Article
Fisheries
Nermeen Y. Abass, Rhoda Mae C. Simora, Jinhai Wang, Shangjia Li, De Xing, Michael Coogan, Andrew Johnson, David Creamer, Xu Wang, Rex A. Dunham
Summary: This study demonstrates that genetic enhancement of the channel catfish's resistance to Edwardsiella ictaluri can be achieved through cecropin transgenesis. The transgenic fish exhibited enhanced disease resistance and improved survival time when challenged with the bacteria. The growth rates of transgenic and non-transgenic fish were not different, indicating that the transgene did not affect the overall growth of the fish.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Tan-Trung Nguyen, Tran T. T. Xuan, To H. Ngoc, Le T. My Duyen, Tu Q. Vinh, Pham D. T. My, Hoang A. Hoang, Le P. Nga
Summary: This study reveals the existence of two novel bacteriophages infecting Edwardsiella ictaluri, vB_EiM_PVN06 and vB_EiA_PVN09, originating from the Mekong Delta in Vietnam. These bacteriophages exhibit distinct biological properties and show potential for phage therapy in the control of ESC disease.
Article
Fisheries
Ahmed Abdelmoniem Mousa, Reshma Ramachandran, Ozan Ozdemir, Attila Karsi, Hossam Abdelhamed
Summary: The study showed that dietary trans-cinnamaldehyde (TC) can increase the activity of superoxide dismutase (SOD) and decrease the concentration of malondialdehyde (MDA) in hepatic tissue of channel catfish. Additionally, after infection with Edwardsiella ictaluri, catfish fed with TC demonstrated significantly increased antioxidant defense responses on days 1, 6, and 14 post-infection. However, there were no significant differences in the activities/concentrations of catalase (CAT), reduced glutathione (GSH), nitric oxide (NO), and MDA between the TC and control groups.
Article
Fisheries
Jicheng Yang, Yaoyao Lin, Zhaohui Wei, Zhenbing Wu, Qianqian Zhang, Jingwen Hao, Shuyi Wang, Aihua Li
Summary: 16S rRNA gene high-throughput sequencing technology was used to investigate the changes in the gut microbiota of yellow catfish infected by Edwardsiella ictaluri. The microbial composition and diversity of the diseased fish were significantly different from those of healthy fish. Proteobacteria became the dominant phylum in the gut of the diseased fish, while Cetobacterium, Plesiomonas, and Romboutsia disappeared. The gut microbiota of the diseased fish showed reduced anaerobic and Gram-positive bacteria, as well as decreased digestive system, immune system, and metabolic functions.
Article
Fisheries
Uni Purwaningsih, Sukenda Sukenda, Angela M. Lusiastuti, Alimuddin Alimuddin, Widanarni Widanarni, Sri Nuryati
Summary: This study compared the phenotypic, genotypic characteristics, and pathogenicity of Edwardsiella ictaluri from different catfish farms in Indonesia. The results showed variations in growth patterns, enzyme activity, antibiotic resistance, and genetic profiles among the isolates. The protein components of E. ictaluri cells were found to be virulent and played a role in the pathogenesis of E. ictaluri infection in catfish.
AQUACULTURE RESEARCH
(2022)
Article
Fisheries
Adrian Lopez-Porras, Matt J. Griffin, Cynthia Ware, Bradley M. Richardson, Terrence E. Greenway, Thomas Graham Rosser, Suja Aarattuthodiyi, David J. Wise
Summary: Edwardsiella piscicida is a growing problem in catfish aquaculture in the southeastern United States, especially in channel and hybrid catfish. Multiple phyletic groups of E. piscicida have been identified, with no significant differences in virulence between channel and hybrid catfish. A live-attenuated E. ictaluri vaccine has shown cross-protection against one E. piscicida isolate, but its effectiveness against other variants is unknown. Exposure to heterologous E. piscicida isolates improves survival following E. ictaluri challenge, suggesting the presence of shared and conserved antigens.
JOURNAL OF FISH DISEASES
(2022)
Article
Geriatrics & Gerontology
Vladimir F. Lazarev, Magda Tsolaki, Elena R. Mikhaylova, Konstantin A. Benken, Maxim A. Shevtsov, Alina D. Nikotina, Mirna Lechpammer, Vladimir A. Mitkevich, Alexander A. Makarov, Alexey A. Moskalev, Sergey A. Kozin, Boris A. Margulis, Irina Guzhova, Evgeny Nudler
Summary: The study revealed that the interaction between glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and amyloid-beta (A beta) contributes to neurodegeneration in Alzheimer's disease (AD) patients. By investigating the relationship between A beta and GAPDH, potential therapeutic targets were identified through reversing the pathogenic abnormalities associated with AD.
