Review
Infectious Diseases
Lei Guo, Li Li, Li Liu, Tiesong Zhang, Ming Sun
Summary: This article provides an overview of the progress and future directions in the research of potent F-specific nMAbs against hMPV, and discusses the strategy for vaccine research and development.
Article
Virology
Xiangyu Huang, Guihu Yin, Yiqin Cai, Jianing Hu, Jingwen Huang, Qingtao Liu, Xiuli Feng
Summary: In this study, three novel neutralizing epitopes were identified in the HA protein of H9N2 avian influenza virus, which could provide valuable insights for the development of treatment strategies and vaccine design.
Article
Cell Biology
Scott A. Rush, Gurpreet Brar, Ching-Lin Hsieh, Emilie Chautard, Jennifer N. Rainho-Tomko, Chris D. Slade, Christine A. Bricault, Ana Kume, James Kearns, Rachel Groppo, Sophia T. Mundle, Linong Zhang, Danilo Casimiro, Tong-Ming Fu, Joshua M. DiNapoli, Jason S. McLellan
Summary: This study provides insights into the humoral immune response to human metapneumovirus (hMPV) infection in older adults, identifying a highly polyclonal response and revealing the recognition mechanisms of neutralizing antibodies against hMPV F protein.
Article
Pharmacology & Pharmacy
Annelies Van Den Bergh, Benjamin Bailly, Patrice Guillon, Mark von Itzstein, Larissa Dirr
Summary: Human metapneumoviruses (HMPV) have emerged as an important global pathogen, causing severe respiratory infections in young children, the elderly, and immunocompromised individuals. Despite the lack of available vaccines and drugs, the fusion protein of HMPV is considered a promising target for antiviral treatment.
ANTIVIRAL RESEARCH
(2022)
Article
Virology
Xiao Xiao, Zhiyun Wen, Qing Chen, Jennifer M. Shipman, James Kostas, John C. Reid, Christopher Warren, Aimin Tang, Bin Luo, Gregory O'Donnell, Arthur Fridman, Zhifeng Chen, Kalpit A. Vora, Lan Zhang, Hua-Poo Su, Michael J. Eddins
Summary: Human metapneumovirus (hMPV) is a major respiratory pathogen worldwide, and there are currently no approved hMPV vaccines or neutralizing antibodies available for therapeutic or prophylactic use. This study reports a novel antibody that targets the trimerization interface of hMPV fusion protein and provides strong lung protection against hMPV A viral challenge. Understanding the immune recognition of antibodies to hMPV-F antigen is crucial for developing effective hMPV monoclonal antibodies and vaccines.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
C. Garrett Rappazzo, Ching-Lin Hsieh, Scott A. Rush, Emma S. Esterman, Teresa Delgado, James C. Geoghegan, Anna Z. Wec, Mrunal Sakharkar, Vicente Mas, Jason S. McLellan, Laura M. Walker
Summary: In this study, the B cell responses to hMPV were investigated using high-throughput single-cell technology, and rare and highly potent broadly neutralizing antibodies targeting specific epitopes were identified. Additionally, monotherapy with neutralizing antibodies targeting different antigenic sites showed effective protection against lower respiratory tract infection. This study provides promising monoclonal antibody candidates for passive immunoprophylaxis and informs the design of hMPV vaccines.
