Article
Ecology
Thea O. Andersen, Ianina Altshuler, Arturo Vera-Ponce de Leon, Juline M. Walter, Emily McGovern, Kate Keogh, Cecile Martin, Laurence Bernard, Diego P. Morgavi, Tansol Park, Zongjun Li, Yu Jiang, Jeffrey L. Firkins, Zhongtang Yu, Torgeir R. Hvidsten, Sinead M. Waters, Milka Popova, Magnus o. Arntzen, Live H. Hagen, Phillip B. Pope
Summary: Protozoa, including entodiniomorphs and holotrichs, play a major role in the microbial biomass of the rumen microbiome. However, their specific biological and metabolic contributions to rumen function have not been well described. By using metaproteomics, this study reveals the specific metabolic niches occupied by protozoa in relation to their microbial co-habitants in the rumen fluid of cattle and goats fed varying diets.
Article
Biotechnology & Applied Microbiology
Alessandro Vastolo, Roberta Matera, Francesco Serrapica, Monica I. Cutrignelli, Gianluca Neglia, Dieu Donne Kiatti, Serena Calabro
Summary: This study evaluated the fermentation characteristics and kinetics of different types of liquid feed using bovine and buffalo rumen liquor as inocula. The results showed that supplementing liquid feed improved fermentation and had no negative effects.
FERMENTATION-BASEL
(2022)
Article
Microbiology
Yabo Zhao, Yanmei Zhang, Erdene Khas, Changjin Ao, Chen Bai
Summary: The objective of this study was to evaluate the effect of Allium mongolicum Regel ethanol extract on the concentration of three branched-chain fatty acids (BCFAs) in the rumen of lambs. The results showed that the extract decreased the content of certain fatty acids and altered the fermentation parameters and bacteria composition in the rumen.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Solene Fresco, Christel Marie-Etancelin, Annabelle Meynadier, Guillermo Martinez Boggio
Summary: This study analyzed the stability of bacterial composition and its associations with rumen and milk fatty acids over a 1-week period. Results showed significant changes in bacterial composition over the week, but some operational taxonomic units (OTUs) were highly correlated with fatty acid phenotypes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Yi Xiong, Chunze Guo, Lin Wang, Fei Chen, Xianwen Dong, Xiaomei Li, Kuikui Ni, Fuyu Yang
Summary: PMS30 and PMS40 increased dry matter intake and average daily gain in Hu lambs, with higher levels of C24:1 and C20:5n-3. PMS40 led to an increase in microbial protein (MCP) in rumen, but PMS30 and PMS40 reduced total volatile fatty acids. Furthermore, PMS40 increased unsaturated fatty acids in longissimus dorsi muscle through changes in ruminal microbial composition.
FERMENTATION-BASEL
(2021)
Article
Multidisciplinary Sciences
Lais L. da Cunha, Hugo F. Monteiro, Caio C. Figueiredo, Igor F. Canisso, Rodrigo C. Bicalho, Felipe C. Cardoso, Bart C. Weimer, Fabio S. Lima
Summary: Comparing the rumen contents collected by oro-esophageal tubing and rumen cannula, it was found that there were slight differences in the microbiome and metabolome. However, the overall microbiome composition and diversity were similar between the two methods. This suggests that oro-esophageal sampling can be used as a proxy to study the rumen microbiome compared to the traditional rumen cannula technique.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
G. Neglia, A. Cotticelli, A. Vassetti, R. Matera, A. Staropoli, F. Vinale, A. Salzano, G. Campanile
Summary: The use of green feed for livestock breeding is an important strategy to meet the increasing demand for animal products and consumer concerns about animal welfare and sustainability. This study compared different feeding strategies in lactating water buffaloes using a metabolomic approach. The results showed that there were significant differences in the accumulation of metabolites in milk and rumen fluid samples under different feeding strategies, and some of the natural compounds detected were considered health-promoting nutrients.
