Journal
JOURNAL OF GENERAL VIROLOGY
Volume 89, Issue -, Pages 2622-2629Publisher
MICROBIOLOGY SOC
DOI: 10.1099/vir.0.2008/001933-0
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Funding
- National Natural Science Foundation of China [30371278]
- Beijing Natural Science Foundation [5042020]
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The complete genome of a novel adult diarrhoea rotavirus strain J19 was cloned and sequenced using an improved single-primer sequence-independent method, The complete genome is 17 961 bp and is AU-rich (66.49%). Northern blot analysis and genomic sequence analysis indicated that segments 1-11 encode 11 viral proteins, respectively. Protein alignments with the corresponding proteins of J19 with B219, and groups A, B and C rotaviruses, produced higher per cent sequence identities to B219. Among groups A, B and C rotaviruses, 10 proteins from group B rotaviruses exhibited slightly higher amino acid sequence identity to the Jig proteins, but proteins of J19 showed low amino acid sequence identity with groups A and C rotaviruses. Construction of unrooted phylogenetic trees using a set of known proteins and representatives of three known rotavirus groups revealed that six structural proteins were positioned close to B219 and the basal nodes of groups A, B and C lineages, although with a preferred association with group B lineages. Phylogenetic analysis of the five non-structural proteins showed a similar trend. The results of the serological analysis, protein sequence analysis and phylogenetic analysis suggested that J19 would be a novel rotavirus; strain with great significance to the evolution and origin of group B rotaviruses.
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