqbal
Summary: Farmers often rely on chemical control to manage insect pests, but this can pose health hazards for humans and the environment. Biopesticides like Nucleopolyhedrovirus (NPVs) offer a less toxic alternative, with effective control against lepidopteran larvae. This study aimed to determine the host range of NPV in Pakistan. Results showed high mortality in Helicoverpa armigera, the target pest, while other tested species showed no significant difference in mortality compared to the control. Pathogenicity varied with time of infection, influenced by factors such as larval body size and environmental conditions. NPV exhibited high specificity, making it a promising ecofriendly tool for managing H. armigera.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2022)
Article
Cell Biology
Yan-Li Li, Yan-Xue Li, Xiao-Pei Wang, Xin-Le Kang, Ke-Qin Guo, Du-Juan Dong, Jin-Xing Wang, Xiao-Fan Zhao
Summary: This study identified GPCRs that are upregulated at the metamorphic stage in Helicoverpa armigera and confirmed their roles in pupation using RNA interference and binding assays. The results showed that different GPCRs are involved in the regulation of gene expression by 20E hormone, integrating growth and development processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Entomology
Xiao-Lan Liu, Xi Chu, Long-Long Sun, Ya-Nan Wang, Gui-Ying Xie, Wen-Bo Chen, Yang Liu, Bente G. Berg, Shi-Heng An, Gui-Rong Wang, Xin-Ming Yin, Xin-Cheng Zhao
Summary: This study investigated the physiology and morphology of olfactory sensory neurons (OSNs) in male H. armigera, revealing the specific responses and axonal projection areas of OSNs in different sensillae. These findings provide a crucial foundation for further research on insect olfaction in this species.
Article
Biochemistry & Molecular Biology
Zhongyuan Deng, Yakun Zhang, Yalu Li, Kaiyuan Huang, Xuewei Chen, Min Zhang, Jinyong Huang, Xinzhi Ni, Xianchun Li
Summary: The Masc gene in Helicoverpa armigera controls sex development and dosage compensation, with expression influenced by gender and growth stage. Inhibition of HaMasc leads to the appearance of female-specific mRNA splicing isoforms and affects the development of male characteristics in H. armigera.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Leyao Li, Shan Wang, Kaiyuan Huang, Yuting Zhang, Yalu Li, Min Zhang, Jinyong Huang, Zhongyuan Deng, Xinzhi Ni, Xianchun Li
Summary: This study identified and characterized miRNAs in the ovaries and testes of the cotton bollworm, providing insights into the potential roles of these miRNAs in the reproductive processes of this destructive crop pest. The differential expression of gonad-biased miRNAs suggests their involvement in reproduction-related pathways, making them potential targets for reproductive-destruction-based control strategies against Helicoverpa armigera and other lepidopteran pests.
Article
Agronomy
Zhong-Jian Shen, Yan-Jun Liu, Jie Cheng, Zhen Li, J. P. Michaud, Xiao-Xia Liu
Summary: This study found that even short periods of exposure to high temperature can significantly reduce susceptibility of Helicoverpa armigera larvae to a specific virus, HearNPV, by stimulating the production of heat shock proteins (HSPs) which enhance immune responses.
PEST MANAGEMENT SCIENCE
(2022)
Article
Entomology
Sali Li, Zhichao Cao, Yuhong Wu, Rui Tang, Shaolei Sun, Zhen Yang, Tong-Xian Liu, Xiangfeng Jing
Summary: This study focused on the function of DGAT1 in Helicoverpa armigera and found that DGAT1 played important roles in triglyceride synthesis in the midgut and ovaries, as well as in reproduction. This work provides valuable information for understanding lipid metabolism in insects.
ENTOMOLOGIA GENERALIS
(2022)
Article
Biochemistry & Molecular Biology
Kaixia Li, Shan Yu, Yihua Yang, Ya-Zhou He, Yidong Wu
Summary: This study investigates the feeding behavior of cotton bollworm larvae and the underlying mechanisms after exposure to Cry1Ac protein. It is found that the feeding cessation response to Cry1Ac in cotton bollworm larvae is closely associated with midgut epithelial damage and downregulation of NPF and NPFR expression. These findings provide insights into the mechanism of feeding cessation in response to Bt intoxication and the mode of action of the Cry1Ac toxin in target pests.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Xue Yao, Yunpeng Duan, Zhongyuan Deng, Wenli Zhao, Jizhen Wei, Xianchun Li, Shiheng An
Summary: This study confirms the involvement of Helicoverpa armigera ATP synthase subunit a (HaATPs-a) in Cry1Ac resistance and demonstrates the downregulation of HaATPs-a expression in response to Cry1Ac. The interaction between HaATPs-a and Cry1Ac is verified, and purified HaATPs-a is shown to enhance Cry1Ac toxicity to H. armigera larvae.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Virology
Wei Shao, Lihong He, Qingxiu Chen, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Article
Virology
Jun Wang, Minglu Yang, Haibing Xiao, Guo-Hua Huang, Fei Deng, Zhihong Hu
Letter
Cell Biology
Manli Wang, Ruiyuan Cao, Leike Zhang, Xinglou Yang, Jia Liu, Mingyue Xu, Zhengli Shi, Zhihong Hu, Wu Zhong, Gengfu Xiao
Letter
Cell Biology
Jia Liu, Ruiyuan Cao, Mingyue Xu, Xi Wang, Huanyu Zhang, Hengrui Hu, Yufeng Li, Zhihong Hu, Wu Zhong, Manli Wang
Article
Biotechnology & Applied Microbiology
Lihong He, Wei Shao, Jiang Li, Fei Deng, Hualin Wang, Zhihong Hu, Manli Wang
Summary: This study systematically analyzed the nuclear localization of 154 ORFs encoded by AcMNPV, with 25 viral proteins found to be localized in the nucleus in the absence of virus infection. Upon virus infection, 11 proteins originally located in the cytoplasm were completely translocated into the nucleus, suggesting their nuclear import is facilitated by other viral or host proteins. Further experiments identified specific proteins that were imported into the nucleus with the assistance of other nuclear localization proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Tong Chen, Xiaoyan Duan, Hengrui Hu, Yu Shang, Yangbo Hu, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu
Summary: The study investigated the impact of 42 genes of AcMNPV on the production of infectious BVs, revealing that 36 genes are dispensable, 3 genes are essential, and 3 genes are important but not essential for infectious BV production. The results contribute to understanding the functional genomics of baculoviruses and provide valuable information for future engineering of baculovirus expression systems.