SCIENTIFIC REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Javad Gharechahi, Sajjad Sarikhan, Jian-Lin Han, Xue-Zhi Ding, Ghasem Hosseini Salekdeh
Summary: Rumen microbiota plays an important role in the digestion and metabolism of lignocellulosic substrates, and our study on the camel rumen revealed distinct functional capabilities that enable different ruminant species to thrive on various plant substrates.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Multidisciplinary Sciences
Mengwei Li, Faizul Hassan, Lijuan Peng, Huade Xie, Xin Liang, Jiaxiang Huang, Feng Huang, Yanxia Guo, Chengjian Yang
Summary: This study demonstrates that mulberry leaf flavonoids can modulate the composition of rumen microbiota in water buffalo, improving nutrient digestibility. A dose of 45 g/d was found to be effective.
Article
Agriculture, Dairy & Animal Science
M. Baldin, D. Garcia, G. I. Zanton, F. Hao, A. D. Patterson, K. J. Harvatine
Summary: This experiment aimed to verify the effect of HMTBa on biohydrogenation-induced milk fat depression (MFD) and investigate its impact on rumen environment and fermentation. The results showed that HMTBa could reduce the concentration of trans fatty acids in milk fat, increase milk fat yield, and also affect rumen environment and digestion processes.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agronomy
Yiguang Zhao, Yue Wang, Xuemei Nan, Linshu Jiang, Yapin Wang, Jun Liu, Junhu Yao, Md Tanvir Rahman, Benhai Xiong
Summary: The study found that inulin supplementation in dairy cow feed increased milk production, changed milk fatty acid profiles, elevated rumen volatile fatty acid concentration, and enhanced antioxidant and immunity function in a dose-dependent manner.
Article
Biochemistry & Molecular Biology
Xi Luo, Shihai Huang, Mingming Liang, Qingsong Xue, Saif Ur Rehman, Xuan Ren, Yanfang Li, Ting Yang, Deshun Shi, Xiangping Li
Summary: This study investigates the impact of succinylation on sperm freezability and suggests that lysine succinylation can potentially influence the sperm freezability of Mediterranean buffaloes through mitochondrial lipid metabolism.
Article
Food Science & Technology
Pei Nie, Bin Pan, Muhammd Jamil Ahmad, Xinxin Zhang, Chao Chen, Zhiqiu Yao, Haimiao Lv, Ke Wei, Liguo Yang
Summary: The study compared basic chemical parameters, fatty acids, amino acids, and lipid quality indices of crossbred buffalo milk produced in summer and winter, revealing that summer buffalo milk is more health-beneficial.
Article
Microbiology
Boxue Si, Kaizhen Liu, Guoxin Huang, Meiqing Chen, Jiyong Yang, Xufang Wu, Ning Li, Wenhao Tang, Shengguo Zhao, Nan Zheng, Yangdong Zhang, Jiaqi Wang
Summary: The variation of milk fat is closely related to rumen bacteria, and certain rumen bacteria have a potential impact on the deposition of specific fatty acids in raw milk.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Na T. D. Tran, Apisit Chaidee, Achirawit Surapinit, Manachai Yingklang, Sitiruk Roytrakul, Sawanya Charoenlappanit, Porntip Pinlaor, Nuttanan Hongsrichan, Sirirat Anutrakulchai, Ubon Cha'on, Somchai Pinlaor
Summary: This study investigated the impact of chronic Strongyloides stercoralis infection on the gut microbiome and microbial activity. The results showed that chronic infection led to the over-representation of the Ruminococcus torques group and alterations in the microbial proteome, specifically in carbohydrate metabolism mediated by Bacteroidaceae.
SCIENTIFIC REPORTS
(2023)
Article
Microbiology
Sharon A. Huws, Joan E. Edwards, Wanchang Lin, Francesco Rubino, Mark Alston, David Swarbreck, Shabhonam Caim, Pauline Rees Stevens, Justin Pachebat, Mi-Young Won, Linda B. Oyama, Christopher J. Creevey, Alison H. Kingston-Smith
Summary: The study investigated temporal ecological interactions at the plant-biofilm interface in the rumen, identifying two distinct sub-microbiomes and key bacterial families with high transcriptional activity. It also highlighted potential keystone taxa that may be 'cheating' to capitalize on energy-harvesting capacity of others. The findings provide valuable insights for potential plant breeding strategies aimed at sustainable ruminant production.
Article
Microbiology
Nathaly Cancino-Padilla, Natalia Catalan, Karen Siu-Ting, Christopher J. Creevey, Sharon A. Huws, Jaime Romero, Einar Vargas-Bello-Perez
Summary: This study investigated the long-term effects of saturated and unsaturated fatty acid sources on the rumen microbiome of dairy cows. Results showed that supplementation of 3% dry matter lipid over 63 days did not have major impacts on the global bacterial community structure. Firmicutes and Bacteroidetes were the dominant phyla in the rumen microbiota.