Article
Fisheries
Nguyen Van Sang, Tran Thi Phuong Dung, Vo Hong Phuong, Nguyen Hong Nguyen, Nguyen Huu Thinh
Summary: The present study analyzed the immune responses of resistant (RF) and susceptible families (SF) of striped catfish (Pangasianodon hypophthalmus) to Edwardsiella ictaluri, the causative agent of bacillary necrosis of Pangasius. The RF group showed a more effective non-specific immune response compared to the SF group. White blood cell counts increased significantly in the RF group, starting from 48 hours post infection (hpi), while the increase was slower in the SF group, mainly after 264 hpi. Monocyte and neutrophil development was faster in the RF families, and the number of melano-macrophage centers was higher in the RF group at 24-48 hpi. The RF group also exhibited a higher specific immune response (antibody titer) compared to the SF group. The study identified the critical period of 24-48 hpi for differentiating disease resistant individuals, with an Area Under the Curve (AUC) value of 0.95.
Article
Oncology
Congle Shen, Weijie Li, Yajie Wang
Summary: This study analyzed the expression and oncogenic processes of the internal reference gene GAPDH in various cancers. The results showed that GAPDH was highly expressed in the majority of TCGA tumors and its overexpression predicted poor survival. Moreover, GAPDH expression was negatively correlated with immune infiltration. Therefore, GAPDH may be a promising prognostic biomarker for pan-cancer.
TRANSLATIONAL CANCER RESEARCH
(2023)
Article
Fisheries
Arthur Roberto da Costa, Diego Candido de Abreu, Roberta Torres Chideroli, Kauane Mileide de Souza do Espirito Santo, Daniela Dib Goncalves, Giovana Wingeter Di Santis, Ulisses de Padua Pereira
Summary: Infections caused by Edwardsiella ictaluri are a major issue in the catfish industry in North America, with a study in Brazil showing susceptibility of Pseudoplatystoma corruscans to this bacterium and the potential for interspecies transmission. The research revealed high mortality rates in challenged pintados and cohabiting species, highlighting the importance of understanding and managing infections in aquatic environments.
DISEASES OF AQUATIC ORGANISMS
(2021)
Article
Immunology
Xu Zhou, Gui-Rong Zhang, Wei Ji, Ze-Chao Shi, Xu-Fa Ma, Zun-Lan Luo, Kai-Jian Wei
Summary: The study revealed that the immune response of yellow catfish against E. ictaluri infection is mainly manifested in the spleen and liver, with key gene expression peaking at 72 hours post-infection. Cytokines were continuously transcribed in the spleen, while the liver mainly secreted components of the complement system, and metabolic regulation was activated from 12 to 120 hours post-infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yejin Jung, Naotaka Noda, Junichiro Takaya, Masahiro Abo, Kohei Toh, Ken Tajiri, Changyi Cui, Lu Zhou, Shin-ichi Sato, Motonari Uesugi
Summary: The discovery of a covalent inhibitor F8 through activity-based proteomic screening is described, where F8 forms a covalent adduct with an aspartic acid in the active site of GAPDH to displace NAD(+) and enhance the probe reaction with the catalytic cysteine. This mechanistic understanding led to the identification of an optimized aspartate-reactive GAPDH inhibitor.
ACS CHEMICAL BIOLOGY
(2022)
Article
Biology
Marios L. Tringides, Zhemin Zhang, Christopher E. Morgan, Chih-Chia Su, Edward W. Yu
Summary: We have developed a new cryo-EM method that can simultaneously determine the atomic resolution structures of multiple proteins. Using this method, we have identified the composition of a human brain microsomal lysate and solved the structures of five proteins related to neurodegenerative diseases. This highlights the importance of cryo-EM in facilitating tissue and organ proteomics at the atomic level.
LIFE SCIENCE ALLIANCE
(2022)
Article
Cell Biology
Qi Wang, Lina Lu, Ming Zeng, Dan Wang, Tian-ze Zhang, Yi Xie, Shi-bo Gao, Shuai Fu, Xue-ping Zhou, Jian-xiang Wu
Summary: The main capsid protein of rice black-streaked dwarf virus (RBSDV), P10, interacts with GAPDH to induce autophagy in Laodelphax striatellus. This study elucidates the molecular mechanism by which RBSDV induces autophagy through the AMPK-GAPDH pathway.
Article
Biochemistry & Molecular Biology
Shen-Li You, Xin-Xin Jiang, Gui-Rong Zhang, Wei Ji, Xu-Fa Ma, Xu Zhou, Kai-Jian Wei
Summary: This study cloned and identified the open reading frame sequences of TRAF genes in yellow catfish and found that these genes play important roles in immune response and may be involved in the immune response against bacterial infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)