Article
Virology
Mingyue Xu, Bo Wang, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu, Jia Liu
Summary: The establishment of a reverse genetics system based on a SFTSV C4 strain has revealed insights into virus replication and protein expression, and provides a valuable tool for the development of vaccines and antivirals against SFTS.
Correction
Virology
Mingyue Xu, Bo Wang, Fei Deng, Hualin Wang, Manli Wang, Zhihong Hu, Jia Liu
Article
Biochemical Research Methods
Hengrui Hu, Kai Pan, Yu Shang, Yijia Guo, Han Xiao, Fei Deng, Manli Wang, Zhihong Hu
Summary: The study utilized synthetic biology to investigate the fundamental function of baculoviral hrs through multiloci manipulation, revealing the importance of retaining a single hr for efficient viral DNA replication and progeny production. The research also identified the role of hrs in enhancing gene transcription within surrounding regions. Overall, the advantages of synthetic biology in engineering and functional studies of large DNA viruses were demonstrated.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Virology
Yu Shang, Hengrui Hu, Xi Wang, Hualin Wang, Fei Deng, Manli Wang, Zhihong Hu
Summary: Researchers successfully modified a synthetic baculovirus, AcMNPV-WIV-Syn1, into a novel bacmid, AcBac-Syn, for use as a backbone for baculovirus system. By constructing a vector containing a LacZ:attTn7 and egfp cassette and recombining it with the AcMNPV-WIV-Syn1 genome in yeast, they were able to generate the bacmid AcBac-Syn. The resulting bacmid showed similar biological characteristics to the wild-type virus and could be easily used for foreign gene insertion and expression.
Article
Virology
Yujia Liu, Xiaoqian Zhang, Dongbin Chen, Dehong Yang, Chenxu Zhu, Linmeng Tang, Xu Yang, Yaohui Wang, Xingyu Luo, Manli Wang, Yongping Huang, Zhihong Hu, Zulian Liu
Summary: This study demonstrates the successful prevention of BmNPV infection by disrupting the lef8 and lef9 genes using the CRISPR/Cas9 system. The replication and mortality of BmNPV were significantly reduced in the transgenic silkworms. These findings provide alternative targets for the CRISPR antiviral system and offer new ideas for virus infection research and insect pest control.
Article
Virology
Huanyu Zhang, Wenhua Kuang, Congcong Fu, Jiang Li, Manli Wang, Zhihong Hu
Summary: The study identified that the baculovirus core gene ac81 likely encodes a disulfide isomerase which plays a crucial role in various functions of the baculovirus life cycle. This suggests that viral disulfide bond formation could be an ancient mechanism shared by insect-specific large DNA viruses like baculoviruses, nudiviruses, and hytrosaviruses encoding AC81 and P33 (sulfhydryl oxidase).
JOURNAL OF VIROLOGY
(2022)
Article
Virology
Yijia Guo, Hengrui Hu, Han Xiao, Fei Deng, Jiang Li, Manli Wang, Zhihong Hu
Summary: In this study, we used synthetic biology to optimize baculovirus expression vectors by reducing the number of genes through gene deletion. We demonstrated that the newly constructed virus is capable of producing infectious progeny viruses.
Article
Virology
Xiao Wang, Mingyue Xu, Huanhuan Ke, Longda Ma, Liushuai Li, Jiang Li, Fei Deng, Manli Wang, Zhihong Hu, Jia Liu
Summary: This study constructed a reporter virus for SFTSV, which can be used for drug screening in vitro. The results showed that this reporter virus can effectively screen antiviral drugs. Additionally, the reporter virus exhibited attenuated pathogenicity in mice, making it a promising tool for high-throughput screening of antiviral drugs in the future.
Letter
Cell Biology
Jia Liu, Yufeng Li, Liang Liu, Xudong Hu, Xi Wang, Hengrui Hu, Zhihong Hu, Yiwu Zhou, Manli Wang