Article
Food Science & Technology
Panagiotis Sapountzis, Serafino Teseo, Saria Otani, Frank Moller Aarestrup, Evelyne Forano, Garett Suen, George Tsiamis, Bradd Haley, Jo Ann Van Kessel, Sharon A. Huws
Summary: Animal husbandry is crucial for the sustainability of human societies, with research on intestinal microbiota playing a key role in combating foodborne and animal pathogens, reducing antimicrobial resistance, and potentially improving animal welfare and nutrition. The Fecobiome Initiative (FI) is an international effort aiming to facilitate collaboration on research projects, disseminate research results, and increase public availability of resources for this important area of study.
FOODBORNE PATHOGENS AND DISEASE
(2022)
Article
Multidisciplinary Sciences
Ana C. M. Vitor, Alexandra E. Francisco, Joana Silva, Mario Pinho, Sharon A. Huws, Jose Santos-Silva, Rui J. B. Bessa, Susana P. Alves
Summary: The study demonstrates that FD N. oceanica biomass is a natural rumen-protected source of EPA for ruminants, increasing EPA concentration in the rumen and affecting the ruminal biohydrogenation of C18 fatty acids.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Sara E. Pidcock, Timofey Skvortsov, Fernanda G. Santos, Stephen J. Courtney, Karen Sui-Ting, Christopher J. Creevey, Sharon A. Huws
Summary: Anaerobic gastrointestinal microbiomes, especially in the rumen, are dominated by Butyrivibrio and Pseudobutyrivibrio, playing a crucial role in energy harvesting. Despite the current classification of six species within these genera, pangenome analysis suggests the presence of 42 species within 32 genera, indicating a high level of genomic variation and incorrectly assigned taxonomy. Additionally, the study reveals a wide range of carbohydrate-active enzymes and a shared evolutionary history among strains of both Butyrivibrio and Pseudobutyrivibrio.
MICROBIAL GENOMICS
(2021)
Article
Agronomy
Piera Iommelli, Federico Infascelli, Nadia Musco, Micaela Grossi, Maria Ferrara, Fiorella Sarubbi, Biagio D'Aniello, Pietro Lombardi, Raffaella Tudisco
Summary: This study compared the effects of two different diets on buffalo meat quality and SCD gene expression in adipose tissue, revealing important differences between buffalo and bovine species, highlighting the need for specific research on buffalo.
Article
Microbiology
Isabela Maria Fernandes de Oliveira, Fernanda Godoy-Santos, Linda Boniface Oyama, Sofia Magalhaes Moreira, Rodrigo Goncalves Dias, Sharon Ann Huws, Christopher J. Creevey, Hilario Cuquetto Mantovani
Summary: This study characterized the genetic features of Streptococcus lutetiensis through whole-genome sequencing, revealing its antimicrobial activity and adaptation to the rumen environment. The genome of S. lutetiensis UFV80 contained a putative CRISPR-Cas9 system, while all bacterial genomes harbored genetic clusters related to the biosynthesis of bacteriocins. SDS-PAGE confirmed the predicted class II bacteriocins. These findings highlight the importance of this research.
Article
Microbiology
Katie Lawther, Fernanda Godoy Santos, Linda Boniface Oyama, Francesco Rubino, Steven Morrison, Chris J. Creevey, John W. McGrath, Sharon Ann Huws
Summary: This study reveals the diverse and abundant antimicrobial resistance gene reservoirs present in both livestock and soil microbiomes, highlighting the significant risks to human and animal health. The analysis of a large number of samples from various countries provides a global insight into resistomes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Linda B. Oyama, Hamza Olleik, Ana Carolina Nery Teixeira, Matheus M. Guidini, James A. Pickup, Brandon Yeo Pei Hui, Nicolas Vidal, Alan R. Cookson, Hannah Vallin, Toby Wilkinson, Denise M. S. Bazzolli, Jennifer Richards, Mandy Wootton, Ralf Mikut, Kai Hilpert, Marc Maresca, Josette Perrier, Matthias Hess, Hilario C. Mantovani, Narcis Fernandez-Fuentes, Christopher J. Creevey, Sharon A. Huws
Summary: This study identified two antimicrobial peptides, HG2 and HG4, from a rumen microbiome metagenomic dataset. These peptides showed activity against methicillin-resistant Staphylococcus aureus (MRSA) strains, including anti-biofilm and anti-inflammatory activities. In addition, they demonstrated low toxicity to human cells and were effective in a mouse model of MRSA infection.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Agronomy
Francesco Serrapica, Felicia Masucci, Giuseppe De Rosa, Ada Braghieri, Fiorella Sarubbi, Francesca Garofalo, Fernando Grasso, Antonio Di Francia
Summary: An observational case study highlighted the issues associated with expanding dairy buffalo farming outside traditional areas in southern Italy. The study found that temperature, reproductive characteristics, and the specific process of mozzarella production are the main challenges that need to be addressed.