Article
Multidisciplinary Sciences
Margot Miranda-Katz, John J. Erickson, Jie Lan, Alwyn Ecker, Yu Zhang, Sebastian Joyce, John Williams
Summary: The study identified 6 HLA-B7-restricted CD8(+) T cell epitopes in mice infected with HMPV, with M195-203 (M195) eliciting the strongest response. Immunization with pooled HLA-B7-restricted peptides reduced viral titer and protected mice from virulent infection. CD8(+) T cells from HLA-B7 positive humans also recognize the identified epitopes, providing valuable information for future HMPV vaccine design.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Renato Kaylan Alves de Oliveira Franca, Jacyelle Medeiros Silva, Lucas Silva Rodrigues, Dimitri Sokolowskei, Marcelo Macedo Brigido, Andrea Queiroz Maranhao
Summary: This study utilized phage display technology to select human antibodies targeting the fusion loop region of the Zika virus. Three antibodies that can neutralize Zika virus infection were obtained through four rounds of selection. Due to the high conservation of the fusion loop region, these new antibodies have the potential to be used in therapeutic interventions against Zika virus and other flavivirus illnesses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Geyan Liu, Kang Wang, Zhen Yang, Xiaoyu Tang, Yungfu Chang, Ke Dai, Xinwei Tang, Bangdi Hu, Yiwen Zhang, Sanjie Cao, Xiaobo Huang, Qigui Yan, Rui Wu, Qin Zhao, Senyan Du, Xintian Wen, Yiping Wen
Summary: In this study, three monoclonal antibodies 5D11, 2H81, and 4F2 against recombinant HbpA were generated. Among them, 5D11 showed strong binding affinity and can potentially be used for serological diagnostic tools for G. parasuis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Avik Banerjee, Jiachen Huang, Scott A. Rush, Jackelyn Murray, Aaron D. Gingerich, Fredejah Royer, Ching-Lin Hsieh, Ralph A. Tripp, Jason S. McLellan, Jarrod J. Mousa
Summary: This study identified immunodominant antigenic epitopes on the hMPV F protein and identified a monoclonal antibody (mAb) therapy for hMPV F disease prevention and treatment. Additionally, a prefusion-specific epitope on the hMPV F protein was discovered.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Virology
Yufang Yi, Shuxia Wang, Xiaoli Wang, Pei Xiong, Qingwei Liu, Chao Zhang, Feifei Yin, Zhong Huang
Summary: Two distinct blockade antibody epitopes on the GII.3 capsid have been identified, enhancing the understanding of the immunology and evolution of GII.3 noroviruses. The epitopes of the antibodies 8C7 and 8D1 have been mapped to specific residues on the VP1 capsid protein, with potential implications for the development of therapeutic interventions. Structural modeling shows the spatial relationship of these epitopes to the HBGA binding site, providing insights into the mechanism of viral infection.
Article
Virology
Zhiqi Zhang, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: Nervous necrosis virus (NNV) is a significant pathogen in the aquaculture industry, causing substantial financial losses. The capsid protein (CP) is the only structural protein with pathogenicity and antigenicity, but information on immunodominant regions is scarce.
JOURNAL OF VIROLOGY
(2023)
Article
Chemistry, Medicinal
Hua-Rui Gong, Ye-fan Hu, Xuechen Li, Thomas Yau, Bao-Zhong Zhang, Jian-Dong Huang
Summary: This study investigates the infection enhancement effect of a multiple epitope-based vaccine on the Omicron strain of SARS-CoV-2. The results show that while the vaccine enhances infection for Omicron, it does not have the same effect on the ancestral SARS-CoV-2 or Delta strains. Furthermore, the study identifies a conserved epitope that has neutralizing activity against the three strains. This research provides up-to-date epitope information for guiding the development of better vaccines or antibody-based therapies against future variants.
ACS INFECTIOUS DISEASES
(2022)
Article
Immunology
Wenjuan Du, Rick Janssens, Anna Z. Mykytyn, Wentao Li, Dubravka Drabek, Rien van Haperen, Marianthi Chatziandreou, Melanie Rissmann, Joline van der Lee, Melissa van Dortmondt, Itziar Serna Martin, Frank J. M. van Kuppeveld, Daniel L. Hurdiss, Bart L. Haagmans, Frank Grosveld, Berend-Jan Bosch
Summary: A six-antigen binding site biparatopic heavy-chain-only antibody is designed, which shows potent neutralizing activity against SARS-CoV-2 and Omicron variants, and protects against SARS-CoV-2 infection in a hamster model.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Emily Phung, Lauren A. Chang, Maryam Mukhamedova, Lijuan Yang, Deepika Nair, Scott A. Rush, Kaitlyn M. Morabito, Jason S. McLellan, Ursula J. Buchholz, John R. Mascola, Michelle C. Crank, Grace Chen, Barney S. Graham, Tracy J. Ruckwardt
Summary: RSV pre-F vaccination results in expansion and activation of RSV and HMPV F-specific B cells that contribute to long-term pneumovirus immunity.
SCIENCE TRANSLATIONAL MEDICINE
(2022)