Article
Biotechnology & Applied Microbiology
Adam J. Mulkern, Linda B. Oyama, Alan R. Cookson, Christopher J. Creevey, Toby J. Wilkinson, Hamza Olleik, Marc Maresca, Giarla C. da Silva, Patricia P. Fontes, Denise M. S. Bazzolli, Hilario C. Mantovani, Bamu F. Damaris, Luis A. J. Mur, Sharon A. Huws
Summary: This study evaluated the therapeutic potential of antimicrobial peptides (AMPs) from the rumen microbiome against Pseudomonas aeruginosa infections. All AMPs exhibited antimicrobial activity against the strains tested, with no decrease in activity. The AMPs Lynronne 1 and 2 showed efficacy in an in vivo infection model. These findings highlight the potential of microbiome-derived AMPs for the treatment of Pseudomonas aeruginosa infections.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Agriculture, Dairy & Animal Science
Fabio Zicarelli, Fiorella Sarubbi, Piera Iommelli, Micaela Grossi, Daria Lotito, Pietro Lombardi, Raffaella Tudisco, Federico Infascelli, Nadia Musco
Summary: The nutritional quality of forage strongly affects the performance and health of ruminants. Analyzing forage characteristics accurately and timely is crucial for formulating adequate animal rations. Near Infrared Spectroscopy (NIRS) technology provides a fast and accurate alternative to traditional laboratory analysis. This trial analyzed over 400 hay samples from the Campania region in Italy using portable NIRS, revealing significant differences in hay quality among different areas and highlighting the usefulness of NIRS for analyzing feed quality and formulating appropriate rations.
Article
Agriculture, Dairy & Animal Science
Aaron J. Brown, Gillian Scoley, Niamh O'Connell, Alan Gordon, Katie Lawther, Sharon A. Huws, Steven J. Morrison
Summary: Good management of hygiene is required to minimise the risk of disease transmission in pre-weaned calf rearing facilities. In this study, various samples were collected from calf rearing facilities and analysed for hygiene indicators. The results showed that the use of automatic feeders and group pen buckets improved hygiene levels, while self-fill drinkers and single pens had higher chances of containing high levels of bacteria. Cleaning milk feeders after every feed and using group pens with solid floors were also found to be effective in maintaining hygiene levels.
Article
Agronomy
Fabio Zicarelli, Fiorella Sarubbi, Piera Iommelli, Micaela Grossi, Daria Lotito, Raffaella Tudisco, Federico Infascelli, Nadia Musco, Pietro Lombardi
Summary: Knowing the chemical composition of forages and feeds is crucial for formulating balanced rations for ruminants. In this study, samples of corn silage grown in different locations in South Italy were collected and analyzed using NIRS. The nutritional characteristics of the analyzed samples were significantly affected by the area of cultivation/storage.
Review
Biotechnology & Applied Microbiology
Piera Iommelli, Fabio Zicarelli, Nadia Musco, Fiorella Sarubbi, Micaela Grossi, Daria Lotito, Pietro Lombardi, Federico Infascelli, Raffaella Tudisco
Summary: This review investigated the protein degradability of different processed cereals and legumes in ruminants based on the analysis of available literature. Results showed that the majority of the papers focused on energy aspects, and most of the studies in the literature are quite old. Furthermore, there is a high variability of experimental conditions, making it difficult to compare the different results.
FERMENTATION-BASEL
(2022)
